XB-CLONE-3613929
Clone Name: IMAGE:7674980
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Gene:
ano1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167593 [+]
ggcgtgtctccaggtgtaatctgtgcggaacgtttccccgcctctgagtaaggccaggcggatagggtgattgggccccgaaggtaccattggtggtgcg
cacagtatatagaatatagccggactgctgggcattcttacaccgcgggaaatggccgccagagacgaccagcgcgctgcctgcggaggcttccagcatt
cttctgagcccagagcaacttccaatgaaacaatgagggaaagtcagaaggagtatgcggacgataatgacaacggtatccacattatcaatatcaaggc
tattgaagacattggatatgtccagtctgatggcatgttactaaaatccttgggaacagatcaggatagcgcctgtaaacatggcttatactttagagat
gggcagaagaaggtggattatatcctggcatatacctacaagaaatcctccggcagcagaacaccaaacaaaaaaaaccatcacagcaactccgtacata
gcttgcacagcacaaaggatgatcaccatctccccgggggagcagccgacctggaggcaatggaagcagagcctaaaatggattatcatgaagacgacaa
gcgtttccgcagggaagagtatgaaggcaacctgatcgagactggtctagaactggaaagagatgaagagactaaacagcacggcgttggctttgtaaag
atccacgctccttggaatgttctgtgcagagaagctgagtttatgaaactcaagatgcccacaaaaaagttctatcaaatcaaccagactccaggcctga
tgcagaaactgagctcttttattcagaaaataagcgaaccaattcagcccaaggttgctgggctccgaccccagacagtaaagcctctctcctacccctt
ctccagggagaagcagcacttattcgatatgtcagacaaggactcgttttttgacagcaaaacaagaagttccattgtgtatgaaatcctcaagcgaaca
acctgcaccaaagctaaatacagcatgggaattaccagcttgcttgccaatggtgtgtacagcgctgcctatccactgcatgacggtgactatgaaagtg
aagccgaagccaatgataggaagctgctgtgtgacgagtgggcaagttacagcgttttttacaagtaccaaccaattgaccttgtcaggaaatattttgg
ggagaagataggcttgtactttgcctggctgggagtttatactcagatgcttattccagcttcaattgtgggaataatagtgttcctgtatggatgtgcc
accgttgatgaggatattccaagcatggagatgtgtgacaatagatacaacattaccatgtgccccctgtgcgacaggacttgtaactactggaagatga
gctctgcgtgtggtacagccagggccagccatctctttgacaatccggctacagtattcttctcagtcttcatggctttatgggctgccagcttcatgga
gcactggaagagaaggcagatgcgcctcaactaccgatgggacctaactgggtttgaagaggaagaggatcatccaagggcagaatatgaggctaaagtt
ctgcagaaatctctgaaaaaagaatacaggcacaaagagactgaaaaggagaagctcacctggagagacaggtttccagcttacctaacaaacgtggtgg
gcatcatttttatgattggcgtgacgtttgcaattgtgttcggagtcatcatgtaccgaatatccactgctgcggccttagcgatgagctcaaacccaaa
cacccgtgcgaacgtgagggtcactgtcacagcgacggcagtgatcatcaacttggtggtgattctgatccttgatgaagtgtatggggcaattgcccgg
tggcttacgcagattgaggtccccaaaactgaccagacatttgaagaacgcttgatattcaaggctttcctgctgaagtttgtgaacgcttacacgccca
ttttttacgtggctttctttaagggcaggattgttggacggcctggagattactactatatatttcagtcatttagaatggaggagtgtgcccctggtgg
ttgtttgatggaactgtgcatccagctgagtatcattatgttggggaagcagctgattcagaacaatctcttcgagattggaatcccgaagatgaagaag
ttgatcagataccttaagtcaaaaagacagtcctataaggaccacgaggagcacttaaagaagaagcaacgttatgaggaggattatgacctagaaccct
ttgcaggtctgactccagagtacatggaaatgataatacagtttggttttgtgaccctgtttgttgcctcttttccactggctcccctctttgctttact
gaacaacatcattgaaatccggctagatgcaaaaaaattcatcactgagctcaggagacctgtagccgtcagagcaaaggatataggtatctggtataac
atcattcggggactcggcaagcttgctgtgataatcaatgcctttgtgatatctttcacatccgacttcataccacggcttgtttacctctacatgtaca
gccctgatggcacaatgcatggctttgtcaaccacacactctcctactttaatgtcactgactttaaaccagggactgaacccagcgagcctgtagatct
tggatacaaagttgaattgtgcaggtacaaagattacagagaggcaccatggtctccgcataaatacgatattaacaaagactattgggctgtgctggca
gcacgactggcttttgttatagttttccagaacttggttatgttcatgagtgattttgtagactggataattcccgatattcccaaagatatcagcgaac
agatccacagggagaaagtcctcatggttgaggtgttccttaaagaagagcagggaaaactgcaaatgctggaaacgtggaaggagcacgaacggagaaa
aggagaaaactgcaacagccaaagcccaggactttcccgcagtcagagaggaagttcgtcgtctatgtattctcatcgtgtggatgtttagagagcgcgc
agggcagtttaaggcattcattccactgatagaaattatcagcagcttgacccacccaagagcccatgagttcatctttcaagtttattaccttttaccc
ttggtggaatttgcaggaccatgtgtttacatgagcagcaactgtttagaacatgtatctgcatggggggaaaattagcaatagtataggttaatttaat
ctctattcggttttctttttgtttgtgccttgtgctggagatcctgcgaaactctttaagctttactcgtagaattaacccctgtgactgctggaggtcc
attgaaatattttgcacagagttcggcccgagacaagcccccagttgcgtggatatctttactggacatgcctgtgctgacattccacagtggacaatgt
gtttaagtgcaggcaccctaaactgattcttgggatgaccctctcacgagccaaacacacattggaaggcggatgtattggttatctgttgcaccacgta
cctctttggtcatgaagaatttttactgtactgaacagttcagtttaacgcagctcgctaagccgatgctgtccgttgcagcgctaacaggttacgcctt
cagagagaatatttttatagaaactacctatgcaatgtaaagattttcctttctttatataaatcttttatacctatttattgataacaaagtttattag
gaagcggtatagtcctgcttccatttagtcatttcatgccggaaaaaagaaaaaaaatctgcactggccttcagtacaagaaaaccctctgtggttaatg
aaattcaattgccttgtctgttctgttttattgtttttgttttttttgggaattttcatagtttgacaaaaactgttttaacttgtttatgcaacctaaa
ctgttacctgcaatgtgtaatgtgtttttgtctgcaggcgtcggctgccttttaggactggcaatgttccataacataaagcctgagctagccgtcgttc
tacgcactgaccaaccaaagacatgaaaagggctataatagcttcagcacctcaagaacgtttcataccgtcctaaagtcggaatagttgtctaagtgac
ggaagtcacgattttacggttataagtgcctgaccatattagttggctgcggcagatattctgccgggttacttaatcagcattatcttcactattatta
gactgattcaaaatgacaagtgttgtgtgagggtgtggggttaaacatgcagtaaaatccaagttggtgtttaatttctttatttgcaaatgaaaaaaat
gtatattttaaacaagggtggtggtgagcaacccagagcagcctgctgattggtcagggctttctatccacaaacccacagaattaacaagaggtaagtg
tagagcctgtgttcttaatgggactcgacccttagtcgctactctaaaattccagtcctgttgtttcagaaacaccggtctccatttcaaagcaaaaatc
tcacatatatttatttcaaaaaaggataattttacacacacaaaaaattgtcttccaagattgactaaccacagtctggagctttcagcatgtacatgtg
taaaagctacaggcccttctatctagaacttcatacgattgttagagttcaggtttacatttcaccttgacaataagtagcctgatgactttcctgtcac
tcaaatacattttccatgtacagcttctattggttggcaacatagcatggaataagaacgaaggtaggaaacggagatgatctttatacgttatggcaca
tttacatgattattacaaattcctttcttgggttttttttttgttttttaagttaaaatcttgattttggtgaaaattatataaataaaatctaatatgc
agttaaaaaaaaaaaaaaaaa
5' EST: CX886761 [+]gcgtgtctccaggtgtaatctgtgcggaacgtttccccgcctctgagtaaggccaggcggatagggtgattgggccccgaaggtaccattggtggtgcgc
acagtatatagaatatagccggactgctgggcattcttacaccgcgggaaatggccgccagagacgaccagcgcgctgcctgcggaggcttccagcattc
ttctgagcccagagcaacttccaatgaaacaatgagggaaagtcagaaggagtatgcggacgataatgacaacggtatccacattatcaatatcaaggct
attgaagacattggatatgtccagtctgatggcatgttactaaaatccttgggaacagatcaggatagcgcctgtaaacatggcttatactttagagatg
ggcagaagaaggtggattatatcctggcatatacctacaagaaatcctccggcagcagaacaccaaacaaaaaaaaccatcacagcaactccgtacatag
cttgcacagcac
3' EST: CX886760 [+]aactgcatattagattttatttatataattttcaccaaaatcaagattttaacttaaaaaacaaaaaaaaaacccaagaaaggaatttgtaataatcatg
taaatgtgccataacgtataaagatcatctccgtttcctaccttcgttcttattccatgctatgttgccaaccaatagaagctgtacatggaaaatgtat
ttgagtgacaggaaagtcatcaggctactta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: