XB-CLONE-3598519
Clone Name: IMAGE:7684351
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Gene:
acsf3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122927 [+]
GAAGGACAGAGGAAATGTTGCTGAGAAGAGTGCTGGGCTAGTTGAACGAGAAAGCCAGTGACAAAGCCAGTCAGCAGCTACCAGACCAGACTGACGAGTG
AAAGCTAATATATCTCAACCCCATGGCATGACTCACCGATGACATCAGCTAACTGTACTTTAGGATTCATAAATGAAATTATTTGCTTTTCGCAGCCTGA
GGATTATAGAGGCATCCTAGACTGAGCCATGGCAGGAGGGAGCTGCCATTTTGTTTCACAGATGCGGGTTGCATCCTTTTTGGGGAGATTTCTTTATACT
TGGTCATGTGTCCGTGGGGTGCAGGTGAGAACAAGGACCCCACTGCATTCTTGCTACTTTCATGTTTCAAAATCTAGCACTTCATCTCATGTGGTTCCAG
TTTTTTCCAGAGCACCCTTTTTTAGTGAAAGAACAGCTATGGTGGACCAGCATGGGAAGCATACATACAAGGAGTTATATATCAGGAGCCAAGCTTTATC
TAAAATGATCATCCAGCTTTTGGGTCATCCAAGTCGTAATAATCCACCAGAAAGGGTCTCTTTTCTCTGTCCAAACAACTCGTCATATGTAGTGTGCCAA
TGGGCAGTATGGATGAGTGGTGCCATTGCTGTGCCTCTCTACAAGAGTCATCCACCTTCTGAGCTAAAGTATATTTTGCAGGATTCCCAGAGTGCTCTGG
TTGTGGCAGAGGAAAGTTATGCAAATGTAATGAACCCATTAGCAGAGCAGTTGGGAATTCCAGTGCTGACAATGTCTGGGTCTCAGAGCCTTCACCCTTC
AGAACTGCTGCAAGAAATCGAAGTATCAAAACTTGAGTTGGACTGGAAAGACAGAGGAGCCATGATAATTTACACAAGTGGAACCACTGGAAGACCAAAG
GGTGTTCTCAGCACCCATTACAACCTATATTCTATGGTTTCAGCTCTGGTCACTGAGTGGGGTTGGACAAAAGAAGACAGCATCCTTCATGTACTGCCCC
TTCACCATGTTCATGGGGTGGTAAACAAGTTGATGTGCCCCTTGTGGGTAGGAGCAACCTGTGTGATGCTACCAGATTTTTGCCCACAAAAGGTCTGGCA
GCACTTTCTCAGGAGAGATGCACCCTCTATCAACATTTTCATGGCAGTGCCAACCATCTACAGCAAGCTGATTTCTTACTATGAGCAGCATTTTACCCAT
TCCAATGTGCGAGAGTTTGTGCGGGCAGCATGTCAGGAGAGAATCAGATTAATGGTGTCAGGCTCCTCGGCCCTCCCTGTGCCGATTTTGGAACGTTGGC
AAGAGATCACTGGGCATACCCTGTTGGAAAGATATGGCATGACTGAAATCGGGATGGCTTTGACCAATCCACTCCATGGACCCCGTGTGCCTGGTATATG
TGTGCAGTTGGTAACCCTCTGCCGGGGGTGGAAGTACGTACTGTTACCCAGAACCCACGGAAAGAGGGCACCTCCTACACTGTCCATGCTCATGGAGATT
CTACAGGCACCATGGTCTCTGTTGGTTTGGAGAATAGAGAAGGGGAGCTTCAGGTTCAGGGGCCTGCAGTGTTCAAAGAATATTGGAACAAACCACATGA
GACGCAGGAAGCTTTTACCTCAGATGGCTGGTTTAAAACAGGGGATACAGCTGTGTATAAGGACGGCACATACTGGATACTGGGCCGGACATCTGTGGAC
ATTATTAAAAGTGGCGGATATAAGGTCAGCGCCTTAGAGGTAGAGCGCCATCTCCTGGGACATCCCAGCATTACTGATGTGGCTGTGATCGGAGCTCCAG
ATGTGACATGGGGGCAGCGTGTAGCAGCTATTGTTAAACTGCGAGATGGACATGCATTGTCATTGCAGGAACTGAAGGAATGGGCCAGGGCAGTCATGGC
CCCGTACTGCATTCCTGCTGAGCTCATCCGAGTGGAAGAAATTCCCCGAAACCAGATGGGAAAAATCAATAAAAAGCAGCTGCTGGTTCATTTCTATCCA
CAGTAAGGAGAGGACTGGAGTATGGAGTGGAGCCAGAGTAGCCCCCTGCATATACAGAGAGCTCTTCTTGATAACACTAAAATGATCCACTATTACACCT
ATGCTTTTATCAGCCTAGTAAATCATGCACACATAGAACCCTACTGGGCTGAAATGTTATTCTTTCTGTATAATGTTACCATACCTGTCTTCAACAAAGT
TTCACTTGCCTATTTGTATTTTACTGAGAAGGTAATTATGGAAGAAATAGGGCACGAAGGCACTCTGTATTGGCGTGTAAGCAGGACTCTCTAGCATCCC
ACATTTTACACCAAAAGAGACAAATTTATCTTGGAAAAAGTATCCCCACCCACACAACCTAACTTATGAATGTACACCAGTCTTTTCTGGGGCTACCATC
CCCCATCTTTCTAAACACAGTGCAAGATATCCTGTGTATGAATGATTTTGATCATAGATGGAAACTATAAAATTATTGCAGTGATAATGTTATCTTTAAC
TTTTCTCTTTGACTATTGAAGGCCTGTAAAATGGACCACTGTCAGTGCCATCAGGCTTAGTAAAACTGAGGTTTCATTCTCTAAACTGTTAAAAAAAAAA
AAAAAAAAAA
5' EST: CX902643 [+]aggacagaggcaatgttgctgagaagagtgctgggctagttgaacgagaaagccagtgacaaagccagtcagcagctaccagaccagactgacgagtgaa
agctaatatatctcaaccccatggcatgactcaccgatgacatcagctaactgtactttaggattcataaatgaaattatttgcttttcgcagcctgagg
attatagaggcatcctagactgagccatggcaggagggagctgccattttgtttcacagatgcgggttgcatcctttttggggagatttctttatacttg
gtcatgtgtccgtggggtgcaggtgagaacaaggaccccactgcattcttgctactttcatgtttcaaaatctagcacttcatctcatgtggttccagtt
ttttccagagcacccttttttagtgaaagaacagctatggtggaccagcatgggaagcatacatacaaggagttatatatcaggagccaagctttatcta
aaatgatcatccagcttttgggtcatccaagtcgtaataatccaccagaaagggtctcttttctctgtccaaacaactcgtcatatgtagtgtgccaatg
ggcagtatggatgagtggtgccattgctgtgcctctctacaagagtcatccaccttctgagctaaagtatattttgcaggattcccagagtgctctggtt
gtggcagaggaaagttatgcanatgtaatgaacccattagcagagcagttgggaattccagtgctgacaatgtctgggtctcagagccttcacccttcag
aactgct
3' EST: CX902642 [+]aacagtttagagaatgaaacctcagttttactaagcctgatggcactgacagtggtccattttacaggccttcaatagtcaaagagaaaagttaaagata
acattatcactgcaataattttatagtttccatctatgatcaaaatcattcatacacaggatatcttgcactgtgtttagaaagatgggggatggtagcc
ccagaaaagactggtgtacattcataagttaggttgtgtgggtggggatactttttccaagataaatttgtctcttttggtgtaaaatgtgggatgctag
agagtcctgcttacacgccaatacagagtgccttcgtgccctatttcttccataattaccttctcagtaaaatacaaataggcaagtgaaactttgttga
agacaggtatggtaacattatacagaaagaataacatttcagcccagtagggttctatgtgtgcatgatttactaggctgataaaagcataggtgtaata
gtggatcattttagtgttatcaagaagagctctctgtatatgcagggggctactctggctccactccatactccagtcctctccttactgtggatagaaa
tgaaccagcagctgctttttattgatttttcccatctggtttcggggaatttcttccactcggatgagctcagcaggaatgcagtacggggccatgactg
ccctggcccattccttcagttcctgcaatgacaatgcatgtccatctcgcagtttaacaatagctgctacacgctgcccccatgtcacatctggagctcc
gatcacagccacatcagtaatgctggga
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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