XB-CLONE-3587456
Clone Name: IMAGE:7689995
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Gene:
syngap1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127326 [+]
gacagaggctgaaggcagaatcagcggtgcaccatgggagttgtatcccggggtgatatttcatatgctcgaggacctcccatgcatcgtatgccctatg
tcacctctccatacgaccgtccagcctggaacccaagatactgtgtcctctctggaaatcagcttctcatgcttgatgaggaggaggttcacccgctgtt
gatacgtgaaaggagaactgatccaaccagaacaaagttcctaaggcgcacagtcagtgtccccgtggaggggaagcagcctgacgaacacgattatcac
cttgcacgttcccggaggaaaagcgtcccagggggaaaacagcccagtatggacgcccctcccaccgccccctttcgaccgtcgcaaggattcctgagcc
gtcgcttgaagagctctataaagaggacaaagagtcagcccaaattagataggaccagcagtttccggcaaatattgccaaggtttcgaagtgctgatca
tgatagggcacggctcatgcaggcctttaaagagtcacactctcacgagtccttgcttagtcctagcagtgctgcagaggctttggatttgaacttggat
gaggattctgtggtgaagcctgtacacagcagcatcttgggacaggagttttgctttgaggtgaccacatcatcaggtaccaaatgttttgcctgccgtt
ctgctgctgaaagagacaaatggattgagaatcttcaaagagctgtaaagcccaataaggataacagcaggcgggtagataatgtcttaaagctgtggat
tattgaagcccgtgatcttccccccaagaagcgctattattgtgagctctgtttggatgacattttgtatgcccgtaccaccagcaagcctcggaatgac
actgtcttttggggggaacactttgaatttaataaccttccagctgttcggaacttgcgcttgcacctctataaggacacagacaagaaaaggcgcaaag
aaaagagcaactatgtgggcatggtgaacgtgcctattgccagcattagtgggcgccactttgtggagcagtggtacccggtttctcagcccacatccta
caaggggaagagcagctgtccctccattcgcctaaaatcccgataccaaaccatgagcatcctccccatggagctgtacaaggagtttgcagagtatgtc
accaacaactaccggatgctgtgcgctgtactggagcctgtgcttagcgtgaagagcaaagaggaggttgcctgtgctttagtgcatatactgcaaagca
caggcaaggccaaggacttcatcttccacatggctgtcactctctttctccccttctcattttctattaacccctttacccccttttccttccactctga
tgaccttctatttctctgtttgtgacactccatctccttctctatcaatctctcattttctctatcactatttctctctgcctcctttcctctctccctc
tctctccccaggacttcctttcagacatggctatgaccgaggttgatcgcttccttgacagagagcaccttattctccgtgagaacacacttgcaactaa
agccatagaggagtatctgaaactgataggacagagatacctcaaggaagccattggtgagtttattcgggcactgtatgagtctgaggaggactgtgag
gttgatcccatgcgttgtccaccctctgcattgcccgatcaccaggccaatctcaggatgtgctgtgaactcgccctttgcaaagtctttaactctcatt
gtgtgtttcctcgggagttgaaggaagtctttgcatcttggaggatgcgctgtgcagagcgcggccgggaagatatagcagatcgactcatcagtgcctc
tctcttcctccgattcctgtgtccggccattatgtcccccagtctcttcagcctaatgcaggaataccctgatgagcaaacatctaggaccctcacgctc
atcgcaaaagtcatccagaacctggccaacttcaactacaagttcagcagtaaagaagaatatatgtcctttatgaatgagttcctagagacggagttgg
cgaatatgcagaatttcctgtatgagatctcaaacctggattcgctcaccaacagcattaactttgagggctacattgaccttgggcgggagctgagtac
acttcatgccctgctttgggaagtgatcccacaacttagtaaggatgcacttataaagttggggcctcttccacgcctccttaatgacatcagcctagct
ctcaggaacccccacatccaacgacaaccaagccaccaagaacgactgccaccccccaaacctgtcattctgaggggaccttcagctgagctgcaaagct
acttggtgcgagacctcaacagctctattgacatgactcgcctaccctcacctactaaggataaaccatcaaaagatatgttttttgtttcacgtcgccc
gctaactcgctcctcgccagcctattgcaccagcagctcagacatcacagaaccagaccagaagatgctgagcgtgaacaagagtgtgtccatgatggac
ttgcaggacagccgaatgaatagcgtaaccaacttgcaggccgtaggtgatctgctgaactccagccaggcctccatatcagggggcctacgtccggggg
gccggctctcccagggcagtggatccagcattgcagcgggtctgaggctcagtcagatgggggtcaccactgatgggctctcagctcaacagctacgcat
tcccttgtccttccagaacccgctcttccacatggctcccgatgggcctggtgggcccccatctcatcctgctcctcatcgaccagaccccggagctgat
gcctttgcccctttccatgggtatagcaagagtgaggacttgagctccggaaagcatatgcttcacagtcacagctacagcgacgaattcagccggcagg
gtaaaaggcagttatccctccaggacaatctccagcacatgctttcaccccctcaaataaccattggccctggagtgccacgaaggcaagactcacagcc
tccccctttgcagaggggaaaatcccaacaactgacggtgcaggcagcgcataaacctcggccaagcagtggcaacctcctacagagcccagaaccaatg
tatggggcagcaagaattaggcagcagtcagttgaaaaggatgcaggtggcttgaaaccatcaataaccaagcagcactcccatgcacaggccactctga
accctgtcactccagcctcagaacgcacagttgcctgggtatccaacatgccccacctttcagcagatattgagagcgctcacatagaacgggaggagta
taagttgaaggaatactctaaatcaatggatgagagccgtcttgagagggtgaaagagtatgaggaagagatttactccttaaaagagaggctgttgatg
tcaaaccggaagctggaggaatatgagaggcgtcttcttacacaggaagaacaaactaataagatgatgcagcaatatcagtcacgcctggaccagagtg
aaaagcgtttgcgacagcagcaggttgagaaggacacgcagataaagagcatcttaaacaggttgatgctggtggaggaggagatcaggaaggatcactc
acaagaaccaatcgatatgaagaggagacttatcgaggctcaggtggaaattacaatgtcattgtgcgtttgtgtgtgacatcttcatcacaccctgtcc
ctgtctttcatctcaagagggagagaagaaaaaaaaaaaaaaagggcggccctcgcgatctagaactagttctagatcgcga
5' EST: CX914107 [+]cagaggctgaaggcagaatcagcggtgcaccatgggagttgtatcccggggtgatatttcatatgctcgaggacctcccatgcatcgtatgccctatgtc
acctctccatacgaccgtccagcctggaacccaagatactgtgtcctctctggaaatcagcttctcatgcttgatgaggaggaggttcacccgctgttga
tacgtgaaaggagaactgatccaaccagaacaaagttcctaaggcgcacagtcagtgtccccgtggaggggaagcagcctgacgaacacgattatcacct
tgcacgttcccggaggaaaagcgtcccagggggaaaacagcccagtatggacgcccctcccaccgccccctttcgaccgtcgcaaggattcctgagccgt
cgcttgaagagctctataaagaggacaaagagtcagcccaaattagataggaccagcagtttccggcaaatattgccaaggtttcgaagtgctgatcatg
atagggcacggctcatgcaggcctttaaagagtcacactctcacgagtccttgcttagtcctagcagtgctgcagaggctttggatttgaacttggatga
ggattctgtggtgaagcctgtacacagcagcatcttgggacaggagttttgctttgaggtgaccacatcatcaggtaccaaatgttttgcctgccgttct
gctgctgaaagagacaaatggattgagaatcttcaaagagctgtaaagcccaataaggataacagcaggcgggtagataatgtcttanagctgtggatta
ttgaagcccgtgatcttccc
3' EST: CX914106 [+]cttctctccctcttgagatgaaagacagggacagggtgtgatgaagatgtcacacacaaacgcacaatgacattgtaatttccacctgagcctcgataag
tctcctcttcatatcgattggttcttgtgagtgatccttcctgatctcctcctccaccagcatcaacctgtttaagatgctctttatctgcgtgtccttc
tcaacctgctgctgtcgcaaacgcttttcactctggtccaggcgtgactgatattgctgcatcatcttattagtttgttcttcctgtgtaagaagacgcc
tctcatattcctccagcttccggtttgacatcaacagcctctcttttaaggagtaaatctcttcctcatactctttcaccctctcaagacggctctcatc
cattgatttagagtattccttcaacttatactcctcccgttctatgtgagcgctctcaatatctgctgaaaggtggggcatgttggatacccaggcaact
gtgcgttctgaggctggagtgacagggttcagagtggcctgtgcatgggagtgctgcttggttattgatggtttcaagccacctgcatccttttcaactg
actgctgcctaattcttgctgccccatacattggttctgggctctgtaggaggttgccactgcttggccgaggtttatgcgctgcctgcaccgtcagttg
ttgggattttcccctctgcaaagggggaggctgtgagtcttgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: