XB-CLONE-3601919
Clone Name: IMAGE:7681982
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Gene:
plekhg4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168567 [+]
cactaggagagcgaagtaacatggcagagaggtaacggggcagtagacagggacctggagctgggtcaaggtggctacatggaattgaggtggaaaggac
cataacccgcagacatggattcccagtctttagatggctgcatccagaagacgctctcatctctttacccacccttcgagtacacagcagctactgtact
ctgccaagtcttggatgtagtggagaaaacttatcgtggggacgggctaagctatctcatcgattttctgattcctgcaaagcgtgttttgcagagcctg
cagcaggaagcttgtttgcagtattgtgggttgctattcagacatgcaggatggcctctgtgtatccatgagaagattgttcttcaactagcatccatag
attggagacttcttcagcctggtgatttttacttccaggttgtgccttacttaagaaagacaccaagaattgtgctaaaatgcctggcacaggattatca
taacattgaggagctggtcctcccagaagtatcctacacatcaattttcaccttggaatggttagaattcatcaactcaggacgaatggggacatctctg
gagaactgtctcctgatgtgtgatgaccagatctttagggttccctgggaaagggttgttcatccagaatttatagataaagcacagcctcctgatgaaa
atgctagtgacaaaaagacaactggattgtacctggaatgcaacaatgagatgtctcagcctctgtggagggatttgacagatgagcatattgaaactcc
tgttaatgtggattatgtctcaagtgaagcttcttccactttggatactgagtttcaagtaaaaacaagcatgtcatccagtgagattgaaggagagtat
gtacaactgtctgatatttcagtgcctcgtttagggcctcagatggggtccctaacacagtctatagccttaaattacaaaagccaacaaaaaacagttg
ctcaacagacccaagataaacaaaaccaagttgtttttctggacagtagaacttataaaacaagtgtagtttcctcagatattatgccaacactttgttc
tctgcagccttccactaaagcaactttttcacataaacctacaactcaacagaatatcgatgttcaggacaaaattgtggacttggcctttggagaatct
atatcaaaaaagcacttgaaacaagaatcttctttctctgctggttctataaagcatagttttgttgaaacttgtattacccaaaacaatcatcagaagc
atgaggacatttcagagattactgttccaggaaaggtcagtgatactggagatactgataagtgcacacaaagctgtgaacagccaagtttacagggagg
taattttagcacatcattatcgttccaacaggaaagcagaagccactttataaatggcactgaggatgtcagagtggtgacagatgccttacaagaacct
cctgcagagcattccaggccacaagagacagtgtcggttgaatattttcagtcttcaaacaaagcaatacctaaagaccttattcattatactataactg
ataaatgtgaccaaagtttgccttcaatggatacaacaaagtgtcagtttgaggaacaaaaatctaaagaaaacacagctgatacagaagaaagtcccaa
aaatagaaatgcaggcagccatatttattcctcagctgagtcaagtgcctttgctgtttctgtgttacaggaagggaataatgtcttaaatctagacgtt
tgcaaagagagtggggagcaggttgatggtgttaataaagaactggtcagcagagatttagagaacatgcaattgcagaatgcgctgtctgtagagcaga
gagagcaggagcctaaaagtgactcccatggaaagatttacactatgttgaacagtgaacaagtgggtacacatctctctatgtgtgtgttgtctgggtc
agataaagtacccctgacattagaaactcttcatgaagttgtagaagatgagcaggacctcgctgactttaacagtgtattgagcaatggtgaattgcca
ccttctgaggatatttccaaaacagctttaggaaatctcaaggaccaggaagaaaggacagatcaagatccacaggagaactgtcagcaaaatatgtgcc
aaacacctgcattgtccaggagaagtaatgctcagcccctgactataatggctcaggatgtgaatttagaagttctgttaagtgaagtggtatatttgcc
cggaacaaaggacaaaagtgggcgtgcagtaataataataaacacgaggaatacagcttggcttaatccacactgtaatgcctcagaattgggtcgttta
ttcctgtacttctacagcattccaaggaaagattgcagaagcctgggcttaacactgcttgtggattcccgccgctgttctcctgttcctgctctgttta
aagcttttcatatagtgcaggctgcagttccaagctgcatccatggtgttttgatgcttgtggagaaggatcttgccctgcatgtggagaagcctcatgg
tatacagtttgatcttcttacatctactaaatccttgcacaaacatatagaggcctcccagcttccactggaatttgatggcttatttcgatactgccac
aaagactgggtaacatttaggatgaaactggagcacctacaagaaaattgccgtaatgcttgtgcatttttgcaggatgccataaaaaatctgcacattg
actctcttccaaataaagctgaggatgctaaacttctcctgcaaaaatttggagagttgatgaggatggttctggaagacacaagacttgtgcagctgca
gaaagagggaggagccattttagcgagactaaggaaagaggagtcatgtgtaacacttactgaggattatagggaggctgtagatgtggcagctgagctg
tataaccaggtggatgaaggggttcacagtcttgttaaagtatccaacaagcgtatacaagagatggaactagtcattgagtttgaggctttccaggaac
aatttcaggaggttggcagttggattgagcatgtaggagaacgtttactaaaggagtgcaataccctggaagattctctggaagttcttcttcaaacaca
gagacaatttaaagaattttatgttgttgcatgtgagtattgcaggcgaggccaggatattatgcagaagatggagaagtggcaggatctttcatccagt
gagacccaaatacacagattaaaattgcaaaggtgtaaagaaaaggttgaagatttttggcggcgcctggaagaatctagatatcagctggagaaatctg
tgacactttatcaattttttgacaaggcttacgaatgggctctggaaaggatgcggcatctggcctcctttagcttggatgactgtagctcaccagagat
gtgcttgtctgcaattgagggcttggagaggtacaaaattcagcatccagaaattagtgaggagaaattcatggagatgaaagaacttgcctgtgagttg
aagattgagaaagccaccaagcaatggaagtttgcctggtctaagtgccaggaggctcgacatgttttggagaaaaagctggaggctgcattacgggcaa
aaagtatgttgtctggggagcaaagctcaccaagagaagctgatggaatggaacagaaagaactattagaaagtaacaggaataagtactttttccaaag
caaaccatacaattcacctctctgcccaagagaaaggagactgggtagagtattgtgcaacaggcctgaaatagggaacacagatgaccatacaacacgc
aaagccagtcccacctcagatgcaggctctacagttacatcagtgtttagaacacgcaaaatgtctctgcagagtatctctggagaggatcttagtctcg
atctcctccatattgccccttcaggctcctcaacccccactgttacaccacgtcccttcacaagaagactgttaagaaaggcacagagttttgacctccc
tggaagtgaagggccaggctatgggtgtcaaagaagactgagtgagccgacaaggcgtggcaacactggagtgttcattaaagggcttgaagtaagtagc
acagaacttgtagaccgaccctgcagcccaagacagacccgtgactggtcagctgagtgtatgtacagtgaaagaaggtgcagtgtgtcctcatctgatg
ggaggagccgcagcagcaagctccgtcacattattgatgaaatggtgacgacagagcgagagtatgtgcgctctttatggtacatcagtgagaattactt
cccagagatggagcgcgtggacctgccccaggatctaagaggaaaacgaggaatcatttttgggaacctggagaagctcagagacttccatagccaatat
tttctaaaggagctggagagctgttgtaatcaccctttgcgagtcagccactgctttctcagacataaagaccagtttgggatgtatgctttatatagta
agaacaaaccacgttctgacgccttgcttggcagccatgggaacattttttttaagaataaacagttccttctgggagacaagatggatctagcttccta
tcttttgaagccaatccagcgaatgagcaaatatgccctgcttttaaaggacctaattaaggagtgtgaagaggcacaagaacaagagcttgcttacctg
cgtgcagctgaggaaatggtgaagttccagcttcgtcatggcaacgacctgcttgcaatggatgccattcgagactgtgatgtaaatttgaaagagcagg
gccagttagtgaggcaagatgagttcacagtttggcttggtcggaagaagtgccagagacatgtttttctgtttgaggatctcattttgttcagcaagcc
taaacgcatcgagggtgggctagatgtctacatctataaacgctcatttaaaactgctgacattgggttaacagaaaattctggagacagtggtctgcga
tttgaaatttggttcaggaggcgtaaatccagtgatacacacatacttcaagcaagcaaccctgaaactaaacatgcatggaccagtgatattgctaaaa
tcctatggcaacaagctaccagaaataaggaaatccgaatgcaggaaatggtttctatgggagttggcaataagccattcttggacataaaacctagtga
cgcagctataaataacagagccgtcgattatatcatgaagggaagaggtgcaagaaccagagcttccattgcagtatccttgtttgatcactcagatcct
tacaagagaggtcaagcacccataacctgcagttcagtatcttctgctggaggaccctcctcttcctccttgctggggccccttaatcttcatacatatg
tcaatcaatctcttctgtcaggagtgttttcaaccagcagaccctttgacactagcagttgccttgaagaggatgaattggagaatgagacaaacagcag
ccagccttccatgaccactgagagctcggagtcttcacagtgtatgtctggtgctgggtccagcggttcagacagtggttgcgtgtctaacattccccag
gacaatgtatcagaggatgctggatctccttctgatgcatctgctccctattccattcaagaaaggagaaacaactccagctcaaggtttccacgagcgg
aaaaaagcaaatttatcaaccgccaatatatttctgctaacccaagccacatatgcttaagcccttccaccatagtctgatcttccttctaacctgtgtg
tttcagtttgaagaagcctttccagtgtatgggacacgtgcttgaggcccataatcaagaatctgtatcagactccaagtctcccatgatgaatttgcac
ccactgcttccagagctcttcagtagtttttcttacactcgctaattagttagccacttacatttatatgtattatttgagaaaatatgtaaatagttta
ataagccagcagctgtagtttagtgtttaacacatgtagcactagtggatcatttagtgcttgattcttggcactcttttgctaaagaagatgaatattt
aggctgcacagtttgtaaaatttgttgaaaaaaagctatttaagtaacctctcaaaaatagaataactttctatatcatttttcattgttgaactggaat
ttgataagtgtcttattttgtaatagttgtacccaaattagaaataatttatttaatggtgataatacttaaatctgaataaacagatttctttgtgttt
tgaaaaaaaaaaaaaaaaa
5' EST: CX899114 [+]cactaggagagcgaagtaacatggcagagaggtaacggggcagtagacagggacctggagctgggtcaaggtggctacatggaattgaggtggaaaggac
cataacccgcagacatggattcccagtctttagatggctgcatccagaagacgctctcatctctttacccacccttcgagtacacagcagctactgtact
ctgccaagtcttggatgtagtggagaaaacttatcgtggggacgggctaagctatctcatcgattttctgattcctgcaaagcgtgttttgcagagcctg
cagcaggaagcttgtttgcagtattgtgggttgctattcagacatgcaggatggcctctgtgtatccatgagaagattgttcttcaactagcatccatag
attggagacttcttcagcctggtgatttttacttccaggttgtgccttacttaagaaagacaccaagaattgtgctaaaatgcctggcacaggattatca
taacattgaggagctggtcctcccagaagtatcctacacatcaattttcaccttggaatggttagaattcatcaactcaggacgaatggggacatctctg
gagaactgtctcctgatgtgtgatgaccagatctttagggttccctgggaaagggttgttcatccagaatttatagataaagcacagcctcctgatgaaa
atgctagtgacaaanagacaactggattgtacctggaaatgcacaatgagatgtctcagcctctgtggagggatttgacagatgagcatattgaaactcc
ctgtaatgtggattatgtctcaagtgaagcttctccactttggatactgag
3' EST: CX899113 [+]caaaacacaaagaaatctgtttattcagatttaagtattatcaccattaaataaattatttctaatttgggtacaactattacaaaataagacacttatc
aaattccagttcaacaatgaaaaatgatatagaaagttattctatttttgagaggttacttaaatagctttttttcaacaaattttacaaactgtgcagc
ctaaatattcatcttctttagcaaaagagtgccaagaatcaagcactaaatgatccactagtgctacatgtgttaaacactaaactacagctgctggctt
attaaactatttacatattttctcaaataatacatataaatgtaagtggctaactaattagcgagtgtaagaaaaactactgaagagctctggaagcagt
gggtgcaaattcatcatgggagacttggagtctgatacagattcttgattatgggcctcaagcacgtgtcccatacactggaaaggcttcttcaaactga
aacacacaggttagaaggaagatcagactatggtggaagggcttaagcatatgtggcttgggttagcagaaatatattggcggttgataaatttgctttt
ttccgctcgtggaaaccttgagctggagttgtttctcctttcttgaatggaatagggagcagatgcatcagaaggagatccagcatcctctgatacattg
tcctggggaatgttagacacgcaaccactgtctgaaccgctggacccagcaccagacatacactgtgaagactccgagctctcagtggtcatggaaggct
ggctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: