XB-CLONE-3637785
Clone Name: IMAGE:7662031
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Gene:
trim33
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121983 [+]
GTCAGGTCGCCGTGGCCGTTAGAGCGAAGCAAGGGAAAGAGGGGCGGCGTGCTGTACGTGCGCTGGTGGCGTAGCGAAGCGGTCAGTTCAGGGGTTCAAA
GAGGAGAAAACATGGCGGATAACAAAGGAGGAGGTGGCGGTGGGGGAGAGACCGAGGGGGACGCGAGCAGCAATAATAACAACAACAGCAATGGCGCCGG
GCAGACCGGCAGTTCTGGTGACACAGAGAGTCAAACAGTAGAGGAGCCGGAGAGTGTTGAGAAGGCCCCAGCTACTGCGGTCCCTACAGATGCCCCGTCT
GAGGAAAACCCTGCCCCGTCCTCTTCGTCAGTGGCCTCATCTTCTGCTGCTCCAGCACCAACATCATCATCTTCTCCTCTAGTGGTGAACTTACTGGATA
CGTGCGCTGTGTGTAAGCTGAGCCTGCAAAGCCGGGACTCTGAGCCAAAGCTCCTGCCATGTTTGCACTCGTTCTGTCGCCGCTGTCTGCCGGAGCCCGA
GAGGCAGCTCAGTGTTCCCCTACCTGGTGGTACTAATGGAGACATCCAGCAAGTTGGTGTAATAAGGTGCCCAGTATGCAGACAAGAATGCAGGCAGATT
GATCTAGTGGATAACTATTTTGTAAAAGACACTTCAGAAGTACCAAGCAGCACAGATGAAAAGTCTGAACAGGTTTGTACAAGTTGTGAAGATAATGCAA
GTGCGGTTGGGTTTTGTGTGGAATGTGGAGAATGGCTCTGCAAGACATGCATAGAAGCCCATCAAAGGGTTAAATTCACAAAAGACCACATAATAACGAA
GAAAGAAGATGTATCCTCAGAATCAGTGGGAGCTTCAGGTCAACGTCCAGTTTTTTGTCCGGTTCACAAACAAGAGCAACTCAAACTATTTTGCGAAACG
TGTGATAGACTGACATGCAGAGACTGTCAGTTACTAGAGCACAAGGAACACAGGTATCAGTTTCTGGAAGAGGCTTTTCAAAACCAGAAAGGTGCTATTG
AAAACTTACTTGCCAAGCTTCTAGAAAAGAAGAACTATGTACATTTTGCTGCTACTCAGGTTCAAAACAGGATAAAAGAAGTAAATGAAACCAATAAACG
CGTGGAACAGGAAATTAAAGTGGCAATTTTCACTTTAATAAATGAAATTAATAAGAAGGGCAAATCTCTTCTGCAGCAGTTGGAGAGTGTTACAAAAGAA
CGGCAGATTAAACTTGTACAGCAGCAAAATGATATCACTGGTCTCTCACGGCAAATAAAGCATGTCATGACCTTCACTAACTGGGCTATTGCAAGTGGCA
GCAGCACAGCCTTACTGTACAGCAAACGCTTGATCACTTTTCAGTTACGTCATATCTTAAAGGCACGTTGTGACCCTGTCCCAGCAGCCAATGGGGCAGT
AAGATTTCATTGTGACCCAACTTTTTGGGCAAAGAATGTTGTAAATTTAGGTAACCTTGTAATCGAAAATAAGCCAACTGCTGGTTTTACTCCTAATGTC
GTAGTTGGGCAAGTTCCCCAAGGAGTTAACCATGTAAACAAACCACCCGCACAAATTAATTTAGCTCAGCTCCGACTCCAGCATATGCAGCAGCAGGTGT
ATGCTCAAAAACAGCAGCAACTTCAAATGAGGATGGTCCAGCCACCTGTCCAGCATCAGAGACCACCTGCACCTCAAGTAATGCATCAACAGCCTCCAAG
GTTAATAAGCATGCAGAATATGCCAAGGAATAATATGAACTGTGGCCCATTTCAAGCACATCAAATGAGAATGGCACAAAATGCAGCCCAGAATGCTTCC
AGGATGCCTGGTATCCCTCGTCATAATGGACCCCAATACTCAATGATGCAGCCTCATCTCCAAAGACAACAGCATTCGAACCCAGGGCATGCTGGACCAT
