XB-CLONE-3596488
Clone Name: IMAGE:7685261
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Gene:
nhsl1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168125 [+]
ctgacacgaactactgggagattttgtctgcaggatctgaaccactggatgtttaaatggcgttgcgggacttttgaacaacttatacaggacttcgggc
tttgttatgaaaatcgcgactgtttcgcgactgtttcgcgacaagtgggacttttgcgcggcatggagacgtatggtcctttgaaaccacagtgcactgc
gtctaaaggaaatagaattaagttgcagtaatacagttgagaacaggtgccatgccatttcacctcaggctagtgcaaccaatttatgtgtgccgactgg
gtgacccagatgcccaagcgcccagaggaggcaattatttgatctttaactcattggaggatgttagctcatactcgctgcggcaaatcgtccgtcaact
ttccgatctctgcagacatgctgagaacattttttccagtattcattttgaggcgtcctctttgggggaccgaactacccgcttgaagcacagaatttcg
tttctacaacataatattagccacctggaccataaaagggttacagtgccggtttccaatctagatgaagaaagtaaatggacagtccattacacagcct
cttgtcatcaacaagagaatgtgttcctctcatcatctagacctttatgtgtagatgaccttcacaggcaagctaaagtgaaccttaaaactgtgcttag
agaatgtgacaagcttcggaaggatggctgtcgcagttcacagtactactcccagggaccaacattctcgacactcagactggattgcacaagctatcag
gaagaggaaaatacacatgagtcaaagttttctgttgcatttaatgaagaggaacagatcctttcaaccaaaaggcccgagactcctgtgttgcttgagt
tttctgaaacgctcactaactggaccaagtcactgccactgcctactccagaggaaaagatgcgtcaggaggctcaggcagtacagacagatgtaattcc
tataaacataacaggggagaatttcaaccgccaggccagctttcagcggtcacttatttatacagacactgccgtaagatgttcgaagaaagtcaaaagg
agaaagactataacaggcgtcccaaacaacttacagaaggagctagcaggtactggacactctgacctaagaaggaattcagtgctcatacctgatgatt
ttaatactttggaacattgtcaccactcaatgcaatttctggacaccagagattttggctgccaaaccaataaaataaaaatagtgccaccatcaattag
aagaatcagagcacagaaagggcaaggaattggaattctgatgtccacttcctctggcaacatgtctactctaagtgactctgcagcttgttcttcatat
ctaaatggtaacctgcacttccgtagtctgcccaggattggtgcaagagaatctcttcaatcccttaaccaaaaaaattattattacagcaacatggagg
acccttttacaactttgccttggcagttaaacaaactgcaagtggatgatactaatgttcaattaaggagtaaccctaggtcaactattaatcaaaggcc
tgagtctcaagaagtaaaaaattctactagtgaaattgcctcaagtccagcatgtatggtctcgcctcattcttcttattcaacaaacattattccaaat
gccatactttcatattcctcagaagtaataagtattcacactccccacagaggtggacttctggacaagaaaccaagagtccacagttcatcttctaaca
gaaatggtattagcagggactatacttctggcattaaagataattcctcacctggtaactggcgtgaaaacagttcatctaggaactcacagatatcaga
tacaaagtcacctatcagtaaaaaaagagatgttccacacaacgttccttgcaaaattaacactggctttaacaactactcatttgacagtgacaatcaa
agtgaattaagctactcagatggtcgtcaaagaaatggtagtataactagcagcattaatagtgctgacaactggttgtatgaatctaggcacaatgacg
gtgctccatctaggaaactatcttctgtaatttctgggtctcctgttagcaatatgagcgcctgcagttctaaggggaaggtagattccagctctttgta
ttttctgaaccatgatgatttttgcacatcaatgcatatagattctggaattagtttgacggaccaaagcattcagaatctacactataatataaatatc
tttgaaacaaaaaaagctgcaaatatagaagtgaaaacaggatatagtgacaaatcacttccacgtagcatatctttaaagaaatctaaaaatcctcccc
ccccaccttccaggacagactcacttagaaggccatctaagaaagatattcagaccagtgggcaggtgctcagtgaaactcttattgctacgcttcaaca
gtccttgcaactaaacttgaagagtaagagcactgggtccactttgcagtcaagtcactgtatggattatgcagatccctgcatcatgtgctcatgtagc
cagagtacacttagcagcactagtagtggaaaatctgttattgctccaaatatgtacactctctgtactgtgacaccctctcagagtgaaaccagtagca
tgaaatctgaatatgcggatcagtggagttcctttattgactgttcacagactccaaaagagcaacccaaatcatttgcaaaacacaatctaagtaatcc
tgatggtcttaataattacagtgcatgtgagctgacctatggaatgaagaccatagcaccacatgggtacactggatcagttaaagttagtaattcctct
tcagaaaaaacatacagagttacatcaccatctagtggatattcaagtcagtcaaacactcccaccacattgacaccagtgcctcttttctcaaagaata
tatatccagaaaatggaaaatacaagttaaaaccaaaagtacctgaaagacgatcttcattggtatcctcggtttcagtttcatcttcctctacttccct
ttcttcaaatacattggactctctgcagcagaacatgcaaatttcaactgttatgttgtcagcccatcacactcttccaaccccacaactcactcctgac
atgcccccacccccagcaatgaatgcaaccaacacaatttttgtcgagtgttcaccaaaatgtccacctccttcccttgaatttacaaggctaacagcgg
atagcatggatatgtggcttcccttctctcaaaacactgccatgagtaaacctaaggacagtcttccatatccttcctttccaccttctctttccatgat
gcacagcatggtcccatcacctgtacctccacttaacattgaaatgctgaaaattgaaacagaatcatttcctctcagaaattctgaaagcagttatttt
gtacataatgatcacagtaaacatgaactgttgataccaactgttcctatagttacttctcatgcactagaaatggttcatttgagatctgtgaaaaaag
catcacaactggaaactaaaacattaagagaacacatgtcagaatcccacctacaggatgagacatccgccttattgatacagccatgtttagtaccagc
tgcttctccagaactaaattctagtgaaagccaaatagaaaatttcagcattcctgcccagcaagtctctggttttaaaagcaatgcagtgaaactaact
attggtgaaatagaagcagacaaggcatataaagagcacatagaaaaaaatacaaattcagcatacaatggaaactcatcagggcccaaattatctcctc
atgagaaaccaagtatgtcacaaagcacaccaaattcatctcttagcaaggagccgcctcttgtttcaaagaagcctaaacttgtacttattgccccaac
gccacaacttgatttggcagcagaaaaaatagctcaagtcactgaaaatgtgagaagcatgccaaatgcctttgaaaaagattcagttagcaaatgttgt
gtgggggagataaatgacagaactaaagatgaagacatagcaggtccaaccaatggtttggctcaccaagaacaagaagcctggttatccccatgcaaag
ctgctaaaaatatgattttcatactaccatcagttccagaaattcagaatcatcagcaaaaggaagagcagcaatccatgtcagatgaattcaacattag
tggtaacatttctgagactgagaatacaagctgcaaccagaatgcatctcaagatgatgagagtgctgaagtgtttaatacagagacggtgaattcagtt
ctctggacaagcagcgacattatgggaaatgtagaagagaccactccaacaaggcctgctacaactgaggatctttttgctcgtatccatagatccaaaa
aaaaaattcttggacgcaaagattctgacgatgatcgttgcagagatcactcgccttcttctccagttaccccaactggtaatatgcatctaagttcctt
caagcagacaggttcaatccaaagaagtgtcagaaaaactacaacaagtagtgacagtttcaaagcactgctgttaaaaaagggaagcaggtctgaaagt
ggcttccgcatgtctgctgcagaaatgctgaaacatactgatccaaggtttcagagatcaagatcagaatcatctctggagtttccagaaagccccacat
tgtcatcaccaaacaagaacaagaaggagcaacaggaatgggcaaaaagtgaagggctgatgcctagaagtatgtctggctctgttgtccggtgcagccg
cgccaagactcccccttctgcatctagtagcaaatacaatgtccgtagtcgtctccagagtagcccaatgactgttatatgtgaaggagaaggagagggt
tcagaaacagtggatagcagctgttcaaagggtcctcttttgttgagcacaaagagtctcgatatctgttacagtgctgataaagaagtgaccaaccaca
tactgcacataaactttaacacactattagaaaagagcacagatgttgctagtggctgcacaggggaaataatttaatactgaatgcctcattgtagctt
actgaaatgtataaagtaacggaagcactgggaatgccctagccctgaaaaagcaaggttggtcattgcttagcacttgttaactgaaggatgtgttatc
ttccatgtaagttagcatttggttgggcttggtttggtatgcagcaactgcacaataggaagtaaaagcagagatttaccagtgcatcagtagaaaactc
agtgttttatgaaccttaaacaacatttgctctagatctattaactcctatcagcttctcaaatatttgcagaccagaacatgtcttctgattcctccaa
ctagttgtagtggaacaaagtttgtgccagctatttttactttgagttgtcctaaacaataatatattcattgccttgaacatactgcttatagaatagt
ctttattccaaacatagttttttgcattggttgaaatttgagggttggtaatgggtttgtcagagtaacacaattgtgtgctatttactaatttttctgg
tacttttgttttttttttaataatttcttgtgtaaatttcagctgctgtgcagccatacaagcccagggttgatgcaaaaatactgattttcatattgta
agctcatagtgaacttctacttaagatttaatgaagttttctagtgagctgcaatttgagtgctgcatacaatccaaccattgcagtgtgtacgtttcta
ctaaagggaactttaggctgtgttactagtataagtgccttcaatacattgttttgcagtatttcatattgctttgaaatgtattgttgccttgaaagct
gctggatacacgtttgtctttcttcagcttgagctatgagccgtctttgtaactcttgcattgtttggcaattagatcacttgactgcaaaagaatgtgt
actagttgtcatgctaaataagcccattgtttttatcatctaaaaaagaaatgcaaaatttgagattaccaaagaaaacagcttttctaccttttaagca
atattaatgtaaaaatatttacttcttaaagttcaaatagcattccatgtcccttttattaactgaatgcatcatttaatgtactgttatctttataata
tttgtatatactgacattgcaaatctcacctttttacttgcattcaaagtttgtacattttttactctactaaaactctttttaactcatttattgctgg
agtttttgacagccataaatatgcaatgcagacctgcaatttatttatgtacgtttgttaaatgtggctgtgactctgtcctttctattactgtacacat
tcaggggcctttattttattagtactataataattctgtgattatgcttcaaataaagcaaggctgaaaataaaaaaaaaaaaaaaaaaaaaaaa
5' EST: CX904721 [+]ctgacacgaactactgggagattttgtctgcaggatctgaaccactggatgtttaaatggcgttgcgggacttttgaacaacttatacaggacttcgggc
tttgttatgaaaatcgcgactgtttcgcgactgtttcgcgacaagtgggacttttgcgcggcatggagacgtatggtcctttgaaaccacagtgcactgc
gtctaaaggaaatagaattaagttgcagtaatacagttgagaacaggtgccatgccatttcacctcaggctagtgcaaccaatttatgtgtgccgactgg
gtgacccagatgcccaagcgcccagaggaggcaattatttgatctttaactcattggaggatgttagctcatactcgctgcggcaaatcgtccgtcaact
ttccgatctctgcagacatgctgagaacattttttccagtattcattttgaggcgtcctctttgggggaccgaactacccgcttgaagcacagaatttcg
tttctacaacataatattagccacctggaccataaaagggttacagtgccggtttccaatctagatgaagaaagtaaatggacagtccattacacagcct
cttgtcatcaacaagagaatgtgttcctctcatcatctagacctttatgtgtagatgaccttcacaggcaagctaaagtgaaccttaaaactgtgcttag
agaatgtgacaagcttcggaaggatggctgtcgcagttcacagtactactcccagggaccaacattctcgacactcagactggattgcacaagctatcag
3' EST: CX904720 [+]attttcagccttgctttatttgaagcataatcacagaattattatagtactaataaaataaaggcccctgaatgtgtacagtaatagaaaggacagagtc
acagccacatttaacaaacgtacataaataaattgcaggtctgcattgcatatttatggctgtcaaaaactccagcaataaatgagttaaaaagagtttt
agtagagtaaaaaatgtacaaactttgaatgcaagtaaaaaggtgagatttgcaatgtcagtatatacaaatattataaagataacagtacattaaatga
tgcattcagttaataaaagggacatggaatgctatttgaactttaagaagtaaatatttttacattaatattgcttaaaaggtagaaaagctgttttctt
tggtaatctcaaattttgcatttcttttttagatgataaaaacaatgggcttatttagcatgacaactagtacacattcttttgcagtcaagtgatctaa
ttgccaaacaatgcaagagttacaaagacggctcatagctcaagctgaagaaagacaaacgtgtatccagcagctttcaaggcaacaatacatttcaaag
caatatgaaatactgcaaaacaatgtattgaaggcacttatactagtaacacagcctaaagttccctttagtagaaacgtacacactgcaatggttggat
tgtatgcagcactcaaattgcagctcactagaaaacttcattaaatcttaagtagaagttcactatgagcttacaatatgaaaatcagtatttttgcatc
aaccctgggcttgtatggctgcacagcagctggaatt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: