XB-CLONE-3589293
Sequence:
Full length sequence: BC161088 [+]
agctcctgtcagaacccgggtgggtgtcgggcagcgcgggcattgcctgtgagagagccgggatggagccaggccatgattggatgtgaccccccccctc
acccggactaaagatggcgggtggtcctaaagcattgcccctattcaacatggcggcgcactgccactgctggctcctggctctggtgttcttggtgctc
tgcgggtccatggctagttcgtccgcggccggagaggacagtgaggagaccgtcattatagggctgcggctggaggatacggacgaggtgtcggccatgc
acggcggagcgctgcgggttagcgagcggacccgggtccggttgcgggtttacggtcagaacatcaacaacgagacgtggtcccgcatcgccttcactga
gcacgaacggagccggagcaggagcggggccccgggggaagaggaaggcttccacccctgcgggatccgcacctcggacatcatcatcctgccttccatt
gcactgagccgccgcagctccggcatcgtggagatcgatatcaaacccctgcggaagactgagaagagcaagtcctactacctgtgcacctccatctcca
cgccacccagtggggggcacccacagccctgggcagagaccacctggatctatcatgatggggaggacaccaaggtgattgtggtggaagagaagaagtt
cttgttgcctttctggcttcaggtcatcttcattgccatgttgctgtgcctgtccggcatgttcagcggcctcaacctggggctcatggcgctggatccc
atggagctgcgcattgtccagaactgtggcaccgagcgggaaaagaactacgccaagcgcattgaaccggtccgtagacagggcaactacctgctctgct
cgcttctgctgggcaatgtcttggtcaacaccacattgactatcctgttggatgacatcgctggatctggactggtggccgtggtggtgtctaccattgg
gattgtcatcttcggagagattgtgccacaggcgatctgttccaggcatggactagctgtcggggccaacactatcttcttgaccaaattcttcatgatg
atgacctttcccgcttcctaccctgtaagcaagctgctggactgtgtgctgggtcaggaaataggcactgtctataacagggagaaactgttagagatgt
tgagggtcactgacccctacaatgacctagtcaaagaggaactcaatatcatccagggtgccctggaactgaggaccaaaactgtggaggatgtgatgac
ccctctccgtgactgctttatgatggctggggatgcagttctggacttcaacaccatgtccgagatcatggaaagcggctacacccggatacctgtctat
gaaggggagaggtccaacattgtggacttgctttttgtcaaggacctggcatttgtggatcctgatgactgcacacctctaaagactatcaccaggttct
acaaccaccccttgcactttgttttcaatgacaccaagctggatgccatgctcgaggaattcaaaaaaggtaaatcccaccttgctattgtgcaaagggt
taacaacgagggagaaggagatccattttatgaagtacttggcattgttacccttgaagatgtgattgaagaaatcatcaagtcagagatcttggacgaa
acagacttgtacacggataataggacaaaaaaagaaggtaatgcaccgggagaggaagcaggacttctccgcttttaaacaaaccgacaacgaaatgaag
gttaaaatatcaccacagcttctcctggcaatgcaccgtttcctagcaacagaggtggatgcatttagcccctctcagatgtcagagaaaatcctgctaa
ggctgctgaaacatcccaacgttatccaagagctaaagttcgatgaacggaacaagaagtctgctgaatactacctctaccagcggaataagcccgtgga
ctactttgttctcgttttacaggggaaggtagaagtggaagcgggcaaggaaggcatgaagtttgaagcaggtgcattttcatactatggcgtaatggct
ctcactgcatcaccagttcccttgtccctgtctcgtacctttgttgtcagcagaacagaatcgttagcaggttctccagcagagaataaatctccacccc
gtccatgtggattgaaccattcggactctcttaacagaagtgatagaattgacgctgtgacgcctacactgggaaatagtaacaaccagctcaatgcttc
cttcttacaagtctatatccctgactactctgtaagagccataacagacatgcagtttgttaagatcacaaggcagcagtaccaaaatgcattaatggct
tcacgcatggacaaaacgcctcaatcctcagacagtgagaacactaaaatagatatgactcttaccgagctacacgacggcttagcagatgaaacagcaa
atctgttaaacgaacagaactgtgtcacacacaacaagtccaaccacagtatgcacaatgaaggtccgatttgagggggttcagcccagctcattgacat
tcaggactgtcctcacttcattctcaagcttccttcccggattcctgtctgtgcaactgcaaacaccttgagtacaagctcatgccgagtgccacccagc
attctgagaccaaagattctgagcccagcggtggcaggaaccgaagtacttaaaacaggagcaggagagagacatcaggaataaagcaaatagtaataaa
taaaactagggattggtgagtgagaccaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
5' EST: CX912237 [+]ctgtcagaacccgggtgggtgtcgggcagcgcgggcattgcctgtgagagagccgggatggagccaggccatgattggatgtgaccccccccctcacccg
gactaaagatggcgggtggtcctaaagcattgcccctattcaacatggcggcgcactgccactgctggctcctggctctggtgttcttggtgctctgcgg
gtccatggctagttcgtccgcggccggagaggacagtgaggagaccgtcattatagggctgcggctggaggatacggacgaggtgtcggccatgcacggc
ggagcgctgcgggttagcgagcggacccgggtccggttgcgggtttacggtcagaacatcaacaacgagacgtggtcccgcatcgccttcactgagcacg
aacggagccggagcaggagcggggccccgggggaagaggaaggcttccacccctgcgggatccgcacctcggacatcatcatcctgccttccattgcact
gagccgccgcagctccggcatcgtggagatcgatatcaaacccctgcggaagactgagaagagcaagtcctactacctgtgcacctccatctccacgcca
cccagtggggggcacccacagccctgggcagagaccacctggatctatcatgatggggaggacaccaaggtgattgtggtggaagagaagaagttcttgt
tgcctttctggcttcaggtcatcttcattgccatgttgctgtgcctgtccggcatgttcagcggcctcaacctggggctcatggcgctggatcccatgga
gctgcgcattgtc
3' EST: CX912236 [+]gggctcactcaccaatccctagttttatttattactatttgctttattcctgatgtctctctcctgctcctgttttaagtacttcggttcctgccaccgc
tgggctcagaatctttggtctcagaatgctgggtggcactcggcatgagcttgtactcaaggtgtttgcagttgcacagacaggaatccgggaaggaagc
ttgagaatgaagtgaggacagtcctgaatgtcaatgagctgggctgaaccccctcaaatcggaccttcattgtgcatactgtggttggacttgttgtgtg
tgacacagttctgttcgtttaacagatttgctgtttcatctgctaagccgtcgtgtagctcggtaagagtcatatctattttagtgttctcactgtctga
ggattgaggcgttttgtccatgcgtgaagccattaatgcattttggtactgctgccttgtgatcttaacaaactgcatgtctgttatggctcttacagag
tagtcagggatatagacttgtaagaaggaagcattgagctggttgttactatttcccagtgtaggcgtcacagcgtcaattctatcacttctgttaagag
agtccgaatggttcaatccacatggacggggtggagatttattctctgctggagaacctgctaacgattctgttctgctgacaacaaaggtacgagacag
ggacaagggaactggtgatgcagtgagagccatta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: