XB-CLONE-3583086
Clone Name: IMAGE:7692334
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Gene:
apc
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166140 [+]
ggcggcatcagtgcagcagtagcgagtcccacagtaagaccaatagtaacagaaggagtacattagttaagcatttttttgctgctggataacaaactgc
tgtgtctgggggctcacggtgggatgaacaactcgctcaaaaattggggagagagagacaaagagagactgttccagcctgggggtagcccagcatggct
gctgcttcgtatgaccagttgttaaagcaagtggcggcactgaagatggagaatacaaaccttcgacaagaattagaagacaattccaatcatcttacaa
aactagagagtgaggcatcaaacatgaaggaggttctcaagcagctacaaggaagtatcgaggatgaggcaatggcttcttctggccagattgatctgct
ggagcgtcttaaagaactaaatttagacagcagtaacttccctgcaggcaagactaggccaaagatgtctatgcgttcttacggaagcagggaaggctct
ctctctggtcattcgggtgaatgcagtccggtccctgtgggttcctttcaaaggagaggccttttaaatggaagcagagaaagtactgggtatctggaag
aacttgagaaggaaaggttattacttattgctgagcatgaaaaggaagaaaaagaaaagcgctggtactatgcccaacttcagaaccttaccaagaggat
cgatagtctcccacttacagaaaatttttctctgcagactgatatgaccagaaggcagctagagtatgaggcacggcagatcagagctgcaatggaggag
cagttgggaacatgtcaggacatggagaagcgagtacagacaagagtgggcaaaatccagcaaatagaggaagatatattacgcattcggcagctattgc
agtcccaagcagcagaggcagcagagcggactccacagagcaagcatgatgcaggcagcagggatgccgacaagctgcccgatggacaggggacctcaga
aatcgctacatccagcaatgttggaagtggacagggttcatcaagtagagcagatcacgacacagccagtgttatgagctctaacagcacttactctgtt
ccgcgaaggctcactagtcatcttggtaccaaggtggaaatggtatattcacttctgtcaatgcttggtactcatgacaaggatgacatgtctcggactt
tattagccatgtcaagttctcaagatagctgcattgctatgcgccagtctgggtgtcttcctcttctcattcagctattgcatggcaacgataaagactc
tgtgctgctgggaaactcccgtggaagcaaagaggcccgtgctagggctagtgcagctctacataacatcattcactctcagcctgacgacaagcgaggg
agaagggaaatccgtgtccttcacctcttggagcagatccgagcttactgtgaaacgtgttgggaatggcaggaggcacatgagcaaggaatggaccagg
ataaaaacccaatgcctgccccagttgaccaccagatatgccctgctgtgtgtgtgctgatgaaattatcttttgatgaagaacacagacatgcaatgaa
tgaacttggtggactgcaggctattgctgagcttttgcaagtagattgtgaaatgtatggacttattaatgaccactatagtgttacattgcggcgatat
gcagggatggccctgacaaatctcactttcggagatgtagctaacaaggccacactgtgctcaatgaaaagctgcatgcgagctctcgtagctcagctca
aatctgaaagtgaagatttacaacaggttattgcaagtgttttaaggaatttatcctggagagcagatgtgaacagcaaaaaaaacccttcgagaggtgg
gaagcgtgaaggcccttatggaatgtgctctggatgttaagaaggagtccactctaaaaagtgtgctaagtgccttgtggaatttgtcagctcactgcac
tgagaataaagcagatatctgctctgttgatggggcattggcgtttctggtgggcacattaacttaccgaagtcaaaccaatactctggccatcattgag
agtggaggtggaatactacgtaatgtgtccagcttgattgctactaatgaagaccacaggcaaatcctaagggagaacaactgtttgcagaccttactac
agcacttgaaatcacacagtttgactattgtaagcaatgcctgtgggaccctgtggaatttatcagcacgaaatgcaaaagaccaagaggccctctggga
catgggagcagtcagcatgctcaaaaaccttattcattcaaagcacaaaatgatagctatgggcagtgctgcagctttaagaaacttaatggcaaacagg
cctgcaaaatacaaggatgccaatattatgtcccctggctcaagtgtgccatctcttcatgttcggaaacagaaagctttggaagcagaattagatgcac
agcatttgtctgaaacatttgacaatattgataatttaagccccaaaaccacccaccgcaataagcaacggcataagcagaacctgtgcagtgagtatgt
tctggattcaagcaggcatgatgattcgatttgccgctcagacaattttagccttggtaatttgactgttctttctccatatattaattcaactgtgcta
ccgggatcatcttcacctcgaccaactatggaaggtaacaggcctgaaaaagacagagaaagaacagcaggacttagcaactaccattctgcctctgaaa
attcaggaaactcctcgaaacgtattggtgtacagttgtcaactacagctcagatttctaaagttatggatgaggtatcaaatattcacatggttcagga
agacagaagttcagggtctgcttctgaaatgcactgcatgtcagatgaaagaacttcacaaaggaaaccttctagtaatcaccctcattcaaatccattc
agttttgctaaaggagatagttccaacagggggtgccctgtagcatttatgaagatggaatacaaaatggcatctaatgacagtttaaacagtgtcagca
gcacagatgggtatgggaaacgtggccaagtgaagccatctgtagagtcttactctgaagatgatgaaggtaaatttggtagttatggacactatcctgc
cggcctagcacacaaaattcacagtgcaaatcacatggatgataacgatacagaactagatacaccaataaattatagtctgaaatattcagatgagcaa
ttgaactctggtagacaaagcccatcacagaatgagaggtgggcaagaccaaagcacatcatagacagtgaaatgaaacaaagtgagcagaggcagccta
gaaccaccaaaacaacatatagttcatatactgaaaataaagaaaaaaaaaaaaaaaaa
5' EST: CX918640 [+]gcggcatcagtgcagcagtagcgagtcccacagtaagaccaatagtaacagaaggagtacattagttaagcatttttttgctgctggataacaaactgct
gtgtctgggggctcacggtgggatgaacaactcgctcaaaaattggggagagagagacaaagagagactgttccagcctgggggtagcccagcatggctg
ctgcttcgtatgaccagttgttaaagcaagtggcggcactgaagatggagaatacaaaccttcgacaagaattagaagacaattccaatcatcttacaaa
actagagagtgaggcatcaaacatgaaggaggttctcaagcagctacaaggaagtatcgaggatgaggcaatggcttcttctggccagattgatctgctg
gagcgtcttaaagaactaaatttagacagcagtaacttccctgcaggcaagactaggccaaagatgtctatgcgttcttacggaagcagggaaggctctc
tctctggtcattcgggtgaatgcagtccggtccctgtgggttcctttcaaaggagaggccttttaaatggaagcagagaaagtactgggtatctggaaga
acttgagaaggaaaggttattacttattgctgagcatgaaaaggaagaaaaagaaaagcgctggtactatgcccaacttcagaaccttaccaagaggatc
gatagtctcccacttacagaaaatttttctctgcagactgatatgaccagaaggcagctagagtatgaggcacggcagatcagagctgcaatggaggagc
agttgggaacatgtcaggacatgga
3' EST: CX918639 [+]ctttattttcagtatatgaactatatgttgttttggtggttctaggctgcctctgctcactttgtttcatttcactgtctatgatgtgctttggtcttgc
ccacctctcattctgtgatgggctttgtctaccagagttcaattgctcatctgaatatttcagactataatttattggtgtatctagttctgtatcgtta
tcatccatgtgatttgcactgtgaattttgtgtgctaggccggcaggatagtgtccataactaccaaatttaccttcatcatcttcagagtaagactcta
cagatggcttcacttggccacgtttcccatacccatctgtgctgctgacactgtttaaactgtcattagatgccattttgtattccatcttcataaatgc
tacagggcaccccctgttggaactatctcctttagcaaaactgaatggatttgaatgagggtgattactagaaggtttcctttgtgaagttctttcatct
gacatgcagtgcatttcagaagcagaccctgaacttctgtcttcctgaaccatgtgaatatttgatacctcatccataactttagaaatctgagctgtag
ttgacaactgtacaccaatacgtttcgaggagtttcctgaattttcagaggcagaatggtagttgctaagtcctgctgttctttctctgtctttttcagg
cctgttaccttccatagttggtcgaggtgaagatgatcccggtagcacagttgaattaatatatggagaaagaacagtcaaattacaaggctaanattgt
ctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: