XB-CLONE-3608960
Clone Name: IMAGE:7677995
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Gene:
upf2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168584 [+]
aagatcgggttaaggaagaaccaaaagcaagcaccaaaagagatcaatcaaaggcttctgaggaaaaaaagaagcgaactgatgaggacaagcggaaaaa
agaggagaaggagtgcaaaagaaaggaggatgatcaagtcaaagcagatgatgagcagcaaagttcagagggaaaaaatcaagaagatgaaataaaaaag
aagcaggaagaagaggaagctcatcagagagaagctgcagcattacacatcaaagagaaggatgaagcaaatcagttacaccaagaagcatgggaacggc
accacactaggaaggagcttcgtagcaaaaatcaaaatgcccctgaaaatcgacctgaggagagtttctttagcaggctggactctagtttgaagaagaa
cactgcttttgtgaagaaactcaaaaccattactgaacagcaaagagattcattgtcccatgacttcaacagccttaacctcagcaagtacatagcagag
gcagcagcctccattgtagaggccaagttaaaaatatctgatgttaactgtgctgtgcatctgtgttctcttttccaccagcgatatgctgactttgcac
agtctctccttcaggtatggaagaagcattttgaagcccgcaaagaggaaaagactcctaatattactaagctgagaacagacttgcgctttattgcaga
gctaaccatcattggcatctttactgataaagaaggcctctctctaatttatgagcaacttaaaaacataattaatgctgatcgagaatcccacacacat
gtttcagtagtaatcagtttttgccggcactgtggagatgatattgcaggcttagtgcccaggaaagtgaagtgtgcagctgaaaaatacaatcttaatt
ttcctcctagtgagattatcagcccagagaaacaacagcctttccagaatttgctgaaagagtatttcacatctctgacgaaacacttgaagagggacca
cagagaattacaaaatatagagcgccagaacaggcgcattcttcacactaaaggggagctaagtgaagacaggcacaagcaatatgaggagtttgcaaca
tcttatcagaagctgttagctagctgtcaatcacttgcagatctgctagatgaaaatatgccagaacttcctcaggacaaacctgctccggaggaacatg
ggcctggtattgatatcttcacacctggaaaacctggagattatgacttagaaggaggcatttgggaagacgaggatgcccgtaacttttatgagaatct
gattgatttgaaagcttttgtcccagccattttatttaaggataatgagaaaaattctcagagtaaagaaactggcaaagacagtttcaaagatagtaaa
gagaaagatgtgtccacaactgaagagctagagctggagttagagacattggatattaatgatgaaggcatggaactggaaggtgccgaagatgcagaag
acctaacaaaaaaacttttggatgaacaagagcaagaagatgaagaggcaagcacaggctcccatttaaaactcattgttgatgcttttattcagcagct
gccaaactgtgttaatcgtgatctaatagataaggctgcaatggatttttgcatgaacatgaatacaaaagctaatagaaggaaattggtcagagcacta
ttcattgttcccagacaaagactggatcttcttccattctatgccagattagtggccaccttgcatccttgcatgtctgatgttgcagaagacctttgtt
ccatgttaaagggagactttcgtttccacgttagaaagaaggatcaaatcaacattgaaacaaagaacaagactgtgagatttattggagaacttgccaa
atttaaaatgttcagcaaaacagatacgctgcattgtttaaagatgctgttgtcagacttttcgcatcaccacatagaaatggcatgcactctactggaa
acttgtggcagatttctttttcgatctccagattctcacttgcgcaccagtgtcctgctggagcaaatgatgcgcaagaagcaagcaatgcacttagatg
cacgctatgttacaatggttgaaaatgcctactattactgtaacccacctccagctgaaaaaactgtgaaaaagaagcgacctccactacaagaatacat
cagaaagcttctttataaagacctttccaaggtcaccactgaaaaggttttgaggcagatgaggaagctgtcttggcaggatcctgaagtgaaggactat
gtaatttgctgcatgatcaacatctggaatgttaaatacaacagcattcattgtgtagccaatcttttggcaggactggtactttaccaagaggatgttg
gaatacatgttgtagatggagtgttagaagatatacgtcttgggatggaggttaaccagccaaaatttaatcagcggaggataagcagtgcaaagttcct
tggggagctttacaactatagaatggtggaatctgcagttatatttcgcacactttattcatttatttcctttggtgtaaattctgatggaagccccagc
ccacttgatcctcctgaacacttgttccgcattcgattagtttgcactatccttgacacttgtggccagtattttgaccgtggatcaagcaagagaaagc
ttgattgttttcttgtttattttcagcgctacgtttggtggaagaaaagtcttgatgtgtggactaaagaccacccatttccaatagatattgattatat
gataagtgataccttggagcttctgaggccaaaaatgaagctctgtgcctcactgaatgaatctgtccgccaagtgcaggatttggagcgtgagtttctg
acaaagctaggtctcatgaatgataaggaatccaaacattccatgacagaaggagatcacctagtgtatgaagatgatgatgaggaagaagaggctgggg
cagagacagaggagcagtctggcaatgaaagtgaaatgaatgaacaagaagaagatgcttctgaaaatgaagaggatgaaagagaggaggaagaagagga
gaatacagattatatgactgactcaaacaaagaaaatgaaacagatgaagaaaacactgaggtgatgattaaaggaggtggaattaaacatgtaccctgt
gctgaagatgaagactttattcaagcccttgacaagatgatgctggaaaatcttcagcaacgtagtggagagtcagtgaaggtgcatcagttagatgttg
ctattcccctgcatcttaagagccagcttaaaaagggaccagctatgagcactggagaagtagacccggaatctggcgacactatgccatttgttatgct
tacaagaagaggtaacaaacagcagttcaagatccttaatgtgcctatgtcctcccaactggctgcaaatcactggagtcagcagcaagctgaacaagaa
gagaggatgaggatgaagaaactcacattagatatcaatgagcgtcaggaacaagaagattaccaagaaatgttgcagtcattggctcagcgcccagcac
ctgccaacacaaacagggagcgcaggcctcgataccagcatccaaagggagcacctaacgcagacctcatcttcaaaactgggggcaggagacgttgatc
cagcacagtcattccattgggccctgtgtcttcaacatggctagtgtaaacctccagaaattgtaaaagatcagcagcacatcgcagcatgttcagaaca
gaaggttgggatggaatcctgtgacagggcttgggagcaaggcgaagacagcaataccagcttctgcttcagacagaaagaagcttcaagcgtaggcacc
actgacacacaaacagtggggccctttttgtatctgcaaaaacgctcaagacagccttacattggatggaaagcattaagagggtttactttattagcag
gcagtttggctaaaagtagacagaagtttcctttaagatggacatgcattgcaactgtattgtagttttgcactgttagaaggatccagcgaaaagatgt
aggactggacgtcacactttgaaagaacacagagttgcaaaacaatagccaatatggcacaaaggggcaaaaagctggaaacgttgaacacaggaagtca
gacaaatctggagtgcaacaaatactcctaagaagattttttgtttaaaatgtgtttgctttttctctgtaatagtacagtgtaaaatggaaagacactg
tgccttttggcaaatgccagagcagactgggcttctttggctgtgcatagaatttgccaaaatttgtatagagctaaaatgtgcagtttgcatttctctt
ccattcctataccagctgaacacaagaactggccagactaccaacgtatgctctattttggcttctcaacatttagcctgttgcaggattacagctaagt
actagagtaaggagtgaagcagttagtgatgttacctttaaaggctagctctttggtatttacaagtttaatttgataagtaacagatttatttttccca
atttttccatctgccaagtaaataaaacctttttctgtttgaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
5' EST: CX891775 [+]aagatcgggttcaggaagaaccaaaagcaagcaccaaaagagatcaatcaaaggcttctgaggaaaaaaagaagcgaactgatgaggacaagcggaaaaa
agaggagaaggagtgcaaaagaaaggaggatgatcaagtcaaagcagatgatgagcagcaaagttcagagggaaaaaatcaagaagatgaaataaaaaag
aagcaggaagaagaggaagctcatcagagagaagctgcagcattacacatcaaagagaaggatgaagcaaatcagttacaccaagaagcatgggaacggc
accacactaggaaggagcttcgtagcaaaaatcaaaatgcccctgaaaatcgacctgaggagagtttctttagcaggctggactctagtttgaagaagaa
cactgcttttgtgaagaaactcaaaaccattactgaacagcaaagagattcattgtcccatgacttcaacagccttaacctcagcaagtacatagcagag
gcagcagcctccattgtagaggccaagttaaaaatatctgatgttaactgtgctgtgcatctgtgttctcttttccaccagcgatatgctgactttgcac
agtctctccttcaggtatggaagaagcattttgaagcccgcaaagaggaaaagactcctaatattactaagctgagaacagacttgcgctttattgcaga
gctaaccatcattggcatctttactgataaagaaggcctctctctaatttatgagcaacttaaaaacataattaatgctgatcgagaatcccacacacat
gtttcagtagtaatcagtttttgccggcactgtggagatgatattgcaggcttagtgccccaggaagtgaagtgtgcagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: