XB-CLONE-3598504
Clone Name: IMAGE:7684353
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Gene:
eif4g3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127320 [+]
GCCGAGGGTCCGTCCTGCCGGACTGCTGGAGGCGGACGCAGCGCCATGTTGGATGCTCCGCTTTCTGAGTGAGGAAAATCCGCCGGCTCTGAGCATCCCC
GAAGCGACAGCGACAGCCGCCCCCCCGGGCCGTGTCCCTCCCCCATGAGGATCGGGTCTTGAGCCCCCTGCTGCCTCCCTATTGCCCCTGTGCCCTGGGG
GGCCCCGCTCTGCCATGAGTCTCCCGCAGAAAGCGGCCCTCAAGCCCGCCGCACCGACCGGCACCGGAGCCCTAGTGGTTGGGCCTCCCCCCAGTGCCGC
CCCTCCCGCCGCAGCCCCCTCCGCCATCCGGGCCCTTCAACCGCCGCCTCAAATATCCAGGGGAGCCCTGCAACAGAGCCTCGATGAGCGAATCTTCTCC
CCCTCCGCCGTTTCTGCCGTCTACAGCACAGTTGCAGCACAGGTAGCCAGGCAACCCGGCCCTCCGGCCCCTTCTCCTTATTCTACTCATGATTTATCCA
AGGGACACCCTAATCTGGCTGCCACCCCTCCAGGGCATGCCGCCTCCCCAGGATTGTCACAGGCTTCCTATGCGTCGGGCCAGAGCCCAGCAGCACCCAC
CCTGGTGTACCCTCAGCCACCGCAGACTATGAGTACTCAGCCCCAAAATCGCTCACCATTTGCAGCTGGACCCCGACCTGCCCATCACCAGTTTTTCCAG
AGGACCCAGATTCAGCCTCCCCGTGCCGCCATCCAGACTAGTAGCCCCTCCATCCGGGCCGGGGCCCAGACCCCAACTGCGGTCTATCAAACCAACCAGC
ACATCATGATGGTCAATCATCTGCCGATGCCATATGCAATGCCCCAGGGGCCACAGTACTGCATTCCCCAGTACCGACACAGTGCCCCCCCATACGTGGG
GCCTCCCCAGCAGTACCCAGTTCAGCCGCCTGGTCCTAGTCCGTTCTACCCCGGCCCGGGGCCCGGAGACTTTCCGACCCCCTATGGAGCCCCGTTCTAC
TCGAGCCAACCCGTGTACCAGTCTGCGCCCATCATTGTGCCTACCCAGCAGCAGCAGCAGCCGCCTCCCCCTGCCAAGCGAGAGAAGAAGCCGATTAGAA
TACGAGACCCCAATCAAGGGGGCAAAGATATCACAGAGGAAATCATGTCGGGAGTAGGCAGTCGGAACTCCACCCCGCCCATCCTCAGACCCAACTCTAC
GCCGACGCCCCCTCAGCTGCCTAGCCAGGCGCCCGAGCACGGCCCAGTGGTGTACGGGACTATGGAGAGTCCTCATCTCCCAGCGACTTTCCCTGCCGCT
CCTGTCAGCGAACCCAAACCAGAAGAGAAGCCCAAGCCGGAGCACACCGTAAAAGCCACGCCCCCCCCATGTGTGCGGCCCGACCCCGTAGATATTGGCA
CCAGTGATGTCATCGAGCAGACGCCCAGACCCGCCTCCTCTGAGCGCCCCGAGCTCCAACCGTGTCTTCTGCCGGGGGTAACGGCCGCCCCTATCGTGGT
ACCCATTGTGCCCACCGCCCAAGCCTTTCCTTGCCACCTGGATGATACGAGGGGCAGCGAGAGCCCGGCAGAGGTGCGTGCCCCCACTCCCAGCACCCCG
CAAGAGTCAGACCCGGCCGAAACGGAGGAGGTGGTATGCAAGGACCCTGTGGGGCAAGTGGCGCCCGCTGATGCCCCTCTGCCTGCCTCTGCTTTGCCTG
CTCAGGAAATGAATGGCCTAGGGGACGATATGGCCGGTACGGCGGCCGGCGCTGCAGGGGAGGAGTCGGCCCTGGCAGAAGTGGATTTGCCCGAGGTGCC
CGGTTTGGCTGCGCCAATGGATGCAGACGCTGAATGCACTACTTCTTCCAACGTGCCTCTTTCCTCCCCGGCTCTGTCTGCCTCCCCACTGCCCGACTCT
CCTCCTCCTCTCATTGCTGCTAGCCTCTCCCCCCCTGCCAGCGCCCTCGCCACCCTCTGTGCCCAAGATGTGGCAGAGCAGGAAGATGGCAGAGCCGCCA
CCCTAGAGAGTGACGGAGAGGGACAGGGCAAAGGGAGAACCGAGGGGGACTCCGAGCTGGAATCCAGTGTTAAACTCCTGAGCATCAACTCCAAGAAGAG
CCCTGTGGAAGCCCCGGCCGCCATAACTGCACCAAAGATGTGGCAGAAACCAAAAGATGAGAGTCCAGACATAGAGGAGATCTCACAGTCAGAGACCGAG
CCACAATATGAGGATGATCCCCTAGAGGACAAAGTCACAGAATTGGATCAAGACCCAGTCACATACAGCGGTACCAGCACAATGGAGGTGGAGCAGCTGT
ACCTTAACAATGCCAAGCCACAGGAGCAGAATGGGGAGGTGGAGCCTATACGCAATGGGGCTGATGCCGTCTCTGAGAGTGAAGGCACCGATATCCCATC
AGGCACTGAGGAAGGCTTGGCCTACCCTTACAGTAAAGCAGGAATCGGTGCAGCCGAGCAACCCCCCGACCCCTCTCAGGAGCCCCCGGCACCTCCACAG
TCGGCTGATGGGAAGCGCCAGTACGACCGTGACTTCCTGCTGGGGTTCCAGTACATGCCCGCGTGTGTCCAGAAGCCCGAGGGCCTCCCCCCCATCAGCG
ACGTGGTTCTGGACAAGATCAATCAGCCCAAACTGCCCATTCGGGCCCTGGATGCTCGGATGCTGCAGCGGGGGCCAGATTTCACCCCTGCCTATGCTGA
TTTTGGGAGACCCGCCCCTGGGGGCCGGGGCATGCCGCTGCTGAGTGTGGGCCCCCGGCGCTCCCAGCCGGGACAGAGGCGAGAGCCCCGAAAGATCATC
ATGAACATCTCCGTCAGCGACGACGTCCATCTGAAGAAATCGGAAAACGCCTGGAAGCCCAGCGTCAAACGGGATAGTCTGCCGGAGGATCCGGATAGCA
TCAGAACTCAGGCCCTGTTCCGCAAGGTCCGGAGCATTCTCAACAAGCTCACCCCTCAGATGTTCAACCAACTGATGAAACAGGTGACGGATCTGACGGT
GGACACAGAGGAGAGGCTCAAAGGGGTCATTGACTTGGTCTTTGAAAAAGCAATAGATGAGCCCAGTTTCTCCGTGGCCTATGCCAACATGTGCCGCTGC
CTTGCCACGCTGAAAGTGCCGATGGCCGACAAGCCGGGGAGCACGGTGAATTTCCGTAAACTGCTGCTCAACCGCTGCCAGAAGGAGTTTGAGAAAGATA
AAGCTGACGACGATGTTTTCGAGAAGAAGCAAAAAGATCTAGAACTGGCAACAACGCCAGAGGAGAAGTCTCGGCTACAGGACGAACTGGAAGAGTCTAA
AGACAAAGCCCGGCGCCGGTCCATTGGGAACATCAAGTTTATCGGAGAACTATTCAAGCTGAAGATGCTGACAGAAGCCATTATGCACGACTGTGTGGTC
AAGCTGCTTAAGAACCATGATGAGGAATCCCTTGAGTGCTTGTGCCGGCTACTCACCACCATCGGCAAGGATCTGGACTTTGAGAAAGCCAAACCACGTA
TGGATCAGTACTTTAACCAGATGGAGAAGATCGTTAAGGAGAGAAAAACCTCATCTAGAATCCGCTTTATGCTGCAGGATGTCATAGACCTCCGGCTGAG
TAACTGGGTGTCCCGCCGGGCAGACCAGGGGCCCAAGACCATCGAGCAGATCCATAAAGAGGCCAAGATAGAGGAACAGGAGGAGCAGAGGAAAGTGCAG
CAGTTAATGACCAAAGAGAAGAGGAGACCAGGGGTGCAGAGAGTGGATGAAGGCGGATGGAACACTGTGCAGGGGGCAAAGAATAACAGGGTGCTGGACC
CTTCCAAGTTTCTGAAAATCACTAAGCCCACTATAGATGAGAAGATCCAGTTGGTTCCCAAAGCTCAGCCTGGCAGCTGGTTAAAGGGGAGCAGCGGAGG
TGCCAAGGCTAGTGAGACTGAATCTCTGAGACCGGGAGCCCCCAGCCTGAACAGGTTCTCTGCTCTGCAGCAGCCGCTCTCCTCTGTGTCTTCCTCCTCC
TCCTCATCGTCCCCCTCTGAGCTGGACTCTCGCAGGTTACTAACCAGCCGTGGAAGCACGGGGCGAGAGAAGAACGACAAACCCTCTGCGCCCTCCTCCA
TCCCGTCTGCGCCCCGACCCGGCACCCTCCTGCGCGGCAGCAGCAAAGACTCGGGCGACAATCAGGAAGAGCTGCGCCGGGAGACGCTCCAGACGGTCAA
ACAGCTGACGAACGTCGCCGAACCCGACAAGAACACTGTGCCAGAGAGACGGCCCAAAGAGCCAGCCAAGTCCGAGCCGTCGGAACCAGACAAACCCACC
CTGTCAGAGGAGGAGATGGAGAGGAAATCCAAGGCCATCATTGATGAGTTTCTCCATATTAATGACTACAAGGAGGCCATGCAGTGCGTCGAGGAGCTCG
GTGCCAATGGGCCGCTCTCAGTCTTCGTACGACAGGGGGTGGAGTCAACGCTGGAACGGAGTCAGATCACCAGGGATCACATGGGTCAGCTCCTCTACCA
GCTGGTCCAGTCTGGCAAACTCAGCAAACTGGACTTCTTCACTGGGTTCTCGGAAACGCTGGAGCTGGCCGACGACATGGCGATCGACATCCCGCACATC
TGGCTGTACCTGGCGGAACTGGTAACCCCCATGTTGAAAGAAGGCGGGATCTCTCTACGAGAGCTGATAACAGAGTTCGGCAAAGCTCTGCTCCCTGTGG
GACGCGGAGGGGAATTATTGTCTGAAATATTGCACCTTCTATGCAAACAAATGAGCCATAAGCGAGTAGCCGCGCTTTGGAGAGAGACCGGCCTTCGGTG
GAGAGACCTCTTACCTGAAGGAGAAGACGTCCAGAGCTTTGTCAGCGAGAAGGCCAGCCTAGATGAGAGCCAGATGAGTTCGCCCACATTCCTTAGAGCT
TTAATGACGGCGGTGTGCAAAGCGGCAATTTTAGGGGACTGTTCTTCCTGCCGCGTAGACATCACCTTCCTCAAGCAGAGGGTTCCGGTCCTGCTAAAGT
ATCTGGACTCGGACACAGAAAGAGAACTACAAGCACTTTATGCACTACAAGCATTGATAGTAAAACTTGATCAGCCTCCCAATGTGTTGCGCATGTTTTT
CGATTGCCTCTATGACGAGGAGGTGATTTCGGAGGACGCTTTCTATAAGTGGGAGAGCAGTAAGGACCCGGCAGAACAAGAGGGCAAGGGGGTAGCACTA
AAGTCGGTCACAGCCTTCTTCACTTGGCTCCAGGAGGCAGAGGAGGAGTCGGAGGACAATTAGGAAGTTCCATCCAATGAGAGCCCGTCGCTTGCCCCCC
GGAGACGCCGCCAACGCCGAGCAGTTTCAGCCTCTTTATATCTTTAGCTTTTGGGTTTTTCTCTCTTTTGTTCCAGTTTTGCCAGTCAGGGTACAGCCAA
TTGGATTCCCACAGGGGGGGTGGGCGTGGCTATTGGGAAAGGTGCCCCAACCAGTGTTAAGATGGGGGAGATGTGACCCCCCAAACGGCCGAGGGGGGAC
TGACCCCCTTTCCCACATTCTATTTATTAGAGTCGATATTTTCTGTTTTAAAACTTCCCATTTTATTTTAAAGAACCCCCAAACCCAGAGACTCGCCCCA
AAACTGCCGATCATTGGAAAGAGAAATAATTTATTCCTTTACAGACGGGTGGCGCCAGTGACCCACTAACCCCCTAACGTAGGAACTGGGGGGGCAGGAA
ACCCCCTCCCTTGCTGTGTAGCCTGCCCTCTCCCCTTTTGGGGGTCCCTGCCCATTTTAGTGTCACATTTTGGGGGGCAGCGGCTACGGACTGGGCTGCA
GGTTCACCCGCAGTCTCATTTGTGGGCACTGCTGTAAAGCTGAGCAGCCGGGGAGCCTGCAGATGTACCCCAATGTTGGAGGGGGCCCCCTGCCCTGCAC
TCTGTCGCCCCCTGCCATTGCCCCGGGTATGTACAGATGAGGGGGGAGAATTAGACTCGCGGGGGGGGGTTCCCAGGTGTAGCCCACCTTCAGCGCTGCA
TTCAAGGGAGGGGGGCAAATGGACTTCCAATCTCGCTGTGGCCCTAACGCGCAGAATAAAGAGCTGGAACTGGCTACGGACTGGCACAGAGCGGGCACCA
CAGCGGGCACCTTGGGACTGACGGTGTCTCGGAGAAGCTGCCGGAGCTTTCCAAGCAATTGGCAAACGAGACTGTGAGATGCTTTTCTCTTACATTGTAT
TTCTATTCATTTTAGCCTGGTTTCCTATGGAAAAGAAGGCTAATAAAGTAACCTGTACCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX902658 [+]ccgagggtccgtcctgccggactgctggaggcggacgcagcgccatgttggatgctccgctttctgagtgaggaaaatccgccggctctgagcatccccg
aagcgacagcgacagccgcccccccgggccgtgtccctcccccatgaggatcgggtcttgagccccctgctgcctccctattgcccctgtgccctggggg
gccccgctctgccatgagtctcccgcagaaagcggccctcaagcccgccgcaccgaccggcaccggagccctagtggttgggcctccccccagtgccgcc
cctcccgccgcagccccctccgccatccgggcccttcaaccgccgcctcaaatatccaggggagccctgcaacagagcctcgatgagcgaatcttctccc
cctccgccgtttctgccgtctacagcacagttgcagcacaggtagccaggcaacccggccctccggccccttctccttattctactcatgatttatccaa
gggacaccctaatctggctgccacccctccagggcatgccgcctccccaggattgtcacaggcttcctatgcgtcgggccagagcccagcagcacccacc
ctggtgtaccctcagccaccgcagactatgagtactcagccccaaaatcgctcaccatttgcagctggaccccgacctgcccatcaccagtttttccaga
ggactcagattcagcctccccgtgccgccatccagactagtag
3' EST: CX902657 [+]agaaaaaagcctcccccagccccgtttgccaattgctgggaaagctccggcagtttctccaaaacaccgtcagtcccaaggggccccctggggggccccc
tctgggccagcccgaacccagttccagctctttattctgcgcgttagggcccccgcgagatgggaagcccatttgccccccccccttgaaggcagcccta
aaggggggctaccccgggaaacccccccccgcgaggcaaattccccccccccatcggaacaaacccggggcaatggcagggggcaacaaaatgcagggca
gggggccccccccaacatgggggaacattggcgggccccccgggggctcagttttacaggagggcccacaaaagaaactgggggggaacctgcagcccag
tccgaagcccctgccccccaaaaggggacactaaaaggggcggggacccccaaaaggggagagggcgggctccaccgcaggggggggggtttccggcccc
cccagttcctacgttagggggttagggggccactggccccccccgtctgaaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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