XB-CLONE-3582084
Clone Name: IMAGE:7693145
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Gene:
rfx3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121500 [+]
CACCTTCTCTCCACCCTCATCATCATCATCATCCCCATCACCCCAACAACAACAACCAACAACAACATCACCACCACAATAAGCCACAGCCAAGAGACTA
CAATGCAGACGTCCGAAACGGTGTCGGACACGGGTTCGACAGTGACTTTGCAGACATCTGTGGCAGGTCAAGCAGCGGTTCCTACACAGGTGGTACAGCA
GGTTCCAGTACAGCAACAGGTTCAGCAGGTACAGACTGTTCAGCAGGTGCAGCATGTTTACCCAGCACAAGTACAATATGTTGAAGGAAGTGACACAGTC
TACACAAATGGAGCAATCCGGACAACAACATATCCTTACACAGAAACCCAGATGTACAGCCAAAATACTGGTGGGAATTACTTTGATACCCAGGGTGGAT
CAGCACAAGTGACAACAGTGGTCTCAACGCACAGCATGGTGGGAACTGGAGGAATTCAAATGGGTGTCACTGGAGGACAAATCATTAGTAGCACTGGTGG
AACTTACCTGATTGGCAATGCAATGGAAAACTCAGGCCATTCTGTGAGTCATACAACTCGAGCCTCTCCAGCAACAATTGAAATGGCGATCGAGACACTG
CAAAAGTCTGACGGTCTGTCGAGTCACAGGAGCTCTCTTCTCAACAGCCATCTCCAATGGCTGTTGGATAACTATGAGACGGCAGAAGGAGTGAGTCTCC
CTAGAAGCACTCTCTACAATCATTACCTAAGACATTGTCAAGAGCACAAACTGGATCCAGTCAATGCTGCATCTTTTGGAAAACTCATACGCTCAATATT
CATGGGATTACGTACTCGAAGACTAGGGACCAGGGGTAACTCCAAATACCATTACTATGGGATTCGTGTCAAGCCAGACTCCCCTCTTAATAGGCTACAA
GAGGACATGCAATACATGGCTATGAGACAGCAACCCATGCAGCAGAAACAAAGGTACAAGCCTATGCAGAAAGTGGATGGAGTGGGAGATGGATTTGCAG
GCAGTGGGCAGTCTGGTGCCTCTGTTGAGCAAACTGTGATTGCCCAAAGCCAGCACCATCAACAGTTTTTAGATGCGTCTCGAGCTCTTCCAGAGTTTGC
AGAAGTTGAAATATCTTCTCTGCCAGATGGTACAACCTTTGAAGATATCAAGTCGTTGCAGAGTCTTTATCGAGAGCACTGTGAGGCGATATTGGACGTT
GTTGTTAATCTACAGTTTAGCCTGATCGAGAAGTTGTGGCAGACTTTCTGGCGTTACTCTCCTTCATCTCCAGCCGATGGTTCCACCATTACAGAATCAG
GAAACCTGAGTGAAATAGAAAGCAGGCTGCCCAAGTCAAAGCTGATTTTGTTCTGCAAAAACGAGTCTATTGTAAAGTGGATGTGCAACTGTGACCATAT
GATGTACCAATCCTTGGTGGAGATTCTCATTCCTGATGTCCTAAGACCAATTCCTAGTGCCTTGACCCAAGCCGTTCGCAATTTTGCAAAGAGCCTTGAA
GGTTGGCTCTCTAGTGCCATGAGTAATATTCCACAAAGAATGATACAAACCAAGGTTGCTGCTGTAAGTGCCTTTGCCCAAACTCTGCGTAGATACACAT
CCCTTAATCACTTGGCTCAAGCAGCTCGCGCTGTGCTGCAGAACACGTCTCAGATCAACCAGATGCTAAATGACCTCAACCGTGTGGACTTCGCCAACGT
CCAGGAGCAGGCTTCCTGGGTGTGTCAGTGTGATGACAGCATGGTGCAGAGACTAGAAACAGACTTTAAGATGACTCTCCAGCAGCAGAGCACTCTGGAG
CAGTGGGCTGCCTGGTTAGACAATGTGGTGACACAAGCCTTGAAACCGTATGAAGGACGGCCCAGCTTTCCTAAAGCAGCCCGGCAATTTCTGCTCAAGT
GGTCATTTTACAGTTCTATGGTTATTCGAGACTTAACCTTGCGCAGTGCTGCCAGCTTTGGTTCTTTCCACCTGATCCGCCTTCTCTACGATGAATACAT
GTTTTATTTAGTTGAGCACAGAGTTGCCCAGGCAACTGGAGAATCACCAATTGCAGTCATGGGAGAGTTTGGAGATTTAAATGATGCATCACCAGGAAAT
ATGGAAAAAGATGAAGGCAGCGATGTGGAGAGTGAGATAGAGGAAGAGCTTGATGATTCTGTAGAGCCCCCAGCCAAGAGGGAAAAGACGGAGCTGAGCC
AAGCGTTCCAGGTGGGCTGCATGCAGCCATCGCTGGAGGGGAGCGTTCAGCAAAGCCTGGTCTTAAATCAGCTGCACAGCGAGCACATTGTTACAAGCAC
TCAGACCATCAGGCAGTGCAGTGCCACCGGGAATACCTACACTGCCGTCTAGCGCAAACAAAAGCTTGCAGAAATGAGATGTAGAAATATATCTATGTCG
GGCTTAAATGAGTGGCGGCAGAACAGAATATGACTGAAATGTGACTGTTTCTGGCCTTTCAGACCCACCTTTTTTATCTTTACCCCCCCCCCCCAAGCAT
TTGCCCCTCCAACTCTTGCATTGTAAAGTAGATTTTCCAGGCAGTTCATGGATTTTATTATGTTTTAATATACCCAAACCTTTCTTTCATTTTAGAAAAA
AAAAAAAAAAAAA
5' EST: CX919671 [+]caccttctctccaccctcatcatcatcatcatccccatcaccccaacaacaacaaccaacaacaacatcaccaccacaataagccacagccaagagacta
caatgcagacgtccgaaacggtgtcggacacgggttcgacagtgactttgcagacatctgtggcaggtcaagcagcggttcctacacaggtggtacagca
ggttccagtacagcaacaggttcagcaggtacagactgttcagcaggtgcagcatgtttacccagcacaagtacaatatgttgaaggaagtgacacagtc
tacacaaatggagcaatccggacaacaacatatccttacacagaaacccagatgtacagccaaaatactggtgggaattactttgatacccagggtggat
cagcacaagtgacaacagtggtctcaacgcacagcatggtgggaactggaggaattcaaatgggtgtcactggaggacaaatcattagtagcactggtgg
aacttacctgattggcaatgcaatggaaaactcaggccattctgtgagtcatacaactcgagcctctccagcaacaattgaaatggcgatcgagacactg
caaaagtctgacggtctgtcgagtcacaggagctctcttctcaacagccatctccaatggctgttggataactatgagacggcagaaggagtgagtctcc
ctagaagcactctctacaatcattacctaagacattgtcaagagcacaaactggatccagtcaatgctgcatcttttggaaaactcatacgctcaatatt
catgggattacgtactcgaagactagggaccaggggtaactccaaataccattactatgggattcgtgtncagccagactcccctcttaataggct
3' EST: CX919670 [+]ctaaaatgaaagaaaggtttgggtatattaaaacataataaaatccatgaactgcctggaaaatctactttacaatgcaagagttggaggggcaaatgct
tggggggggggggtaaagataaaaaaggtgggtctgaaaggccagaaacagtcacatttcagtcatattctgttctgccgccactcatttaagcccgaca
tagatatatttctacatctcatttctgcaagcttttgtttgcgctagacggcagtgtaggtattcccggtggcactgcactgcctgatggtctgagtgct
tgtaacaatgtgctcgctgtgcagctgatttaagaccaggctttgctgaacgctcccctccagcgatggctgcatgcagcccacctggaacgcttggctc
agctccgtcttttccctcttggctgggggctctacagaatcatcaagctcttcctctatctcactctccacatcgctgccttcatctttttccatatttc
ctggggatgcatcatttaaatctccaaactctcccatgactgcaattggtgattctccagttgcctgggcaactctgtgctcaactaaataaaacatgta
ttcatcgtagagaaggcggatcaggtggaaagaaccaaagctggcagcactgcgcaaggttaagtctcgaataaccatagaactgtaaaatgaccacttg
agcagaaattgccgggctgctttangaaagctgggccgtccttcatacggtttcaaggcttgtgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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