XB-CLONE-3591512
Clone Name: IMAGE:7687887
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Gene:
khdc4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125755 [+]
ACGACGCGGTGGGAGGGACGCAGAAGCGGGCAGTTGGCTAAGTGGTCGCCATGTCAGCGGGAAGCGGACGAAGAAGCAAATGGGACCAGCCAGGCCCCCC
CAGCACCACACTCCTCCTCCCCGGCGTCCTGCCCGCCATTGTCCCCTTTACTGCTGGCGCCTTCCTCAGCCCACCCGAAATGCCACCTGTAACTGCCACA
TGTGAGTCCGTCAGCGCTCCATCTGGGGCCCTGGATGCCGCAGCAGCCGTGGCAGCTAAAATAAATGCCATGCTTATGGCCAAGGGGAAGATAAAGCCAA
CGCAGAATGCCCCCGAAAAGGTTCAAGTACCCGGCAAAGCGCCCTCTGCAGCCAAAAGCAAAGATGACCTGGTAGTGGCAGAAGTGGAGATCAACGACGT
ACCCCTGACGTGCCGGAATCTTCTTACAAGGGGGCAGACGCAGGATGAGATAAGTCGCATGAGCGGCGCGGCGGTATCGACACGGGGGCGCTACATGACT
GCAGAAGAGAAAGCCAAAATAGGGCCTGGAGATCGGCCTCTCTATCTGCATGTCCAGGGCCAGACACGGGAACTTGTTGATCGAGCCGTGAACAGAATCA
AGGAAATCATCACCAACGGAGTGGTAAAAGCAGCCACCGGCTCCAGCCCGACCTTTAACGGAGCCACAGTAACCGTGTATCACCAGCCGGCCCCCGTGGC
GCCCGTTGCTCCTCCCAAACCTCAGTTCCAGTCAGGGATGCATTATGTACAAGATAAGCTGTTCGTGGGCCTGGAACACGCGGTGGCCACTTTCAATGTG
AAGGAGAAGGTGGAAGGCCCAGGGTGCTCGTATCTCCAGCACATTCAGCTGGAAACGGGGGCCAAGGTCTTCCTACGAGGCAAAGGGTCGGGTTGTATTG
AGCCAGCCTCGGGGAGGGAGGCCTTCGAGCCAATGTATATTTATATCAGCCACCCAAAGCCAGAGGGACTAGCGGCTGCCAAGAAGCTCTGTGAGAATCT
CTTGCAAACAGTCCATTCAGAATACAATAGGTTTGTCAACCAGATCGCCACCACCGCACCTCTGACTGGTTACGCACAGCCGCCTCAGATCGGTGCAGTG
CCCATGCAGCCGCAGTACTACCCTCCTAATGGCTACCAGACTGGCTTTCCCGTGGTCCAACAACCTGCACCCCAGCCGGCTGTACAGGTTCCCTATGTGG
TTTCTACACCCATTGGCAGCCCAGTGCCCCCACTGCCTGGAGTTGTACCTACCATGGCAGCTCCGGTGCCTCCGGTTCCAGCTGTGCCAACCCGTTATCC
AATCCCACAGGTGCAGCCGCCAGGTTCCACTGTTCCAGCTCAGCTGACTGCTCCGTATATGCCTGCACCCCATGGTAAACATGCCGCTGCCGTAGTGACA
CAGGCTCCGCTCCAAGGTCAGAAGCGACGCTTCACGGAAGAGCTTCCAGAGGAGAGGGACTCCGGGCTGCTGGGTTACCAGCATGGACCCATTCATATGA
CTAATTTAGGTACAGGCTTCCCGGCCCAAAGCAAGGTGGATGGAGCGACAATCAAATCGGACACCATGGTCGTCAAAGAGCGAGAGAGGGACAGGCAGTT
GATGCCTCCGCCCGGTATGCCAGTCTCTGCACAGAAGGAGCCGGAGGAAAAGTCATCACCCGGGACCGTTGGGGTTGCAGATGATTATCCAGTAAAAAAG
CTGAAGAGCTCAGGTAAAACCTTCGGCTTGGTCGCTTACGCCGGGGACTCGTCCGACGAGGAGGAAGATCATGGTGTGCTAAAGAGCTCTGGTTCTTTAT
CCCAAGGCTGGAATGCAGGATACCAGTACCCAGCTAGCCAGCAGCAGCAGCGCGCCAAGCCACAGATGCCATTCTGGATGGCGCCGTAGGACTCTATCAG
AACCTCATCCTCCCGGAACCGCCTGTAGCAATAATCCAGCATGTACATTACACAAGGGCCCTTTTTACTTTGCTGATTTTTTTTTTTTTTAAACAACGGG
ACTTGGTAAAGTAACAGGAGTTTGTATGGAAAGTGGGATCCCTCGCCCCCTTTTAATTACCCACAATCCAACAGCCCCAGTGAGCCAGAGATGGTTCCCA
AGCAGATCCTCCACTCTGGGGAATGGTTTGGGCTCCTCCCACCACGACGTAGGCAGGCCATCAATATTTTTTATTTATAGACTTTTATTTATACGTAGGA
GTTCAGGAGTCTGTCTTCATTGGAGGAGTGCCACCAAAGCACCGCCCCCTTGTGAGATTAGGGGAGGAGACTAGCAATGGTCAACTGCAACTCCCAGTGT
CATAGTGGTTACTTGGGAGTTGCAGTTTAACATTTGGTCAGGGACAAACAGTACCACAAACAGCAGATTTTCCTTTTAATCTAAGCCAGAGAGCAAATAC
TGTCCTCAGTTTGTTGCCACTATGGCCTCTCTATCAGGGCAAGGCTACAAACCCTCAGCTAGATACGTATAGGCTGCCAGCATTCTATTTCTGATAAAAG
AAAGTTTTAGGCTACCATGTTTTATTAAGGTGCCAGCTCCATGGGGGCACTCCCAGTGTATATACCCAGCAGTATTCCCACCCGGTGGTGCAGTGTTTAA
AGGGGAACTGACATGAAGGCAAAAATCTTCATCTGCTCCAAATAAAACGCTTCCACATAATGTATTTATTGTTACTCTTCGCTCTCCCCTTGCAGCAGTC
GGTCGGCTTTGTGCCTGTGAAAGAAAACTTACCTGAGCGCAGGTAGTTGGTGCAATGTAGCGGGGATAGGGAAGAGTCGTTTGTTGTATTAAGATTTTCT
GTCTTTTATGATACTTCCCCTTTAAATGAAATGGCAGCAGTTTAATGTGGCTTCCAGTGAGGGGAAAATCCCCAGTATTCCATTTAATTTCCTAGAGCAG
TAGGGCCCCCCTAGTGCCCCCTATAGAGACAATACCAGCTTTAAGGCTTCTGTATTTAGGAAACTAGCTCCGCCTTGTGTTCCTCTGCCTTCAGCTCTTG
CACTAGCTCTGCCTGCATTCAGCTCTCTATGCATCAAGCAACCCCCCCCCCGGCACAAACCATTTGACCTGTTTATCCCCCCCCCTCCAACAAGTTGAAC
TATACCAGATTCTGGGGTCCCACAGCATCTGTGTCGCTTCTGTCGGCCAACACTGCTCCCTACTTCCCAATGAGTATTCTTTTTGCTGTAAATATTTCAG
TTTTACTTTTATTGTAAATAAATGAATAATTATACAGCTAAAAAAAAAAAAAAAAAA
5' EST: CX909860 [+]acgacgcggtgggagggacgcagaagcgggcagttggctaagtggtcgccatgtcagcgggaagcggacgaagaagcaaatgggaccagccaggcccccc
cagcaccacactcctcctccccggcgtcctgcccgccattgtcccctttactgctggcgccttcctcagcccacccgaaatgccacctgtaactgccaca
tgtgagtccgtcagcgctccatctggggccctggatgccgcagcagccgtggcagctaaaataaatgccatgcttatggccaaggggaagataaagccaa
cgcagaatgcccccgaaaaggttcaagtacccggcaaagcgccctctgcagccaaaagcaaagatgacctggtagtggcagaagtggagatcaacgacgt
acccctgacgtgccggaatcttcttacaagggggcagacgcaggatgagataagtcgcatgagcggcgcggcggtatcgacacgggggcgctacatgact
gcagaagagaaagccaaaatagggcctggagatcggcctctctatctgcatgtccagggccagacacgggaacttgttgatcgagccgtgaacagaatca
aggaaatcatcaccaacggagtggtaaaagcagccaccggctccagcccgacctttaacggagccacagtaaccgtgtatcaccagccggcccccgtggc
gcccgttgctcctccccaacctcagttccagtcagggatgcattatgtacaagataagctgttcgtgggcctggaacacgcg
3' EST: CX909859 [+]agcgggataattattcctttatttacaaaaaaagtaaaactgaaatatttaccgcaaaaagaatactccttgggaagtagggagcagtgttggccgacaa
aagccacacaaatgctgggggaccccaaaatctggtatagttcaacttgttggagggggggggataaacaggtcaaatggtttgtgccggggggggggtt
gcttgatgcctaaagagctgaatgccggcaaaactagtgcaaaaactgaaggcaaaggaacccaagggggagctagtttcctaaatacaaaagccttaaa
gctgggattgtccctatagggggcactaggggggccccactgctctaggaaattaaatggaaaactggggattttcccccccctggaagccccattaaac
tgctgccctttcatttaaaggggaagtttcctaaaagaccgaaaatcttaatacaacaaaggactcttccctatccccgctaaattggaccaactacctg
ggctcagggaagttttctttcacaggcacaaaacccaccgactgctgcaaggggagagcgaaaagtaacaataaataccttatggggaagcgttttattt
ggagcaaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: