XB-CLONE-3587827
Clone Name: IMAGE:7689516
|
Gene:
scube2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC121936 [+]
AATTTTGATGGGCGAGGAGTGAAAGTTGTTGGAGGGGTGGGTAGTTTGAGGGGGTCTCTAGGGAAAGGGTAGGAGGAGCTAGACTAAAGAAGAGGGTCCA
TGTGGGAGTGTTATAACGGGATCTGAATTGAGGATTGTGGAAGGGCGGAGTTGCAGTGCCTCAGTAGGAGCAGTACAGACTTTCCGATAGAATGTGCTCC
TTGGGGGCGGACCTTTGGATTTTCCTCTTGCTTCTTCCGGCTTGCGCACCTGCGCACCCCGCTGCGCGACCTCCCGCAGGCGTGGATGAATGCGCAGAAG
GCATTGATAACTGCCATGCAGACGCTATATGCCAGGATACACCCAGCTCCTTCAAATGTATTTGTAAAACCGGCTTTAAAGGGGAAGGAACGGATTGTGA
AGACATTGATGAATGCGATAATGAATTTAATGGAGACTGCGTCCATGAGTGCGCCAACATCTCGGGCAATTACCGCTGCACTTGCTATGATGGATTCAAG
TTGGCACACGATGGGCACAACTGCCTAGACGTGGACGAGTGCTCACACAACAATGGCGGATGCCAACATACCTGCGTCAACACGATGGGAAGCTATGAGT
GTCGCTGCAAAGAGGGCTTCTTCCTGAGCGACAACCAGCACACGTGCATTCACCGCTCAGAAGAGGGGTTAAGTTGCATGAACAAAGACCACGGCTGTGC
CCACATCTGTAGAGAGAGTCCCAGGGGCGGAGCGTCCTGTGATTGCCGACCCGGCTTTGAACTGGCAAAGAACCAGCGCCACTGTATCTTGACATGTAAC
CATGGGAACGGAGGATGCCAGCATACGTGCAATGATACAGATAGTGGTCCAGTCTGCAGTTGCCATCCAAAGTACACTATGCACTCTAATGGGAAGTCCT
GCATTGAGAAGGAGGAACCGGAGAGTAATTCCACCGCTGCGTCGGACATGGACAAGCGAGTGAAGCGGCGGCTGGTAATGGAAACCTGTGCAGTTAATAA
TGGTGGGTGCGACCGCACATGTAGGGACACATCGACTGGCGTCCGCTGCAGTTGCCCTGTTGGCTTCACCCTGCAGTTTGATGGGAAGACTTGTAAAGAT
ATTGACGAATGCCAGAACAGTAACGGTGGATGTGACCATTTCTGCAGAAACACCGTTGGGAGCTTCGACTGTAGCTGCAAAATGGGCTTCAAATTGTTAA
CAGATGAGAAGTCGTGTCAAGATGTCGATGAATGTTCTTTTGAGCGGACATGTGACCACACCTGCATCAATTACCCAGGATCCTTTGCCTGTACCTGTGA
CAAAGGTTATACATTATATGGGATCACCCACTGTGGAGACATTAATGAATGCAGCATCAACAATGGCGGATGCCAGCACGCCTGCGTCAACACAGTTGGC
AGTTACTTGTGCCAGTGCCACCCTCGGTATAAACTTCACTGGAACAACAGAGACTGTGTCGAGGTAGAGAGCGCCCCTCCCGCTTTTGTATCGCCAAAAG
CCATTCTGCGTTGTGTTAAGAATGGTGGGAATGAAAGGTGTTTCCTAAGCTGCCAGTCTGATGTTCATTCTGTTCCTGGGCTGCACGATGCTTTCAGTCT
CACCTGTGGATCTCCTGTGCCTCTCAGGTCCCAAAATGGGAGAAATAACGAATCGCTTAATGCAGATGTGGCGACAGTCAGAACAAGCACGACGTTCAAA
GTCACCGAGGGAACGTGCCGAGTGAGGAGGTCGGATCTGAACCAGGATTTCTCCCAGCAGAAAGGATCAGAGAAACACAATGTGCTGTACGAGAGATTCT
GCTTTGTGAATATAACGTGCAGGAGCAGGGCCGGCGCCGGACAGAAGGGAGCGGCCAAGGAAACCTTTCTCACTGCCGAATTTGAGGCTGAATTAAACTA
CAAGGAGGTGACAGACTCCTGCAGCTTCACGTGCGCCCGGAGGAAAGCGGAGAAACGGATGCGCAAGACGTTCCGGACGTTACGCAAAGCCATCAGCAAG
CAGCAGTTCCACGCCCGCCTCTCCGGGAAGGAGCTCGACGTCGGCAAAAAGTCCTCCAGGCAGTCGGATTCACAGGAGATGTGTGGCACAGGGCAAGTAC
GAGTGGACGGCAAATGTGTCAGCTGCCAGGCGGGGACTTACTATGACGGTGACAAGGAACAGTGTGTCCTGTGCCCTAATGGAACATATCAGACCGAGGA
GGGGCAAATAACCTGTGAAACTTGCCCCGGCCCTGAGACCAGAGAGAACCAGAGGACAGCGGGAGCTCGTTATATAACGGAGTGTGTAGGTGTTTGCCCA
CCGGGCGAGTTCTCCGCTGATGGTTTCAGACCATGTGACTCGTGCCCGCTTGGCACCTACCAGCCTGACGGGGGGCGCACCTCCTGCTTTCCATGTGGGG
GCAACCTGATCACCAAGCACCCCGGATCCACTTCATTCCAAGAATGTGAAACGAGAGTTCAGTGCTCCCCGGGACATTCCTACAACACCACGACGCATAG
ATGTGTCCGATGCCCCGTGGGCACCTACCAGCCAGAGTTTGGGCAGAATTATTGCGTATCCTGCCCCGGAAACACCAGCACGGACTTCGACGGCTCCACC
AACGTGACCCAGTGTAAAAACAGGCAGTGTGGGGGGGAACTGGGAGACTTCACCGGGTTCATCGAATCCCCCAATTACCCGGGAAACTACCCGGCAAACA
CGGAATGCACGTGGAACATTAATCCGCCGCCCAAGAGGCGCATCCTGATCGTGGTGCCGGAGATATTTCTGCCCATAGAAGACGACTGCGGAGACTATTT
GGTGATGAGGAAGAGCGCTTCATCCAATTCCGTGACCACGTATGAGACGTGTCAGACCTACGAGCGGCCAATCGCTTTCACCTCCAGATCCAAAAAGCTT
TGGATCCAGTTCAGGTCGAACGAAGGGAACAGCGCCAAGGGCTTCCAGGTCCCGTATGTCACGTATGACGAAGACTATCAGGAGCTGATCGAGGACATTG
TCAGGGACGGGCGGCTGTACGCGTCGGAGAATCACCAAGAGATTCTCAAGGATAAGAAGCTCATTAAGGCCCTGTTCGACGTATTGGCGCACCCGCAGAA
CTACTTTAAATACACGGCGCAGGAATCGCGGGAGATGTTCCCCAAGTCCTTCATCCGACTCCTGCGCGCCAAGGTGTCCCGATTCCTACGCCCTTACAAG
TAACAGCTCCGCCCCCCCCCCACCGCCGGGACTTTATCGTAAGAACAACGCGTTTCGCTTATTCGCTGGAGCAGTCCGGCCCACACCGCTGTGTGTATTG
TAAATATCCGGATAAACGGACTTTCGCTGAACGCGCAGATTAGTACGGAATATAGAGACGCTTCTGGGCCACGGACCCTGCCCAGTATATAATCCAACCA
ATCAAATTGCTTCTACGAGTGCAACTGGGATACCAAGATCTCCCAGGATACCCCATGGGCACCTTCCCACCAAGCCAACCCTCCCCATTAATAACTATCA
GAACCTTCCTCACTGGGTCCATTAGAACCCGATTCCCTCCAGTCTGAGAGAAGGGGGCGTTTCCCTTTGGGTTTCCGAATGCGACGGCGCAACGGATTGG
ACCTTTTGGCTTTGGATTTCAGAACCTAATGATTCCCACTGGGCCGGATGGATTTAACTGCAAGAACACTTGGGGCGTAGGAATATTTCCCCTTGAATTT
GAATCCTTGGTATCAACTCCCTTGTCTATAGGGCGATAAAGCAGCTTTGGTTGAGTTGGGGGGTCATTATAAGGGGACTCCTTTGGCACTGGTTTCTAGG
GTAAGATTACCTGGCAACCAAAGAGTAGATTAAATACCAGAACACAGAACTATTAAAGTGAATATTGCATTCATTATGTGGCCCCCCCTGTGCAAATGGA
CAAGTCTATGGAGTGGATCCTCTCCCACCAATGAGGAAACTGCCCCGGGGCAATTACAGATTTAGCTCCGGCGCCGAGGAGCTGACACTTTATCCGGGGG
CCACACGGACCAATGACTTTGTGATTGTACATAATGTAAAAACGAAAGCTTTTCTAGACTTCATACAGATCGTATCTGTCGGATTTAGAGTTAGGATTTG
GGGGGGGGGAGAGTATTTTTGTAGAGTATAAAAGCGCTTATTCCCCTCCCTGGGATTTACTGCGAGGAGCGAGGGAGGCGGCGCTTACGCACGGATATCG
CTTTATCCGCTTCCGACCAACACAAACGTTCCCCGTAGGATTCCCTGTTCCTCTATATTCACCCTCCGTATGGAACCCAATGTACCCCCGGCTGGTTTCT
ACTTCCCTTCAGGTACCCCCAACCTCATTCTTTATGTAATTGGAACTCTGTATATATCTATAAAGAATAAAATCTCAGTAAATTAAAAAAAAAAAAAAAA
A
5' EST: CX913745 [+]aattttgatgggcgaggagtgaaagttgttggaggggtgggtagtttgagggggtctctagggaaagggtaggaggagctagactaaagaagagggtcca
tgtgggagtgttataacgggatctgaattgaggattgtggaagggcggagttgcagtgcctcagtaggagcagtacagactttccgatagaatgtgctcc
ttgggggcggacctttggattttcctcttgcttcttccggcttgcgcacctgcgcaccccgctgcgcgacctcccgcaggcgtggatgaatgcgcagaag
gcattgataactgccatgcagacgctatatgccaggatacacccagctccttcaaatgtatttgtaaaaccggctttaaaggggaaggaacggattgtga
agacattgatgaatgcgataatgaatttaatggagactgcgtccatgagtgcgccaacatctcgggcaattaccgctgcacttgctatgatggattcaag
ttggcacacgatgggcacaactgcctagacgtggacgagtgctcacacaacaatggcggatgccaacatacctgcgtcaacacgatgggaagctatgagt
gtcgctgcaaagagggcttcttcctgagcgacaaccagcacacgtgcattcaccgctcagaagaggggttaagttgcatgaacaaagaccacggctgtgc
ccacatctgtagagagagtcccaggngcggagcgtcctgtgattgccgacccggctttgaactggcaaagaaccagcgccactgtatcttgacatgtaac
catggggacggaggatgccagcatacgtgcaatgatacag
3' EST: CX913744 [+]aatttactgagattttattctttatagatatatacagagttccaattacataaagaatgaggttgggggtacctgaagggaagtagaaaccagccggggg
tacattgggttccatacggagggtgaatatagaggaacagggaatcctacggggaacgtttgtgttggtcggaagcggataaagcgatatccgtgcgtaa
gcgccgcctccctcgctcctcgcagtaaatcccagggaggggaataagcgcttttatactctacaaaaatactctccccccccccaaatcctaactctaa
atccgacagatacgatctgtatgaagtctagaaaagctttcgtttttacattatgtacaatcacaaagtcattggtccgtgtggcccccggataaagtgt
cagctcctcggcgccggagctaaatctgtaattgccccggggcagtttcctcattggtgggagaggatccactccatagacttgtccatttgcacagggg
gggccacataatgaatgcaatattcactttaatagttctgtgttctggtatttaatctactctttggttgccaggtaatcttaccctagaaaccagtgcc
aaaggagtccccttataatgaccccccaactcaaccaaagctgctttatcgccctatagacaagggagttgataccaaggattcaaattcaaggggaaat
attcctacgccccaagtgttcttgcagttaaatccatccggcccagtgggaatcattaggttct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: