XB-CLONE-3585891
Clone Name: IMAGE:7690934
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Gene:
ercc3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167617 [+]
cgtttccccaataacgtatggatcttcccctgaatggcgacggtgccggtgtcgatttaaataacagttatttacccggcagcattcagagccgttcctt
cccggcaatttgcctctggctcccgggtaatttgccggcacaaagagcggagaagcgtcgggcgccggcagcgggaatctgtttcggcttttgagaaaag
gaggaaaagttcagacttgtaaacgttaaatccaagatctgaaacagggacccccagagagagagcgtgacgcctcctgcccaacgccgctaacgggggc
cgcctggctgccattttatcagtccagatggcagaaatgatactcggtgccaatggtatacagcactgccagccctcgttagcaaacgtgcgcaccggaa
gtggggaagaagcagcagaagaagcagccggaaggtgttgctgccagggaacctgcgatgggcaagagggataaggcggacagaggagacaaaaagaagt
tcaagcggcgtcactatgaggaagaggatgaagaggaagaggaggtgcccgggggcgaataccaggaagccgttccttctgccgcgggaaagcaagtgga
cgaagccggcacaaagcaggatgagtacggagccaaagactatcgcctgcagatgcctctgaaaatggatcattcctctcgccccctgtgggtggcaccc
gacgggcacatcttcctagaagccttctcgccagtgtacaagtacgctcaggatttcctggtggccatatcggaacccgtgtgcagaccctcccacaccc
acgaatacaaactcacggcttattcgctgtacgcggccgttagtgtcgggctgcaaacgagcgacattgtcgagtatctgcagaaactgagcaaaaccgg
ggtccctgaagggattgtacagttcattaagctctgcactgtcagctacgggaaagtgaagctggtcctgaaacacaacaggtactttgtggagagcacc
cacccagaagtcatccaagagctgcttcaagattctgtcatccgaggctgtcggctgcgagacgcagagggaaatgaaactgacctcatcactgagacct
ttctgagcaaatctgcaatatccaaggcaagtgaaacagcagccagtggaccttctacatcccaggtcccagagggacaagacaaaacaaacgtgccggc
tgatttatttgaattttatgaacagatggataaagaggaggaggaggaagaagagacccaaacggtttcttttgaggtcaaacaggacaatatagaagaa
cttcagaagcagtgcatacacttggaataccctctgctggcagagtacgactttaggaacgacacaatgaatccagacattaatattgatttaaagccta
cagcggtgctgcggccctaccaggagaagagcctgcgcaaaatgttcggcaacgggcgggcaagatctggcgtcattgtgcttccttgcggggccggcaa
gtccttggtcggcgtcaccgctgcctgtaccgttcgcaaaaggtgcctggttctaggtaactctgccgtttctgtggagcagtggaaagcccagttcaaa
atgtggtcaaccatcgacgacagtcagatatgccgctttacctccgacgccaaggacaagcccattggctgttccatcgccatcagcacgtactctatgc
tggggcacaccaccaaacggtcgtgggaagcagagcgcgtcatggagtggctaaaaagtcaggagtgggggttaatgatcctcgatgaagtgcacaccat
accggctaaaatgttccgtagagtcctgaccatagtccaggcgcactgcaagctgggactcactgccacactggtacgagaagatgacaaaattgtggat
ttgaatttcctgattggtccaaaactgtacgaggccaactggatggagcttcagaacaatggctatattgccaaggtgcagtgtgccgaggtctggtgcc
ccatgtctcccgagttctacagagaatacgttgccatcaagacaaagaagcgaattctgttgtacactatgaacccaaataaatttcgggcctgccagtt
ccttattaagttccatgaaaggaggaatgacaagatcattgtgtttgcagacaatgtgtttgctctcaaagagtatgccattcggctgagcaaaccttac
atttacgggcccacatcccagggggagaggatgcacattctgcagaacttcaagcataacccaaagatcaacactatctttatatctaaggtaggggaca
cgtctttcgatctgccggaagcgaatgtgctgattcagatctcatcccacggtgggtcgaggaggcaagaagctcagcgtcttggaagagtcttaagagc
aaaaaaggggatggtggcggaggaatacaacgccttcttctattcgctggtgtcccaggacacgcaggaaatggcttattccacaaaacgacagaggttc
ctcgtggaccagggttacagctttaaggtgattacgaagcttgcggggatggaggaagagcatcttgcattctccaacaaggaggatcaacagcaacttc
tgcagaaagtcctggcagcctctgaccttgatgctgaggaggaagtggtggtcggagaatttggatccaggtccaccatgcaggtgtccagacgcgtggg
gaccatgagctccatgtccggagccgacgacgcggtctacatggaatatcacacgtcacgaaacaggggggcgtcgaacaaacacgtgcacccactgttc
aagcgcttccgaaagtgagaagaagcagcttcggcttttccatagcgcaatatggagtcagggcggagccaaggagcgcggacttgtatctatatgtata
aagtacatgcacagagccggcaccagaggattctattggccagtccggtatttcgcccaaactgccacattttgctttatttgttgttgcaatcagttga
tggactgatacggtgcctttatagaggaggagttaacttgtgttatggcggctgccatgtttgtgctcactcggcacttaaaggggaactgtcacccaca
tgtaaaaagctgtataatcttctttacaaattaaacatgaaacccaaattatttttttcattaaatcatccagacctgttataaatgtgtttaaaaatct
gggctgtcagtcatatattgcctgccccgcctctatgccttcggcatagaggcggggcaggcggtgtgggattggcagctcagattttcaattacaatca
ctccaactttccattcagcacttcctagatggcaaatgcataagatttgggggggggggggatacaaagcttccattaataacagggtacacaaaatggc
gtctccctgctggctgtgactgtaacttccaagactgaaggggaaacaaattaaatcatttatacagtgtcagtaaagtttattttgttcaacaaacatg
atagaaagggatctggatttatttcttagggtgacagttcccctttaatcataggctcagcctggacaagtgttgcatttaatagacatttaataaaatg
gactttcattggtgaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
5' EST: CX915728 [+]cgtccgcgtttccccaataacgtatggatcttcccctgaatggcgacggtgccggtgtcgatttaaataacagttatttacccggcagcattcagagccg
ttccttcccggcaatttgcctctggctcccgggtaatttgccggcacaaagagcggagaagcgtcgggcgccggcagcgggaatctgtttcggcttttga
gaaaaggaggaaaagttcagacttgtaaacgttaaatccaagatctgaaacagggacccccagagagagagcgtgacgcctcctgcccaacgccgctaac
gggggccgcctggctgccattttatcagtccagatggcagaaatgatactcggtgccaatggtatacagcactgccagccctcgttagcaaacgtgcgca
ccggaagtggggaagaagcagcagaagaagcagccggaaggtgttgctgccagggaacctgcgatgggcaagagggataaggcggacagaggagacaaaa
agaagttcaagcggcgtcactatgaggaagaggatgaagaggaagaggaggtgcccgggggcgaataccaggaagccgttccttctgccgcgggaaagca
agtggacgaagccggcacaaagcaggatgagtacggagccaaagactatcgcctgcagatgcctctganaatggatcattcctctcgccccctgtgggtg
gcacccgacgggcacatcttcctagaagccttctcgccagtgtacaagtacgctcaggatttcctggtggccatatcggaacccgtgtgcagaccc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: