XB-CLONE-3585429
Clone Name: IMAGE:7691191
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Gene:
pnpla6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC129023 [+]
GAACGCAGTGTAGCGAAGCGGAGAGAGAGGGACGCACAGCGCTGGGGCCGCAGGGGCTGCTGGGATTGGCTACATTGCGGGCCGCAGGGGCTGCTGGGAT
TGGCTACATTGCGGGCCGCAGGGGCTGCTGGGATTGGCTACATTGCGGGCCGCAGGGGCTGACGGGACCGGGGATATGGGTGAGGAGAACGGCAGCTGCA
ATGAGCCGGCCCTGTCCATGGGTATAGACCTGCGCACCCGCGTGCTCCAGTTCGTGGAAGACCGACTGCAGACCACCATGTGGACCGGAATCCTGATCGG
GGCCCTGGTCGCCATGGCCGTCATTGGGGTGATCGGCTACATTGTCTACAAGAAGTTCAGGCAGTCCAAGCAACCACATTCAGACGTGCCGCGCTATCGC
TTCCGGAAGAGAGACAAAATGATGTTTTATGGGCGCAAGATCATGAGAAAGGTCACCACCCTGCCCAACACTCTCGTGGGAAACGCAGTCTCCAGGCCCA
GAACCAGGAAAAAACCAAGAGTGCTGTCTCTGGCTAAAAGGATACTCCGCTTTAAGAAGGAATACCCCACTCTGCAGAAAAAGGAGCCGCCCCCTTCCCT
GCTGGAGGCGGACCTTACCGAGTTTGACGTGAAAAACTCCCATTTACCCTCGGAAGTTCTGTACATGCTGAAGAACGTCAGGGTGCTGGGGCACTTCGAG
AAGCCCCTGTTCCTAGAGCTGTGCAAGCACATGGTCTTCGTTCAGCTGCACGAGGGCGAGTACATCTTCCGGCCCGGCCAATTGGACAACAGCATTTACG
TGGTGCAAGACGGGACGTTGGAAGTGTGCATCCAGGAGAGCGACGGCGCCGACGTGGTGGTGAAGGAGGTGTTGGCCGGGGACAGCGTGCACAGCCTGCT
CAGCATCCTTGATGTCATCACCGGACACACAGCACCTTACAAAACGGTTTCAGCCAGAGCCGCCACAGCTTCCACCATCCTGCGACTCCCGGCCAATGCC
TTTCAGGACGTCTTTGCCAAATACCCAGAGACCCTAGTGAGGGTGGTGCAGATCATCATGGTCCGACTCCAGAGAGTGACGTTTCTAGCTCTGCATAACT
ACCTGGGTCTGACTACTGAGCTCTTCAACTCGGAGAGCCAGGCCATCCCGCTGCTGTCGGTGGCCAGCCTGACGGTGGGGGCCGGGGGCAATAAGGCTGT
AAGGAGGCAGGTATCCAGCATGTCGGCGGAGGAGGAACATGGAGAGAAATGTATCGAGCAGCAGCCAGAGTCTGACCAGGGCTCCCTAGACCCGAGTAAA
TTCTCCTCAACTGATGGAAATGGCCACAAGCGGAGCCGCTCCTCCTCCAGCATCGCCGAAATCCCAGGCAGTCCCAAGGCGGCTAAATCCGACATGGACA
TGGCGTACGAGCGAGCGCGAGTGAGCCTCACCCCCGACGACATGCCGCCGAGCCCCATTCTGAACAAGCCCATCCTGAAGAAGTCGGTGACTGTCACGGA
GACGCCGTCGGCCGTTTATCACTACAAGGAGGAAGATCTTGTCATTCCGGAATTCAGCGTCAACCGACACCTGAACGCCATGTTTGAGGCGGCCAAAAAG
GATCTGTCGACCCTCATCAAACTTCAGGACCCCTCTCTGCTGAATGGAAGGGTGACTTTACATCAAGTCAAGGGTGGAACCATTTTATCCAGACAGGGGG
ACCAGGACGTGAATATTCGCTTCGTGATCTCGGGGCTCCTCCACGTTTACCAGCGGAAAATCGACCGGGAGGAGGAGACCTGCCTCTTCATCACCCACCC
CGGGGAGTTGGTGGGGCAGCTGGCCGTTTTAACGGGGGAACCCCTCATCTTCACCATCAAAGCCAATCGCGACTGCACCTTCCTCTCCATCTCCAAGTGC
CACTTCTATGAGATTATGAGGGAGCAGCCCAGCGTGGTCCTTGGGGTGGCACACACTGTAGTTAAGAGGATGTCCCCCTTCGTCAGGCAAATCGACTTTG
CGTTGGATTGGATGGAAGTGGAGGCGGGACGGGCTGTTTACAGACAGGGGGACAAATCGGACTGTACCTACATTGTACTGAATGGGCGCCTTCGCTCGGT
TATCAGAAAGGATGATGGGAAAAAGCAGCTGACTGGGGAATACGGACGCGGAGACCTTATCGGGGTGGTAGAAGCCCTGACCCACCAGCCGAGGGCCACA
ACTGTACATGCCGTGCGAGACTCCGAGCTAGCCAAGTTACCCGAAGGGGCCCTGACCTCCATCAAACGCAAGTTCCCCCAGGTGGTGACCCGGCTCATCC
ATTTACTCGGGGAGAAGATTCTGGGCAGCCTGCAGCAGGTGAACGGCCCCCTTACAGGGCACGGCCTAGGTCTCCACACAACCGGCAACAAGTGGGATTC
CAGCAACCCAGCCAGTAACCTTTCCACCGTCGCCATCTTGCCTGTGTCCGAAGAGGTTCCTCTGACAGCCTTCGCACTTGAGCTGAGGCATGCCCTGGGC
GCTGTGGGCCCCACCCTGCTGCTCACCAGCGACAATATCAAGCAGCGCCTGGGCTCCGCCGCTCTGGACAGTGTCCATGAGTACAGACTGTCCAGCTGGT
TGGGGCAACAGGAGGACATTCACCGAATTGTTCTGTATCAGTCGGATTATTGCCTGACGCCATGGACCCAGCGCTGTATCCGGCAGGCCGACTGCATCCT
GATAGTGGGGCTGGGGGAACAGGAGCCCACCGTCGGAGAGCTGGAGAAGATGTTGGAGAACACGGCCGTCCGGGCCCAGAAGCAACTGGTTCTGCTCCAT
AAGGAAGACGGACCCCCTCCGTCCCGCACAGTCGAGTGGCTGAACATGAGAAGCTGGTGCTCGGGGCACTTACACCTCAGCTGCCCCCATCGGGTTTTCT
CCAAGAGGAGCCTGCCCAAGCTGATCGAGATGTACGAACGGGTTTCCCAGAAGCCCCCGGACCGCCACTGTGACTTCTCCCGACTCGCTCGCGTGCTGAC
CGGCAACGCCATCGCCTTAATCTTGGGAGGAGGAGGAGCCAGGGGCTGCTCCCAGGTCGGCATCATCAAGGCGCTCATCGAGTCGGGGATCCCCATCGAC
CTGGTGGGGGGCACGTCCATCGGAGCCTTCATGGGGGCCCTGTACGCCGAGGAGCGCAGCTTCAACCGCATGAAGATCCGGGCGCGGGAGTGGTGCATGG
ACATGACTTCAGTATGGAAGAAGGTTCTAGATCTGACGTACCCGACCACCTCCATGTTTTCCGGTGCGGCCTTCAACAAGAGCATCAGCAGCATCTTCAA
GGACAAACAGATAGAGGATCTATGGATTCCGTATTTTAACATCACCACTGACATCACCGCTTCGGCCATGCGAATTCACACCGACGGCTGCCTATGGAGG
TACGTACGTGCCAGTATGTCCCTATCGGGGTACATGCCTCCACTCTGTGACCCCAAGGATGGGCACCTCCTGATGGACGGGGGCTACATAAACAACCTGC
CAGCTGACGTCGCTCGGTCGTTAGGCGCCAAAGTGGTTCTGGCTATCGACGTGGGCAGCAGAGATGAAACGGACCTTACCAACTATGGCGACGCCCTCTC
CGGCTGGTGGCTGCTGTGGAAGCGCTGGAACCCGCTGGCCGATCGAGTCAAGGTCCTGAACATGGCGGAGATCCAGACCCGACTCGCCTACGTGTGCTGC
GTACGCCAACTGGAGATGGTCAAGAACGACGACTACTGCGAGTACATCCGCCCCCCCATCGATCGCTACGGCACCCTGGAATTCGGCAAGTTCGACGAGA
TCTGTGATGTGGGGTACCAGCACGGCAAGACCGTCTTCAACGTGTGGGGGCGCAGTGGGGTCATCGACAAGATGCTGAGGGACCGACAGGAGACCTGCAA
AACCAAAACCTCCGATATAAACACCTGCCCCAACGCCTCTTTCACCGACCTGGCCGAGATCGTGTCCCGCATCGAACCCGTGAAGACAGCGGTCGTGGAC
GATGAGTCCGACTATCAGACAGAATATGAAGACGAAGAGATCCCCGACTCCCAGAAGGAGGATTATCTCAACTTCCTGAGCGCCCAGAACAATGGCGATG
TCTCCGACAGTGATGAAGAAGGGGAAATGGGGGTGAGACAACGTCCTGCAGCAAAATGGCGGCAGGGAATGGACCGATCCCAGGAGTACAGCTGAACCCG
CCCCTGCCTAGGAGAGCGAGAGACGGCTCTCCACCTGTCCCCCCGCATCAGAACCTCCTATATCAACGGTTCTTCTGCTTTGTGTGGTTCTAGATATAGA
TCTTCCGGACTGTAGCGGTTTGTGGCCCGGCCACGTACAGACGCAATAAAGCCTGTGGCTCGCGAGGGCGCGTCACGCCAGAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX916220 [+]acgcagtgtagcgaagcggagagagagggacgcacagcgctggggccgcaggggctgctgggattggctacattgcgggccgcaggggctgctgggattg
gctacattgcgggccgcaggggctgctgggattggctacattgcgggccgcaggggctgacgggaccggggatatgggtgaggagaacggcagctgcaat
gagccggccctgtccatgggtatagacctgcgcacccgcgtgctccagttcgtggaagaccgactgcagaccaccatgtggaccggaatcctgatcgggg
ccctggtcgccatggccgtcattggggtgatcggctacattgtctacaagaagttcaggcagtccaagcaaccacattcagacgtgccgcgctatcgctt
ccggaagagagacaaaatgatgttttatgggcgcaagatcatgagaaaggtcaccaccctgcccaacactctcgtgggaaacgcagtctccaggcccaga
accaggaaaaaaccaagagtgctgtctctggctaaaaggatactccgctttaagaaggaataccccactctgcagaaaaaggagccgcccccttccctgc
tggaggcggaccttaccgagtttgacgtgaaaaactcccatttaccctcggaagttctgtacatgctgaagaacgtcagggtgctggggcacttcgagaa
gcccctgttcctagagctgtgcaagcacatggtcttcgttcagctgcacgagggcgagtacatcttccggcccggccaattggacaacagcatttacgtg
gtgcaagacgggacgttgggagtgtgcatcca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: