XB-CLONE-3590960
Clone Name: IMAGE:7688019
|
Gene:
cenpo
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX910480 [+]
agtagatatgcggctgcgagatggggtagcaccggcagttcctaggcaaagcccaaatcacatggagtatgtggatatgaacaaactgagctgcgggcga
aagtcccgggtggaaggaggccaactggccggcgacgaatggacccggcacggcagctttgtcaataagcccacccgaggctggctgcaccccgacgaca
aggtcatgggccccggggttccctatctggtcaggtacatgggctgtgtggaagttctgcagtcaatgagagccttggacttcaacacccggactcaagt
cacaagggaagccataagcctggtgtgcgatgctgttccaggagcaaagggggcaatgcgacggcgaaagacttgtgggcggtccttaaactccatccta
gggaagagcaacctgaagttcgccggcatgccaatcactctgaccgtctccaccagcagcctcaacctcatggcctccgactgcaaacagatcatcgcca
accaccacatgcagtcaatatcatttgcttctgggggagatcccgatacagctgagtatgtcgcttatgtggccaaagatccagtcaatcagagagcctg
ccatatcctggagtgcccagaaggccttgctcaggacgtgatcagcacaataggacaggcctttgaactacgcttcanacaatacctcanaaatcctccc
ccccccacctctctgtaccttgtgc
3' EST: CX910479 [+]gtatagaaatactactttaataacaataagaagcagactatgggagttcctttggtataatcgaggcatatgatgacaataaaacaataagaaagtagga
cataaacatgggggggtgagagagagaggggggtacagttgtgcggcacgatatgcagagtgtgtgcagcggctttggtgtcggggtacaaagtgacatg
caatacaaagatcagatttatgtacatggggggggggggggagtgaatcattatttcccccaaacagattagcggggtacaccccctggccattcccttc
ctctcaagttgcaccccactctttcctccacagcagggatgctagactccccccagtagcatttagtagaactgccaattatttccactgggtttaaatg
attccatttcccatgagtccctttccccctggaggcgggcaatcttgctttatcaaagccccgcccactccattatgctcaatgcatactcagccctcaa
gtaatgaggtccctaagggaatagtatgtgccctaagggctcacgtaagagggccaatcagctctgtgtaaataagcccctcccttcccacagatgccac
tgcctctcaggcttacagagaaggaggatttccttatagagtatgtc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: