XB-CLONE-3601012
Clone Name: IMAGE:7682595
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Gene:
nek7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122922 [+]
GGCTGATACATGCTAAATGAAACCAAAAACAGTCTGCATTATATTTAACCATAGCTTAAATAGAAGTCTACGCCTGAGAAGCAGTCTTAGCTGTGCACAG
TTTGTGTAAACAACGCAATGGAGGAGCAGTGTCAGGCCATGCAAGGACCTCCTGTCCCTCAATTCCATGCACAGAAAGCATTACGACCAGACATGGGTTA
TAACATACTTGCAAATTTCCAAATCGGGAAGATGATTGGAAGAGGCCAGTTTAGTGAAGTTTACAGAGCAACATGCCTACTGGATGGTGTACCTGTTGCC
CTGAAAAAAGTCCAAATTTTTGATCTGATGGATGCCAAGGCTCGAGCTGATTGTATCAAAGAGATTGACCTCCTTAAGCAATTGAACCACCCGAATGTTA
TCAAATACTATGCATCTTTCATTGAGGATAATGAACTGAATATAGTATTGGAATTAGCAGATGCCGGGGATCTTTCCAGAATGATAAAGCATTTTAAGAA
ACAAAAGAGACTGATCCCAGAAAAAACAGTATGGAAATACTTTGTGCAGCTCTGTAGTGCCTTGGAACACATGCATTCGCGCCGTGTCATGCACAGAGAT
ATTAAACCAGCCAATGTATTTATAACAGCCACAGGGGTGGTGAAGCTGGGAGATTTAGGCCTGGGCCGCTTCTTCAGTTCTAAAACCACAGCTGCACATT
CTTTAGTGGGAACCCCTTATTACATGTCACCTGAGAGGATACATGAAAATGGATACAACTTCAAATCTGATATTTGGTCTTTAGGATGTCTTCTTTATGA
GATGGCTGCATTGCAGAGCCCATTCTATGGAGACAAAATGAATCTTTACTCTCTCTGTAAGAAGATCGAGCAGTGTGACTACCCTCCCTTACCATCTGAC
CACTACTCTGAGGAGCTGCGAATGCTGGTGAATACCTGTATCAACCCAGATCCAGAGAAGAGACCAGATGTCACCTACGTTTATGAAGTTGCAAAGCGGA
TGCACGCTCTAACAGCCAGTAGCTAGATCAGATTCAGCGCCAGTAGTTTTCCAGTTATTCTTATACTTTCAAATGTTTTGAACCACACAGCCATCCGTAA
TACCTACTGTTATTAGCAAGAAAGCCACACTGTTGAAGGAACCTTCATTAAGCGGAATCCTGCTGATACAGCTATTGATCCAAAAACCTCTCCCAGGTTC
TGCTGGACACAATATCCTCTCCCTCTGGCCCTGTTTCCCTCCAGGGATAAAAGACCAGATTAAAAATAAAAAAAGGCTGTTTTTATAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAA
5' EST: CX900067 [+]gctgatacatgctaaatgaaaccaaaaacagtctgcattatatttaaccatagcttaaatagaagtctacgcctgagaagcagtcttagctgtgcacagt
ttgtgtaaacaacgcaatggaggagcagtgtcaggccatgcaaggacctcctgtccctcaattccatgcacagaaagcattacgaccagacatgggttat
aacatacttgcaaatttccaaatcgggaagatgattggaagaggccagtttagtgaagtttacagagcaacatgcctactggatggtgtacctgttgccc
tgaaaaaagtccaaatttttgatctgatggatgccaaggctcgagctgattgtatcaaagagattgacctccttaagcaattgaaccacccgaatgttat
caaatactatgcatctttcattgaggataatgaactgaatatagtattggaattagcagatgccggggatctttccagaatgataaagcattttaagaaa
caaaagagactgatcccagaaaaaacagtatggaaatactttgtgcagctctgtagtgccttggaacacatgcattcgcgccgtgtcatgcacagagata
ttaaaccagccaatgtatttataacagccacaggggtggtgaagctgggagatttaggcctgggccgcttcttcagttctaaaaccacagctgcacattc
tttagtgggaaccccttattacatgtcacctgagaggatacatgaaaatggatacaacttcaaatctgatatttggtctttaggatgtcttctttatg
3' EST: CX900066 [+]ataaaaacagcctttttttatttttaatctggtcttttatccctggagggaaacagggccagagggagaggatattgtgtccagcagaacctgggagagg
tttttggatcaatagctgtatcagcaggattccgcttaatgaaggttccttcaacagtgtggctttcttgctaataacagtaggtattacggatggctgt
gtggttcaaaacatttgaaagtataagaataactggaaaactactggcgctgaatctgatctagctactggctgttagagcgtgcatccgctttgcaact
tcataaacgtaggtgacatctggtctcttctctggatctgggttgatacaggtattcaccagcattcgcagctcctcagagtagtggtcagatggtaagg
gagggtagtcacactgctcgatcttcttacagagagagtaaagattcattttgtctccatagaatgggctctgcaatgcagccatctcataaagaagaca
tcctaaagaccaaatatcagatttgaagttgtatccattttcatgtatcctctcaggtgacatgtaataaggggttcccactaaagaatgtgcagctgtg
gttttagaactgaagaagcggcccaggcctaaatctcccagctt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: