XB-CLONE-3635135
Clone Name: IMAGE:7793327
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Gene:
rprm
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161132 [+]
aaaaaaattcttgtttggagaaaaattgcctgcaaatctttctcttctttgttcctttttcactccttgaagaagcaagaagccaatgtggaaaatctga
ggatttatggatcatttcccctgaactctctttccctactccatcaggagcacctgttcgacttagtaccgcagcctcaagaagtttaggaagatgaacc
agacagaagccggattactgttctccaacagcagtgactctataggaagtatcttggggtgctgcacccaggcttccgttgtaacagacgatggatttga
ggtgccttctcttgacgagaggaacctgtttatcatgcgagtggttcagatcgcggtgatgtgcgttctgtccctcacggtggtgtttgggatcttcttt
ttgggatgcaaccttctcatcaaatccgaagggatgataaactttttggtcaaggacaggagaccttcaaaagaaatcgaagcggtcatcgtcggcccct
attagtgagtaaaaattcactctgatgttgtcaaagaggtgttccgagcaacacagtttgcctttttgtgatgtccgcgtcccatctgtcggagaaatca
aaatcaaaccaaaaaaaaaaaaaaggagagcagatttctcacggacggtgccggaccgagggacaaagatgtgtcgaacgtttattcgtttatttcaatt
ttggctgaaaaatctgggagatcgtctggggggaagtgagaatgaagctgcaactgacatttgtggtggaaatacaatttttagctgcactttaaacaag
aaccacctcacccctccttcatccgaagcagaactagtgagaatgtattctatatcagtatccggcacagacatgtacatcttgcttcatactggacaga
gggattccttacactgattgtaataactttattacccctgcctgactttcctaagagaactttcatacaatgactccgaggcattgttattccaggactg
ttacattttttgtgaaaaattctcactttgctgttgcttttttttaacaaaagagaaaaacttgagaatgtctctttatttctttactgtaaggtgattt
gtatatctttactaatttgtacagttttcggtaacttatatttattccctggcttttttttctgcctcagtaccgactgttttgcttttatccctaaata
ctgaaattggaatgcaaacatgtctatcctatttatatacacatattccccatctatctaagaggcatcgttgatcggttgattcccattatagtgtttt
gtattctttcaataggctttggtgcttccactctttttgaaaatatttctcgaatgatggatacaaagtggacggaagaggttattataatgtccaaacg
gtcacaaaagtgattgcggaggcaatttagccccataatcaaagcatgactagtgcaaattcaggaaatggaattgtttttttttctatttaagatgaat
taaaaacaagaaaaaggctgaattaaaatccaatttttaaatattgtgctcaacttttattctagtcagacactgtttcccaacagttcataatgtctga
atgttttttattttgatagacttattttttttcgggggacggggagctctatgtattacaggtatcagatcccttatccagaaacccattatccagaaag
ttccaaattatggaaaggctatctcccatagactcaattttaatcaaataattcacatgttttcccttttctctgtaataataaaacagtacctgtactt
gatcccaactaagatataattaccccttattgggggcagaacagccctattgggtttatttaatggttaaatgattccctttgctctgtaataataaaac
agtacctgtacttgatcccaactaagatataattaccccttattgggggcagaacagccctattgggtttatttcatggttaaatgattcccttttctct
gtaataataaaacagtacctgtacttgatcccaactaagatataattaccccttattgggggcagaacagtcctattgggtttatttcatggttaaatga
ttcccttttctctgtaataataaaacagtacctgtacttgatgccaactaagatataattaccccttattggggccaaaacaaccctatcaggttcaatt
catgcttgaataattttttttagtaggcttaaggtaggagatccaaattacggaaagatcccttatctggaaaacccaagatcctgagcattctggataa
cgggtcctatacctgtacattaaaactcaatattaggttgacatttttaaattgttcttatttaaccattgtgggcggggcttcaaatattgcattattt
tgtttcaaacatttggttgaaatgtttcttgttcaaaaactgttttccatcagttcatgatgttcaaattgcttattttattgaaactgttttttttttg
gggggggtgccaaatatttcattaaaatcaaattttctatatatatttgttttggaaacactctttcccaatagttcatagtattcctgtttttatatta
ctgtaatgactttttttggggagtggataaaaagattaaaaaaaataataattctgaaaaaaaaaaaaaaa
5' EST: CX846573 [+]aaaaaaattcttgtttggagaaaaattgcctgcaaatctttctcttctttgttcctttttcactccttgaagaagcaagaagccaatgtggaaaatctga
ggatttatggatcatttcccctgaactctctttccctactccatcaggagcacctgttcgacttagtaccgcagcctcaagaagtttaggaagatgaacc
agacagaagccggattactgttctccaacagcagtgactctataggaagtatcttggggtgctgcacccaggcttccgttgtaacagacgatggatttga
ggtgccttctcttgacgagaggaacctgtttatcatgcgagtggttcagatcgcggtgatgtgcgttctgtccctcacggtggtgtttgggatcttcttt
ttgggatgcaaccttctcatcaaatccgaagggatgataaactttttggtcaaggacaggagaccttcaaaagaaatcgaagcggtcatcgtcggcccct
attagtgagtaaaaattcactctgatgttgtcaaagaggtgttccgagcaacacagtttgcctttttgtgatgtccgcgtcccatctgtcggagaaatca
aaatcaaaccaaaaaaaaaaaaaaggagagcagatttctcacggacggtgccggaccgagggacaaagatgtgtcgaacgtttattcgtttatttcaatt
ttggctganaaatctgggagatcgtctggngggaagtgagaatgaagctgcaactgacatttgtggtggaaatacaatttttagctgcactttaaaacag
aaccacctcacccctccctcatccgaagcagaactagtgagaatgtattctatatcagtat
3' EST: CX846572 [+]agttaatataaaaacaggaatactatgaactattgggaaagagtgtttccaaaacaaatatatatagaaaatttgattttaatgaaatatttggcacccc
ccccaaaaaaaaaacagtttcaataaaataagcaatttgaacatcatgaactgatggaaaacagtttttgaacaagaaacatttcaaccaaatgtttgaa
acaaaataatgcaatatttgaagccccgcccacaatggttaaataagaacaatttaaaaatgtcaacctaatattgagttttaatgtacaggtataggac
ccgttatccagaatgctcaggatcttgggttttccagataagggatctttccgtaatttggatctcctaccttaagcctactaaaaaaaattattcaagc
atgaattgaacctgatagggttgttttggccccaataaggggtaattatatcttagttggcatcaagtacaggtactgttttattattacagagaaaagg
gaatcatttaaccatgaaataaacccaataggactgttctgcccccaataaggggtaattatatcttagttgggatcaagtacaggtactgttttattat
tacagagaaaagggaatcatttaaccatgaaataaacccaatagggctgttctgcccccaataaggggtaattatatcttagttgggatcaagtacaggt
actgttttattatta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: