XB-CLONE-3597772
Clone Name: IMAGE:7684739
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Gene:
igsf3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127336 [+]
CTGGCTCTCCGGTACCCGGGCTGGAACACGGAGATTTGCCCACGGAACCCCTGGCACCTATGGACCTGTAACTGGCACAGCACCAAGCGCATCATGGGCA
CGGCTGCCTTACTGATCCTCCTGGCAGGTGTGAGCTGGGCCCAGCGGGAAGTGGCCATACAGCCGGGGCCCCTCTACCGGGCAGAAGGCTCTCAGATCAG
TATCTGGTGCAATGTGCGTGGCTACCAGGGGCCCTCGGAGCAGAACTTCCTGTGGTCCATATACCTGCCCACCGCCCCTGAGAAGGAGATCCAGATAGTC
GGAACCAATGACCCTTCCTTCTCCTACGCCATCTACTCACAGCGAGTGCGCTCCGGGGGCATCTACGTGGAGCGAGTGAGCGGGGACCGGGCCCTCTTAC
ATATCCGCCAACTGCAGGACCAGGACACGGGCGAGTACGAATGCCACACCCCCAACACTGACCCCTCCTATCATGGCAGCTACAGCGCCAAGGTCAACCT
GCAAGTGATTCCGGACACTCTGACGGTCTCGTCCCCGGCACAGACGATCCAAAAGGTGGAGGGGGGGTCTCTGCAGGTGACCTGTGAAGTTTCCCAGAAT
TCCTCCCGGCACACCCACCTGTCAGTCTCCTGGCTCCGACTGTCTGAGGGGGAGGCGACCGAGATCATCTCTGTGACCCAGAACTTCTCTGTGCGGCCCG
GTCCCTCCTACGCCCAGAAGCACTCGGTGGGGGACGTCAGGCTGGACAAAGTGGGCGACAGCACCTTCCGCCTGACCCTGTATAACCTGCACCCCAGCGA
CCAGGGCGACATTTACTGCGAGGGCACGGAATGGATCCAGGATCCGGATGGGACTTGGTTCCCCCTGACCCAGAAACGCTCAGAAGGGACGTCAGTGACG
GTACTGCCAACAGACAAGGAATTCAACGTTCGCTTGGAGACAGAGCGTCGGACGTACAATGGCGGCGACACGGCGACTCTCCGGTGCATCATTGAGGCGC
AGAACCCGGGGGATCGCTTATTCGCGGTGTCGTGGGCATTTAACAGCTCCCTGATTGCCAGCCAGAGCCCCGGGGGGGTCCCATCCCTGACGGGGGAGTA
TGCCAGGCGGGAGGATGGCGGGGAGCTGCGGGTGGGCAAGGACAGCGACAGCGTCTTCTCACTCAAGATATTTCGCCTTCGCCCCGAGGACAGCGGGAAA
TACAACTGCCGGGTAACGGAGCGCGAGCGGGGCCCCTCCGGGGAACTCATCGACCGGGAGAGCAAGAGGCCCAAGAACATCCCCATCTCTGTACTGCCCC
TGAGGACGTCTCTGACAGTCACGGTGACAGCCAACTCCAGCTCGGTGCTAGAGGGGGTGCGCCTGTCTCTCAGCTGCTCGGTGGCATCTCTCGCCGGACC
CCAGAGCCGCGTGTCGGCCTCCTGGCACCTACAGGACAAACAGGGGCGCCAGAGGGACGTGGTGCGGCAGGACCGGGATGGAGTAACGTGGGCCGGGGAG
CAGTACCGGGAGAGGCTGGGCACCGGGGGGCTGCGGCTGCTCCGGTCCGGCTCGGATACGTTCAGCCTGGAGCTGGAGGGCAGCCAGAGAACGGACGCGG
GTTCCTACGAGTGCCGGGTAGCGGAGTGGGTACCGGCCCCTGACGGAGAGTGGCAGCTGCTGGGCGAGAGATCAGCTCAGGCCAACGTGGATATCCTGGC
ACTGGAGACCGGATTCGCCGTGACAGCAATCACCCGCACGCCCGGCGTAACCTACTTCGACTCATTCGACCTGCAGTGCATCCTGAAGCCCCACTATCCC
CCCTGGGTGGGGGTGTCGGTCACCTGGCGCTTTCAGCCCGCGGGGGGCGGAGACACGCACGACCTGGTGACCTTCAGTCGCTCCGGCGGGGTCCAATGGG
GGGAAAGGGCCGGAAGTTTCCGTGGCCGGAGTATTGTGGAGAAGGGAGACTCCACTCACACTGTGAGGCTGAGTGTGAGTCGGGCCAGTGATTCGGAGGC
GGGCAAGTACCAGTGTGTGGCCGAGCTATGGAGGCGGGAAACCAGCGGCATGTGGGCGCGGCTAGCTGAGAGGGCATCCAACCTGCTGGAGATCAGAGTC
CAGCGCCCAGTGCCGCGGCTGCAGGTCACTAAGGTGACGAGGACCCTCAGTGTAGTGGAGGGGTCTCCGGTGACGCTGAGCTGCTCCATTCGCTCCCAGA
GTGGGCCGGACTCGCGCTTTGCCGTGCTGTGGTTGGTGCAGCAGCCCTCGGGGGCGGAAGGGAAGCTGATTGGGCGCAGTGACGCGGGCACAGAGTTTGG
CACCTACCCCGAGGGGGCGTTGCTGAAAGGGCGGCTGCAGTTGGGGGTCACGGCACCAGGGCAATACGCTCTCACCCTGCAGGGGGCACGTACCGACGAC
AGTGGCAGCTACTACTGTCAGGTGGAGGAGTGGGCAATGGACCCCAACCAGGCCTGGTACCGATTGGCTGAGGAGACGTCGGGGCTGACTGAGATCCGTG
TGAGACCACCAGATGCCAACTTGCAGCTGGATCAGTTGCCCCGGAACGTATCGGCCCTGGAGGGGCAGAGTGTGACCGTTACCTGCCACGTGCTGAACCG
GACCCTCCCCGACTCCCGCCTGTCCCTGGAGTGGCTGTGCTGGCGGGCGGGGCATATGGAGAGGCGGGGCCTGGCCAGGCTGACGGTGGATGGGGTACTG
GGCTCAGTAGAGGAGGAGCCGGGCGATGTGGCCCCGGGGCTGCCCAGTCGCGTGCAGGTATCTCAGCCGTCCCTCGGGCTCTATGCCCTCACCCTGCGTG
GCACCGAGGTGCGGGACAGTGGCACCTACAGCTGCCTGGTACAGGAGTGGCTCCGGGACCCGCGGGGGCAGTGGTACAGGCGCACTGAGGAGCGATCGGG
GGCCACTTATGTGAGTGTGCGCCAACCGGACCCGCAGCTGCAGTTGGACCCCTCTCTGCTCAACGTGTCGGTGATGGAGGGCGGCCATTTTGATCTGGAC
TGCATGGTATCGTCGCGCTCGCGGCCGGACTCCCAGCTCGCCATTTCCTGGTCCCTACAAGGCACATCCCGGGGGGCGGAGCCGGAGACGCTGCTGAATG
TGGACCGTTCCGGGGTTTGGGCCCCGCTGGCGCCGCGGTGGGAGGGGCGCTTACAGCAGCTTCAGTTGTCGCCTACTCTGTTCCGCCTTCACGTGCCGCG
CGTCGGCCCAGGGGATTCTGGGAATTACACCTGCCTGGTACAGGAGTGGCTGCTGGGACCTCGGGGCGACTGGTACCTCCTGGCCCAGGATGAAGCTGTG
ATTGGTTGGATCAGAGTGCAGAGAAAGGAGTCCAACCTGCAGTCGACCATCTGTGCCAACGATGCCCTCTTCTACCTGGTCTTCTTCTACCCCTTCCCCA
TCTTCGGCATCCTCATCATCACCATCCTGTTGGTGCGCTTCCGCCACCGGCCCACGGGCAAGCCTGGCGAGGGCAAGAACGGGGTCCCCCTGCTGTGGAT
AAAGGAGCCCCATCTCAACTACTCCCCAACGTGCCTGGAGCCCCCCGTGCTCAGCATTCACCCGGGCACCATAGACTAAGGAGCCGGGCAGAGGTTGTAC
CCGCTGTGCCCGTCATTGCCCCCCAGAGGGAGGATTCTGGAATCTGCTTTGGGGGTAACACTGCCGGGGCCCCGTGGGCCCTATTGTTGTACCTTTGTGT
CCTGTGTGGGGGCAGTCGGACTTGCCCCGCTGGTGCCAGGGGAGACTCAATTGGTTCCGGTTCTGGAGCTTTAGCTCCGCCCTGTTTGGAGACTCTGACC
GGCCCTGCGGGTCGGAGGTCGGGCCCTTCAGCTGCAGGAAATGACTTGGGGCCCCTGAGTGGAATTGTGGGACTTTGTATCGTTGGCAGGGAGGGGCATT
TGTGGCCCTCGGGTTTCTGTGTCCTGGGGCCAAACGTGGGGGCCGCGACCCATTTGTGTATAATATTTAACCCTTTCCTCGCTGAGTTGCTGCTTCCTGT
GAAGTGAAAGTGTCTTTTATATCCCTCTGAGCCAGACCCGCCCCCTTTTTATCTGCCCCACTTTGCCCCGCCCCCAGTATTTCTGTATATATTTTATGCC
TCTCTTGCACTGCGGGGGTAACGCCCTGGCTGGCGTCTGCCTTTCTGCCCCTCCCCTATTGGCCCAAGTCTTCCCCTGCTCAGTGGGGGCTCTCGGGGGC
CCCTATCGGTGCTATTTCACCAGTTTTTGGGCAAAAGGAACATTCTCAGTCGCGCGTCTTCCTGCCCCCGGGGTCCCAGTTACCTCCTGCCCCCGGGGTC
CCAGTTACCTCCTGCCCCCGGGGTCCCAGTTACCTCCTGCCCCAGAGGCACCACCGAATGTAAAGCCCCTGACTTGCCACTGGCCGGGAGTGTACAGGGG
GCATTTCTGGCCTTTATGGTTGGTTAAGGGGTTAAGTATTTATACTGTCCCCCCCCCCCCGTAACCTGCCCTGTGCATGAGACCCCCCAATACCCCCAAT
CCCCCCACTTTGTGATATGTTGCACTGAGCTTGTTTTATATGCTGCCTCTTCTACAGTAAAGGTTCTGCCCCCCGAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA
5' EST: CX903375 [+]ctggctctccggctacccgggctggaacacggagatttgcccacggaacccctggcacctatggacctgtaactggcacagcaccaagcgcatcatgggc
acggctgccttactgatcctcctggcaggtgtgagctgggcccagcgggaagtggccatacagccggggcccctctaccgggcagaaggctctcagatca
gtatctggtgcaatgtgcgtggctaccaggggccctcggagcagaacttcctgtggtccatatacctgcccaccgcccctgagaaggagatccagatagt
cggaaccaatgacccttccttctcctacgccatctactcacagcgagtgcgctccgggggcatctacgtggagcgagtgagcggggaccgggccctctta
catatccgccaactgcaggaccaggacacgggcgagtacgaatgccacacccccaacactgacccctcctatcatggcagctacagcgccaaggtcaacc
tgcaagtgattccggacactctgacggtctcgtccccggcacagacgatccaaaaggtggagggggggtctctgcaggtgacctgtgaagtttcccagaa
ttcctcccggcacacccacctgtcagtctcctggctccgactgtctgagggggaggcgaccgagatcatctctgtgacccagaacttctctgtgcggccc
ggtccctcctacgcccagaagcactcggtgggggacgtcaggctggacaaagtgggcgacagcaccttccgcctgaccctgtataacctgcaccccagcg
accagggcgacatttactgcgagggcacgg
3' EST: CX903374 [+]cggggggcagaacctttactgtagaagaggcagcatataaaacaagctcagtgcaacatatcacaaagtggggggattgggggtattggggggtctcatg
cacagggcaggttacggggggggggggacagtataaatacttaaccccttaaccaaccataaaggccaaaaatgccccctgtacactcccggccaggggc
aagtcaggggctttacattcgggggggcctctggggcaggaggtaactgggaccccgggggcaggaggtaactgggaccccgggggcaggaggtaactgg
gaccccgggggcaggaaaacccccgactgaaaatgttccttttgcccaaaaactggtgaaatagcaccgataggggcccccgagagcccccactgagcag
gggaaaacttgggccaataggggaggggcaaaaaggcaaaccccagccagggcgttacccccgcagtgcaagagaggcataaaatatatacagaaatact
gggggcggggcaaagtggggcaaataaaaagggggcgggtctggctcaaagggatataaaagacactttcacttcacaggaagcagcaactcagcgagga
aagggttaaatattatacacaaatgggtcgcggcccccacgtttggccccaggacac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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