TCCCAGTTGTTTCAGTACACAACAACACAATCAATCCAACTAGTCCTACAACCGCAACAATGGCAAATGCAAATCGTGGACCAACCAGCCCATCTGTTAC
ATCTATAGAACTTATACCATCAGTAACAAACCCAGAAAATCTTCCCTCTCTTCCAGACATTCCACCAATTCAGCTGGAAGATGCAGGCTCTAGCACCTTA
GACAATCTATTGAGTAGATATATTTCAATGGGACATCAGCTACCACAACCAACAAGCACTATGAATCCCTCCCCTGCTCCTTCTGCAATGTCTCCGGGAT
CTACAGATGCACTTGAAATGTGTTCCCACTTGCCATGTACACGGTGATGCTAAGAATCTGTACTGTTCCTGTTCTCAGGCTTGTCCAATTCACACACACC
AGTGAGGCCTCCCAGTACATCAAGTACAGGCAGTAGAGGAAGCTGTGGTTCAAGTGGACGAACAATTGAAAGAAACTCTGCCTTTAAATCTGAATCTGTC
AAGGTTAAACAAGAACCTGGAACAGAGGAGGATGTGTGTAGTTTCTCAGGACCAGTAAAGCAGGAAAAGGCTGAAGATGGCAGGAGGAGTGCATGCATGC
TGAGCAGCCCAGAAAGCAGTCTGACACCTCCACTCACCACCAATGTGCACCTTGAAACTGATCTGGAGTCACTGGCCGCTTTGGAAAATCACGTGAAAAC
CGAACCAAACAGCACAAGCCAAAGCTGCAAACAGTCATCTCATGTTGGCCTTGTAAATGGGAAATCCCCTGTACGGAGTTCAATGCACCGGTCACCTAGA
GGAAATGGTGGTGATGGTTCTAACAAAGATGATGACCCCAATGAGGATTGGTGTGCAGTGTGCCAAAATGGAGGAGACCTCTTATGTTGTGAAAAGTGCC
CTAAAGTTTTTCACCTTACTTGTCATGTACCCACGCTGCTTAGTTTTCCCAGTGGTGAATGGATTTGTACGTTCTGCAGAGATCTGAATAAACCTGAAGT
TGAGTATGACTGTGATAATTCTCAGCACAGTAAGAAAGGGAAAACTGTGCAAGGCTTAAGTCCTGTAGACCAAATGAAATGTGAGCGGCTTCTACTTTAT
CTCTACTGTCATGAACTGAGCATTGAATTTCAGGAGCCTGTGCCAGCATCGATACCAAATTACTACAAAATAATTAAGAAGCCAATGGATCTTTCTACTG
TGAAAAAGAAGCTACAGAAGAAACATTCACAACACTATCAAACGCCAGAAGATTTTGTTGCAGATGTCAGGCTAATATTTAAGAACTGCGAAAGGTTCAA
TGAAATGATGAAAGTTGTTCAAGTTTATGCAGAAACACAAGAAATTAATCTACAGAATGACTCAGAAGTTGCCCAAGCAGGGAAGGCTGTTGCAATATAT
TTTGAGGACAAACTTCCTGAAATCTACCCGGACAGGACCTTCCAGCCGTTGCCAGAGTTTGAACCAGAGGATGATGATGGAGATGTAACAGAGGATTCAG
ATGACGATGATTTTGTTCAACCTCGTAGAAAACGACTGAAGTCAGACGAGAGACCGGTGCATATAAAGTGATAAGAGCACGGAACTCTAAAGATTTTGTG
TAGAGACTAAACAAATTTAAAAAGAACTTATTTAAATGTTTCCGGAATATCATCACTCCTGCATCAGCCATCTTCATGAAGAATTTGGCAGCTTTTAGAC
AGTATTAGATCAACAAATATTTGTACAGATAAAGAGCAATCTTTCAAGAAAAGAATTGGGGAAATTTGTCACTTTTTTTTTTTTTTAGGACTGATGGACT
CCTGACCTGGGGAGCAATTCTTGTGTTTTCTCAATTAGGACTTTGTGAATGTTAAAGACAGTCTTTGTTTTTGTTTTGTTTTTTTTCTCCCCCCCAGCAG
TTCTTTGTACAGATGTATAAGTGAACTCGGTGATTGTGTTTCTTGTTGATCTTTCTTTGTTGAACATTGGACCATTACCTTCAGTTTACTGTAATATATT
TCCTTTCCCTGCCAATGAACTGCTCTAAGCTTGATGGCAAAAATGGTACATTAAGTGTACTTTGAAATGGCTTCATCATTCTTCTTTACAGCGTTTTTTT
TTAATTATTTTTGAGCTTTTACATTTCTTGATTGATTATCGGGATTGGATTGTCCTTCAAGACTTTGAAATATATTTATATATTTTGTGAAGGAATCGGT
TTATCATGGTCAAAACTATAGTAACTTGCTTTGTAAATAGAAGGAAGCTTCTCTGTGTTCATATATTCATTATGCATTAATTTGGGCAACAGGTTGTCAA
CTTTTACTGTTGTCTTGCATCTGACATGAGGAAAAATAAATGAATATCTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX843597 [+]tcaggtcgccgtggccgttagagcgaagcaagggaaagaggggcggcgtgctgtacgtgcgctggtggcgtagcgaagcggtcagttcaggggttcaaag
aggagaaaacatggcggataacaaaggaggaggtggcggtgggggagagaccgagggggacgcgagcagcaataataacaacaacagcaatggcgccggg
cagaccggcagttctggtgacacagagagtcaaacagtagaggagccggagagtgttgagaaggccccagctactgcggtccctacagatgccccgtctg
aggaaaaccctgccccgtcctcttcgtcagtggcctcatcttctgctgctccagcaccaacatcatcatcttctcctctagtggtgaacttactggatac
gtgcgctgtgtgtaagctgagcctgcaaagccgggactctgagccaaagctcctgccatgtttgcactcgttctgtcgccgctgtctgccggagcccgag
aggcagctcagtgttcccctacctggtggtactaatggagacatccagcaagttggtgtaataaggtgcccagtatgcagacaagaatgcaggcagattg
atctagtggataactattttgtaaaagacacttcagaagtaccaagcagcacagatgaaaagtctgaacaggtttgtacaagttgtgaagataatgcaag
tgcggttgggttttgtgtggaatgtggagaatggctctgcaagacatgcatagaagctcatcaaagggttaaattcacaaaagaccacataataacgaag
aaagaagatgtatcctcagaatcagtgggagcttcaggtcaacgtccagttttttgt
3' EST: CX843596 [+]aaccagatattcatttatttttcctcatgtcagatgcaagacaacagtaaaagttgacaacctgttgcccaaattaatgcataatgaatatatgaacaca
gagaagcttccttctatttacaaagcaagttactatagttttgaccatgataaaccgattccttcacaaaatatataaatatatttcaaagtcttgaagg
acaatccaatcccgataatcaatcaagaaatgtaaaagctcaaaaataattaaaaaaaaacgctgtaaagaagaatgatgaagccatttcaaagtacact
taatgtaccatttttgccatcaagcttagagcagttcattggcagggaaaggaaatatattacagtaaactgaaggtaatggtccaatgttcaacaaaga
aagatcaacaagaaacacaatcaccgagttcacttatacatctgtacaaagaactgctgggggggagaaaaaaaacaaaacaaaaacaaagactgtcttt
aacattcacaaagtcctaattgagaaaacacaagaattgctccccaggtcaggagtccatcagtcctaaaaaaaaaaaaaagtgacaaatttccccaatt
cttttcttgaaagattgctctttatctgtacaaatatttgttgatctaatactgtctaaaagctgccaaattcttcatgaagatggctgatgcaggagtg
atgatattccggaaacatttaaataagttctttttaaatttgtttagtctctacacaaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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