XB-CLONE-3604237
Clone Name: IMAGE:7680791
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Gene:
nid2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC129003 [+]
GGAAGCTCCTGGGTCAGAACTGCTCAGCACACTGGGGCGCGGTGCATATCCTCTTGCCTTGAATGTGGGTCTAGCTGCCATTCACTGCCATGTGGGACAG
AACTCTGCATCTGCTCCTGCCCTTGCACTGCCTGCTGGCCTTCTCTGCTGCTATATCCCGGCATGAACTCTTCCCCTATGGGGAACTGAGGGGAGATGTG
CTCCTGCAGGAGGGGGACGATGAGACTTCTGAGGTGGTGAAACTCTCCCAACCGCTGCTCTTCTATGAAGCCCAATTCAGCAATTTATATGTAGGGACCA
ATGGAATTATTTCTACTCAGGACTTCCCCAGAGAAACTCAGTATGTGGATGATGGCTTCCCTACAGACTTCCCTGTCATTTCTCCATTCCTCTCAGATAT
AGATACCAGCAATGGCAAAGGAAAGATTTTTTATCGGCAGGACAATTCTGAACAAGTTCTCAATCGGGCAGTCAGTGAGATCCAGAGGGGTTTCCCAGAG
ACTTCATTTGTTCCTACTGATGCCTTCCTTGTGACTTGGGAAAATGTTGGTGCCTACGAAGAGATGACCCGTCTCTCTCCATCGTCCGATAGGAGAAACA
CGTTCCAGGCGGTACTAGCATATGATCACTCTGATGCTTACGCTATATTCCTTTATCCTGAAGATGGACTCCAGTTTTTTGGTACTCGTCCTAAGGAATC
CTACAATGTGCACATTGAGCTGCCTGCCAGAGTGGGCTTTAGCCGATCAGAATCGGGACCATACTACAGTGTAACAAGTAATGAGCAATCCGTGAAGAAT
TTGTATCAAACTGGCAACACTGGTGTCCCAGGGATTTGGGTCTTTCACATTGGCAGCATTTGGGAATTAGACAATGTAATTCCAGCAAAATTCTTTGGTG
CTCCCACAAAGGAACAATCGCTTGAAAATTCACATACAGGTTTCCCAGAACACAGAATATTTGAAAAGAATGAAAGTGAAGACAGTATTGATTACCCAGA
GTCCTTTTATGAGGAGAATGAAGATGATCTGGAAGATAATACAAGTGATGCCACACCAACACATAATGCACACCAAAAAGTTGATTCAGACACTGGAAAT
CAGGGATACTTGGACATAGATCAGGCTTCCAAACTGACCCATGCAGATGTTGAACAGGAAGCTACATCATCTGTTGTGCTTTCCTCTTCACATCAGAATC
CTGATTGGACACCCTACAAACTCAGTGAATCTGATACTGACAGAGATGGGCCATTGCAGGAACCTCAAGTTCAGACAGGGGAGGATACAGATTTGACAAG
GGGAGACACATTACTGACTGAAGAGCTGCCATCCCATAAAGCACATAATTTAACACCTCAAAATTCTGAAGATCTCTCAATATATCCTGAAAGTGAAGTT
CTCGTGGGCAGTCACCCAGAACAGGAAAATGATTTTGAAAGAAAGCAGATTGATGCTGATCCTGGACTCCAAACTGATGTGTTTTCCTTTAACCCTGTGG
GCAAGGAGACATGTGAGAGGAACCACGGGAGATGTTCTAGGTTTGCTTTTTGTGCCGATTACTCTGCTGGCTTCTGCTGTCATTGCCAGGAAGATTATTA
TGGCAACGGAGTACATTGTTTGCCAAGAGGGGCTCCACACCGTGTGAATGGTAAAGTGAGCGGAAACATCCTGGTCGGTCAAACACCTGTCAACTTCATT
GCTGCCGATTTACACGCATACATTGTGGCAAATGATGGAAGAGCGTACACAGCAATCAGCCACGTTCCTGATCCTGCATCCTGGTCTTTAACACCTCTTG
CTCCAATTGGGGGACTCTTTGGCTGGCTTTTTGCTTTGGAAAAACCAGGCTTTCAAAATGGTTTTAGCTTTACCGGTGCCAAGTTTGTACACAACATTGA
TGTCACATTTTACCCGGGAGAAGAAACTGTTCATATTACTCAAACAGCAGATGGCTTTGATTCTGAGGGTTATATGAATGTAAAGACAGCAATTCAGGGC
AAAATTCCATATATCCCAGAGACATCGACCATCAAGCTGTCTCCTTATACTGAACTGTATCAATACTCTGGCTCAGTTGTAACTTCGACAGCCTACAGGG
AATACACAGTCATCTCAGAATCAAACGAAGAACAAAAACTGTCTTACCGGTTACGGCAAAATGTCACTTACCAAGAGTGCAGCCATTCCCAGAAACAGCC
TGTTAGTGCTCAAAAGCTTTATGTTGATCGTGTCTTTGCCCTTTACAATAAAGAAGAGAAGGTCCTTCGTTATGCAGTTACAAACTACATTGGATCTCTG
CATGATTCGACTGGACAGGACGATCTCACAGTTAACCCGTGCTATGATGGTACCCACTTGTGTGACACAAGAGCTAAATGCCAGCCTGGTAGTGGTCTAG
AATACAGTTGTGTTTGTGCGTCTGGATACCAAGGGGATGGAAGAGATTGCACTGATGTAAATGAATGTGAAGTTGGCTTCACCCGGTGCGGCCAGAACAC
TGTTTGTGTCAACCTCCAGGGGAGTTACCGTTGTGAGTGCGCCAGTGGTTTCACTTTATCTGGAGACGGGCACAACTGCATCTTGGCTTCACTAATAAAC
CCATGTGAAGATGGAAGTCATACATGTAACAGAGACACATCACGCTGTGTTCCGCGTGGAGATGGTGTATTCACATGTGAATGTTTCCCTGGATTTAACA
AGAGTGGAGAAGACTGTGTTGATGTTGATGAATGCACTGAGCACAGATGTCATCCAGATGCCTCATGTACCAACACCCTGGGCTCTTTCTCCTGTCGGTG
TAACAGTGGTTATGAAGGAGATGGCTTCCAGTGCACTCAAATCTTAGGTCCTGAACCCCTGAAAACCCCCTGTTTAGAAAAGCGCCAGCAGCTACACCCA
AGGGGCCCCCGTCCAGCTGTGGGACAGTTTGTACCAGAGTGTGATGTAGAGGGAAATTATGTTCCATTACAGTGTCATGGCAGCACTGGCCATTGTTGGT
GTGTGGACAAATTAGGTGAAGAGATTGCTGGAACAAGAACACCACCGGGCAGACCAGCCCCAAATTGTGGGGAAACAGGTCCTGAACCCCTGAAAACCCC
CTGTTTAGAAAAGCGCCAGCAGCTACACCCAAGGGGCCCCCGTCCAGCTGTGGGACAGTTTGTACCAGAGTGTGATGTAGAGGGAAATTATGTTCCATTA
CAGTGTCATGGCAGCACTGGCCATTGTTGGTGTGTGGACAAATTAGGTGAAGAGATTGCTGGAACAAGAACACCACCGGGCAGACCAGCCCCAAATTGTG
GGGAAACAGGTCCTGAACCCCTGAAAACCCCCTGTTTAGAAAGGCGCCAGCAGCTGCTTGGACAGCTACACCCAAGAGGCCCCCGTCCAGCTGTGGGACA
GTTTGTACCTGAGTGTGATGCAGAGGGAAATTATGTTTCATTACAGTGTCATGGCAGCACTGGCCATTGTTGGTGTGTGGACAAATTAGGTGAAGAGATT
GCTGGAACAAGAACACCACCTGGCAGACCAGGCCCAAATTGTGGGGAAACAGAGCCTACTCAGAGACCTCAGACAGTGTGTGAGAGGTGGAAGCAGAGTC
TTATTGAGCATTATGGTGGGAAACCAGGTGGAGAACATTATGTGCCTCAGTGTGATCAATATGGTGACTTCAGTCCTCTCCAGTGCCATGGAAACAGTGG
CTATTGCTGGTGTGTGGATAAAGAAGGAAGAGAAATTGAAGGATCTAGAACAGAACCTGGAATGACTCCAGCATGTATACCGACAGTGGCACCTCCAACC
ATGCATCCAACTCCGCGTCCTGATGTGACCCCTCCTGCAACTGGCACCTTCCTCTTGTATGCTCAGGGTCAGCAGATTGGCTACTTACCACTCAACGGCA
CAAGGCTCCATAAGGAAAAAGCCAGGACACTGCTGTCACTTCATGGTTCTATAGTGGTTGGTATTGACTATGACTGCCATGAGAAAATGGTGTACTGGAC
GGATGTTGCAGGTCGTACAATCAACCGCGCAAGCTTAGAACCAGGTGCAGAACCGGAAACTATCATTAACACAGGGTTGATGAGCACAGAGGGCTTAGCC
ATTGACTACCTTCGCAGGACAATGTTTTGGACAGATAGCGGCCTAGACAAGATTGAGAGTGCGCGGTTGGACGGCACTGAGAGGAAGGTTCTATTTGACA
CACAGCTTGTGAATCCTCGAGCCATCACTGTGGATGCGGTTAGAGGGAACCTGTACTGGACAGACTGGAACAGAGAGGCTCCAAAGATTGAATCTTCGTT
TGTAGACGGATCAAACAGGAGGATTCTGGTTAATGACAACATCGGCCTTCCCAACGGTTTAACCTTTGATCCCTTCTCCAAACAAGTCTGTTGGGCTGAT
GCAGGAACCAAGAAGCTTGAATGCATTTTGTCAGACGGAACAGGGCGACGGGTCATACAGAGTAACTTGAATTATCCTTTTAGTGTTGTTGTGTATGCAA
ATTACTTCTACCACACAGACTGGAGGAGGGATGGGGTAATATCCCTTAACAAGGACACTGGGCACATTGTTGAAGAATACCTTCCTGAGCAGCGATCCCA
TCTCTATGGAGTAACAGCTGTGTACCCATACTGCCCTTCAGGGAGAAAGTAATGCAAGAATAAGCTGCAGAGTGGAAATTCATTTTGATGGAAGATTTTT
CTCCTTGCTGCAGTTAAAGGGATGGCAAAGAATCAGCACAAATACTACTTGGAAACTGCAAAGAAAAGCTTTATTTTCTTGCAACAAATATGATTTGTAA
AGTTTTTTTTACCTCAAAATTTTTTTACTACTGTCTTTTTACAGAAATTATTTAGCTGTAAAATAAAAAAATAAGCCTAAGCGTGTTGGCAAGCTTGGCT
CCAAAGAGATGTGCCACATGTACAGAGATCGCACATGCCACAGATTTTTTTTAAAAAAAAAAACAAAAAACTTTTTAACTTGTTTAATATTTGTAACTTG
AGTTTTTTTTTTTCAATGGTGCCTTTTTTATATATTTTTTTTCTCTTCCAATGTAGCTTTTAATATTCTATCAACCCACATTACTGTATACTGTGAACAA
ATATGGCCAGAAACTGCATACATCGCAACAAACAAGTACATAAGGTGGTCCGTTTGTGAAATATAGGAAACTTTGTGCCATACCAAAACTTCACAGATAA
ATTCAATAAATCCATTTCTTTTTCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX896722 [+]gaagctcctgggtcagaactgctcagcacactggggcgcggtgcatatcctcttgccttgaatgtgggtctagctgccattcactgccatgtgggacaga
actctgcatctgctcctgcccttgcactgcctgctggccttctctgctgctatatcccggcatgaactcttcccctatggggaactgaggggagatgtgc
tcctgcaggagggggacgatgagacttctgaggtggtgaaactctcccaaccgctgctcttctatgaagcccaattcagcaatttatatgtagggaccaa
tggaattatttctactcaggacttccccagagaaactcagtatgtggatgatggcttccctacagacttccctgtcatttctccattcctctcagatata
gataccagcaatggcaaaggaaagattttttatcggcaggacaattctgaacaagttctcaatcgggcagtcagtgagatccagaggggtttcccagaga
cttcatttgttcctactgatgccttccttgtgacttgggaaaatgttggtgcctacgaagagatgacccgtctctctccatcgtccgataggagaaacac
gttccaggcggtactagcatatgatcactctgatgcttacgctatattcctttatcctgaagatggactccagttttttggtactcgtcctaaggaatcc
tacaatgtgcacattgagctgcctgccagagtgggctttagccgatcagaatcgggaccatactacagtgtaacaagtaatgagcaatccgtgaagaatt
tgtatcaaactggcaacactggtgtcccagggatttgggt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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