XB-CLONE-3598942
Clone Name: IMAGE:7683999
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Gene:
pak4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127316 [+]
GACGTCGGGGGCGCGCACGTACTGAGGGGTTAGTCGCTTATCGCCTTTCTTCAAAAATGCGTCCATTGCATGGAGAGGAGTTTTATTGAGGATTTTTTTG
GCTAAATGGAAGGTTCATTAGCTACCATGTTTGGTAAGAAGAAAAAGCGTATCGAGATCTCTGCTCCATTAAATTTTGAACACCGGGTGCATACTGGCTT
TGATCAGCAGGAACAGAAGTTCACAGGTCTTCCTCGACAGTGGCAAAGCTTAATTGAGGAGTCTGCCAAGAGACCAAAGCCACTGGTTGACCCATCCTAT
ATCACAACAATTAATCATGTCCCTCAGAAGACTATTGTTCGTGGCAGCAAGATGTCATTGGATGGGTCCTTGGCATGGCTCCTGGATGAGTTTGATGCCA
TGTCAATTTGCCGCTCAAACTCTTTGCGTAGAGAGAGTCCTCCATGTCACCCCCGAGAGGACCGGTTTCACCAGGAAAATGGTATGAGTGAAGTCCGTGT
ACGGCAGCAAAGGGAAGATGGACAAAGAGGGTCAGAGAGAAACAGATCAGAACATTCCAGAAGAGAGAGACAAAGAGAAGAACACAAAGCAGTTCCCCAG
CAACCACGAGGACAAGAACCAAGCAATAAGCACAGACCGCCACCACCAGACTATCCTAAAGATATACCTGAGAAAAGAGGCACACGCTCGCATGAAAAGG
GTGATGGGAGAAGAGAATACCCTGTAAATGACCGTGGCTATAGTGAACCTGTAGAACGAGTAGTAAAGAGGGAAAAGGGAGAAGAGAAGAGACCTAAGTC
ATCATACACAAGTGCAGAGGGCAGCCCGCAGTCTCCAAGAGACAAGAGGCCTCTCTCTGGGCCCAGTATTCGCACTCCTGGTAGTTCTTCTACAGAGGGA
GTAGTAAAACAAACACAGACAGGAAGGGCATTCAATACCTACCCCCGTGCTGAAACTGACCCCAGTAGAGGGGCACCTCACCAGGTGCCTGATTCTCATT
CTACTGTTCCACTTGACTCCAAAAGCACTGTAAGCAAAAGCTCTTCTCGTCCTCAGCAAGGACAAGTCAAGCCCAAGCTTCCAGACAAGCCTCATCCTAC
ACAGCTTGTCCCACACAGCTCAGATCCTCAGCTCTCCCATCCTGTTCAGACCCAGCATCCTGCCCAGCCCCGTTCTCCACAGAGGGAGCCACAGCGCGTG
TCTCATGAGCAGTTTCGGGCAGCCCTGCAAATGGTTGTGGACCCTGGTGATCCAAGGACTTACTTAGATAACTTTATTAAAATTGGTGAGGGCTCAACAG
GAATTGTTTGCATTGCTACCATAAAGAGCAGTGGGAAACTTGTAGCTGTAAAAAAAATGGACCTTCGTAAACAACAGAGGCGAGAACTACTCTTCAACGA
GGTTGTTATAATGAGGGATTACCAACATGAGAATGTGGTTGAGATGTACAACAGTTACCTTGTGGGGGATGAACTATGGGTTATTATGGAGTTCCTGGAA
GGAGGTGCACTTACTGACATTGTAACACACACCAGGATGAACGAAGAACAGATAGCTACTGTCTGCGTGTCTGTGCTAAAAGCTTTGTCTGTACTTCATG
CTCAAGGCGTCATTCACCGAGATATTAAGAGCGATTCCATACTACTAACACATGATGGACGGGTGAAACTTTCAGATTTTGGATTCTGTGCTCAAGTTAA
TAAGGAAGTTCCTCGACGCAAATCTTTAGTTGGCACACCTTACTGGATGGCTCCAGAGCTAATTTCGCGGCTACCATATGGACCAGAGGTTGATATTTGG
TCTCTTGGAATAATGGTGATTGAGATGGTGGACGGAGAACCTCCTTATTTTAATGAGCCTCCACTAAAGGCTATGAAAATGATTCGGGACAATCTCCCCC
CCAAGCTAAAAAATGCACAGAAGGTATCTCCATTGCTAAAGGGATTTTTGGATCGACTTCTTGTTAGAGATCCCAGCCAAAGAGCTTCAGCTAATGAGCT
TCTTAAACACCCCTTCTTGGGGAAAGCTGGACCGCCATCTTGTATTGTCCCACTAATGAGACAGAATCGTTTGAGATGAAGAGAAGATGACACTTGATAT
TTACCCCACTGTGCACCACCAAAGATGAAGAAGAATGAGAGAGGAGAACATCAATTTGTCACACTATCACTTTATCTCAGTGTTGTATATACTACTGAAC
ATTCCTCTTCCAACATCTCCTCTCATTTTTCTGTTTTTTTGGAGCATATAGAAAGATGATGTTAAAATATTTCTCCAGTTTAAGGGTTATGAGGTAATTT
ATTGTTAATATCCCTGGACATCTGACCATCTTGCTAATCAGTGAAATAGTGGTTAGATAAGAGAAGCATAGAGTTTGGCACCTGGTTCTTTTGGATTCAG
TACCACTCAGAATAATAGTGAATCAGACTGGATCTCTTATAGCACTTGGACAGACACAAGCATACCCAATAATTTACCTGGGACTAAACACTGGAAACAT
TTATTGAACGTTGGTCAGGGTGCGACTGGAATTTTCCCCTTGGAGGACAGAAGTAATATTTGTCTAAAATATTGAGAAGAACATCTCAAGGGATTAATAG
GATCTTTATTTGAGCTGGCTCTGTTAATTACTTTATATTGCCAATGTTTTTAGTACACCTAACTGGATTGCATTAGTGTGGGGGTGCCTAAGGGTTCTGT
ACAGCCAACAGCTTGTTCTGTCTGGATCACACTCTCTGTAAAAGGGCACTTAGAGTATCTTACAAGATTAACCACCATATATGATATTCATAGATCTATG
CATGATCTGTCTGTATGGCTGGTTACTTTCCTGGACCATTAATGGTTATGAATTTTCACAGTGGCACCAAGATTGTAGATTATTATCCTGGTCTTCCAAA
ATGACACTCATTACACACTTCTGGCCTTTGCTTTCACTACTATTGTCATCTCTCTTTAGTGGGGTCCTTCTCATAGATAACACTAATCTGTTCCAATCCA
CATGATAAAAGTACTTCTGCCACTACAGAGCATGATGTTACTAAGGAGATTTCCGATTACATTGGGTGAGCATGCCGAGCATTGTCACAACATTTACTGT
AGAGAATACACAGACTATAAGTGGTTATTACTGAGATAGTAGGGTTACTACTTTATTTATTATGAGAGATCCCTTATAGGGATGTAATAGTTTAATTATG
GAGTATGCCATGCCACTTTATAAACTGTGTGGGAAAACTGCATCTAGTAGTTATGAAAGGCCACTATTTACTCATGAGAATCGTTTGCATGGTTGACCAT
ACTGTAGAATAATATGTTACCCCTTTTACCATATCAGAGGCCTACTATAGAATGAAAACTTTACTGTGGAGGGGATACTGTGATAATACAGGATAACAGA
TCATTCCTTTTAAACACTACAGACTTAGTATAAAAAGAGCAGTTCTATGGTGTTATTTACTAAAAATGTAATCTTTCAGTGCATCATACAATGAGAATTG
TTAAAACATTCATTTTTGTTTTCATGATCCCTTTACAGAGATGTGTATTATTGAAAGTGTTAAGTCTACACATAATCATAAATATGGAGGTAACAATGTC
AATTAATGAGGGAAACAATTTACATGGATATTATACGAGAATTACAGAGTTAGCTGTGTCTTCACAGGTACTGAAATTTGCACTGCATATGGAGATGTCA
GATTTTGTCTACATAGATTTATCTTTAGTGTTACTCTGTGCAGTTTATTCTCCCCACTAGAAGTGTATTTTAATAGAAATCTAAAAGCCACAAATTCATT
TACTGGTAAAATTAAGGTTACTCTCCCAGATTAAGTGTTAAAGCCTTCCATATTCTTTCCATAGTCCCACAAAATCTTGTCAAAATCTCCGGTGTAATTT
AGCCCTAGCTCTCAGTTCTTGTCCTGTTTATATGTTCTGATAAACAAGTTCAAAACCCACACCACTTTTAAAATTAGGAGAACAAAAATCTGCAATGTTG
CTTTGCTTGACACATTTATTACACAGCAGTAGAATCAACAGTCATATTTGTGTTTTACTATAACTGAAAATATTTAATTTTAAAACATAAACAAGGAGCT
TGTAGATCATTATACACACAACAGGCAGTAATCTGTTGGAGAGTCCACTTGCATCTTTAAGTTAGGGGTGGCTCTGCAGATATGTGGATAATGGGAGTTC
TTTATGTCAAGCTTTAAATAAAAGTTTTGCTAAAGAAGACAAGGTAGGGGACAAGAGATCCTAAACTGTGTGAAAAGAACCTTGATGGGTAGAGGTGCCT
TAGCAGCCTAAGGAGCAGTGTGGTGAGATTTAGGGGGCATGTTAGCTCCAGATACTCCTGGAAGCCCAGCAGCAGAAGGTCACCTATTGAACACCTATTT
GCTTCCCCTAATATTTCTAGAAACATTCATGAAATGCTGACACAGCATTGTTTTCATAATATGTTCCTAACTATGGTTTAAGGGAGGACCTGACCAATAT
TTGAAATTCAGAGGAGAGGTTCTGACCAAAAAAGTCCAAAACCCCACTGCTCTAGTACTACTTTGATATCAAAGTGGTCGAGACTTCCATTTAAAGTGCC
AGTAAAATCAAGAATAGGAAATATTATATATATGTTTATAGATTATATCTCTGTTCAGTTTCAGCATTTTTTTATAGTAACATAACAGTCCTTGTATAGC
AGACTTTCATACTGTGAGTATGTTATGTATTAATGACATTTAATCCCCTGTTTAGTGAATGGGAATAGGTACTGTGTGTGCCAAAAGCCTTAGTGCTTCT
GAAGGAATTACTTAATGTTTACATGTACAGGGTATCGGTAAAAAATGCAACTATATATGAAGCCCTTTCAATTTTTGGTTTTACTGGCCCATTAAGGGAA
AGCTTCTGATAAGAAAGGGGTACTAGTCTGCAAAAATGTAAACATACAAAAAGGGGATTTGAGTTTTATCCATCTCATTCTCTGTGGAGGTATCTATAAT
TATATGGCATGATGGTCTGACAAGTAAGTGTTTTCTATTTAGAACCAAGCTAAATTCTAAAAAAAATTGTACTATATGGCATTATGGTTGGTTTGTCTGT
GCATTCAATTTAATGCTACCATACAGAAATCGCACATGGACTACATTTCAAAATTGAGTCTTTTATGGTTTCTTTTTGGATTGGGTGTGAAAAGTTTTTC
TGCTGAACTCATTTGTCAATCAGCCATTTCTCTGTATCACAATCAGTAGTGCCTTACTGCATTTGCATTTTAACAGGTGATCTAGCCTTTGACATAAGAC
CACATAAGCTAGCCTGACAATCATATAGCAACATGAAGACTAATGTGAAAGCAATAGTCCTAAGGAGCATTGTAACCCACTTGTAGTGTTGGGAGAGCTC
CTGCAATCCCTCATTGTCCCTTTCATCCCTTTCTGATTGAACACTGGGTATAGTTTTATACATAAATATATATTTATAAAGTGTATGTTTTGGAAAACAC
TGTAGTAATCATGCATTTGAACAGAAAGCAGAGCTGTGACACTAAGGACTGCTTTTCAATTGATTTGTAATAAGATATTCAACTGTATTTTGCATTTCTT
TGAACAAAGTTGAAATAGAAGTACTAGTTTTACAAATACAGTTTTGGACTAAAAAAAAAAAAAAAAAAAA
5' EST: CX902201 [+]cgtcgggggcgcgcacgtactgaggggtntagtcgcttatcgcnctttcttcaaaaatgcgtccattgcatggagaggagttttattgaggatttttttg
gctaaatggaaggttcattagctaccatgtttggtaagaagaaaaagcgtatcgagatctctgctccattaaattttgaacaccgggtgcatactggctt
tgatcagcaggaacagaagttcacaggtcttcctcgacagtggcaaagcttaattgaggagtctgccaagagaccaaagccactggttgacccatcctat
atcacaacaattaatcatgtccctcagaagactattgttcgtggcagcaagatgtcattggatgggtccttggcatggctcctggatgagtttgatgcca
tgtcaatttgccgctcaaactctttgcgtagagagagtcctccatgtcacccccgagaggaccggtttcaccaggaaaatggtatgagtgaagtccgtgt
acggcagcaaagggaagatggacaaagagggtcagagagaaacagatcagaacattccagaagagagagacaaagagaagaacacaaagcagttccccag
caaccacgaggacaagaaccaagcaataagcacagaccgccaccaccagactatcctaaagatatacctgagaaaagaggcacacgctcgcatganaagg
gtgatgggagaagagaataccctgtaaatgaccgtggctatagtgaacctgtagaacgagtagtaaagagggaaaagggagaagagaagagacctaagtc
atcatacacaagtgcagagggcagcccgcagtctcca
3' EST: CX902200 [+]agtccaaaactgtatttgtaaaactagtacttctatttcaactttgttcaaagaaatgcaaaatacagttgaatatcttattacaaatcaattgaaaagc
agtccttagtgtcacagctctgctttctgttcaaatgcatgattactacagtgttttccaaaacatacactttataaatatatatttatgtataaaacta
tacccagtgttcaatcagaaagggatgaaagggacaatgagggattgcaggagctctcccaacactacaagtgggttacaatgctccttaggactattgc
tttcacattagtcttcatgttgctatatgattgtcaggctagcttatgtggtcttatgtcaaaggctagatcacctgttaaaatgcaaatgcagtaaggc
actactgattgtgatacagagaaatggctgattgacaaatgagttcagcagaaaaacttttcacacccaatccaaaaagaaaccataaaagactcaattt
tgaaatgtagtccatgtgcgatttctgtatggtagcattaaattgaatgcacagacaaaccaaccataatgccatatagtacaattttttttagaattta
gcttggttctaaatagaaaacacttacttgtcagaccatcatgccatataattatagatacctccacagagaatgagatggataaaactcaaatcccctt
tttgtatgtttacatttttgcagactagtacccctttcttatcagaagctttcccttaatgggccagtnaaaccaaaaatgaaaggggctcatatatagt
tgcattttttaccgataccctgtacatgtaaacattaagtaatc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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