XB-CLONE-3591896
Clone Name: IMAGE:7687308
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Gene:
mms19
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121492 [+]
CAGGAGTGTGTGAGTCGCTTGCGTCGCTGCAGCGCAATGGCTGAGGCTCACAGTGCTCTATGGGGGTTGGTGGAGGAGTTTGTGGTTGGAGAGCAGGATT
CCAGGTCTGCCGAAGTAGCAGCAGGTGTAAAAGATGGGATCTTCACTGTGCTGCAAGTGGTGGAGGCACTTGGCTCTTGCCTTGCAAACCCTGAACCCAG
ATCGCGAGCTAAAGGCATGCAGCTTCTGTCAAGAGTTTTACTGGAGTGCTACTCCAGGCTTACAGAAAAAGAAGTGGAAGTCCTGGTTTTGTTTTATGAA
AACCGATTGAAGGACCATCATCTCATTACACCGCATGTCCTCAAAGGACTGATGGCATTGAGCATGTGCGATGTGCTCCCTCAAGGTTTGGCCGTGTCTG
TGCTTAAATCAGTTTTTCAAGAAGTCCATGTTCAGTCTTTAATGCAGATTGACCGACACACAGTCTACATGATCATCACTAATTTCATGAAGACACGCGA
GGAAGAGCTGAAGAGTCTTGGAGCTGATTTCACTTATGGATTCATTCAAGTTATGGATGGAGAAAAGGATCCACGAAACCTCCTAGTGGCCTTCCACATT
GTGCAGGATATCATTACTAAGAACTATGCACTTGGTCCTTTTGTGGAAGAGCTGTTTGAAGTGACATCATGCTATTTTCCCATAGATTTCACTCCTCCAC
CCAATGATCCACATGGCATTACCAGGGAACACTTAATAGTGGGCTTGAGAGCTGTTCTGGTGTCTACATCACGTTTTGCAGAGTTCTTTCTGCCACTCTT
AATTGAAAAAATGGACTCTGATGTGCAAAGTGCTAAGCTGGATTCGCTTCAGACACTGATTGCCTGTTGTACAGTTTATGGACAGAAGGACCTGAAAGAA
TTCCTCTCTGGTCTTTGGTCATCCATACGGCGGGAGGTATTTCAGACAGCAAGTGAAAAGATTGAGGCAGAAGGTCTAGCTGCCCTACAAGCACTCTCTG
CCTGCCTTTCTCGCTCAATACTCAGTCCTGATGCTGAGGATTTGCTGGACTCATTTTTAAACAGCATACTGCAAGATTGCAAGCACCATCTGTGTGAGCC
GGATATGAAATTGGTGTGGCCCAGTGCCAAACTTTTGCAAGCTGCTGCTGGTGGGTCTTCCAGAGCCTGTTGGAAAGTCACAGCCAATGTCCTTCCTTTG
CTTTTGGAACAGTACAACCAGCATGCTCAGAGCAGCCATCGCAGGACAATCCTAGAGATGACATTGGGCTTCTTGAAGCTGCAGAGCAGGTGGCTTGATG
AAGAAGATGACAATGGTCTGGGGAATTTAAAGGAAGCCTTGTGTACCATGGTATTTTCAGCAGTGACTGATTCCAGCACTCAACTGCATCAAGTGGCTGT
GAGAACCCTGACTGTCCTAGCGATGCAGCAAGGTTTTTTGTCATCCAGTGATATTGACCTGGTCGTCGATCATTTGACAAGACTCATCTTACAGGAAACA
GACAGTGAAAGCTGTATGGCAGCCATTGAGGCTTCAGGAACCCTGGCTAAAGTTCACCCCTCAGTGTTTATCAGTAGGATGCTGCCACAGCTCTGCGCAA
ACTTGCAAACAGAACCAATGGATATTAATCTGAATGAAAGCAGAGTGGTCCCTGAGCGTTTTATAAGGCAGCGATGTCTAGAGGCATTAGCCGCTGTATC
CACACATCAGAGTATTCTTAAAGAAACTGTTCCTATCCTACTGGACTATATAGGGAGGGTGCACAATGGAGAAGGGGAGACAAATGCAGAAAATGTGGTG
TCTATATGTAGGAGCCTTCATCGGGTGGCTGTACAGTGCCAACTAGATTCCGAGGCTCTTCAGTTTTACCATGAAATTGTGTTACCCTCCCTTCTGTCAT
TAACTGTACAAGCTGCAACACAAGACAGCGGAACTTCCTGCAATGTTTTGCTGAGGGATGATGTTCTCACTGCAATGGTGCCAGTAATTACTGCAGCCTG
CACCCATCTCACACCCGAGTTAGCCTCAAAAAGCGTCTCCCAGGTTGTAGCCTTATTTCTTGATGGGGATGTTTCACTTTTTTCTGAAAACAATTTACCA
AGCAACTTCCAACCATTCCAGGTAAATGGTCCCACGGAGCTCCAGAACCACTTAGTATCATTGTTAATGGCATTTGTTTGCTCCTTGCCAAGAAATGTGG
AAATTCCACATCTGAGACGATTGCTGCAGCATTTGTTATCATTGAGTCTCTCAGGGTGCTCACCATTTGCATATTCCTCTGCCTCAAAATGCTTTGCTGG
GCTAATCAATAAATGTCCCCAAGGGGACCTGCTGGATGATATACTTCTAGTAACAGCTCAACGGATAGATGTGGGGCTGGTGGATGAACCCAGCAGGACT
CGGGCCATTACTCTCCTAGTCTGGGTAACCAAAGCCTTGGTACTGAGATACCACCCTTTGAGTGGGCAGCTAACCAATAAGATGATTGGTTTACTGTCTG
ATAAGCAGCTGGGTCCTTCAGTGGCAAATATGTTCTCCCTGTTAGTATCAGACTCGCCGGATATCATTAATAAAGCCTGCCATGCAGACATCCGCATCAT
GTTCCGGCAAAGGTTCTTCACAGAGAATGTCCCAAAGCTGGTGCAAGGTTTCAACTCTGCCAACAGAGATGACAAGCCAAACTACCTGAAAGCTCTCTCC
CACGTGCTTAATGCACTGCCAAAGCAAGTTCTCATGCCGGAGCTTCCATCGTTATTATCCCTGTTGCTTGAGGCCTTATCCTGTCCTGACAAAGTGGTCC
AGTTGTCAACTCTGACCTGCTTAGAACCTCTGCTGCAAGAGGCGCCGGAGACCCTGAAAGTGCACATAGACGGTCTTATTTCCAAGCTCGTCAGTCTGAC
CTTAAGCCCTGCAATGGCTGTTCGGATTACAGCTTTGAAGTGTATCCTTGCTCTCACCAAGCTCCCCTTGCACATGCTCCTGCCTTATAAGCAACAGGTG
ATCCGTGCCTTAGCCAAACCTCTGGATGACAAGAAAAGACTAGTGAGGAAGGAGGCAGTCGAGACTCGCTGCCAGTGGTTCTTGCTTGGAAGTCCAGGGA
GCTGAGAACAGACTGGATTCTAACTTTGCACGGAGACAATCTAGCCAGGATGCCACTTATTCTTACGACATGATCAACACCATGACTCCATGTGCCATCT
TGTTTAGTCGCCCCTCAAATACTGACAACCCCAGGCCCTAACATAGAAGCGGGACCTGCTCACGTCATTAACTCACTGCTAAGTGGATCACAGACGTCCT
GCAGCTTTATATTGCACATGAACGATGGTAGCACAGATGTACATTAGATCAATGACTTGGCATGTGGATGAAGAGAACTAGTCCATGAAATATATATAAG
GTGTATATTATAACGTATAGTTCTGTGAATGAATAGCAACGCCAGTCTCTTGGCCTGGTTTTAAGTTGGACATAAAAGTTGTTTTTTTTTTTTTTAAAAA
AAAAAAAAAAAAAAAAAAA
5' EST: CX909471 [+]caggagtgtgtgagtcgcttgcgtcgctgcagcgcaatggctgaggctcacagtgctctatgggggttggtggaggagtttgtggttggagagcaggatt
ccaggtctgccgaagtagcagcaggtgtaaaagatgggatcttcactgtgctgcaagtggtggaggcacttggctcttgccttgcaaaccctgaacccag
atcgcgagctaaaggcatgcagcttctgtcaagagttttactggagtgctactccaggcttacagaaaaagaagtggaagtcctggttttgttttatgaa
aaccgattgaaggaccatcatctcattacaccgcatgtcctcaaaggactgatggcattgagcatgtgcgatgtgctccctcaaggtttggccgtgtctg
tgcttaaatcagtttttcaagaagtccatgttcagtctttaatgcagattgaccgacacacagtctacatgatcatcactaatttcatgaagacacgcga
ggaagagctgaagagtcttggagctgatttcacttatggattcattcaagttatggatggagaaaaggatccacgaaacctcctagtggccttccacatt
gtgcaggatatcattactaagaactatgcacttggtccttttgtggaagagctgtttgaagtgacatcatgctattttcccatagatttcactcctccac
ccaatgatccacatggcattaccagggaacacttaatagtgggcttgagagctgttctggtgtctacatcacgttttgcagagttctttctgccactc
3' EST: CX909470 [+]aaaaaaaaaaaaaacaacttttatgtccaacttaaaaccaggccaagagactggcgttgctattcattcacagaactatacgttataatatacaccttat
atatatttcatggactagttctcttcatccacatgccaagtcattgatctaatgtacatctgtgctaccatcgttcatgtgcaatataaagctgcaggac
gtctgtgatccacttagcagtgagttaatgacgtgagcaggtcccgcttctatgttagggcctggggttgtcagtatttgaggggcgactaaacaagatg
gcacatggagtcatggtgttgatcatgtcgtaagaataagtggcatcctggctagattgtctccgtgcaaagttagaatccagtctgttctcagctccct
ggacttccaagcaagaaccactggcagcgagtctcgactgcctccttcctcactagtcttttcttgtcatccagaggtttggctaaggcacggatcacct
gttgcttataaggcaggagcatgtgcaaggggagcttggtgagagcaaggatacacttcaaagctgtaatccgaacagccattgcagggcttaaggtcag
actgacgagcttggaaataagaccgtctatgtgcactttcagggtctccggcgcctcttgcagcagaggttctaagcaggtcagagttgacaactggacc
actttgtcaggacaggataaggcctcaagcaacagggataataacgatggaagctccggcatgagaacttgctttggcagtgcattaag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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