XB-CLONE-3637556
Clone Name: IMAGE:7662015
|
Gene:
man2b2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC154722 [+]
GAGAATGCTGCTGGGGAACTGGCAGTAAGTTTGAACATGATCGTCCTGCTTGTGCTTCTCTCTGCCTGGGGATGTGTCTGCACTCACTCTGCTCAACCCA
TCCAGGCTTTTGTGATTCCCCACAGCCACATGGATGTGGGATGGGTGTACACAGTACAGGAAAGCATGCACGCATATGCTGCAAATGTCTACACTACGGT
TGTGGAAGAACTGATGAGAGGGGACCGCAGGAGGTTTATAGTGGTGGAACAGGAGTTTTTCCGCTTGTGGTGGAAGTTGTTTGCTGGAAATGTAGAAAAG
CAGCAGGTGCATCAGCTAATGCGTGAAGGAAGGCTAGAGTTTGTTATTGGAGGCCAAGTAATGCACGATGAAGCCCTCACTACGATAGATGATCAGATTT
TACAGCTAACAGAAGGACATGGTTTTATTTATGAAACGTTTGGAGTCAGGCCTCGTTTCTCATGGCATGTGGATCCTTTTGGGGCCTCGGCTGCGTCCCC
TACTTTGTTTGCCATGGCTGGATTTAATGGTCACCTCATCTCCCGAATTGACTACGACACCAAAGCCGACATGCAAAATGGCAAGGAGCTGCAGTTTATC
TGGAGAGCCTCTCCATCCTTATCTGACAAACAAGAGATCTTTACCCATGTAATGGATCAGTACAGTTACTGTACGCCTTCCTACATACCATTTTCCGACA
GATCAGGGTTTTACTGGAACGGGATTGCGGTTTTCCCTGACCCCCCTAAAGATGGCATGTACCCAAATATGAGTTTGCCTGTGACAGCCGACACAATTCA
CCCATATGCAGAAACCATGGTGAAGAACATCAAAGAGAGAGCGGCTTGGTTTAGAAGCAGTGACGTCTTGTGGCCGTGGGGATGCGACAAGCAGTTCTTC
AATGCGTCCGTTCAGTTCAACAATATGGACCTGTTACTTGACTACATAAACAAACATACAGAATTTGGCGTTACTGTACAGTATTCCACCCTTGGAGATT
ATTTTAAGAACCTATACAATCGCAATTTAACTTGGGGGCTCAGAGGGAGCCAAGATTTCCTTCCGTACTCTTCAGATTCTTCCCAGGCATGGACAGGGTT
CTACACATCCCGCAATGTTCTCAAAGGAGTGGCGCGTAGAGCGAGTTCATTGCTTTATGCAGCAGAGTCCGCTTTTGCGCAGCATCTTCTAAAGCACCCA
ACAGGTTCTGTGGAAAAGGTTTGGGCCATGGAGCAACTGCGCTCCCTCCGATGGGCTGTGTCAGAGGTCCAGCATCACGATGGGATCACAGGCACAGAGT
CCCCAAAAGTCAGAGACATGTATATAGCAAACCTGCTTCAAGGTGCAGCTGGAGTTCGAAAGCTGATGGCTGCAATTGTGATGGATTTGTACAGCTCCCA
AGCAAACAGCTGTGGAAATGGATATATTAACATCACTGTGTACAACCCTTTGGCGTGGGATGTTACGACATTTGTGTTGGTGCCCATGAACAATTCTGTC
TCTGGTGTTTATGATAAGTTTGGGCAGCCCGTGCCAGCACAGATCCAAAATGCCACCGAACCAAGCAATGCCTTTGATTTGCATATACTTGTTACCCTCA
AAGGCCTTAGCTATGGAAGGTACTCTATCAAGCTTGGAAGCTGTGATGCTGCTAATGATCGTCTTGTTACTGGAAGAAAGATAACATTTGTCAGACAACC
AAAAAGAAACAAAGCCAAACGTCAAAACCTGGTTACTCCTATAATGAATGATTGTTACACGGTCGTGATTGATCAGAATACAAACCTGCTGCACAGTATC
ACCGCTAGGGAGAGCAATCAGACTGTCCAGGTAACGCAGGAATTTCTGGAGTACCATTCAAACGGAGACATCAGCAAGGGCCCAATATCAGATAATTACT
TGTTCACCTCCACTGGTCCTGCTATATCGGCCTATAAGGCTGTTGGACTGGAAATTATAGCCGGGAAACTGGTTACTGAAATTAGGCAGTATTTCTACAG
CAATATCACTGCACAGACCTACCTGTACGCAGTCTATTCAAGAATCTACAATGTACCAGAAGGATATGACAAAAGCCTTGTGTGTCACCACATAGAGCAG
AGTTACAGAGTGGGTCCGATGGATCTCAATCGGGAGTCAATTATCAGAACAAGCACAGATCTGCGCACAGACAGATTGATTTACACTGACAACAATGGGT
ATCAGATGCAAAGGAGACAATTCAAGAACTATGACACCAACACAGTGGCACGGAACTACTATCCAATGGTCCGAACTGCCTACATAGAAGACAACAGCAC
TCGGCTTGTTTTTCTCTCTGAAAGGGCACATGGAGTATCCAGCCAGGATAACGGAGAAGTTGAGATCATGCTTCATAGACGTTTGTGGAATAACCTTGAG
TGGGACCTCAATTACAATCTAACATTAAATGATACCTCAGTAGTTAGACCTACCATCTGGCTTATGCTTGGGTCCAGAGCTCTTGCTGGGGCGCTGTATC
AGAGACTTGGAATGGCACTAGAGCACAGGCCGTTGGTCATATACAGCCTGCACACAGGACAAGAAAAAAGGTCATACAAAACTACAGACCTCCCTATCAC
ATTACCTGACAACATTCATCTGCAGATTTTGAGTATTCCTGGTTGGTTTTATTCTTCCCGCCACCATGAGCATATGCAGGATATACATACAGGCAATATT
CGGGACAATGTTGCCGATTTTCACCGTGTTCTCCTAAGATTGCAGCATCTGTATGAAACCAACGAAGACCCCGTCCTTTCCAAGCCTGTAACCATAAACT
TGCAGTCTCTTCTAAAAGGCTTGGGAACAGTGTCTTTAGTAGAGGAACGCTCACTGACAGGAACGTGGGATATCCATACGCTGAAACGATGGAAGTGGAA
AGCAAACATAAAGGGCACGGACCAAAGAGAACACACTCTCGGGACAAGTGACTTCACAATCACGCTCTCGCCGAAGGAAATAAGAACACTATTCGTCTAC
TTTCAGTAAATCCTCCAAAAAGTAGCCTTTCATGGTCAGCCTGTCTGTTCTTTTCACAAACAAAGACCATTGGGTGTGAAATCTGTCATATAAACCTGTA
CAGAGTAGCCATGCAGGGCACTGTGCTGGTTACAGACAGGGGGACATGGGTGCCTTCAATAAAACCACATGCCGCAGGTGTGACATATATTCACAAAATT
CACAACTGCCTGATGCGCATAGAAGGCCACAGTAACTTTCATAATGATTGGCATTAAACATTGAAGTAAATGTAAAGGCTGTACTTTTCTAAAATGCCTT
TTCTATGTGATTTTATAATAATATGTCATATTTGACAGTGCTAAAGATGATTTTTTTTTTTGATAAATGAATTGCAATGGAATATAGAGTTTGAATTGTC
ACTGAAGTGACATTTTGGGCCTGTGGCATGCAAATGTTAATAGATGAATGTATAATTCCCGTAATGGGAGTCTAAATGATTCTGCAACAGAGACGTTGGT
TTGTGTTATGTTATGAATTATTGCACTGTATCCTTGCTGCTTCAGCCAATGTCCCAGGTCCTGCTCTCAGCTTGTACTGGTCAGAGCCTGTTACCTTGAC
CTGACTGTGGGAAAATATGCCCAACTGGGTGGAACAATAATTAATATATATAACTGTATAAAAATGTTGATACCCCTAGAAAATCAGAAATGGAACTTTT
ATTGGAGTGTTTCCTTGGGAAAGGGATGGTGGGAGAGCTTGGGGCAGCCTTACTGGTAATAAAGAAAGCAGTTATAGAATGCGCCACTCATGTGAGTGCT
CCCTCAGTAGTATAAAGGCTTTGGAGTTTTACACAGTGACCCTTTGCTCCATCCATTGCTCCATTTGTACTCTGGCTGCTCAGCTACCCTGCATTGCCCA
ACTTTGGGGGAAATCTCTGCCATTGTAGCACTGGACAGGCTTTCCCAGAGGATTGCAGAAGACCACACAGCACTCTACGGTAAATCTACAAGTTATGGTG
ATATTGGGAGGTGTATGAGTGTCCTCAGACTATACCATCATATCCCTCCTTTTCCCTCATCTGATACTCTCTGAGAATAGACACTAGGGGGTACGTTTAT
AAAAACTGGGCAAATATGCATCTGGGCGGTAACCCATGACAACCAATGAGCAATGAAAGCATACATCTGATTGGTTGTCATGGGTTACCACCAGGTTGCA
AGGTTGCCCATTGTTTATAAACGACCCAGTGGTTGTGGGTACAGCCTCCAAGCCAGGGTTCAACTCAGTCTGGGACCTGACTTTATAAGCAATGTATGTT
TATAACTCCTTCATGCGGGGATATTAAAGGGGTGCTTCACCTGCAAATTAACTTTTAGTATGATGAAGATATTGATATTCTGAGAAAATTTGCAGTTGGT
TTTAATTTTTAGTGGTTTTTCAGTTATTTTGCTTTTTGTTCAGCAACTCTCCAGTTTGGCATTTCAGCAACTATTTGGTTGCTAGGGTCTTATTTACCTA
AGCAACCAGGCAGTTGTTTGACTAAGAGACTGGAATATGAATAGGAGTGAGACTGAGTGGAGGGATGTGGAAAAAAGTGGCAACGGTGAAGGAACAGAGC
AATAGTTGTTTGGCTGCCGAGGTCAGCAAAGAACTGTTGCTCTGGTTTTTTGTTGTTGTTGCTTTTTTCCTTGTCCTTCTGTTCAGTCCCTTTCCTGTTC
GTGTGAAAGCTGGAGACAGACAGGAGAGGAAGGCAAATAATTGATAAATTATAGCAAATAAATAATGAAGACCAATTGCAAAGTTGTTAGGAACAGGACA
TTCTATAACATGCTAAACGTTAGTGTAAAGGTAAACCGTCCCTTTAATAACATTTGTGAGAAAAAAATAAACAAAAGGAATGTTAATATATTTTTAATTT
ACAGTAAACTGTACCAATAAAAAGAATTGTTTAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX843834 [+]aatgctgctggggaactggcagtaagtttgaacatgatcgtcctgcttgtgcttctctctgcctggggatgtgtctgcactcactctgctcaacccatcc
aggcttttgtgattccccacagccacatggatgtgggatgggtgtacacagtacaggaaagcatgcacgcatatgctgcaaatgtctacactacggttgt
ggaagaactgatgagaggggaccgcaggaggtttatagtggtggaacaggagtttttccgcttgtggtggaagttgtttgctggaaatgtagaaaagcag
caggtgcatcagctaatgcgtgaaggaaggctagagtttgttattggaggccaagtaatgcacgatgaagccctcactacgatagatgatcagattttac
agctaacagaaggacatggttttatttatgaaacgtttggagtcaggcctcgtttctcatggcatgtggatccttttggggcctcggctgcgtcccctac
tttgtttgccatggctggatttaatggtcacctcatctcccgaattgactacgacaccaaagccgacatgcaaaatggcaaggagctgcagtttatctgg
agagcctctccatccttatctgacaaacaagagatctttacccatgtaatggatcagtacagttactgtacgccttcctacataccattttccgacagat
cagggttttactggaacgggattgcggttttccctgacccccctaaagatggcatgtacccaaatatgagtttgcctgtgacagccgacacaattcaccc
atatgcagaaaccatggtggagaacatcaa
3' EST: CX843833 [+]ccaaaaactttgcaatggggccccattatttatttgccaaaatttatcaattatttgccttcccccccgggcgggcccccgctttcccacgaacaggaaa
gggactgaacaaaaggacaaggaaaaaagcaaccacaacaaaaaaccagagcaacagttctttggtgacctcgggagccaaaaaactattggtctgttcc
ttccccggtgccacttttttccacatccccccactcagtcccacccctattcaaattccagccccttaggcaaacaactgccgggttgcttaggaaaaaa
aaaccctagcaaccaaatagttgctgaaatgccaaactggagagttgctgaacaaaaagcaaaataactgaaaaacccctaaaaattaaaacccactgca
aattttcccaaaaaatcaaaatcttcctcaaactaaaagttaatttgcgggggaagcccccctttaatatccccccaggaaggagttataaacatacatt
gcttataaag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: