XB-CLONE-3589671
Clone Name: IMAGE:7688969
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Gene:
pde5a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121494 [+]
GCAGACAAAGCCACAACATGGTTACCAAGCCAACATCTAAGTCTTAAACAGTGCACGATTATTACAATGTTATACATGAGGCAGATTTGTACGGTTTTAT
TGTGGGTCCTGCAGACTGGTTGACTAATTTAGAAGTCTTCCTACAAGCTGCAGAACGTGTTAGAATCAGCTGTACATAGCTTGTTTTTATGGGTCATTCA
TCACAGCTGCAGTGGATTTTACTAGCAGCCTAAAGATTTCTTTTCTGCATCTGCCTGTTTTCAACCTTCAAATACGGACTGTTAATTGGCGATGAAGGGA
ACAAGGGTCGAATTCCTAAATGACAAGACCACCAAGTGAAATAATTAATAACATCGAAATGATAATTTTAGTGGAACATTATTAACACAGGACTGGGCCA
TTTCTAAGAGAGGTAACTCTTGTAAGAGAAATGGTCAACGCATGGTTTGCTGAACGAGTTCATACAATACCAGCTAGTAAAGAGCAAGGAAAAAATAATT
CAGAGTCTAATCCTCAGGTGTGCTATTCAGAAAATACCACTCAGGGAACTCCAGTGAGGAAGATCTCAGCCTCAGAGTTCGATAGGCCCCTAAGGCCAAT
TGTTGTCAAAGATTCTGTCGGAACACTGAAATTTCTTTCTGATTCTGAAAAGAAGGAACTGATGCCTCTTCAACCACCCAGTCTGTCCAGAGCAGGAGAT
CAGTGCTCAAGGCTTTTGGAACTGGTGAAAGATATTTCAAGTCACCTTGACGTCACAACACTTTGTCATAAAATTTTTTTGCACATCCATGAGCTAATAG
CAGCAGATCGTTATTCGCTCTTTTTGGTTTGTGAGGACAGTTCCAATAAGAAGTTTCTGGTCAGTAGACTGTTTGACGTGGCAGAAGGTACAACTGTGGA
ACAGGCTTCAAACAATTGCATCCGTTTGGAATGGAATAAAGGGATTGTTGGTCACGTGGCAGAGTTTGGTCAGCCCTTGAATATAAAGGATGCTTACCAG
GACTCCAGATTCAATGCGGAAGTCGACCAAATTACTGGGTACAAAACACAAAGTATTCTTTGTATGCCAATTAAGAACCACAGGGAAGAGGTGGTTGGTG
TAGCACAAGCAATCAATAAGAAGTCAGGAAGCAATGGGACATTTACTGACCAAGATGAAAAGGACTTTGCTGCTTATCTGGCATTCTGCGGAATTGTTCT
ACACAATGCACAACTTTACGAAACATCACTGCTTGAAAACAGGAGGAACCAGGTGCTGCTTGACCTTGCAACCTTGATATTTGAAGAACAGCAATCACTG
GAAGTCATCTTAAAGAAGATCGCAGCTACCATTCTGTCATTTATGCAGGCTCAGAGATGCACAATATTCATTGTGGATGAAGATAGTCCTGATACCTTTT
CAAGTGTGTTTCATATGGAGTCTGATGAATTAGAAGAGACTGCAGATGCTGTAAAAAGGGAATATGATGTGAGCAAAATGAATTACATGTATGCACAGTA
TGTAGCAAATACAATGGAAGCAATTAACATTCCAGACATATGTAAGGACACAAGATTTCCATGGACAAACGGAAATGAAGAGAAGAATTTCAAGCATATT
CAGAGTTTGCTTTGCTCCCCAATCAAGAATGCAAAGAAGAATAAAGTAATAGGAGTCTGTCAACTTGTAAATAAGGTGGATGAAAACTCTGGCAAAATTA
AAGCATTCAACAAAAACGATGAACAGTTTCTAGAAGCCTTTGCCATATTCTGTGGCTTGGGAATTCAGAACACCCAGATGTTTGAAGTTGTGGAGCGTGC
GATGGCCAAACAGATGGTGACATTAGAGATTCTTTCATATCACGCATCTGCTGCTGAGGAAGAAACAATGGAGCTGCAGGTAACAGCGGGAGCCATTGTG
CCATCTGCACAATCGCTTAAACTGACAGAATTCTACTTCAGCGATTTTGAGCTTTCTGACATGGAAACCACATTAGCCACAAGCCGAATGTTCACAGACC
TCAACCTTGTGCAGACCTTCCAAATGAAATATGAGACCCTTTGCCGGTGGATCCTGAGCGTGAAGAAGAACTATAGAAAAAATGTTGCATATCACAACTG
GAGACATGCCTTTAATACAGCCCAGTGTATGTTCGCAGCCCTGAGAACTGGCAAAATTCAGGGTAAGCTAAATGACCTAGAAATCCTTGCACTGATGATA
GCCACCTTGAGCCATGACTTGGACCACAGAGGAGTTAACAATTCTTTTATACAGAGAAGTGAACATCCTCTAGCACAGCTATACTGTCATTCTATCATGG
AGAACCACCATTTTGACCAGTGCCTAATGATACTAAACAGTCAGGGTAACCAGATTCTAAGTGGGCTTTCTGTTCAAGACTACAAGACTGTTCTGAAGAT
GATAAAACAAGCCATCCTTGCTACTGATTTGGCACTATACATTAAGCGAAGAAGTGATTTCTTTGAATTAGTGAATAAGAAGAAATTCAAATGGGATGAT
CCTTCACAAAAAGAACTGTTCTTAGCAATGCTGATGACGGCTTGTGATCTGTCTGCAATAACAAAGCCATGGCCAGTTCAACAACGGATAGCTGAACTTG
TAGCCTCAGAGTTTTATGACCAAGGGGATAAAGAGAGGAGGGAACTTAATATTGAACCAATAGACCTGATGAACAGAGAAAAGAAAGACAAAATACCCAG
CATGCAGGTTGGATTTATTGACGCAGTCTGCTTAGAACTTTATGAGGCCTTAACGCAGATCTCTGAATCGTGTTACCCTTTACTTGATGGCTGTCAGAGA
AATAGGCAGATGTGGCAGATCTTAGCAGAGCAGCAGGAAAAGAACTTGGTGAATGGGGCGAAGAGCCAGCCTGAAGTGAAGTGCAACATGGATTAGAAAA
TCACAAGAAACCTGTCATTTTGTGGTACAAAATAGCATTACACTGTCTGAAGAATTTTAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX911841 [+]cagacaaagccacaacatggttaccaagccaacatctaagtcttaaacagtgcacgattattacaatgttatacatgaggcagatttgtacggttttatt
gtgggtcctgcagactggttgactaatttagaagtcttcctacaagctgcagaacgtgttagaatcagctgtacatagcttgtttttatgggtcattcat
cacagctgcagtggattttactagcagcctaaagatttcttttctgcatctgcctgttttcaaccttcaaatacggactgttaattggcgatgaagggaa
caagggtcgaattcctaaatgacaagaccaccaagtgaaataattaataacatcgaaatgataattttagtggaacattattaacacaggactgggccat
ttctaagagaggtaactcttgtaagagaaatggtcaacgcatggtttgctgaacgagttcatacaataccagctagtaaagagcaaggaaaaaataattc
agagtctaatcctcaggtgtgctattcagaaaataccactcagggaactccagtgaggaagatctcagcctcagagttcgataggcccctaaggccaatt
gttgtcaaagattctgtcggaacactgaaatttctttctgattctgaaaagaaggaactgatgcctcttcaaccacccagtctgtccagagcaggagatc
agtgctcaaggcttttggaactggtgaaagatatttcaagtcaccttgacgtcacaacactttgtcataaaatttttttgcacatccatgagctaatagc
agcagatcgttattcgctctttttggtttgtgaaga
3' EST: CX911840 [+]aaaattcttcagacagtgtaatgctattttgtaccacaaaatgacaggtttcttgtgattttctaatccatgttgcacttcacttcaggctggctcttcg
ccccattcaccaagttcttttcctgctgctctgctaagatctgccacatctgcctatttctctgacagccatcaagtaaagggtaacacgattcagagat
ctgcgttaaggcctcataaagttctaagcagactgcgtcaataaatccaacctgcatgctgggtattttgtctttcttttctctgttcatcaggtctatt
ggttcaatattaagttccctcctctctttatccccttggtcataaaactctgaggctacaagttcagctatccgttgttgaactggccatggctttgtta
ttgcagacagatcacaagccgtcatcagcattgctaagaacagttctttttgtgaaggatcatcccatttgaatttcttcttattcactaattcaaagaa
atcacttcttcgcttaatgtatagtgccaaatcagtagcaaggatggcttgttttatcatcttcagaacagtcttgtagtcttgaacagaaagcccactt
agaatctggttaccctgactgtttagtatcattaggcactggtcaaaatggtggttctccatgatagaatgacagtatagctgtgct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: