XB-CLONE-3584029
Clone Name: IMAGE:7691917
|
Gene:
eps8
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC121948 [+]
GGTGAAACCCTTCCTGTGAAATGGCTGGCCTCCCCAGCTGCATAGAGTAAGGATATGGGGGACCTGCACACCATTTTTGACCTCAGCAACGAGCCTTTCC
ATTAAATTTGGATCCTCAGAAACTAACCAAAATGAATGGGCAGATCTCAAGCAGTTATCGTGGGGGCTTTGGACCCGGCTCCCAGCATCCCAACGGCTAT
GACTCCCCACGTTCCGACAGACAATCCAGCAGCAAGATCAGCGCAAAGAGTATTTATGAGCAGAGAAAGAATTATGCTCGGGATACCGTCAGCAGCCTGT
CGGAGACCTCCCAGTATCACATCGAGCACTTGACCACGTTTGTGATGGACAGGAAAGACGGAGTTCTAACAGTAGACGATGGGATCCGAAAATTAAAGTT
ACTTGATGCCAAAGGAAAAGTGTGGACCCAGGACATGCTCCTTCAAGTTGATGACAAAGGTGTTAGTTTGTATGATGTAGACACAAAGAGTGAACTAGAG
AATTTCCCATTAAGTAGCGTCCAGCATTGCCAAGCAGTCATGAACGCCTGTCGCTACGACTCCATCCTTGCCCTTGTGTGCAAAGAGCCGGCGCAAAGCA
AAGCTGATCTCCACCTCTTCCAGTGTGATGAGATCAAGGCTTCCCTGGTGCAACGAGACATTGACAGCGCCATCAGCGACAGCAAAGGAGGGAAGCAGAA
AAAGCGCCCAGAGGCCCTCAGGGCAATTACTCGGAATGATGACAAAATCCCCCCACACCCCGGGGCACCGGCCCCCACGCCTCCTGGATCTGTTACACAA
GTGGATGTGCGCAGTCGTGTGGCGGCCTGGTCCTCATGGGCATCTGAGTACGGCGACTACGACCCATCCCGGCAGAATCTGGACCAGGACTCGACACCAG
AGATGTTGGAAGCCCGAATAGACAGAGATGTCCAAATCCTAAATCACATTTTAGACGACGTGGAGTTCTTCATCATGAAGCTGCAGAAGGCGGCGGAAGC
ATTTTCCGAGCTCTCCAAGCGGAAGAAAGGGAAGAAGAAGAAGGGGCCGGGGGAGGGGGTCCTTACCCTGCGGGCGAGACCGCCGCCTCAGGAGGAGTTT
GTCGACTGCTTTCAGAAGTTCAAGCACGGGATCAACCTTCTGGCAAAACTAAAGTCCCACATTCAGAACCCCAGCGCCTCCGAACTGGTTCATTTTCTAT
TCACTCCACTTCACATGATGGTAGAGGCCACGGGGGGCCCTGATCTGGCACGGACAGTACTAAGCCCCCTTATCACAAAGGACTCGCTGGATTTCCTCAA
CTTCATTCTCAACAAAGAGGAGAAACAATTATGGACATCGTTGGGCGATTCATGGAATAAATACAGGGCAGAATGGCCCAAAGAGCAGTTTATCCCCCCG
TATGTCCCGCGCTTCAGGAACGGCTGGGAGCCCCCACTCCTGGGCTGTGCGGCGGCGCCGAAGGAACAGGAACTCTCTTACTTGGCTGAGTCTGTGGCCA
ATGTTGCGGAGCTGCAGCGCAAACAGGAATTCAACAGCGTGCCAAATATGTATTCCCATGTGTCCCGGTACCCGCCGTCGGAGGGGAATTTTGAGGAGCG
CGACCAAGTGGAGGCCCAGAGATACGCCTTGTGCAAGTACGACTTTGTGGCCCGGAACTCAAACGAACTCTCTGTCCTTAAAGATGATGTCTTGGAGGTC
CTCGATGATAAGAAGCAGTGGTGGAAAGTTCGGAGCTCGAGTGGGGCAGTCGGTTTTGTGCCAAACAATATTTTGGTAGCAATGAAGCCACAAGATCAGT
CAGCAAAGCCAATGGAAGCCGCGTACACGCACACAATCCAGAAACAGAAGTCCGACCTTATACAAAGCCAAGCGGGTCCAATCCCAGCCGCCCCCTCTCC
ACCACCAAACCCAGCGCCAACGTATTCCCCACTCGCTAATTCCCAGCAACCAACGCTGCCCGTGGCCAAGCTCTTCACTGCCGGACCAATCAGTCGACAG
AGCAGTTTGTCCAGTGACAGCGGGGGAGGAAGTGTCCGAGAGCAACATGGCCTCCAGCAGGTTCCAGCTGACCGCAGGAAATCCCAGATGGAGGAGGTGC
AGGATGAGCTGATCCACAGACTGACCATTGGGCGCAGTGCGGCGCAGAAGAAGTTCACCGTGCCGCGGCTGAACGCGCCGGCTGTCCATATTTCGTACAG
TTCGCCGGCAGAGGATGTGAAATCCTGGTTACAGGCCAAAGGCTTCAATTCCGTAACCGTCGACAGCCTCGGGGTGCTCACAGGGGCCCAGCTTTTCTCT
CTCACCAAGGATGAACTGAAGACTGTGTGTCCGGAAGGCCCGAGGGTGTACAACCAAGTTACAGTGCAGAAGGCCGCCATAGAGGAACAAAGTGGGGGCT
CAGAGCTGCTAGAGATCATGAAGAAACGGCAGGAAAGGATAAACATGGCAGCCAGCGACTCCGGGGTGGAATCCTTCGATGAAGGGAACAGCCACTGACT
CCGGTGATTGGGTGGTTGAAGCCCCAATGGAGGGGGGATCGTGGGGCAAGGATTCTACCCGAATGTGCCCGGGGGTCCGACGGGCCCAGTGTGAGTGATG
CCGCTGTTTGATAAGACTTTTCTAGACTCTATTCTGCTTTATACTCTTTGTATGATTTGCCAGATGTTATTTAACCCCTTGTGGAACAGATGCCAATTGA
TATTTAAGGAGGGGCTCCTGACATGCCCACCGGATTGTACAGACGCAACAAAGAGGTGGATTTGGCTACTGTGGGGGCAAAAGGCTACTGGAGAGGGGGG
TGCTAGCACTCATGTTACAGCACAATTAGCCACTCCCCCCTGGCAGAGCGACTGCGCCCCCTATGGTGAGATGGCGCCACGTTGTGTGTGTAAATAGGAA
CATTATCAGTATTTTCCATGAGTTCTGCTCTGTGGAACAGTGGTTTCTACCAGGACCAACCTCTTTCTCCTTCTTTCTGTAAATAAAATTTAAAGCAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX917680 [+]gtgaaacccttcctgtgaaatggctggcctccccagctgcatagagtaaggatatgggggacctgcacaccatttttgacctcagcaacgagcctttcca
ttaaatttggatcctcagaaactaaccaaaatgaatgggcagatctcaagcagttatcgtgggggctttggacccggctcccagcatcccaacggctatg
actccccacgttccgacagacaatccagcagcaagatcagcgcaaagagtatttatgagcagagaaagaattatgctcgggataccgtcagcagcctgtc
ggagacctcccagtatcacatcgagcacttgaccacgtttgtgatggacaggaaagacggagttctaacagtagacgatgggatccgaaaattaaagtta
cttgatgccaaaggaaaagtgtggacccaggacatgctccttcaagttgatgacaaaggtgttagtttgtatgatgtagacacaaagagtgaactagaga
atttcccattaagtagcgtccagcattgccaagcagtcatgaacgcctgtcgctacgactccatccttgcccttgtgtgcaaagagccggcgcaaagcaa
agctgatctccacctcttccagtgtgatgagatcaaggcttccctggtgcaacgagacattgacagcgccatcagcgacagcaaaggagggaagcagaaa
aagcgcccagaggccctcagggcaattactcggaatgatgacaaaatccccccacaccccggggcaccggcccccacgcctcctggatctgttacacaag
tggatgtgcgcagtcgtgtggcggcctggtcctcatgggcatctgagtacggcgactacgacccatcccggcagaatc
3' EST: CX917679 [+]gctttaaattttatttacagaaagaaggagaaagaggttggtcctggtagaaaccactgttccacagagcagaactcatggaaaatactgataatgttcc
tatttacacacacaacgtggcgccatctcaccatagggggcgcagtcgctctgccaggggggagtggctaattgtgctgtaacatgagtgctagcacccc
cctctccagtagccttttgcccccacagtagccaaatccacctctttgttgcgtctgtacaatccggtgggcatgtcaggagcccctccttaaatatcaa
ttggcatctgttccacaaggggttaaataacatctggcaaatcatacaaagagtataaagcagaatagagtctagaaaagtcttatcaaacagcggcatc
actcacactgggcccgtcggacccccgggcacattcgggtagaatccttgccccacgatcccccctccattggggcttcaaccacccaatcaccggagtc
agtggctgttcccttcatcgaaggattccaccccggagtcgctggctgccatgtttatcctttcctgccgtttcttcatgatctctagcagctctgagcc
cccactttgttcctctatggcggccttctgcactgtaacttggttgtacaccctcgggccttccggacacacagtcttcagttcatccttg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: