XB-CLONE-3594059
Clone Name: IMAGE:7794228
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Gene:
tmem131
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168032 [+]
ggttgagtgacagcgcggaaagaccccaaagcgaagaacggtaaagtaacccagttagtcagggcgtatcgaggaacgatccgaaaaccagtcagagggc
agcgaggaggcagcccgaccagctggcaccaaatctggaagcagccacatagttggtgtcagagtgactgcatgagcgcggggttgactgagactgagac
accctttgcacccagattgaaggagagcagagacagtcggggagtcgcactgcctcccttcctatgctgtattcctgagttcctaccctgctgctccaca
cagcatggcccgtatccggcccttcagcctcctgtgctgctcctgctgctcctcgcacggacacaacaacaacaactcccaggagagcagcagccgcatt
tcttgcaatggcatctacagcgtgctcatcgccctgctcaccctgcacaccgcccgcacccacaccgaggcgttcatccaatccgaaaacgtaattgaag
tgcttcgctttgatgatggaggccttttagaggctgaaacaacaattggcctgcgctcctatcagcagaaaagtatgtcattacacagaggaaattgtag
acctataaggtttgagccacccatgctggacttccatgagcagccagtgggaatgccaaaaatggaaaaagtttacttgcacaatcctagttcagaagaa
actattacattaatatctatatcagcaacaacatcgcattttcatgcgtcgttttttcaaaataggaaaattctcccaggtgggaatacgtcatttgacg
tggtgtttcttgcaagagtagtgggaaacgtagaaaatactttatttatcaacacctcaaatcatggggtttttacttatcaggtttttggaattgggat
tcccaacccttatcgactacgccctttcattggggcaagggtccctgtaaatagcagtttttcacctttaattaatattcacaatccacacagtgaacct
ttacaggttgtagaaatgtattccagtgggggtgacttgcacttggagcttccaacaggtcagcaggggggtaccaggaagctgtgggaaatcccacctt
atgagacaaaaggtgtaatgcgtgcaagcttctcttcgcgagaagcagacaatcacactgcatttattaggattaaaacaaatgcctcagacagcgcaga
gtttattatactgcccgtggaggtagaagtcaccacagctcctgggatatattcctcaacagaaatgttagattttggtacattacgaacacaagatgca
cccaaagttttaaatctccaccttttaaattcgggaatgaaggacgttcctataactagtgttcggtcgacacctcaaaatgatgcagtaacagttcact
tcaagccaataacactgaaagcttcagagagcaaatacaccaaagtagcaagtatcagttttgatgcatcaaaagcaaagaagccatcacagttttctgg
aaaaatcactgtcaaagctaaagagaaaagttattccaaattggaaattccatatcaggcagaagttctggatgggtatttgggatttgaccatgctgcc
acactattccacataagggacagcccatctgatccagtagaaagagcaatatatctaacaaacacctttaattttgctgtcctcatacatgatgtcttgt
tacctgaagatgcaaaacctatgtttaaaatccagaatttcagctcccatgtattaattccaccaggtgaatctcgctatatattttcacttctcttcac
accgtccacatcttcagttcatatagacagcaatatattacttattaccaatgcatcaaagttccatcttccagtgcgtgcttacactggatttctagat
tactttattttacctcctaaactggaggaacgttttatagattttggcatcctaagtgcttcagaatctagcagtcttctaattgccatcatgaatagca
accctatagagttagccatcaagagctggcttgttattggagacagtctttctatagatctgctcactacagagaaaggcaacagaacctccatcattgc
aagccttccagagattgaaaatgccacagtgtcagaccgcacctcagttatattaacttcaggttattatgcggtgttccgtgtctctattattgctaaa
gatttggaaggatcctatgatggaggcatacaaataacaacagattatgagatactaactgttcctgtaaaggcagtggtagctgttggctcattgacgt
gctctccaaagcgcataactcttccaccttccttcccgggaaaagtagtccatcagagtttaaatataatgagttccttctcacagaaggtgaaaataca
acaaatacgctccttatcggaggatgttagatttttttataagagattacggagcaataaagatgaattagagcctggaaagaagtcaaagatagcaaac
atttattttgatgctgctttaaattgtggagaccactgctatattggacttccttttttatttaaaagtgaatctaagggacaacatttagcaatgcaag
aggacttgtgggattctgactgggattattatgagaatttatacaagaagtggtcagaaattcgtgaaaattctgatcatttacttgaagctgcgtttga
ggttgacactgacctccagaaaaatatcctttcagatgtgaaagctgaacttatgtggccttcagttgttagttcatcaagatatattcgctttccattg
acgaataccaacagctcaacagtggaagaaatgttgttagaaaatccttctgatgctccagtttacatccagatccttccactggctctctattctaaca
attcattcttgttagaaagaataatagacagatttaacattagtaagtcagttgatatagacctgaaaacccttgaattccaagtcttcagaaataatgt
aaaccctgtgcagagcagtcacagctttgtggagggctcctcacgccactccgtactgaaccttgttttaaagtctggggagaaacaaaatgtgaaatta
aaattcactccagttcagaatcaaacggtatcctctttacttattgttagaaataatttaactgtaattgatgtgattatggttgaagggcagggagcaa
aggaaaaccttaaggttgcgggaaaacccccaggccctggaagctcattgcgctttaaaatcacagaagcttttctgaaagactgcacagacaaaattaa
acacagagaaccaaacttcacaatgaaaaggacatttaaacttgaaaacacaggacagcttgacgtgactgttaaatccatggaaatcagtggatattcc
tgtgaaggatatggattcaagattttgaattgcaatgaatttgcactaggtcctaatgcctcaagggaggttaccatattgtttacaccagatttcacct
cctcacgagtgatccgtgaacttagaattgtaacagaaggtggttctgagtttgtttttatattaaatgcgtcccttccatatcacatgctagctacatg
cgcagaagcacttccacgacccaactgggaactggcactttatgtcatcatctcagggataatgagtttgctgtttctattagtaattgttacagcttac
ttagaagctcaagggatatgggaaccgtttaaaaggcggctttcctttgaatccgcacacccttccattgtttttggacgaccctttgacctcagaagaa
ttgtgggcatttcatcagaaagcagctttaattccgttggatcagactccaatcacagttctaacagagggctatacacagggagtacctccacgaggac
tggcgcaggtaatcagaagccatgcaacccagtatccaacctgcagaacagtaggagtgctcctgacgcagaactcagtcggccaaagaacagctcaaat
atagcaagcaaattatcttcacaagcagctgttgcacaaccaagtagtaaagccccttccccgcaaacagactcaaatacagttgttcaaaaccaagctt
taaccaggcgcaccaggggcacaagactgagtcagaatcaagtgtcacaggaacagcttccatctacaccatcaccagcagtgcctccaccccaagcaga
acagcaagcagaaatctcacggccggtgaccagtaatacttcccatccagagtactttcgggctagcagcagtaatgcccggcatagctcagatgattct
gatataactaatcttatagaagctatggataaagactttgatcagtctgaaatcacacattcagatgtgtttacagaacagcccccatctccagtgctta
aaaataaagggaaaacaagatctgttatgcacaaatccaaatttcaaaagaagcaagaagagaaagacagaagaataaaaggcaagcagctggaggatga
gcttaaagactcccttgctgatgacgacagctcctcaaccactacagaaacctctaatcctgatactgagccccctgtcaaagaggatggggaaaagtta
aagggaaaacaaaccattcctgttaaatcagaaagagaaattttgaaggtgaagccgaaaggcaagaaagccgcaaacaacaaaaaagaaagcattccgg
aggttaagcaaagctccttggaattaccatataccacacctcttgaaaacaagcaacgcaaaaatttctccaaaattacagtacagaacccattactgaa
tggttcaaaaccaaagactttgcagaaagcaagaggtaccagcagtaagttagtggacaacaggcagtcactggcaaaatatctcccttgtcgcactggt
caggaactgggcaacaccagcagttcagaaggggagaaagattccccaccgccagagtgggactctgtgccaattcataaagcaggcagctcagcagaca
gcctttataaactgtctttacgaacacaccatgcagatgactttttgaaacaacgacaaacctcacctgctcctacctctccgtcccctcccccctcggc
aagctttgtggcaagaggcagctacagcagcattgtcaatagcacaaataatcttgatctaaggagcaaccttactggtaccaaacacaagctaactaag
gcaacctccttacctggacgaaatggcaaccctacctttgctgctgtagctgcaggatatgacaaaaacccaggtggtaatggagtggcaaaaccagccg
ttactaaatgtgatgccccaagcaacatttgtgtctctcataccatttcccttgacagcgatggctctgatagctctggcctatggagccccatcagcag
tcccaacagcccagacttagttccacttaaatcattctctgcttttggacccagcaattccttcaacttaacaggggaggtgtttaataatttggcattt
ccaagatcagcctatggccctgatcctcagaagaaatggagtgagtttgagtcaacatcatcaatctgggatgcacccagcactgaccctgttcccacat
ggccaaccagcacaggatctcccacccatgcatcaccttctatccttgggaacactagcagtctctggtctacaaccacccctttcagcagttccatatg
gtcaagcaaccctaacaacagccttccatacagcactcctgccaacactcttcctggcattgatctgatgggcagtgaaagcgtctgctcttcctccaac
tctgcttcggcagctgtttcacacaaccccgacgccatcggaggccagacttacaatccttggaggatttggagtcccaccattgggaggagaagctctg
atccatggcccaacacccattacccacctgataattagagctctggtgaatttggtctcttttcgtggagcagaaaaaactgcctgtgatttgattggat
gagtgtccgtttgttctttcaaacaggctttttatcttaatggtgttttatttgatttttttttttaaattccaacacctggccttcttagaatccacct
cttaagtataacttctactggttttaattctcccttttgccttctgttctgtttacttgatattgctgttccgttttaagaagggcaaaaaaaatatttg
gcaaagtccttgccttacagaagttacccacacagactgtttatagctgttccgtttcctccccctccatgtttatttatttatgttgccactgagccct
gtaataattggcccagagtgaacaatatcagccccgtcagataagcatcaccaaggaaaaagagatctattttttttatgtgacaagcgaggatctcccc
agtattatgtttctaataaaggcacatagccgcctgtgctacaatgctgagtatttttcatgcaaagtttgtttctgtgccggctaagtgaacaaaatgc
agcaagatgagagtgcagaaagcgggatgttatggctgtgcagctcttccatttgtgttcttggttatctaccgggatgcattttatagttaagttgaat
tgctcaacaaaaaaatcttgtgattaaaaacgaggttagtggggcaattcatgcaactttatgttgtaccttaaataaatgaagcttttttcagtaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaa
5' EST: CX907184 [+]gttgagtgacagcgcggaaagaccccaaagcgaagaacggtaaagtaacccagttagtcagggcgtatcgaggaacgatccgaaaaccagtcagagggca
gcgaggaggcagcccgaccagctggcaccaaatctggaagcagccacatagttggtgtcagagtgactgcatgagcgcggggttgactgagactgagaca
ccctttgcacccagattgaaggagagcagagacagtcggggagtcgcactgcctcccttcctatgctgtattcctgagttcctaccctgctgctccacac
agcatggcccgtatccggcccttcagcctcctgtgctgctcctgctgctcctcgcacggacacaacaacaacaactcccaggagagcagcagccgcattt
cttgcaatggcatctacagcgtgctcatcgccctgctcaccctgcacaccgcccgcacccacaccgaggcgttcatccaatccgaaaacgtaattgaagt
gcttcgctttgatgatggaggccttttagaggctgaaacaacaattggcctgcgctcctatcagcagaaaagtatgtcattacacagaggaaattgtaga
cctataaggtttgagccacccatgctggacttccatgagcagccagtgggaatgccaaaaatggaaaaagtttacttgcacaatcctagttcagaagaaa
ctattacattaatatctatatcagcaacaacatcgcattttcatgcgtcgttttttcaaaataggaaaattctcccaggtgggaatacgtcatttgacgt
3' EST: CX907183 [+]accgaaaaaagcttcatttatttaaggtacaacataaagttgcatgaattgccccactaacctcgtttttaatcacaagatttttttgttgagcaattca
acttaactataaaatgcatcccggtagataaccaagaacacaaatggaagagctgcacagccataacatcccgctttctgcactctcatcttgctgcatt
ttgttcacttagccggcacagaaacaaactttgcatgaaaaatactcagcattgtagcacaggcggctatgtgcctttattagaaacataatactgggga
gatcctcgcttgtcacataaaaaaaatagatctctttttccttggtgatgcttatctgacggggctgatattgttcactctgggccaattattacagggc
tcagtggcaacataaataaataaacatggagggggaggaaacggaacagctataaacagtctgtgtgggtaacttctgtaaggcaaggactttgccaaat
attttttttgcccttcttaaaacggaacagcaatatcaagtaaacagaacagaaggcaaaagggagaattaaaaccagtagaagttatacttaagaggtg
gattctaagaaggccaggtgttggaatttaaaaaaaaaaatcaaataaaacaccattaagataaaaagcctgtttgaaagaacaaacggacactcatcca
atcaaatcacaggcagttttttctgct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: