XB-CLONE-3584342
Clone Name: IMAGE:7691682
|
Gene:
stk10
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC123019 [+]
GGGCTGCGCAGCCTCACCTCCGCACTGTGGGGGGTAGCCCCATTAGTTTCCTGACGCTAGGAAAGTCCACTCGGCGAAAGGCAATGGCTTTCGCCAACTT
CCGCCGTATTTTGCGGCTTCCTAACTTTGAGAAAAAGCGGCTGAGGGAGTACGAGCACGTCCGGAGAGATTTGGACCCCAATCAGGTGTGGGAGATCATT
GGGGAGTTGGGCGACGGTGCCTTCGGCAAGGTGTACAAGGCCAAGAACAAGGAAACTGGCATTTTAGCAGCTGCCAAAGTCATTGAGACCAAGAATGAAG
AGGAGCTGGAGGACTACATGGTGGAAATTGAAATTTTGGCCACCTGCAACCACCACTTTATAGTCAAGTTGCTTGGCTCCTACTATTGGGAGGGGAAACT
TTGGATAATGATTGAGTTTTGCCCAGGAGGAGCCGTGGATGCAATAATGCTTGAGCTTGATCGTGGACTGAAAGAGCCAGAGATCAGAACTATCTGTAGA
CAGATGCTGGAAGGCTTAACATACCTCCACAGTATGAAAATGATTCACAGAGACCTGAAAGCGGGAAATGTGCTGCTAACACTGGATGGCGATATCAAGC
TCGCGGATTTTGGAGTGTCAGCTAAAAATGTCAAGACCCTGCAGAGAAGGGATTCATTTATAGGGACACCATACTGGATGGCTCCAGAAGTGGTGATGTG
CGAGACCATGAAGGATGCTCCATATGACTACAAAGCAGATATCTGGTCTCTAGGAATTACTCTCATAGAAATGGCTCAGATTGAGCCCCCTCACCATGAG
CTCAACCCCATGCGGGTTCTGCTAAAGATTGCCAAATCTGAGCCCCCTACCCTTTCTTCCCCCTCCAAGTGGTCTCCGGAGTTCCACAATTTCCTGAAGA
CGGCACTAGATAAGAATCCTGAAACCAGGCCAAGTGCTGCGCAGCTACTGGAGCACCCCTTTGTGAAGAAAGTATCTGGTAACAAACCTTTGCGGGACCT
GGTAGCAGAAGCCAAGGCAGAAGTGCTAGATGAAATCGAAGAAAACGGAGAAGTGGAGGAGGAAGAAGCCTCAGACACGCCTAGTTCAAACAAGAGTGTC
AGCCAAAGTGCACTTGGGGAAAAAGACAAACATACTGGAAAGGAACACGTGGGCAATGGGATAAAAGCAGAGCCACAGAACACTGATTCCCAAGCAGACA
TTCATTCACAGAAAAGAAACCATGAAGGGAAGAATTATCCTGAACACAATAGACATGATGCTGTGAATGGGAAGCCAGACATAATAATCTTGAACCCTGT
GAGCAGCAACCATGAACCAAAAAGAAACTCTGCAGCAGAGAGTTACAGAAATGAGGAGCACGGTTCTGCAGTGTCATCCAACCAGAGGCCAAAAAGCAGT
CAGTCTGACCGTCAGTCTGTGGAACTTGTGCCCAATGGAAGTTTTGATTCCCCCACACGTTACTTCACTGGCTGGTCCAAGAGGGATTCGGATTCTGGCA
GCAATTCAGCTTCTGAAAGCATGGACATCAGTATGAATTTATCTGCAGACCTGTCAATTAACAAAGAGACTGGCTCTCTGTCACTCAGGGAAAGTCGTTT
GCACAAAAAGACCTTGAAGCGAACAAGGAGGTTTGTTGTGGACGGAGTGGAAGTGAGTATTACTACCTCAAAGATCATCGGTGACGATGAGAAGAAAGAT
GAGGAGATGCGCTTCCTGAGACGCCAGGAGCTGCGTGAATTGAGATTACTACAGAAGGAGGAACACCGGAACCAGGCTCAGCTGACCTCTAAGCACTCAT
TCCAGATGGAACAGATGTTGCGTCGCTTTGAGCAAGAAATGAATAGTAAACGGAAGTTTTATGACTCAGAACTAGAAGCTCTTGAGCGGCATCAGAAACA
GCAGATTGAACGGATGGAGCAAGAACATGCATTGCGCAGACGAGATGAGGCCAGGCGCATCAGAACTGAGCAAGAACGAGACCATGTCAAATTCTTGGAG
CAGCTAAAGCTTAGGAAAAAGGAGTTGAAAGCACAAGTGGAGAAACTCCCCCGGCAGCAGCGCAGAGATGCAATGAAAGTGCAAATGGACGATTTTGCTC
AGAAGAAGCATATAGAGGAACAACAATTCTTAAATAAGCAGAAGGAGGATTTGACCCTGGCCCTGAGAGTGATTGTTCTGGAGAACAGGAAGGAGATCTA
TAACAAGGAGCGGGAATTTCTAAACAAAAAGCAGCAACTTCTTAGAGACAGAGAAGCGGTCATCTGGGATCTGGAGGAGAGACACTTGCAGGAGAGACAC
CAGCTAGTGAAGCAGCAGCTGAAGGACCAGTATTTCCTACAGCGTCATGAGCTGCTGAGGAAACATGAAAAGGAGCAAGAACAGATGCAGAGATACAACC
AGCGAATGATGGAGCAGCTTAAGTTGCGCCAGCAGCAAGAGAGGGCCCGTCTGCCAAAAAACCAAAAAGCAGAGGCAAAAACCCGTATGACTATGTTCAA
GAAGAGCCTCCATATCAGCCCAAGCGGCAGTGCTGCAGAGCAGCGGGAGAAGATAAAGCAGTTTTCTTTGCAAGAGGAAAAAAGACAGAAAGCAGAAAGG
CTGCAACAGCAGCAGAAGCACGAGCACCAGTTGCTGGAAATGCAAGCGGAGTGTGACTGCAACGTCCGAGATCTTCTGCAAATGCAGAATGAAAAGTGCC
ACCTGCTGGTAGAACACGAGACGCAGAAGCTGAAAACCTTGGATGAGCATCACATTCAGATGATTAGAGAATGGAGAGAAAATCTTAGACCACGAAAAAA
GGCTCTTGAGGATGAATTAGAACATAAGAAGGAAGAGCAAGAAATGTTCTTTCGGATGAATGAAGAAGTTGCAGGACATCCATTCCCATCCAACAAGCCA
GCCAAGTTCTATTCTTTCAGCTCTCCTGAGGCCTCCTAATTTCTGCAGCTATAAGACAACTGTACAAACTAAAGGCCAAAAGCCACTTGGCACAGAAAAC
TTTTCCTCACGTCTTTCCTCGTTGGTGCTTAGTGCGATTTCGAAGTGATGAAGGGTGGACAATACTAAGGAAGAGAGTCACTTTGATCTGAAGGCGGGGC
TATGAAGGTGGAGCTCTATGCACAGAAAAAAGAATCCTGCTGTTGGAATATGCCTTTATTTGTTGTCTGCCTAGAGAACAAACCTCTGTACTTTATTTAA
TGACTTCAGTGCTTACAGGCCAGCTACCAATCATTTCTATAGTGAAATTTATTATTTTGGTCATGCAGACAAGCAGTTCAGCATCAGATATTCTGTGCTC
AGGCTATGCTAGTATACAAATTAAAGGAATGCATTTCTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX917367 [+]gcgcagcctcacctccgcactgtggggggtagccccattagtttcctgacgctaggaaagtccactcggcgaaaggcaatggctttcgccaacttccgcc
gtattttgcggcttcctaactttgagaaaaagcggctgagggagtacgagcacgtccggagagatttggaccccaatcaggtgtgggagatcattgggga
gttgggcgacggtgccttcggcaaggtgtacaaggccaagaacaaggaaactggcattttagcagctgccaaagtcattgagaccaagaatgaagaggag
ctggaggactacatggtggaaattgaaattttggccacctgcaaccaccactttatagtcaagttgcttggctcctactattgggaggggaaactttgga
taatgattgagttttgcccaggaggagccgtggatgcaataatgcttgagcttgatcgtggactgaaagagccagagatcagaactatctgtagacagat
gctggaaggcttaacatacctccacagtatgaaaatgattcacagagacctgaaagcgggaaatgtgctgctaacactggatggcgatatcaagctcgcg
gattttggagtgtcagctaaaaatgtcaagaccctgcagagaagggattcatttatagggacaccatactggatggctccagaagtggtgatgtgcgaga
ccatgaaggatgctccatatgactacaaagcagatatctggtctctaggaattactctcatagaaatggctcagattgagccccctcaccatgagctcaa
ccccatgcgggttctgctaaagattgccaaatctgagccccctaccctttcttccccctccaagtgg
3' EST: CX917366 [+]aggaaaaaatgcattcctttaatttgtatactagcatagcctgagcacagaatatctgatgctgaactgcttgtctgcatgaccaaaataataaatttcc
ctataaaaatgattggtagctggcctgtaagccctgaagtcattaaataaagtacagaggtttgttctctaggcagacaacaaataaaggcatattccaa
cagcaggattcttttttctgtgcatagagctcccccttcatagccccgccttcaaatcaaagggactctcttccttagtattggccacccttcatcactt
cgaaatcgcactaagccccaacgaggaaagacgtgaggaaaagttttctgtgccaaggggcttttggcctttagtttgtacagttgtcttatagctgcag
aaattaggaggcctcaggagagctgaaaaaataaaacttggctggcttgttggatgggaatggatgtcctgcaacttcttcattcatccgaaagaacatt
tcttgcccttccttcttatggtccaattcatccccaagagccttttttcggggtctaagattttctctccattctctaatcatctgaatgggatgctcat
ccaaggttttcagcttctgcgtctcgtgttctaccagcaggtggcacttttcattctgcatttgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: