XB-CLONE-3591001
Clone Name: IMAGE:7687965
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Gene:
atg4d
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122931 [+]
GACCCAAAGCCCAGGGGCCGCCCGATACTAACTTTCTCCCTTCCATTACCCAGAAGCATTGGCGCCGCAGTCCCCGCCCCCTCCGGCTTCAGCGTACGGC
GCTTATATACGCGACTGTGACGTCAGAGTATCGCGATATGAGACTTGGGACGTCAGCTTTAGCTGCCCGGAAGGGATCGGGGGGATTGTTGTTACCGGCT
CTGCGGGACGTTTAAATGATGATGCGGGACCGTATAGCTATGAGTGCGGGCACGGTGTGGCACGTCCCTCAGCGCTCCTATTAGTGACCCGTGAGACAAC
TGCAACAACGTCGGACGGAAACCCCCACTTTCCAATCAACGTGGCTCCGGTTTCAGGTCTGTGGGAGGCCTCAGCAGTTAAATGAATTCTGTGTCCCCGC
TGGCCACGCAGTACGGAAGCCCCAAAGGTTCACAGCAGACAGAGAACCGCAGGACGCCAAGTGGGCACCATGAGCAGAGAAGGGTGGGCTACCAAGATGT
CACCGCAGAGGGCGAAACTGATGAAGTGGATAAACTCAAAAGCAAACTGCTGACAGCTTGGAACAATGTTAAGTATGGGTGGTCAGTGAAGATGAAAACC
ACTTTCAGCCGCTCTGCACCCGTGTATTTACTGGGAGAGCGCTACTACTTCCGGCTGGATGATGAAATTGATCGGTTTCAGAAGGATTTTGTGTCCAGGG
TGTGGCTAACATATAGACGTGACTTTCCTGCCCTCGAGGGCACAGCGCTCACTACAGACTGCGGATGGGGCTGCATGATCAGGAGTGGCCAAATGCTACT
GGCACAAGGTCTGCTCCTGCACCTTCTTTCCCGAGAATGGACTTGGTCGGAAGCCTTATACACCCACTTCGTGGAAATGGAACCCATTCGTTCTTCCTCT
CCATCCAGTATGCCCCTCTCCTTGGCCACAGACCATTCGGGCAGGCACTCCCAGCCCCAGACTCACTGTTCGAGAGCCCCTTATGGAGGTGAAGTCCATC
AGAACATAGTGAGCTGGTTCTCAGACCATGCAAGCGCCCCCTTTGGCCTGCACCGCATGGTGGCATTAGGAAGCATCTTTGGCAAGAGGGCAGGGGACTG
GTACGGCCCCAGTATTGTGGCACACATTATAAAGAAAGCAATAGAGAGCTCTTCTGAAGTCCCAGATCTGTCCGTGTACGTGTCTCAGGATTGCACAGTG
TATAAGGCAGATATTGAGCAGCTCTTTGCCGGAGAGGTACCGCACACAGATACCAGCAGGGGGGCTGGCAAGGCTGTCATTATCCTGGTGCCTGCGCGTC
TTGGCGGAGAAACCTTCAACCCCGTGTATAAACACTGTCTCAAGGAGTTCCTGCGAATGCCGTCCTGTTTGGGCATTATTGGCGGAAAACCAAAACACTC
GCTCTATTTCATTGGATACCAGGACAATTACCTGTTATACCTCGACCCACATTACTGCCAGCCGTACATTGATACCAGCAGGGACAACTTTCCGTTAGAG
TCTTTCCACTGTAACGCTCCTCGCAAACTCTCCATCACCAGAATGGATCCCAGTTGCACCTTTGCTTTCTATGCCAAAAACCGCGATGACTTCGGTAAAC
TCTGTGAGCATCTGTCGAAGGTGCTGCACTCCCCCCAAGCCGAGGAGAAATATCCCATTTTCAGTATCTCAGAAGGTCAAGCTCAGGAATACGCAAGTGC
TGAGGGGCAGCAGAGCTCCTCTCACCCCCCCGTGTGTCGCAAGAAAGGCCCGATGGTGAAGAGACCCAGCTCGGATGAGTTTGAATTCCTGTAAAGCGCA
AGTGTTCTGACTGGGAGTTCTAAAGGCCCAGTAAGCCTGTTACAGTGCGGTACCGCTGCTAGGGACAATGTTACTCACTGCTGTTAGGTCACTTACCTGC
TGCCTCTGCCTTTATCCCTACATGGGTTTGGGTACAGGGCATCTTTACTCTTCCACTGGCCAACCCTTAGCCTTGTTCTGTGTGTACCCCCACCCCCCTC
CCCCCCAATCTTTTGGAACAAAATCTTAATTATGAAGAAACCTGTCCTGGAGATTGGGGGTTCAAATTCAACCCAATAAATGGAGCTTGTATGGAAACAT
CAACCACATGCCCTTTTGTGAGTTTATGGTACAGCCCATAATCTCCATGGAGCATCCAATGGTAGGCCAGCTTCTTAGCTCTGTATATTACAAGCTCCCA
GGCCAAGCAGTAAAGGGCAAGTCTGTGCAGCCCTGGATATGTCTAGAAAAGATTGGCCAGTTGGCCCAGATCATCAAGCAGTATGTGCTCACTCTCCAGA
ACTGGACTAATGCGGGTGTAGTCGTTGGGCTAACAATCTATAACCATGGTGGCGCTCAATGCTACACTGCAAGCAGGCCATTAGGGTAGTGCTAGATGTA
GGGGTCTGTCTTAGTGCTTAGTACTACAGCTATTGGGCGGGGTGGAGAACCCAGCCCTGCTTATAGAGTCAGAAATATAAACTACTACTGGTAGTAGATA
TAATCAGGGGATAAACGGATACTGTACACTGAAACCATTACTCTGTTTGATACTCTCAGGCATAAGCAAACTGAGGCCCTTAGTGTATGTAGTGCAGTAA
TATCTGGACAACTAGGGCATGCTGTATGTAAATGTGTAGTTGAACAACATTGGCCGGGCGCCTGGTTAGACCCCCCGACCATGACATAGAGCAGCTATGG
TTCCGATAGATACAGGGGCTCCCTTACTCCTGTGTGCCAGTGGGAAGACTAAAGGTGGGTGGCAGACCCCCCCCATGCTGGAATTGCTGTTGTTTGTAAG
GCAATACACTGGCTATATGTGTGTGTGTTTGTGTGTACATGTCGATTTTAAACTGGAAAAGAAAAGTGAAATCTGTATATTCTGGCAAGTTTTTATTTAT
ATACGGGAAATAAACCTTTTTAAAATGGGAAAAAAAAAAAAAAAAA
5' EST: CX910432 [+]cccaaagcccaggggccgcccgatactaactttctcccttccattacccagaagcattggcgccgcagtccccgccccctccggcttcagcgtacggcgc
ttatatacgcgactgtgacgtcagagtatcgcgatatgagacttgggacgtcagctttagctgcccggaagggatcggggggattgttgttaccggctct
gcgggacgtttaaatgatgatgcgggaccgtatagctatgagtgcgggcacggtgtggcacgtccctcagcgctcctattagtgacccgtgagacaactg
caacaacgtcggacggaaacccccactttccaatcaacgtggctccggtttcaggtctgtgggaggcctcagcagttaaatgaattctgtgtccccgctg
gccacgcagtacggaagccccaaaggttcacagcagacagagaaccgcaggacgccaagtgggcaccatgagcagagaagggtgggctaccaagatgtca
ccgcagagggcgaaactgatgaagtggataaactcaaaagcaaactgctgacagcttggaacaatgttaagtatgggtggtcagtgaagatgaaaaccac
tttcagccgctctgcacccgtgtatttactgggagagcgctactacttccggctggatgatgaaattgatcggtttcagaaggattttgtgtccagggtg
tggctaacatatagacgtgactttcctgccctcgagggcacagcgctcactacagactgcggatggngctgcatgatcaggagtggccaaatgctactgg
cacaaggtctgctcctgca
3' EST: CX910431 [+]cccattttaaaaaggtttatttcccgtatataaataaaaacttgccagaatatacagatttcacttttcttttccagtttaaaatcgacatgtacacaca
aacacacacacatatagccagtgtattgccttacaaacaacagcaattccagcatggggggggtctgccacccacctttagtcttcccactggcacacag
gagtaagggagcccctgtatctatcggaaccatagctgctctatgtcatggtcggggggtctaaccaggcgcccggccaatgttgttcaactacacattt
acatacagcatgccctagttgtccagatattactgcactacatacactaagggcctcagtttgcttatgcctgagagtatcaaacagagtaatggtttca
gtgtacagtatccgtttatcccctgattatatctactaccagtagtagtttatatttctgactctataagcagggctgggttctccaccccgcccaatag
ctgtagtactaagcactaagacagacccctacatctagcactaccctaatggcctgcttgcagtgtagcattgagcgccaccatggttatagattgttag
cccaacgactacacccgcattagtccagttctggagagtgagcacatactgcttgatgatctgggccaactggccaatcttttctagacatatccagggc
tgcacagacttgccctttactgcttggc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: