XB-CLONE-3613000
Clone Name: IMAGE:7676025
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Gene:
nhsl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX887710 [+]
ttagaagaatcagagcacagaaagggcaaggaattgcaattctgatgtccacttcctctggcaacatgtctactctaagtgactctgcagcttgttcttc
atatctaaatggtaacctgcacttccgtagtctgcccaggattggtgcaagagaatctcttcaatcccttaaccaaaaaaattattattacagcaacatg
gaggacccttttacaactttgccttggcagttaaacaaactgcaagtggatgatactaatgttcaattaaggagtaaccctaggtcaactattaatcaaa
ggcctgagtctcaagaagtaaaaaattctactagtgaaattgcctcaagtccagcatgtatggtctcgcctcattcttcttattcaacaaacattattcc
aaatgccatactttcatattcctcagaagtaataagtattcacactccccacagaggtggacttctggacaagaaaccaagagtccacagttcatcttct
aacagaaatggtattagcagggactatacttctggcattaaagataattcctcacctggtaactggcgtgaaaacagttcatctaggaactcacagatat
cagatacaaagtcacctatcagtaaaaaaagagatgttccacacaacgttccttgcaaaattaacactggctttaacaactactcatttgacagtgacaa
tcaaagtgaaattagctactcagatggtcgtccaagaaatggtagtataactagcagcattaatagtgctgacaactggttgtatgaatctaggcacaat
gac
3' EST: CX887709 [+]gtattttcagccttgctttatttgaagcataatcacagaattattatagtactaataaaataaaggcccctgaatgtgtacagtaatagaaaggacagag
tcacagccacatttaacaaacgtacataaataaattgcaggtctgcattgcatatttatggctgtcaaaaactccagcaataaatgagttaaaaagagtt
ttagtagagtaaaaaatgtacaaactttgaatgcaagtaaaaaggtgagatttgcaatgtcagtatatacaaatattataaagataacagtacattaaat
gatgcattcagttaataaaagggacatggaatgctatttgaactttaagaagtaaatatttttacattaatattgcttaaaaggtagaaaagctgttttc
tttggtaatctcaaattttgcatttcttttttagatgataaaaacaatgggcttatttagcatgacaactagtacacattcttttgcagtcaagtgatct
aattgccaaacaatgcaagagttacaaagacggctcatagctcaagctgaagaaagacaaacgtgtatccagcagctttcaaggcaacaatacatttcaa
agcaatatgaaatactgcaaaacaatgtattgaaggcacttatactagtaacacagcctaaagttccctttagtagaaacgtacacactgcaatggttgg
attgtatgcagcactcaaattgcagctcactagaaaacttcattaaatcttaggta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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