XB-CLONE-3604701
Clone Name: IMAGE:7680578
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Gene:
p3h1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC128622 [+]
GCGTGACATCGCGTAGAGGAGCAGTGGCTGGCGAAGTGGTGCTAAAGTTGTTGCCCCTCACAGATATGGCGCTGTCCTTGCTGCTCTCCGGGACAGTTTT
GTTCCTTCTCAGCGCTGAGGTGCTCGCCGCTCGGCTGGAACCCTATGACTCTCTGTATGATAGCGGCGTGCAGGCTTACTATCGGGGGGACTGGCACCCC
GTTATCCTCTTTATGGAGAAGGCGCTGAGGAACCGTGAGCTCCTGCGGCGCCACCACATCAGCTGTCGCCTGCACTGTGCCAACCGCACCGGGGGGTTCA
GCGATCTGCCCCTTCACTGGGGACCCGTGCAAGACCTGACATTGTTCCAGAGGCTGCTGGACCGAGCTGACTGCCTGAAGCGGTGCGAAGAGGAGAACAT
CGGGGCGCCTTCTGACTTCTACATAGCCAGCCAGGAGCTGGAGCTGGAGTTCAGCAAGCGCAGCCCGTACAACTACCTCCAGGTGGCTTATTTTAAGATC
CATAAAATTGACAAAGCCGTGGCTGCTGCATACACCTTTTACATGGCAAACCCAGAACACATGGAAATGAGACAGAACTTGGAGTATTACAAAATGATGG
AAGGTGTCAAGGAATCTGACTTCAGGGATCTGGAAGCAAAACCCCACATGCACGAGTTCCGCTTGGGAGTGAGTTATTATACAGATGAGGAACCGGCGCC
TTCTATTGCACATTTTGAGAAGGCGCTGGAGGAATATTTTGTAGCTGATCTTGAATGTCGGAGCATGTGTGAAGGGCCCTATGATTATGATGGCTACAAC
TACATGGATTACAATTCTGATTTATATCAGAGCATAACAGATCATTATGTGCAAATTATGAACTGCAGGCAGAGCTGTGTAACAGAGCTAGCATCACAGC
CAGGACGTAAAAAGCCTTTTGAAGATTTCCTCCCCTCACATTTAAACTACTTGCAGTTTGCATACTACAACACTAATAATTACACAAAAGCAATTGAATG
TGCAAAAACCTACTTGCTATTCTTCCCTAATGATGAGGTTATGAATCAGAATTTGGCTTACTATGCTGCTGTTCTTGGCGAGGAAGAAGCAAAGTTGATA
AATGCAAGAGAGAATATTAATAGTTATATTCAGCGCAGTCTGCTCGAGAAAGAACTGCTTTACTTTGCCTATGACACCTTTGGAATTCCTTTTGTCGACC
CAGATAGCTGGACTCCAGAAAATGTTGTGCCGAAAAGACTCAGAGAAAAACAAAAGATGGAGAAGGAGACAGCAGCAAGAATTACTGAAGAGATTGGAAA
CCTGATGAAAGAAATTGAAACTCTTGTTGAAGAGAAGAATAAAGAAACATTAGATTTAACAAGGACAATACGAGAAGGCGGTCCTGTGTTGTTTGATGAA
GTCAACGTAGTGATGAATTCAAAGACTTTGAATGGATCTCAAAGAGTTGTTACTGATGGTGTTATCTCAGAAGAGCAGTGTCGAGAACTTTTAGGACTTA
CAAATGCAGCCGCTTCAGCTGGTGATGGGTACCGTGGCACAACCTCCCCTCATACTCCAAATGAAAAGTTTCATGGTGTTACTGTGTACAAAGCACTTAA
GCTGGGGCAAGAGGGCAAGGTCCCTTTAAGCAGCGCACTCTTATACTATAATGTTACCGATAAAGTAAGAAGAGTGGTAGAGTCATACTTTCGACTGGAC
AGCCCACTCTACTTCTCATACTCCCACCTCGTCTGCCGAACTGCTGTTGAAGAGAAACAGGAGGGAAGAAAGGATTTAAGTCATCCTGTGCATGTTGACA
ATTGCATTCTAAATGCAGAAGCAAATATGTGTATAAAGGAACATCCAGCTTATACTTTCCGTGATTACAGTGCCATACTATATCTAAATGGGGATTTTGA
AGGAGGAAATTTTGTTTTTACAGAACTTGATGCAAAGACAATAACTGCTGAAGTGAGACCACAGTGCGGGCGAATGGTGGGATTCTCATCTGGAGAAGAA
AATCCTCACGGAGTAAATGCCGTCACTAAAGGCCAGCGGTGCGCTGTTGCCTTATGGTTCACTCTAGATCCCCGTCATAACGAGAGGGAAAGAGTTCAAG
CTGATGACTTGATAAAAATGCTCCTTAGTCCGGAGGCAGATCTGTCCCAGGAGGAGGCACAACAAACAGCAGAACCACTTTCTCTAGGGGAAGCCAATCT
GAGACAGTCTGGCTCAGAATCACCATCTGAGACCCCTGATGCTAAAGATGCAGATAAACAAACAGATACTACAGATTCAGTTGCTGCAGAAAGCACAGAC
TCTTCTATAGAACACGTGCGCGGACCACCTGTGGACTCCAAACCCAAGACAGAATTATAACATTAATGTGGGACTTTTGGTTGTGCCTCGGAGACTGCTC
TGAACCAGGGAGATAACATTTCCAAAAAAAGTTTAATGAAAAACCAAAGCATAACGTTAATGTTTTTCATTTTGTTTTTAATCATTCCTGGAAGAAAAGC
ACAACGGCAGAGACGACTCATGAGTCATGAATGTTGCATGTACAAATTTACAAAGAGTTTTCAATTCCAAGAGGCAGTTCGTGCTAGAACCTAACAGTGT
GCATTCTGGCTTGCATCTTTTTCTTTTTTTACAGGAGGAAGTTTTTCTGTTTTTAAACCAGGCCCTGCAGTCCCATGCAATAAGTGGATACCAAGGGGAA
ATATCTAGTTTGCTTGTTGTTTCCACTTGTTTACAAGAATGGGTGACAGTTATGATGGAATACCTAATTTTTTGCCTTGATCCTTTCATGGAAAATAGAT
ACTGAACTTTTAATATCGAACATTAGAAAAAATGACCGATTGGGCTCAAAATATTTTTAAATGAATTTTGAAGGCAGAATTTCTTTGTGTCCTGATGTTT
TTGTTGCAGAGATATTCAGTCAACTGTATTTTCAGTGAACAGTGTAATAATCTAGGACCTACACTGGTTGTGTATTTATTTGGAGCCGGTCTTGCAGAAT
AGCCTGTGTTTGCAAAACAACCAAGTGTGAGTTTTTCATGTTACACTGTTCCACATGGTTTGTATGTTTATGTGGTTGCGGAATGATTGAGAGAAATCGG
TCAATGTGCAATTTTATGACTTGAAACTGTGGCTCATATAAATAAAAACTGGCAATGTTTGGGATATAGAGGATGTCTTCTATGCAGGCTAATTGTTTCT
TCCAGGATGTGACACCCTTGCAGGAGGGGGGGTTCTGCGACAGATGAGATTCAATGATGATAAATATAAGTTCTTATGTTTCAGATTTAAAATATGCAAG
CCACCTACATTTTTAATGGGACTATGTTTGGACAGTAACTGTTTATAAACTGCTGTAGCAAGCAATCGCAATGAGCAGCTTGAAGCTGGTAAGAATATGT
AGAATATATGGAGAGTATTCAGTGCTGTTTTAGTCTCCAAGATATTTATGGAATAGAAAATGGCCAAAGAAGAGTAACTAAGCTAATAAATGGCATGGGA
AGTCTCAGTTATGAGGGAATGCAGAACAAGTTAGCATTGTTTACATCATAGGGTGAAAGAGCTCCTACAGTCTCCAACATATTTTACCTAATCTGTGAGC
TTCCTTAGAAAATTCTGCTTATACTCTAAATACTGTAATTCTAATAATTCATTAAAACCCTTAAGAAATTTGGTCTTTGAAGGTGGCCATACACATGGCA
ATTCTGATCTTTCCAGCGACCAACGGTCGCAAGAAAGATCGTTCGTTTCCCCCACTGACGTTCATGACTGATTCGTCTGATGTGGAGGTAGAAACAATAT
GAATTCTATCTCCACCTGCCAATTCAGTCCTGAACATAGATTTTGTTCGGGCGGCATCAAAATCTTTTAACCTGCCCGATTGACGAGACGACCAATATCC
GCAGCCTTTGCGATGTCGTCTCATGGATCCACCATACACGCACCAAATTTTGTACGAAACAAGGTTTCAATCCAATCCAATATTGTTTACATAAAATACA
ATTATCCCCAGCTCTGTCTAGCTTTAACCACCTTTTTTTTGGTGAAATGGAAATTTCAGCATCAAGTTTCTGAAGAAGCTGCTTAGAACTGAATTGTATC
CGTTTGGTTTTCCCACGTTCTGTGTTTGGGAAGTTGATCTTTGCAGTTTGGCAGGTGTGGCTCTGCTGGAGTGCAGTATAGATTGCACCTCTATATCAGA
TCTCAGACAGGGATCTTATTACCCAACTTTGCCTTTCCTTCCACCTTTTAACTTAGTTATCTTCCTAGCCTTTGAACAAATCTTGCTGTCTTCTTCCTGC
AAAAAAAAGAAAATAAAAATTCGCTTTGTGAGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX896209 [+]cgtgacatcgcgtagaggagcagtggctggcgaagtggtgctaaaggtgttgcccctcacagatatggcgctgtccttgctgctctccgggacagttttg
ttccttctcagcgctgaggtgctcgccgctcggctggaaccctatgactctctgtatgatagcggcgtgcaggcttactatcggggggactggcaccccg
ttatcctctttatggagaaggcgctgaggaaccgtgagctcctgcggcgccaccacatcagctgtcgcctgcactgtgccaaccgcaccggggggttcag
cgatctgccccttcactggggacccgtgcaagacctgacattgttccagaggctgctggaccgagctgactgcctgaagcggtgcgaagaggagaacatc
ggggcgccttctgacttctacatagccagccaggagctggagctggagttcagcaagcgcagcccgtacaactacctccaggtggcttattttaagatcc
ataaaattgacaaagccgtggctgctgcatacaccttttacatggcaaacccagaacacatggaaatgagacagaacttggagtattacaaaatgatgga
aggtgtcaaggaatctgacttcagggatctggaagcaaaaccccacatgcacgagttccgcttgggagtgagttattatacagatgaggaaccggcgcct
tctattgcacattttgagaaggcgctgggagaatattttgtagctgatcttgaatgtc
3' EST: CX896208 [+]gggggaaaaaaaaaaaaaaaaaaaaggggggaaaaaaaaaaaaagggggaaaaaaaacccccccgggaaaaaaaaaaaaaggggggaaaaaaaaaggccc
cccaaaaaaaacccccccccaaaaaaaaaaaaaaacccccccccaaaaaaaggggggggaaaaaaaaaagggaaaaaatttttttaaaagcccccccaaa
aaaaaagggggggaaaaatccccccccccaaaaaaaaaggggggaaaaaccccaaccggggggggaaaaatttttttttaaaaaaaaaaagggggggggg
gaaaaaccccccccccaaaaaaaaaggggggggggggggggggccccaaaaaaaacaccccaaaggggggggaaatgggccccccccccc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: