XB-CLONE-3597677
Clone Name: IMAGE:7684513
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Gene:
ankfy1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125724 [+]
GGCATTCCCGGTTTTTTAGAAGTGAAACTCAGTATTTGCTCTGCTGTTTGGAGTCCTTGTTTCAGCCTATACCGGTTTCCTTCCCCTACGTCACAGTTGT
CGGCCCCTCTTCTCTCGCGATATCAGCCGGGCGGATCCATCATGGCGGAAGAGGAGGTGGGAAAGCTGCAGAAGCATCTAGCGCTTCTCCGTCAGGAGTA
TGTCAAGTTGCAGCAAAAGCTGGCAGAGACTGAGAAAAAATGCACTCTCCTAGCAGCCCAGGCTAACAAAGAAGCCTCCAGCGACTCTTTCATCAGCCGC
CTCCTCTCTATCGTGGCACAACTCTTTCAGCAGGAGCAGTATAGTGATCTGATGATAAAAGTTGGAGACAAACACATAAATGCCCATAAATTTGTTCTTG
CTGCAAGAAGTGAGGTTTGGAGTCCAGCCAATTTGGCTTCAACTAAAGAACTTGACCTGTCAGACTCTAATCCTGAGGTAACTATGGCTATGCTTCGTTG
GGTCTACACAGATGAGCTGGATTTAAGGGAAGATGATATATTCTTAACTGAGTTGATGAAGCTAGCTAATCGTTTCCAACTTCAACTACTTAGGGAAAGG
TGTGAAAAGGGAGTTATGTCTCTGGTGAATGTGAGGAACTGCATACGTTTTTATCAGACTGCTGAGGAGCTAAATGCCAATGCTTTGTTAAACTACTGTG
CTGAGATTATTGCAAGTCACTGGGATGACCTAAGAAAAGAGGACTTCAGCTCAATGAGTGCTCAGCTACTATATAAAATGATTAAATCAAAGACACAGTT
CCCTTTGCACAAAGCAATAAAGTTGGAAAGAGAAGATGTAGTTTTCTTGTACCTAATAGAAATGGATTCTCAGTTGCCTGGAAAACTAAATGAACTGGAC
CAAAATGGAGACCTTTCCTTGGATTTAGCTCTTTCCCGGAGACTGGAAAGCATAGCAACTACATTGGTTAATAACAAAGCTGATGTTGACATGATGGACA
AAAAGGGTTGCAGTTTATTACATAAAGCCATTTGTAGAGACGATAAATTTGCTGCAAACTTTCTAATAAAAAATGGTGCTCTTGTAAATGCTGCCACTGT
TGGAGAGCAACAGACTCCTCTGCATTTGGTAGCTTCTTGCACAGTAAAGGACCGGTCTGTGGAAGTGATGTCCGATATAGCACAAATTGCTGAAGCACTT
TTAAAAGCAGGAGCAAATCCTAACATGCAAGACAGCAAAGGAAGGAGTCCTCTGCACGCTGCTGTTCTTGCTAGGAATGAGTATGTCTTCAACCAACTCC
TTCTGTGCAAACAATTAGATCTAGAGCTGAAGGATCATGAGGGAAGCACAGCTCTGTGGCTTGCTGTTCAGTATATCACAGTGTCTTCTGACCAGTCTGT
AAATCCTTTTGATGATGTCCCTGTTGTAAATGGCACCTCATTTGATGAAAACAGCTTTGCTGCCAGACTTATTAAACAGGGCAGCAACACAGATGCACCA
GACACTGTAACAGGCAACTGCTTGTTACAAAGAGCTGCTGCTGCAGGGAATGAAGCTGCTTCCCTGTTCTTGGCAACCCATGGGGCAAAAGTCAATCATA
AAAATAAATGGGGTGAATCGCCTTTACATACTGCATGTCGGCATGGACTTTCTAACCTTACAGCAGAGCTTTTACAACAAGGAGCCAACCCAAATATTCA
GACTCAAGAACCTATTACTTCTCCAAGAGGACCTCCAGCAAACAGTCTGGCAGACAGCACTTACTTACAAACACCTCTGCACATGGCAATCGCATATAAC
CATCCTGATGTTGTTTCTGTCATATTGGAACAAAAAGCTAATGCTCTTCATGCAACAAATAATCTACAGATCATACCTGACTTTAGTCTCAAGGACTCCA
GAGAACAGACAGTGTTAGGACTGGCACTATGGACAGGCATGCACACCATTGCCGCACAACTCCTGGGCTCTGGAGCATCCATCAATGATACAATGTCAGA
TGGACAGACACTTCTACACATGGCAATACAAAGACAAGACAGCAAGAGTGCCCTGTTCCTGCTAGAACACCAAGCTGATATCAATGTCCGAACCCGGGAT
GGCGAGACAGCACTTCAGCTTGCCATTAAAAATCAGCTTCCTTTGGTGGTGGATGCCATTTGTACAAGAGGCGCTGATATGTCTGTTACTGATGAACAAG
GGAATCCCCCTCTGTGGCTTGCACTAGAGAATGAGCAGGAGGATATTGCCTCAACTCTGGTAAGACATGGATGTGATGCTACATTCTGGGGGCCAGGGCC
AAGTGGATGCCAACAGACGCTACTTCATAGAGCAATTGATGAAAACAAAGAGCAAATTGCTTGTTTCATTATTCGCAGTGGCTGTGATGTAAATAGCACT
AGAAAGCCTGGACCAAATGGAGAAGGAGAAGAAGAGGCCAGAGATGGGCAAACCCCCTTGCATTTAGCAGCTTCTTGGGGACTGGAGGAGGTAGTGCAGT
GCCTTTTGGAGTTTGGTGCCAATGTAAATACTCAGGATGCAGAGGGGAGAACTCCAATACACGTTGCTATAAGCAACCAGCATCGTGTCATTATCCAGTT
ATTGATCTCACACCCTGACATCCGGCTGAATGTTCGTGACAGGCAGGGTATGACCCCATTCGCATGTGCCATGACTTTCAAAAATAACAAGGCCGCCGAG
GCAATTCTGAAACGGGAACCAGGAGCCGCTGAGCAGGTGGATAACAAAGGGAGGAATTTCCTGCATGTTGCAGTTCAGAATTCAGATATTGAGAGTGTCT
TGTTCTTGATAAGTGTTCAGGCTAACGTTAATTCAAGAGTCCAAGATGCCTCCAAGCTAACCCCACTGCATCTAGCTGTGCAAGCAGGATCAGAGATTAT
TGTCCGGAACCTGCTTCTTGCAGGTGCAAAGGTGAATGAACTTACCAAGCATCGTCAGACTGCCCTTCACCTAGCAGCACAACAGGACCTTCCAGTTATT
TGTTCTGTTCTCCTTGAGAATGGTGTTGACTTTAGTGCTGCAGATGAGAATGGAAATAATGCTCTGCATCTGGCAATAATGCATGGGCGCTTGAACAATA
TACGCTGCCTTTTAACTGAATGCAATGTGGATGCAGAGGCTTTAAACTTAAGAGGTCAGTCCCCAATGCATATTTTGGGGCAATATGGAAAGGAAAATGC
TGCTGCCATCTCTGAACTCTTTCTGGAATGTATGCCACAGTATCCTCTTGATAAACCAGATGCAGAAGGAAACACTGTGCTGCTACTAGCGTATATGAAG
GGAAATGCAAATCTTTGCCGGGCTATTGTGAAAGCTGGTGCCAGGCTTGGAGTAAATAACAGCCAAGGAATCAATATATTTAACTATCAGGTTGCAACCA
AGCAGCTTCTTTTTAGACTTTTAGATATGCTATCTCAGGAACCACCTTGGTGTGATGGCTCAAATTGTTATGAGTGTGCTGCAAAGTTTGGAGTCACAAC
CAGAAAACACCACTGCCGCCATTGTGGACGTCTGCTCTGCTCCAAGTGCTCAACCAAGGAGATTCCCATTATAAAGTTTGATCTGAACAAACCTGTTCGG
GTTTGTGACATCTGCTTTGATGTGCTGACTCTGGGTGGAGTTTCTTAGTACACGAGGGAGAAGATACCTGGTTTTCTTAAAGATGGCAACTCTGTCAAGC
AAGTGGACAACTGAGCAGCGGCAGTTTGCTCTTCCACCATGGACAGAATGGACCACATTATAATATATTCCAGTATATAAAGATTTGTATGATTGTACAT
GTATAGGCTTTGCTAGATGCTAACTGGACTTAGCTTTTTTTTTAAGTCAAGTAATAAATGTGTACCAGTGCTGAAAGTTTAATACTGACCCAATATCTTG
GGCTGGTCAGATTATCAAAATGATATCAGTGCTTACTTAAAGAAAATGTCTTTCTAATGAATTCTCCAGGATCAACTAATTCATGTTGTTACTCTTGTCC
ATTATCTCTGTAAAATGTGTAATTAGTGACCCTAAAATCTGGGAAGATAGTATGCTTGGAGAATTTTTTTAAATCTATAACTATATATACAGGGTTCAGT
TGCATATAAAGCCAATTAAACTGTGGCCTTAAACTATAAGCTTTATAACCACCACTCTAATTAAACCATTGTAGCACTCCAGTTATTTTGATAAACTTTC
TATGTAAGACCCTTAGTCTTTGTTTAATTGTGGCTTCCACAAAGTACTTACCAGCTGATGGAAGGTCCTTTAAGTTTTGCATATCTGCCTTTACAGCTTA
TTTCCCTTTTTCTTCTGTATTTGATGTTTTCTTTGCTTCTTACTAATCGCGTCCCTAAAATAATATGAAAAGCTCTATGTAATAGAATCAAGTTAAAGAT
GTGAAACTGTTCAAACACTAATGTGTCATACAAAACTAAATGTTGAGCCTAAACCTTTGATATACAAATTAAAATATCTAAGCTCCAAGGAAAAAAAAAA
AAAAAAAAAAAAAAAAAAA
5' EST: CX903463 [+]gcattcccggcttttttagaagtgaaactcagtatttgctctgctgtttggagtccttgtttcagcctataccggtttccttcccctacgtcacagttgt
cggcccctcttctctcgcgatatcagccgggcggatccatcatggcggaagaggaggtgggaaagctgcagaagcatctagcgcttctccgtcaggagta
tgtcaagttgcagcaaaagctggcagagactgagaaaaaatgcactctcctagcagcccaggctaacaaagaagcctccagcgactctttcatcagccgc
ctcctctctatcgtggcacaactctttcagcaggagcagtatagtgatctgatgataaaagttggagacaaacacataaatgcccataaatttgttcttg
ctgcaagaagtgaggtttggagtccagccaatttggcttcaactaaagaacttgacctgtcagactctaatcctgaggtaactatggctatgcttcgttg
ggtctacacagatgagctggatttaagggaagatgatatattcttaactgagttgatgaagctagctaatcgtttccaacttcaactacttagggaaagg
tgtgaaaagggagttatgtctctggtgaatgtgaggaactgcatacgtttttatcagactgctgaggagctaaatgccaatgctttgttaaactactgtg
ctgagattattgcaagtcactgggatgacctaa
3' EST: CX903462 [+]cccctggagcttagatatttcaatttgtatatcaaaggtttaggctcaacatttagttttgtatgacacattagtgtttgaacagtttcacatctttaac
ttgattctattacatagagcttttcatattattttagggacgcgattagtaagaagcaaagaaaacatcaaatacagaagaaaaagggaaataagctgta
aaggcagatatgcaaaacttaaaggaccttccatcagctggtaagtactttgtggaagccacaattaaacaaagactaagggtcttacatagaaagttta
tcaaaataactggagtgctacaatggtttaattagagtggtggttataaagcttatagtttaaggccacagtttaattggctttatatgcaactgaaccc
tgtatatatagttatagatttaaaaaaattctccaagcatactatcttcccagattttagggtcactaattacacattttacagagataatggacaagag
taacaacatgaattagttgatcctggagaattcattagaaagacattttctttaagtaagcactgatatcattttgataatctgac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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