XB-CLONE-3584355
Clone Name: IMAGE:7691728
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Gene:
atxn7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154704 [+]
GCGTTGCGCTGCCCCAGGAGCCGGGAGCTGCCTCCGTCACACAGCCGGCTGACTGATGGCGGAAAGAATGTCGGAGAGGGCCGAGGATGACGTCACAGGG
GAGCAGCAGCAGCGCAGAGCGGCGGCGGGCAGGCAGCAACGGCGGCAGCAGCAGCAGGGAGAGAGCTCGTCGGCAGCAGCCATGGCCGCCGGAGGGGATC
GCACATCCCTGCCCAGTCCCGGAGCCATGCTGGGGCAGCCGTGGGCTCACTGGGTTGAGGCAGTGAAATTACACGGGCTGGAAGAACTGGAGGAAAGTTG
GAAGGAGTGCGATAAAAAACGGGAAAGCATGCGACTAAGCAGAGAAGATATGGCAATATTTGGCCTGTGCCCGGCCTACGATGAGTTCTATCTGGTGATG
TGCAGCCACTGTAATCAGGTCATAAAGCCACAGGCATTTCAATCTCATTATGAAAGAAGACACAGTTCCATAAACAAAACTCCCGTGACGCCGCCATCCT
CCTCAACGAACTCCCTCTATTCCACTGTGTCTTCAAAAACGAAACCAAGCGGTGGCGGCAGTTGCAGCAGCACGCGCCCGTCCTCTGGAAGTAGTGCCTC
GTCCAACTCCAAGCTGATGAAATCTCCCAAAGACAAGCTGCCAATCGGGGGAAGCAACAAATCAGTGCATCCTGTGCAGCACAGCAAAGCCCCCCAGGAA
AAAATAATGACACCATCTGTGAAAGTGGAAAAGATGCATCCGAACCCAAAGATAGATGGGGTGTTAAAATCTACTGCTGGTCCTGCCTGTTCTTCTACTG
TGAGCTCTTCAATAAAGACTGGCCTTAATTGTCCATCAATACCAAAGCCACCCTTGCCTTCCCCTGGACAGATACCAAATGGTAAAGGCATTCTCTCGAC
GACGCCGTTTTCGGAAAAGAAACCCGACGACACGAACAACAGGAAATATTTACATAAGAGATTGTCAGATCGAGAGTTTGACCCTGATATACACTGTGGT
GTTGTCGATGTAGAAACCAAGAAGCCCTGCACTAGATCCTTGACATGCAAGACACATTCCTTAGGCTACCGGCGAGCAGTACAAGGGCGCAAGAAACGTT
TCGACGTGTTGTTAGCAGAGCACAAAAACAAGACGAGGGAAAAGGAATTGCTTCGTCATTCTGAACATCAGCAGCAGGCGCCTGCGCTCAGGGAACCGCA
TCCCGTACCTTCAAAATCTTCCCAGGAACTGCACCAAAACTCTCATGGACTTACTTCTCCAGAAGCAAAGCCTTCAATAACCAATAAGGCCAAGCCTCAT
AACCCCAGTCTTCCAAGGCCTTCAGTCTACCCGGCAACGCACAGTGGAAATGCCCCTAGCGACTCCCCGTCTGTACATGAATCCCCCCAACCGGTAGTAT
CTGCTGCTGAGCCTACTTGCCGCTTTTCCAGTGATGAGGGAGAGGGGGACGAGAAGGAGGAGTCTGCTGAAAAACTGGACTGTCATTATTCTGATCACCA
CCCTCGACCAGCAGCTTATTGCACATTTGGTAGCAGGCAGATTGGAAGAGGATATTACGTGTTCGACAAGCGATGGGACCACATCCGATGTGCACTTAAC
TTCATGGTGGACAAGCATCTTAATTCGCAAATGTGGAGGAAAATCCCTCCGGCATCCAGCAACTCGTCAGCGCACGCTCCACACCGGACCAGCACAAATG
CAACGTCTGCGTCTCATAGCGTCAACAGCACTGGTTTCCTCTCGCCCTGCACGGTGACCTCCCCTCCTGCGTCTGCCACCTCTCCCTGTGCGTCCCTCGA
CAAAGTGTCGTCACACGGAACTACACTAAACGCTCACCCGGTGGGGGCGAGCGACTCCGTCAGTAGTATGCAATCCAGACAAGTGTCTTCTTCACCATCC
ACACCATCAGTGCTTTCCCCTTTACTTTCCCCCCATTCCAACAAGCCTCAAAAGTTAAAAACTGCTAAGTCCCTCAAGCCTAAAGAAACCCCAGCCAGCA
GCACAAACTGCAGCAGTGCCAGTAGCAACAGCAGCAGCAGCGGCGGCGGTGGCGGCTCAGGAAAGAAACGGAAAACTAGCTCCCCACCCCAGCTGCTAGC
TCACCCCCCTCATTCCGCAGAGTCGATAAGGAAAAACTGTGCACTGAACTCTGGGACCTACCACTCGCCGACGACGAATTCGTCCCAGGGCAGCTCGCAT
GGTATCGGCCTCAATTTTGTGAACAGTAAATCTAACTCTGTCAGTCTTAAACATGACCAATCAGGGAGGGGTCCTCCAACAGGGAACCCTGCAGAATCCA
TTAAGAGGATGAGCGTGGTCATGAACAGCAGTGACTCCACGCTGTCATTAGGGCCATTTGTTCATCAGGCTGGCGAGCAAACTGTGAACTCACAAAACAG
CTTCTCCCACTCGCACGCTTCCCTGGACAAATTAATGGGGAAGAAGCGGAGATGCTCGCCCGGCACCAGCAGCATCACCACCAATAACAGCAAATCCAGT
AAGGTTGCCAAACTGCCACCGGTGAACAATGTTCACGCGAAACACAACAATGTCATAGCAGGGACTCAGGCGCTGACGAATAATTCGCTTCGGCATCAGC
CAAAGGCACGTCCCTGACAGCTGGTTCCGCTCTCATGAGAAAAACAAACTGGACAGCTTTCACTACAATCAGAATCCTCCAGCTGGCTCCCCGGACCCCA
AGATGCCTTTGAGTTATCTCAACCTTTTTTTCCCCATGGTCCTCAGGAGCGGGAGTTTTTGTGAACTTGTCAGCTGGGAGAAGGAACTCCCCGAAGCCAT
GTGTGTAGCAGAGAATGCTAACCCTACGGACTCTCGAATGTTCAGTCCCACCGCCGCTTTTATCAGTGTTAACGTGGACTGACGCGCTCGCTACCGACCT
GCGACATCTCATTCGCATTAAAAGTTGCAAGCTTCAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX917353 [+]cgttgcgctgccccaggagccgggagctgcctccgtcacacagccggctgactgatggcggaaagaatgtcggagagggccgaggatgacgtcacagggg
agcagcagcagcgcagagcggcggcgggcaggcagcaacggcggcagcagcagcagggagagagctcgtcggcagcagccatggccgccggaggggatcg
cacatccctgcccagtcccggagccatgctggggcagccgtgggctcactgggttgaggcagtgaaattacacgggctggaagaactggaggaaagttgg
aaggagtgcgataaaaaacgggaaagcatgcgactaagcagagaagatatggcaatatttggcctgtgcccggcctacgatgagttctatctggtgatgt
gcagccactgtaatcaggtcataaagccacaggcatttcaatctcattatgaaagaagacacagttccataaacaaaactcccgtgacgccgccatcctc
ctcaacgaactccctctattccactgtgtcttcaaaaacgaaaccaagcggtggcggcagttgcagcagcacgcgcccgtcctctggaagtagtgcctcg
tccaactccaagctgatgaaatctcccaaagacaagctgccaatcgggggaagcaacaaatcagtgcatcctgtgcagcacagcaaagccccccaggaaa
aaataatgacaccatctgtgaaagtggaaaagatgcatccgaacccaaagatagatggngtgttaaaatctactgctggtcctgcctgttcttctactgt
gagctcttcaataaagactggcctttattgtccatcaata
3' EST: CX917352 [+]gaagcttgcaacttttaatgcgaatgagatgtcgcaggtcggtagcgagcgcgtcagtccacgttaacactgataaaagcggcggtgggactgaacattc
gagagtccgtagggttagcattctctgctacacacatggcttcggggagttccttctcccagctgacaagttcacaaaaactcccgctcctgaggaccat
ggggaaaaaaaggttgagataactcaaaggcatcttggggtccggggagccagctggaggattctgattgtagtgaaagctgtccagtttgtttttctca
tgagagcggaaccagctgtcagggacgtgcctttggctgatgccgaagcgaattattcgtcagcgcctgagtccctgctatgacattgttgtgtttcgcg
tgaacattgttcaccggtggcagtttggcaaccttactggatttgctgttattggtggtgatgctgctggtgccgggcgagcatctccgcttcttcccca
ttaatttgtccagggaagcgtgcgagtgggagaagctgttttgtgagttcacagtttgctcgccagcctgatgaacaaatggccctaatgacagcgtgga
gtcactgctgttcatgaccacgctcatcctcttaatggattctgcagggttccctgttggaggacccctccctgattggtcatgtttaagactgacagag
ttagatttactgttcacaaaattgaggccgataccatgcgagctgccctgggacgaattcgtcgtcggcgagtggtaggtcccagagttcagtgcacag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: