XB-CLONE-3591688
Clone Name: IMAGE:7687767
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Gene:
amfr
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121831 [+]
AGCGTCCAAATAGGGCCAGCAGCAAGCGGTGCGAGCTGCTCCCACCCGCGGCGGGCCCTCCCTTCGGTGTGACAGGCCCTTTGGACTAAGGCAAGATGCC
GCTGTTATTCCTGGAGCGATTCCCCTGGCCCAGCCTGCGCACTTACACCGGCCTGAGCGGCCTGTTGCTGGGCCTCAGCCTGCTAAGCGCCTATCGGAGC
CTGAGCGGGGAGGCGGCAGAACGGAGCATGCTGGAAGGAGAGGAAGCTGGGGCACAGCTGCCACCTGAGCCCGTGGACTTAGTTGAGCTGTTCCGGTACC
TGGTCTCTGACAGTCTTGGGGTGTGGGTTCTAGTGAACACAGTGTGTTGCCTTTTGATGCTGATTGCTAAACTGATCCAGCATGTGGTATTTGGTCCCCT
CCGTGTTGGAGAGAGACAGCACTTGAAGGACAAGTTTTGGAACTTCATCTTTTATAAATTTATCTTCATCTTTGGGGTACTCAATGTCCAGACAGTAGAG
GAAGTGGTCCTTTGGTTTTTGTGGTTTGCTGGATTGCTTTTTCTTCATCTCATGGTGCAGCTCTGTAAGGATCGGTTTGAATACTTATCTTTCTCCCCAA
CCACTCCAATGAGTTGCCATATGCGTGTCTTGGTTCTGCTGAATATTATGCTCTTGGCCTGTTGTGGTCTTGCTGTGCTGTGTGCAATTGCTGGATACAC
ACATGGGATGCACACATTGGCTTTTATGGCTGCTGAGTGCATGTTGGTGACAACAAGAACCACTCATGTTGTGCTACGCTATGTCATTCACCTTTGGGAC
CTAAACCACGAAGGAACATGGGAGGGCAAAGGGACTTATGTATACTACACAGATTTTGTGATGGAGCTCAGCCATCTGTCCATAGACCTAATGCATCATA
TTCACATGTTGCTTTTTGGTAATATCTGGCTGTCCATGGCAAGTCTTGTCATCTTCATGCAGCTCCGCCACCTCTTTCATGAATTTCAGCGCCGAATACG
ACGCCACAAAAACTACTTACGAGTTGTTGGAAACATGGAAGCTCATTTTGCTGTAGCAACTCCTGAGGAACTTGAAGCTAACAGCGATGACTGCGCTATC
TGCTGGGATTCAATGCAGTCTGCTCGGAAGCTTCCCTGCGGTCATCTTTTCCACAACTCATGCCTCCGATCCTGGCTGGAGCAGGACACGTCATGCCCAA
CTTGCAGGATGTCCCTTAACATGGCTGATGGCACAAGAACTCGTGTAGAACAGCAGAGGGAAAATCAAGATCAGAATGCGGTACCACTGGCAGTTACAGA
AGGACGGCCACGTCTCAACCATCATAATCACTTTTTCCATTTTGATGGTTCTCGATTTGCTAGTTGGTTGCCTAGCTTCTCAGTGGAAGTAATGCATACA
ACCAACATCCTTGGCCTTGCACAAGCAAGCAACTCTCAGCTGAATGCTATGGCCCGGCAGGTTCTAGATATGTTCCCACAAGTTCCGTATCATTTGGTTT
TGCAAGATCTGCAGCTTACGCGTTCTGTTGAAATTACCACTGATAATATATTGGAAGGACGTATTCAAGTTCCTTTTCCCACACAGCACCAAGACGATGT
CAGATCTGCAACAGAAACATCTGAAGTCAGTGCACAAGAAGAAAGCAGTACTGCCACACAGACTGAGCGTGTAAATTTGGATTTGAGCCCAGGTCTTGAG
GAATATGAGGATTTCAGCGTAATCGAGGCTCAAGTGAGTGATGTCGAAGAATTTGAGGCCAGAGGCAGCAGATTCTCCAAATCTGCAGAAGAGAGGCAAC
GGATGCTTGTCCAGAGGAAAGAAGATATGGTTCAACTTGCACGGAGACGTTTTTTAAACAGGACTTCAGAGGAAGACAATCCTGACTGTTCATCAGAGAG
GGGTTCACACACAGACAGTTCTTCAGATTCAACGACTGTGCGGAGGCGCATGCTGGCTGCAGCGGCAGAAAGAAGAATTCAGATGCAACAGTCCCCACAG
TAATCCCAGTGTGCATGGACACAACCTAACCTTGAGCCTTGTACAATGGATGCACACATTGACACTTTCTTACTTTGCTCCTTGTTCATGAGCTGTACCA
GCTCACAGAGAGAATTTAATCTTTTATAATCTTATTTTTTTGCAATATGTTTAGAATAAGCAAAGCCATTTCTTGGTTCTAAAGGCAAGTTTGCGCATGT
GATCCTGGATCATTCTAACTCTTCTCTGATTGGCATCCTAATGAGGAGGAACCGTCAGTATTTTAAGCAGAGTAGGTGCCAGAATATTAAAGTAACATAG
GGTTTTTTTTTTTTTACAATGATATTGACTAACTAACATAAATGTTCAGAGAAATGGAACTGGATGAACAACATTAAATGATCTACACCAGGTAGTTGGA
ATCTGTTTATATTACTTTAATAGTTTTCAGATGTCAGATCAATTTTTATCAAATTTCTGTTGTCATAAAACAATTGCATGAATACTGTGTTGCTTTATCT
AGAGTGCTTCAAGAAGGTTTGGTGATAATACTTATTTTTTAGATGTTATGCCAAATGAAAGTGATTTGGAAGCGTTAAACTGTTCTGTAAGATATTTAAT
GGGGATGTTCACTTACACAGCACTGTAGCTTAATAGGTATGGTCTGTACAACATCTGCAGACTGTAAAACAAAGTAAAATCTGGCCACCAAAATATCATG
ATTTACATTACCTAAAAGATCAGTTTCCTGCCAAAGCAGGAGGAGCTTCCCATTAGATCTCTTAAAAAATTTTCCTCTAAAATGTATAAGTGGGGCAAAT
GCAAAAGTGCTTCTGTCATACTGGGCATAATGTGCCAATCTACTTCTGTCAAGTTTAGGTAGAATTGTTAACCCCATATGTGAGGAAAAATAACTGACTG
AAATGCTACCTGTGCAGGTGGTTATTACACTTGCAGCGCATATTTCTTAAAATCTATATGAGAAATCTATATGAGAAATATATATCATGAGAATAACAAA
ATAACATTTTCAAGGTGAACTTCCTTTTCAGTACTTAAATATAATAGTTAATTTCAAAATATTAGCTTTAACTATAAAACCGGACAATAGTTGGTAAGGT
TTTAGTCTGGTGAATGAAGGGTGCAGGTTTGCAGTAAAAAGTGGAAGTGTAATAGAACTGAGATAAGAAAATTTATTGGGGCTTTTCTAATGGGCTGTGT
TATAAAAACCAACCATTAACCGTGGTTCATGGTTCTTGTGTGCAGTGTGTTAGCTGGGAAACTTAGAATTATCCAGAAATCATGCAGGTCTTTTTAGGAT
TCTGGTGTTACAAGGATTTAAGAAGGCCACTCTTACCTCACCGTAAGTTTTGCTTAGTACAGGAAAATATCATGTACCCGGTTTCATGGTATTTCTATTA
GGGAGTTGCAAAACCTTTATAAAAAATAATTAACAATGTTCAGTATTTCCAACATTAAAATAAAGTTGTCTAGTTTATCTCCAATAGAATCCTCAGATAC
TGCAATGTCTCATGTCCCAGTGAGATTAGGACATCAAATTCACACCTGTCCCAACTGCACGCACTGTTTTTATTTAATAACCTAGTATGGAACTTGCTGC
TGGCACCTTCTCTGTACTTATATACAGTCATTTATCACTGTTTGGTAAATAACTGATATGCCGCAATTACTTGATATTTATCCCTTGGTTTATCTAGTTA
TTTACACAATACATCATTACAAATAACTATTAGATACTTATGTGGGTGTTTAACACCTTTGAGTGCTGGCCACGTGGGCCATAACAGGTGCCGTGCTTTG
TAGAACAATTTGCTTTTTCCATTAGGGGAAATAGGAACTGCAAGCAAAGCGATTACAAGCTGGTTCTTCATTGTCAATACCGTAGAAAATATGAATTACA
GTGCTTAATTGTTAGGGACTAACTTGTGTAGGGCACTAGGCTGCTTTTGATGTTGATTCTGTTGGAGATGGCCACTACAAAGAAAGAATCTAAAAAATGA
TAAAGATAGTAGCTTCTCATTCTGGTTTTTCTTTCCTGGCTGCCTGTTACACACACAATGTCTAAAAGAAAGGTACACGTGCATGTGTGGATTCATAGAC
AATGCATTTATTTTCTGCAGTGTTACGCAAACAAGCCAAACTGCTTTTGCTTTGTTACATGAAAACTTGCACCTTGTTTCAAGAGGCGCATCAGTATATT
TCTACCCAATACGTAACTTCGTTTAGAAGGTCTGTAAAGTGTCATTCAGAAAACATTTTTCATGCTACTGCCAGCACGGAATCATTTTGCCTTGTGATTT
GGGCTACTGCTTTTGTTTTTTATTTAAAATAAAATTTGGATCAGTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAA
5' EST: CX909674 [+]agcgtccaaatagggccagcagcaagcggtgcgagctgctcccacccgcggcgggccctcccttcggtgtgacaggccctttggactaaggcaagatgcc
gctgttattcctggagcgattcccctggcccagcctgcgcacttacaccggcctgagcggcctgttgctgggcctcagcctgctaagcgcctatcggagc
ctgagcggggaggcggcagaacggagcatgctggaaggagaggaagctggggcacagctgccacctgagcccgtggacttagttgagctgttccggtacc
tggtctctgacagtcttggggtgtgggttctagtgaacacagtgtgttgccttttgatgctgattgctaaactgatccagcatgtggtatttggtcccct
ccgtgttggagagagacagcacttgaaggacaagttttggaacttcatcttttataaatttatcttcatctttggggtactcaatgtccagacagtagag
gaagtggtcctttggtttttgtggtttgctggattgctttttcttcatctcatggtgcagctctgtaaggatcggtttgaatacttatctttctccccaa
ccactccaatgagttgccatatgcgtgtcttggttctgctgaatattatgctcttggcctgttgtggtcttgctgtgctgtgtgcaattgctggatacac
acatgggatgcacacattggcttttatggctgctgagtgcatgttggtgacaaacagaaacactcatgttgtgctacgctatgtc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: