XB-CLONE-3587789
Clone Name: IMAGE:7689616
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Gene:
grb10
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166203 [+]
ggagcaacatgtgccgctgcttgcccagtcaggcaccaggcacccaagggccaccttataaagctgccaccctagccatgggccctcaggtgcttttatg
gataatgtcgatcaaacgacagcttgctccccgagaggtctggtggaaacgggattcacatctaagagaatatcactaaaccctgatgatgatgttgatc
tagaggccctggtaaatgatatgaactcctcactggacaacctttactccacatgcaccagtgtctctgaatctgtgccacttttaaaaaatggtcagct
taacagaagccaactaccaaactcaggaaccaactctcttccgtccatgcctcataaacagaagcttcagcgttcccaaccaatgcatatacatgcagtc
cggcgtctccaagaggaacagctgcgcacatcatctttgccggcaattccaaatccatttccagaactttgcactccagccagttcaccagttcttgtac
cagggtctttacctcaaaatcagccagtaggcaagaaaagtgtggttaaagtctttagtgaagatgggacaagcaaagtggtggaaattctagcagacat
gacagccagggacttctgccagctactggtttaccaaagtcattgtgtggatgataacagctggacgctagtggagcaccaccctcacctaggattagaa
agatgcctagaggatcatgaaatggtggtccatgtacaatcaatgatgtcaagtgaaagcaagtttctgttcagaaagaattatgcaaaatatgaatttt
ttaagaatcctgtgaatttctttccagaacagatggtggcttggtgtcaacaatcaaatggcagcattccccagtctcaattactgcagaattttttaaa
tgccaacagttgccctgaaattcaaggctttttgtatgtaaaggaggtggggaagaaatcttggaaaaaattatttgtgtgtctgcgacgatctggcctt
tactgctccacaaaaggaatgtcaaaggaaccaaggcatttgcaaggaatctcagacctagaagatagcaatatttattcattgataagctgcaagaaac
tttataatgcgccaacagattttggattttgtgtcaagcccaacaaagtgagaaatgaggttaaagagctgcgttttttatgtgcagaagatgaacaaag
caggacttgttggatgacagcattcaggcttctcaagtatgggatgttgctttatcaaaactacagaattccacagcagaaaaaaagcttgcacacaaac
ttttctaccccagtgaggagtgtatcagaaaattccttagttgcaatggatttctctgggcagactggaagagttattgaaaatccagtagaagcacaaa
gtgctgctatggaggaaggccatgcttggaggaaacgcagctcacgactgaatgttcttggtagccaaagtccacttcatccttccacactaagcacagt
aattcataggacacaacattggtttcatggcagaatttctagagaggaatcgcaaactatcattaagcaacaggggcttgtggatgggcttttcctttta
agagacagccagagcaacccaaaggcatttgtgcttacactgtgtcatcaccagaaaataaagcatttccagattttaccatgcgaagatgatggtcagc
tatttttcagccttgatgatgggaataccaaatttatggatctaatacaacttgttgagttttatcagctaaacaaaggtgtcctgccctgcaagctcaa
acaccactgcattagagtggccttatgacctcaggcatagactatagggatggtcttggcttgggtgggaagaaggcatgttttaacatgcaccttgtga
ctctgatgtaagcagctggtgccaactcatcattattctctggtttaaaataggtctacaaggagttgtcatgttccattgtccccagtggcaaagctgg
gtgtgttgactgaaaaaaattaagcaaacacctgcaatcatttttaggctgggttgttcaccagttaaaagtgttgatcaatactgcaagttgggttggc
tagaaatgggaattagtttagcaagtgggtaggtgtgaataaggactattgccctggagtaatcttgacaataaagactggtctgtttactttttttatt
tttaaattgaaatacattttgcactcctggattacggcctttaaaaaagagctggtgcggcattttttaattcatgcagcagatagcttgctgtaatgtg
tgaattgcaacagcctaaaaggattatggggaaaaatcaagatgtttctaagaaggctttgtgctcctgtcacctgcatgtatctttctgaaagctcttc
tggagattggataaagccctagatttcctcttactctttacttaccaataagcgaagtggctgttaaatgtctgtcctaataagcttgagtctgtaagtg
ataactatgcataaatgtttgaactacagttttactctaccttaaatacacctattgtattcatcaggaaatgacatggttttcagacaaaatatggtta
aatagtcacaggctgtatttttgtgaaacatttcaattgtctacctagagtaacaaattttcttctctgtatccatcatctgtatatttttttttcttta
acaaacacttccttggtctaaaggaagataaaataaatacagtactcgtctggaaataggaatttaacataccatgaccgttttctgtggtcttgagcta
agctgcttttgtgtttagttctgtccatagaagggctataaaatagttaaaagtcggggttttgtacctagggatcactcctggtacaagcagaagcttt
agtgattcgtatcagctgtaatttccaacaacaatttagaaacactgtattctggattaaggaacacattctgtcataccgtttttcatgtaaagtctgc
atataaaatgaagaaacttgaaaaaaaaaaaaaaaaa
5' EST: CX913774 [+]gagcaacatgtgccgctgcttgcccagtcaggcaccaggcacccaagggccaccttataaagctgccaccctagccatgggccctcaggtgcttttatgg
ataatgtcgatcaaacgacagcttgctccccgagaggtctggtggaaacgggattcacatctaagagaatatcactaaaccctgatgatgatgttgatct
agaggccctggtaaatgatatgaactcctcactggacaacctttactccacatgcaccagtgtctctgaatctgtgccacttttaaaaaatggtcagctt
aacagaagccaactaccaaactcaggaaccaactctcttccgtccatgcctcataaacagaagcttcagcgttcccaaccaatgcatatacatgcagtcc
ggcgtctccaagaggaacagctgcgcacatcatctttgccggcaattccaaatccatttccagaactttgcactccagccagttcaccagttcttgtacc
agggtctttacctcaaaatcagccagtaggcaagaaaagtgtggttaaagtctttagtgaagatgggacaagcaaagtggtggaaattctagcagacatg
acagccagggacttctgccagctactggtttaccaaagtcattgtgtggatgataacagctggacgctagtggagcaccaccctcacctaggattagaaa
gatgcctagaggatcatgaaatggtggtccatgtacaatcaatgatgtcaagtgaaagcaagtttctgttcagaaagaattatgcaaaatatgaa
3' EST: CX913773 [+]caagtttcttcattttatatgcagactttacatgaaaaacggtatgacagaatgtgttccttaatccagaatacagtgtttctaaattgttgttggaaat
tacagctgatacgaatcactaaagcttctgcttgtaccaggagtgatccctaggtacaaaaccccgacttttaactattttatagcccttctatggacag
aactaaacacaaaagcagcttagctcaagaccacagaaaacggtcatggtatgttaaattcctatttccagacgagtactgtatttattttatcttcctt
tagaccaaggaagtgtttgttaaagaaaaaaaaatatacagatgatggatacagagaagaaaatttgttactctaggtagacaattgaaatgtttcacaa
aaatacagcctgtgactatttaaccatattttgtctgaaaaccatgtcatttcctgatgaatacaataggtgtatttaaggtagagtaaaactgtagttc
aaacatttatgcatagttatcacttacagactcaagcttattaggacagacatttaacagccacttcgcttattggtaagtaaagagtaagaggaaatct
agggctttatccaatctccagaagagctttcagaaagatacatgcaggtgacaggagcacaaagccttcttagaaacatcttgatttttccccataatcc
ttttaggctgttgcaattcacacattacagcaagctatctgctgcatgaattaaaaaatgccgcaccagctcttttttaaaggccgtaatccaggagtgc
aaaatgtatttcaatttaaaaataaaaaaagtaaacagac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: