XB-CLONE-3605931
Clone Name: IMAGE:7679630
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Gene:
cep78
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC131834 [+]
TGGGGAGGCAAAACATTTTATTGTTAGGGCCCCATGTTTCCTGATGGCGGCCCTGACTTTGCGAAAGTAACTTATAACTTGTAAGAGACAAGCCGACATA
ACGAGTAAGCACAGGAGAACATCAAATCACTGATATGAAGCAGCAGTATTCTTGATCCCAGCGGTGGGAAATGAGAGGGGCGGCACTTGGAAAAGGTCGA
GCCGAGGAGACTAAGATGGGTTGGAAGGGAAGGAACCATCTCTGACCGAAGAGGTCAGTAGGAGGGGAGCAGGAAGCCGGGCACTCGGAAAGGGCGGGGA
AATGGAAGGCGGTTAGGAAACCAAGGAAACGGCCCCACGCTCAGCCTGCAGAGTAGGCATAGGGAAGCAGATGGCACAGTACGATTGTTGTTGCGGGAAG
CTGATTACTACGAAAGCTGCATGCTTACTCCCGTGAGTCCGCCAGCACCTAAAAGAATTTGTGTATTCTTGAAAGATGATTGACTCAGTCCGTATACGCC
ATCAGGGTTCCATGGATTTCCTTTCACACTATGAATATCTGTGTGCACTTCAGGATTCTGTTCCAATTCCAGCTGTAAAAGCAAACATCCGACATGGCAG
TCTTAGCTTCAATGCAGATCGGATAAAACTCTCTGACTGGCTGCCTATTCTAAATTCTCTGAAAATAAATAAGAGTTTGCTAAATGTGTCAATAAAAAGT
TGTCACCAGCCTGGACTTGGGGAATTTGGTGCTGAGAAGTATGGAATATATTTCAAAAGGCGCATCCCCCCCATCCGATCAAAGGACATGACTTTTCAAG
TATGCAAAGCAATTACAAGCTGCTTGAGTGTATCAGGCTCTTTAAAAGAGTTAGAGCTCCATGGACTGCCTCTACGAGAGAGAGATTTAAGAATTTTAGC
AAAGGGTTTGGCTGCATCCTCATCTGTGGAAAGCCTGTCTTTGGCATATTGCTCCTGTGGAGATGAAGGACTGGAAATTATTTGTCAAAGTGTAAAGAAT
TCACCTACAATCAAGACAGTAAATTTCACAGGGTGCAATCTGACATGGCGAGGAGCAGAACACATAGCAAGTATCATCAAGCATCAGGCTACCAGAAGGC
ACAGTGAAGCCTGGGCTGAAAGCCTGAGATACAGAAGGCCTGATCTTGACTGCATGGCTGGTTTAAGGCGTGTCACTCTTAACAGCAATACCCTTGTGGG
TGACCGTGGAGCCATTGCCTTGGCAGAAGTGATAGGCGAGGACCTCTGGTTAAAAGCACTAGATTTGCGCCAGTGTGGTATTTCCAATGAAGGTGCACAA
GCATTTCTTAACGCATTTCAAACAAATACAACACTGATAATACTGGACATCAGAAGAAACCCGCTAATAGACCGTTCCTTACTGAAGACCATTATTGAGA
GAGTGTTAATGAATGCACATGATACCAATTCAGAGTACAAATGGTACACATCTCCGTCTTCTAAGGACAATGGGAAAGTAAGGCAAAAATGGAGATCTAT
AAACAACTGGAATGGACGGAAAGGAAAAAATATTGTCCGTGTTGGGTTTTCAACTAAAAAGCCAATGGTCATTGGAAGAAAGTATTCCTCAAAAGAATTG
TACTGTCCCGATCCAAGGCCTCCAGGAGCTGAGGGATTTCTGCCTTGGCGTACAGCAGAAAGAGCAAAAAGACACAGGGACGTTTCAGGAGAATGGGCCT
CTCCAGGAAAGGCAGATACTGCAGTGAAAGTGATTATGGATAGTGAAACCTCTTCAGAAAGTGAAAAATCTGACAATATTTTGAGTATCCTTGATCAAGA
ATATGCCGTGCCTGGATCTGGTGAAAAAAAGAGTGTAAAAAATTACAAATGTCTCCAGCTGGAGTTTGAAAACACTCGACTAAGGCAAATAAATCATACT
CTTTCTGAGTCTCTTCACTCGAGGACAGCTACCTCTGTGATCTTGGAGGATGAAGGTGTCCTTGACAGCATTGAAAAGTCCTTCAGCAAGTTCCATGCGT
TTCTGGATCTTCTAAAAGATGCAGGCCTGGGGCAGCTTGCAAACCTTGCAGGCATTGACCAGTCAGACTTTGCTCTGCCAGGGGATCCTCAGATGTCTTC
TACTATTGATAAAATAGCCCAGCAGGCACCAATGCCACATCAAGATCAGCAAGAAACATTCTTTATCCATCAGGCACAAAGCAGAGATAAGAAAGGTGAG
CATACAGGCCCATCAGAATCCAATGCTTTGACTGCTGATACACTCCCTTCTTCCCGAGAGGCAGATAAATATTGTACCACCAGCCAGAAGAGCAAAGAAG
GTTTTGATCCTGATCTAAAGGCCAGAGAGGCAAACGTGAACAGTGGGAGGCTTCCTGCTCTGGAGAATGCACAAACTTACTTGTATGATAAAAAAGGATC
TAGAAATGAATGTTCTGCCTCAGATTCCAGCAGGAGCCAAAGAAGTAGCTTAGGTCAGAAGTCCAAAGCATCGGCAAGTGAAAAGGAGAGAAAGCCATCT
TCAAAAAGAAGCCATTCCTCTGGGACAAACAAAGCTAGTCAAGAGTCCATAGCAGCTAGGGATGAAAAGGCTGCGGCCCGTTCTAACGCCAGTATATCAG
AATCTGAAATACAGGAGAAAATGAATTCTTCTGAAAGCATGCACAGTGGCTCACATTAAATAAGTTTCTATTAGAAGGAATAGGCATATTTTTATTATTT
CAGGTTTTTGCATTTTATTAGAATGAGAAAAATAAAATATTCTAGACAGGGTATACGGTGATATGTGTGCTCATTTACTAACATTGGGCACATTGGCACC
TGGGCAGTAACCCATAGCAAACAGCCAGGGATTTTTTTGTTCAGTTAATCAGCTTTTACAATTCAGAAAGTGTAATTGCGTTATGGATGGGGCACATAGT
GTATATCTGCTTCTTTCAGCCTCTTTCAGCGTTTTTCCATCAGACTCAAAGAAGTGGATGCACTTTCCTATGTTGCTCTGTGTGCCTGCACTGAAAACTA
CAGTGTCCACCTGTAAAACGCAAAAGCAGACGTTTTTAGGCAGACTCTGCTCCGCTGTGTGAGGCGTTGTAGAGTGTAGTTTTCAGTACAAGCACACAGA
GCAGCGTAGTAAAGTGGATCTGCTTCCCATTGCCATATCAATTAGGATACAAACAACCAGAATGCAGTTGAAATTTCAAGCCTGAGAGTTGCAGAACATC
ATTGCGCTGCAGCATGGGCCAAAAATAAAAAGGACCATCTCTAGACAAGCTCTACAACTTTTTTTTACATAAATTAAGTGATATAATCCCCTTTATGTGA
AATAGGCAGTTTTTAGCCCTCCGTTGCTTGTGAAAATAGGAGTCCTTTTATCCTGTCTCCTATCCCAGATGTTGGTTGCTGTAGTTTCCCAACAAGTTTA
GTGTCAAATGTTGCCTGACCCTGTCCTAATCAGGCTAGTTTCTGCAAGGCTTTTACTTCATATATACCTGTAATAAAGGAGGTTGCTGACCCCTTGCCAA
CTCCACAGATGTAAGTGCAGAAGTACTAGTGATGCATTAAGTATGAAAGCACTACAGTAAAGCCTTGAGTGGATTGAATCATTTGCACACACATGACCTG
GACTAAAGCCCATCCATACCTTGTAACAGCAGTGCAATATCGGCAATGAACTGAACAAAAATAACTCTCCAGTTTTCACTTATATCGTGTAACAAGTGCA
AATTGTAAGATTTTGCATGACATTGATAAAATTGAGGGAGCCATAGGAGTTGGAGTTACTGATGTCCCAAGTAACGTAAGCAATTACGTTACTTATGCAA
AGCACAAATCCATGAAGTCCAACCTTTTGTCCCAGAAAAACACAAGTTAATTTTTAGTGCTAGTTAATTTTGTGTAGTTTGTACAAGGGGCATAGCCAAA
AATGTTGGTTCTACACACTTGGGGGGGGGTTTGAGTGAGGCCCTGGATAAAATACTTCACTGGTTAATAGTTTATTGAAGGTGTGGATCTCCTGTAAGGC
TTTCCTAGTCATCAAATGGATTTAGTAGTAAAAGGCACATTTTTTTTGCCCAGGTACAGTAAACAATAGTAACCAATTACAAGTTTGCATTTATCCAAGT
GAGCAGTCAATGCTTTTAGCTGCAAGTTGTAACGGCTACGAAAAAGTCGCGACAATTCACGAAAAAGTCGCGACGGCGACGAAAAAAATCACCAAAAATA
CAAAAAAAGTCACAAAATGTTTGTTTCCAATCCGATTTTTTTCCTATTCAAGATTCGAATTCGTGGATTAGTAAATCAGCCCCTATGAGTATTTAGCCAA
ACCAGCCCTGCAGTTTTGATAAGTCACACTGAATTGTAGAAATTAGGGGTTATTTATTTATTTTTTAATGCTATGGATTCAGGATTTAGACACATGTTAC
AATTCAGATGGCTATGAGGCACAGACACTTTATATTCATATTTGCATTTCATTGGTATAAATGACATTTTCTATAGTAGTTGCTTTGGTGAAGCATTATC
CTTGAATTGTAAAGCCAGTGGTGCACACGGGTTTACATTGCCACATTTATCCCTGACAGGGCCGCAGCAGTGTGAAATGCCTGGGTGCTTTTAGCATCAT
TGCAGTGTGTTGTGGACCATTAGGGCTTGTTGTTTGTCCTAACTTTGTATTAATCTTTTTCAACACTGTGAAAATCTGTTTTTATAGAATGAATTTTACC
TGATTTTTTAGAAAAAATTGGATCTGTTTGGGTTTAAAGTGGACCTGTCACCCAGACATAAAAAGCTGTATAATAAAAGCCCTTTTCAAATTAAACAAGA
AACCCAAAATCATTTTTTTATTACCACATCCATACCTATTATAAAAGCATTTAAAAATCCCAGCTGTCAGTTATATATTGCCTGCCCCGCCTCTATGCCC
TTAGGCATAGAGGTGAGGCAGACAGTTACTTTCACTTTCAATTCAGAACTTCTTAGATGTTGCTGCACCCCTCACGTTCCCCCTCCCTCCTCACTATGTA
ATTTTGTAACCAGTGCATGGACATGGGCATTAGGTCCCTCCATACTGGCACAGAAACAAGATATTGGCAGAATGCAATGCCTGCTTTGATAACAGTGTCC
ACAAAATGGCCCCTGCCTGCATGCTGCAATTGTGAATTCCAAGGCTGAAGAAACTAAGATTAAAATAATTTATATAGTGTAAGTAAAGTTTATTTTGCTT
GAAAACCACAATAGAAAACAATTTGGAATTATTTCTTAAGGTGACAGGTCCGCTTTAAAGAGTTTTTACTAGTCGAGATTTAAGAAGGACAGAAGACTGA
ACAAATTTGTTTTTTTTGGTTTCATTTATGAACTTTGTACAATTTTGAACTCTTTGTATATTTTTTATATTAAGAATATTTAAATAAAAAGTTACATTTT
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX894899 [+]tggggaggcaaaacattttattgttagggccccatgtttcctgatggcggccctgactttgcgaaagtaacttataacttgtaagagacaagccgacata
acgagtaagcacaggagaacatcaaatcactgatatgaagcagcagtattcttgatcccagcggtgggaaatgagaggggcggcacttggaaaaggtcga
gccgaggagactaagatgggttggaagggaaggaaccatctctgaccgaagaggtcagtaggaggggagcaggaagccgggcactcggaaagggcgggga
aatggaaggcggttaggaaaccaaggaaacggccccacgctcagcctgcagagtaggcatagggaagcagatggcacagtacgattgttgttgcgggaag
ctgattactacgaaagctgcatgcttactcccgtgagtccgccagcacctaaaagaatttgtgtattcttgaaagatgattgactcagtccgtatacgcc
atcagggttccatggatttcctttcacactatgaatatctgtgtgcacttcaggattctgttccaattccagctgtaaaagcaaacatccgacatggcag
tcttagcttcaatgcagatcggataaaactctctgactggctgcctattctaaattctctgaaaataaataagagtttgctaaatgtgtcaataaaaagt
tgtcaccagcctggacttggggaatttggtgctgagaagtatggaatatatttcanaaggcgcatcccccccatccgatcaaagacatgacttttcagta
tgcaaagcattacaagctgcttgagtgtatcaggctctttaaagagttagagctccatggactgcctctacgagagagag
3' EST: CX894898 [+]ccaaaaaaaaaaaaaaaccaaggggtccaaaatggcccaaggtccaaaaaggaccccaaaaaaaacaatttggtccccccccccgcccccctaaaacccc
gacaagaaaaaccccttaaagggggccccgccccccaaaaaaaaaaccccaaatggtttccctggggggtttcccgccaaaaaaacctttccccccccca
aaaaaatttttttacccctggttccccccccccgggaatccccaatggccccggggggggggggccctttttggggccccggttaacaaaggggggttgg
cttccccccaaaatcttgttccgggcccaaaagggggggcccaaaggccctggccccggccggggttacaaaattccaaagggggggggggggggaaccc
ggggggggccccccccccccaaaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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