XB-CLONE-3631135
Clone Name: IMAGE:7665425
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Gene:
tmem123
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121264
[+]
GTGCAGAGCGGCAGAAAGTTAGCTGAGTGGGCGCTTTAGGGATTCACTCGGGACTCCTGTCATTACTGTAACTACAATGAGACTACCCGGGGGGAAGGGC
CTGGGGGCAGCGCTCCTTCTCCTGCTCACTGTCTCCTGTGGGGTGCGGAGCGATACCTCGGTTTCAGAACCAAAAGCACCAACAGCAACTGCTCCCGTGG
CATCTACCGTAGGAAGCAATGTCACAAAACCCAATGTAACTGAACCTGCTACCAAGGATAACACCACCACCACCACTGTAGTGCCGACTTCGGCTGTGAG
TAGCACCAACCAATCCACATTGGCTCCATCAACTTCTGCCGCTACAGTGAAAACCACAGCTAACACTTCCTCTTCGGCAGCGACGACAATTGTACCTGTG
AAACCCACAGCGGCCACAACAGCCTCCGCACCCCCTGCAACTGCTGTCGTGACAAGCTCCATTGCTTCCCCGGGGTCGGCTAAGATTTCCGGTTTTGACC
TGGGCAGTTTCATTGGTGGGATCGTGCTTACGCTGGGCATCATAGCCGTCCTCTTCTTCGGCTGCAAGTTTTACAACTCCAGGAGAGGTGTTCGATACAG
GACCATAGATGAACACGAAGCTATCATTTAATATGGAGGGCACCAAAGGATCCCTATTCTCCTCTATCAGCTTTATTCTGTATAATTGCACCGGTTTGGA
ACGGACAGTCGCTGGGTAGAATTCAAGTCTGAGTATCTCGGAAAGATTGTTTGTTTGTAAGCCCACTGGGTACTGATTGGGTCAGTACCATGACGGGAGG
TTACTAGGCATACCTATGAGCCCCTTGTAGCATGGTGTATTCATAGCACACTGAGCTCAGGCTGTCACGCGGACCAGGTCTGTATAATAATGATATGGAA
AATGCTTTTCTTCTGCTTCACAAGAGAAAAATCAATATTTGCACTTTTACCCGTCGCACGATAAGGAAATGAATCTTGCCTTTTGCTTTGCATCCGGTTC
TGTACGGCTGCCTTTTGCCTTATATTTTGTCTGCGTTTTATAATTATCAGTGCGTTTGTCTGTGCCGTGCAGCCATAGGAGGCGTTAGTAACGTATGCAA
GGGTCGGGCATGGGCATTGGGCATCCCTTTTCCCAGGGAGCAGCTGGGGGCATAGATTGGCATTTTGATTAGCAGCCATGCCTCCAGTGGGCAACGGTCA
TCAAAAACGTTGTTTTGCTTATGGAGCCTATGCTGGCATGTAATTGTGTTTATTGGCCTGCTATTAAATACGCTCCCCACATATGGCACATATGGCTAGT
AAAAAAATCTGCACGGCATTTTCATGATTTATGTTATTTGGCTGAAGATCATATTCCTTTTTGTGTAGCAACTAAACTGCATTATCCTTACATTTGTAGC
TTGTCATTCTACACTGCTCCTCTAGGTGTCGCTGTTAGCTTTTTCTTTTTTTTTTTATTCTCTTTACTGTGGCTGAATCCACTACTTTAACAAGAGGTGG
GTAAATTGAAGCCCAATAAGCTTGGAAAACACCAACAGTGCCTTATAACATTGACTTCAAGTGTATCCACCAATCATTGAGCAGCTGGGCTGCAATCTGC
CCAATGTGCGCAGTAATGGGTAGATACCATTAAAGTCAGTGGCGTATGGGACCGGTGCTGTTGGGCAGCCTTTATCACATGACCGCCACAGAGATGTCTC
CATAGGCCCCCAGCTAAGGCCAAATCATTCTTCCGATCCGAGGCGGGGATTGATCTGTCAGCGGATTTCTGTACATTTTGTATAAACCCGGGTAAAAAGA
TTCTTTTTCCCTTTTCAATTGAGGAGATTGATACAGAGTTATTGTTTTTGGAGTGGAAAATAGTTTGTATTTTTTAAATCAGTGTGTAAAATGGTTTTTA
TTGTGTTTTTTTCCTCCATTGTACAGTATGGGATGGATTGAATTTGTATCACAAAGGAATGGGAAACACAGGATTAACTGCTCAGATATATATGGCCTTA
AGCAGTTTATATTTTATATGTGTCTTTGGCTTTAAGGAATGTGTGAATACAATGATCCTGAATTTCTGTTTAATTAAAAGGATAATTTTCAAAAAAAAAA
AAAAAAAAAAAAAAAAA
5' EST: CX850739
[+]tgcagagcggcagaaagttagctgagtgggcgctttagggattcactcgggactcctgtcattactgtaactacaatgagactacccggggggaagggcc
tgggggcagcgctccttctcctgctcactgtctcctgtggggtgcggagcgatacctcggtttcagaaccaaaagcaccaacagcaactgctcccgtggc
atctaccgtaggaagcaatgtcacaaaacccaatgtaactgaacctgctaccaaggataacaccaccaccaccactgtagtgccgacttcggctgtgagt
agcaccaaccaatccacattggctccatcaacttctgccgctacagtgaaaaccacagctaacacttcctcttcggcagcgacgacaattgtacctgtga
aacccacagcggccacaacagcctccgcaccccctgcaactgctgtcgtgacaagctccattgcttccccggggtcggctaagatttccggttttgacct
gggcagtttcattggtgggatcgtgcttacgctgggcatcatagccgtcctcttcttcggctgcaagttttacaactccaggagaggtgttcgatacagg
accatagatgaacacgaagctatcatttaatatggagggcaccaaaggatccctattctcctctatcagctttattctgtataattgcaccggtttggaa
cggacagtcgctgggtagaattcaagtctgagtatctcggaaagattgtttgtttgtaagcccactgggtactgattgngtcagtaccatgacgggaggt
tactaggcatacctatgagccccttgtagcatggtgtattcatagcacactgagctcaggctgtca
3' EST: CX850738
[+]gaaaattatcccttttaattaaacagaaattcaggatcattgtattcacacattccttaaagccaaagacacatataaaatataaactgcttaaggccat
atatatctgagcagttaatcctgtgtttcccattcctttgtgatacaaattcaatccatcccatactgtacaatggaggaaaaaaacacaataaaaacca
ttttacacactgatttaaaaaatacaaactattttccactccaaaaacaataactctgtatcaatctcctcaattgaaaagggaaaaagaatctttttac
ccgggtttatacaaaatgtacagaaatccgctgacagatcaatccccgcctcggatcggaagaatgatttggccttagctgggggcctatggagacatct
ctgtggcggtcatgtgataaaggctgcccaacagcaccggtcccatacgccactgactttaatggtatctacccattactgcgcacattgggcagattgc
agcccagctgctcaatgattggtggatacacttgaagtcaatgttataaggcactgttggtgttttccaagcttattgggcttcaatttacccacctctt
gttaaagtagtggattcagccacagtaaagagaataaaaaaaaaaagaaaaagctaacagcgacacctagaggagcagtgtagaatgacaagctacaaat
gtaaggataatgcagtttagttgctacacaaaaaggaatatgatcttcagccaaataacataaatcatggaaatgccgtgcagatttttttactagccat
atgtgccatat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: