XB-CLONE-3614273
Clone Name: IMAGE:7675167
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Gene:
rnf31
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127272 [+]
ACCAATTAAAAGCCTAGGACATTCTATATTAGATGAATTCCCCCTTTAAAATAAATACAGGTATGGAACCTGTTATCCAGAATGCTTGGGACCTGGGGTT
TTCCAGAAATGGAAGAACATCCTATAAGGAGTGCTCTGGAAAATGCATTAATTAATCAACCGGGGGCTGTGACTCCTGAATTCCTGAATAAAATGTTGTA
TTTGCCTTTGAGAGAACGGTATAGAAGACTAAGTGTGGAGGATCTACTCAAAGGAGGGACACAGGGTGGACGATTTGTTCTGTCTCTTTATGGCTACTCT
CAGTCACTTCCTGATGGTCTAAGTTTTCCAGATTCTGTGCAGGAACCAGATGTCTCCACTGTTGCTGCTGTAACAGCTGATGTGATTATTCTTCACCATG
AACTCAACCTACTCATCTCGAACATGCACCCACGGCACGAGGAAGCAGCACAAGAACTATTAGGTGGTGAACTGCCACAGCAGTCTCTTGTTGCTGATTA
CGTAAGTGCTCCTAAAGATCCACAACCAGAAACCACTTCTGCTGGCTGTTTTCTTTGTGGTACTCTAGTTTGTTCTGTGTTTTGTTCCTCTTGTAATGAG
ATGCTCTGTGAGGAGTGTGATAAGAGAGCACACTCTCATCCAGTCCGTGCCCAACATGTGCGTTTACCATGTGGAAATACAGCAAAGCCCAGGTCATTGG
ATCAGGACTCCCCTTCTACAGTGTCATCTCGCCCTCCTTGGAGATGTTACTCTTGCCATTTTGAAAACAAAGGGGGTTCTGTTCTGTGTGTGGTATGTGA
GCGCCCCCGTGGCTGTACCAGCAGCCTTTCAAACCTATCACTTGGAAGTACTTTACAGAGAGACAGCTGGGAATGCCAGGCTTGTACTCTGCAAAATTCT
GCATCTGCAGTTCTGTGTGCAGTGTGTGATCGTCCCCGATTGGCAAGCAAACCAGCCCAGGATGGTTACGATTCTGTTGCTTTGCTTTTGAGAGATATAC
AGAGGGATAACAAGAGTATCACATGCCCTGTGAGTGAAAAAAATGGTCTATTTGCCATGAAGAATCATCAGGAGAGTATGCATGAACCAGAAAGCATTGG
TCCTCTGATAGATCATACAAGGCAAGAAGAGGAGATTGCTAAAAGTGAGGGCTGGCAGTGTTCCCATTGCACTTTCTTTAACACACGAAATGGACGTGTT
TGTGAGATTTGTGACAGGACCAGTGAGGGGACAGCAGCAACTGAACAACCCAAGGCACTTGATATGGCACATGTGCAGGAGGAGATCCGGCAGAAGCGAC
TAAGAGAGGATGGAGCTAAAATAGTGGCATTGATCCGGGAAGGAGAGAAGTATGGGGTTCTACCTGAAGAGGTGTGTAGCGCAATACGATATTCTGGTAC
AGAAGTGCCTCAGTGGTGGCTGCAGACTGAACTTCCCTATGTATTAGAGCGTCTGCTGGATGCTGCTTCACAGAAAGCAGGAGATACAATTGGTGCCCTA
ACAGTGGAAGAAGCTCGTGATGCTTGGGTGTCCAGTGGTGGAGACATGGATGCTGCTGTCATCTTATGTGTGACAGAGCGAAGAAGGAAGGTTGATGCAC
TCTCCGGCCTAGGTTTTCCAGAAAGGCATAAAGTGGTAGCTGCTCTTTATGAAAGTGCTGGAGATGTTGGTAGAGCACTAAGTATATTACAGAGACCACT
TCTGGAGCCTTTTCTTATACGCATGTGGGAAGAACCACAACCACCCCTTCGGCTAGACTCGGCAGATAGACAGGCAGTTCTCCGTAGGTTACTTGCAGAA
CATTCCTTGCACAGTTGGGGTCGGGCAGAGCTAGCTTTGTCATTGCTTATTGAAGGAGAAGGGCGCTATGAACTCCAGGATGTTGTAGAGGCAGTGCGGG
AATCGCAGGACAGAGACTTTATCAAAAGGATGTTAACACAGGAGTGTGCAGTGTGTGGATGGGAGCTCCCCCGTAACAAGATGCGTTCTCTAACCTCATG
TGAGTGTTGTATCTGTCCTGATTGCTTCCGGATGCATTTCACTGTAGCAGTGAAAGAGAAACATATTAGAGACATGATATGTCCAGCTTGTGAGGAGCCA
GAAATAAGCGACGAGGGGGAGCTTCTTCACTATTTCTCTACTCTGGATATCCTGTTACGGGACACTTTGGAGGTTGATGCATATGATTTGTTCCATAAAA
AACTAACTGAAAGAACACTTATGAAGGACCCAAAGTTCCTGTGGTGTACACATTGTTCCTTTGGATTCATTTATGAACGAGATCAGCTGGATGTAAAATG
CCCCCAGTGTCAGTGCAGTTTCTGCAGGAAATGCAAGAGGCCTTGGGAAGAACAGCATCGGTCTTTAAATTGTGATGATTTTCAGAACTGGAAAAGGGAA
AATGATGCAGAATACCAAGCTCAAGGATTGGCTGTATACCTGCTAGAGAATGGGATCACCTGTCCCCATTGTAAATTTTCTTATGCTCTTGCACGAGGGG
GATGCATGCACTTCATATGTTCTCAGTGTCGCCACCAGTTCTGCAGTGGCTGCTATAACACCTTTCACCAAAGAAATAAATGTACAGTCCCGTCCTGTAC
GGTAAGGATGTCTCTCCATGCTCACCATCCTAGAGACTGTCTCTTTTACCTCCGAGATTGGGATGTCACACGTCTGCAAAGACTACTCCAGATGTTACTG
GTACAGTTTAACACAGATCCACCCGCTGGAACACAAACTGCTCCAGGAGGTGGGTGTCGTGTGATGGAGCAAAAGGAGATACCTGATGGCCTGAGAGACG
AAGCTTGTGGCAAAATAACACCTCCAGGATATGCTGGTTTGTGCGAGTCTCATTATAAAGAATATCTTGTTAGTCGTATTAATGCCCATTCTCTGGATCC
ATGTGTTCTCTATGATCTGGCTGAGACTATGAATGTGTGTCAGCGCTACCTGCCTGTATTGCCTCTACAAGATCCCACCGAGGATGAGGCCAGCTACAGG
GAGCGTCTCCTGGGGGTGTTAATATCAGAGGTGCCACTGAGTATGAACATTCCACGGAAGCAGAATTAATACTGGAATTCCATTTGCAAAGATCTCTGGT
AGTGCTATGTAAAGTAGCACAGGTTAGCTACAGTATCTGAATCAACTGTTGTACAAAAAAAAAAAAAAAAA
5' EST: CX886395 [+]accaattaaaagcctaggacattctatattagatgaattccccctttaaaataaatacaggtatggaacctgttatccagaatgcttgggacctggggtt
ttccagaaatggaagaacatcctataaggagtgctctggaaaatgcattaattaatcaaccgggggctgtgactcctgaattcctgaataaaatgttgta
tttgcctttgagagaacggtatagaagactaagtgtggaggatctactcaaaggagggacacagggtggacgatttgttctgtctctttatggctactct
cagtcacttcctgatggtctaagttttccagattctgtgcaggaaccagatgtctccactgttgctgctgtaacagctgatgtgattattcttcaccatg
aactcaacctactcatctcgaacatgcacccacggcacgaggaagcagcacaagaactattaggtggtgaactgccacagcagtctcttgttgctgatta
cgtaagtgctcctaaagatccacaaccagaaaccacttctgctggctgttttctttgtggtactctagtttgttctgtgttttgttcctcttgtaatgag
atgctctgtgaggagtgtgataagagagcacactctcatccagtccgtgcccaacatgtgcgtttaccatgtggaaatacagcaaagcccaggtcattgg
atcaggactccccttctacagtgtcatctcgccctccttggagatgttactcttgccattttgaaacaaagggggttctgttctgtgtgtggtatgtgag
cgcccccgtggctgta
3' EST: CX886394 [+]gtacaacacgttgattcagatactgtagctaacctgtgctactttacatagcactaccagagatctttgcaaatggaattccagtattaattctgcttcc
gtggaatgttcatactcagtggcacctctgatattaacacccccaggagacgctccctgtagctggcctcatcctcggtgggatcttgtagaggcaatac
aggcaggtagcgctgacacacattcatagtctcagccagatcatagagaacacatggatccagagaatgggcattaatacgactaacaagatattcttta
taatgagactcgcacaaaccagcatatcctggaggtgttattttgccacaagcttcgtctctcaggccatcaggtatctccttttgctccatcacacgac
acccacctcctggagcagtttgtgttccagcgggtggatctgtgttaaactgtaccagtaacatctggagtagtctttgcagacgtgtgacatcccaatc
tcggaggtaaaagagacagtctctaggatggtgagcatggagagacatccttaccgtacaggacgggactgtacatttatttctttggtgaaaggtgtta
tagcagccactgcagaactggtggcgacactgagaacatatgaagtgcatgcatccccctcgtgcaagagcataagaaaatttacaatggggacaggtga
tcccattctctagcaggtatacagccaatccttgagcttggtattctgcatcattttcccttttcccgttctgaaaatcatcacaatttaaagaccgatg
ctgttcttcc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: