XB-CLONE-3580517
Clone Name: IMAGE:7694085
|
Gene:
adamtsl5
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC121503 [+]
GGAACAGAAACACACGCAGAGGCTTCACCTGGGACACAAAGTTGTACGGGGATTAGTGGCAGCTGCCAAAGCATGGGCTAAGGGCACAACTCCCACTCAC
CCTGCCCCTACTGGTATTTACTAACCATGGCTGTGTGTGCAGTAAGCGCTGGAACAGAACGTAGACCCACTGGTCACCATACCTGTGGCTTAATTCCTGG
TGGATCCTAGACTTTGCTCGCCTTCATTGACCCTTCTCCCACCACTTTCTTCCTTGGTTTCATGTAATTAACTCTTTGCACCACTAACGGAGGAGGATGC
TTTGGACTTGCAGGTTAATATGTCACTGTTTGGCTACTGCTTGTGTCCTTTGGATCTTCAAGATTGTTCCATCTTCCAGCCAGGTACAGAGGAACCCCTT
TCAGTGGGGATTTGTCAACAGATTTGGCCCTGAGCACAGTCCACCCCTAAGCTCTAGAAATGAGTGGAGTGTGTGGCACAGTTGGTCACCATGTTCTCGA
ACCTGTGGTGGAGGTGCTGCTGTACGCACCAGGACATGCCTAATCAGGGACCAAGGAAGCCCAATGTGCCCAGGCGAAATGAGACAATACCAAGTATGCA
ACACACAGAACCTAATCAAACTGTCTAACCCAGGACTGCCCTTCAGGAGTTGCAGATTTCAGGCATTTCCAGTGTTCTGCATACAACAAGAAGCCTTTGA
TGGGAAATTACTTCAGATGGGTTCCTTTCTACTCTGGTCAAAGTGATTGTGAGCTCAGCTGTTTGGCAGAAGGACAGAATTTTTATTATAACTTTGGTCG
AGTTTTAGATGGAACATCATGCCATTCAGACTATGATGGCTTATGCATCAATGGGCAGTGCCTGAAAATTGGCTGTGATCTTATCCTGGGATCAGAGGAG
AAGACAGACATTTGTGGAGTTTGTGGTGGGAAAAACATCTCATGCAGGCATTACCATAATGTATACACTACCAAGTACCCACCGTCTGGACTCTTTGGAT
ACAATGAAGTGACCATGATCCCCACAGGAGCCACTAACATCAAGGTGACTGACAGAAGTAACAATTATTTGGCCCTAAGAAACAGAAATTACCACTATGT
CATCAATGGCAACTGGGCCATTAGCTATCCTGGTGTCTACAATGTGGCAGGGACCAAAGTCCACTATAAACGTGCTGCTGATAATCAAGAGACTTTTGAG
TCACAAGGACCCACCAGTGAGGATCTACACATTATGGTACTTTTTACTGAGCAGAATTTGGGGATTGAGTATGAATACTGGCTTCCCAAGGATTATTATA
TACAGCACCAAAGAGACCGCAGCTCTCTGCAAGAATATGGAGAGGTCTCCGTGCCTCCTACCCATCCCTCTACCACACAGCCAACACCAAAGACCACTGC
ACAAATTCTAACACATCACATCAAGAAGCAGCCCCAGGCACGTCTCCAAAGGGAGAACACTGAGTGGAATCAAGTTAAGGAATCTATAGAGAACAGTAAA
TGTGGAAAGTGCAAAAAAGTGAAAGGACGACAAAATAAAGTGAAGCAATATTGTCAGAAGGACTTTGTTTTCAGAGGAAGAGTTTTAAGCAAAAAGACAG
TTGGGAAGGAAACGTGTTACGATATTCAAGTGGTTATGACCTACAAAAACAACTTTCCTATTCTGAGACGGGAATACATTTGGGTTCCGAACACTTGTGA
TTGCCCTTACATGTCGGAAAAAAGGGAATATATTATAATGGCACGGAGACATGTCAACTATGAGCACACATTGAACAGGATTTTGCTGGAGACCCATAGT
TTTGTTCGCCCCTACAGTCCAAAGGAGGACAAGATGCTCAGAGACCTTGACAAAGAATGTGCCAAATATGGAATCCAAAGTACAAAACAGAATCATTAAC
TGTTTAGAACCAAGGAAAACATTGTATTTCCTGTAGTTCAGTATAACTATCTACAGATGGCTTTGTACAGTGTATTTAATATTTTATTGCACAGTGTGTA
AACGTATATAAAGTGTGTAGTTGGAGATACTATACACTTTAGAAGGGTTGCAGAGTATTGAAGCTGGTTATGCATGATGAGATGCAGATGGAAGAACTTA
TTGACTGCTGCTCTGTGTGGAAAGCCCTTCACTATTTATTTTGCCTTCCTGCAATTATATCATAGTCTGGTCTGCTGATGCCTCCCATGAACAAATGTTA
GAGAATTGGCTAACCTGGAAGTGACATTGTCTGCAGCGGCCTTCCATATCTTATGGCATCATTAGATCCTGTATCATATGAGTGAGCCAGTTCAGCCTGG
CCACCCATATTTCACATCTATTCATATTGCCACAGTTTAGATATGTGGATCACATGAGAATCCACCTATCTGTGATTCCTTTGGTTGTTGTAAATTACAG
ATACTCTCTTAACCTGTAACCGAGTCTCTTCTTATTTTTTTTCCTGCTGCTTTCTTTTAGTTGAAATCACAATTTTTACTCAAAGAAACACTATTTCTGT
ATACAGAAAACTTCCTAGTAACAAAATATATCTGTCAGTGTTAGGCTATTTACACACAGAGCATGTACATATGGTTGTATGGTTCAATTGAAATATGCGT
GTATAAGCACATATGAATTTTATACCACTCACCTTTTTATACCACTTACCATGCACTTACAATGCATTTGCCAATAAAAAATGATGCATTCCAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX921306 [+]gaacagaaacacacgcagaggcttcacctgggacacaaagttgtacggggattagtggcagctgccaaagcatgggctaagggcacaactcccactcacc
ctgcccctactggtatttactaaccatggctgtgtgtgcagtaagcgctggaacagaacgtagacccactggtcaccatacctgtggcttaattcctggt
ggatcctagactttgctcgccttcattgacccttctcccaccactttcttccttggtttcatgtaattaactctttgcaccactaacggaggaggatgct
ttggacttgcaggttaatatgtcactgtttggctactgcttgtgtcctttggatcttcaagattgttccatcttccagccaggtacagaggaaccccttt
cagtggggatttgtcaacagatttggccctgagcacagtccacccctaagctctagaaatgagtggagtgtgtggcacagttggtcaccatgttctcgaa
cctgtggtggaggtgctgctgtacgcaccaggacatgcctaatcagggaccaaggaagcccaatgtgcccaggcgaaatgagacaataccaagtatgcaa
cacacagaacctaatcaaactgtctaacccaggactgcccttcaggagttgcagatttcaggcatttccagtgttctgcatacaacaagaagcctttgat
gggaaattacttcagatgggttcctttctactctggtcaaagtgattgtgagctcagctgtttg
3' EST: CX921305 [+]ggaatgcatcattttttattggcaaatgcattgtaagtgcatggtaagtggtataaaaaggtgagtggtataaaattcatatgtgcttatacacgcatat
ttcaattgaaccatacaaccatatgtacatgctctgtgtgtaaatagcctaacactgacagatatattttgttactaggaagttttctgtatacagaaat
agtgtttctttgagtaaaaattgtgatttcaactaaaagaaagcagcaggaaaaaaaataagaagagactcggttacaggttaagagagtatctgtaatt
tacaacaaccaaaggaatcacagataggtggattctcatgtgatccacatatctaaactgtggcaatatgaatagatgtgaaatatgggtggccaggctg
aactggctcactcatatgatacaggatctaatgatgccataagatatggaaggccgctgcagacaatgtcacttccaggttagccaattctctaacattt
gttcatgggaggcatcagcagaccagactatgatataattgcaggaaggcaaaataaatagtgaagggctttccacacagagcagcagtcaataagttct
tccatctgcatctcatcatgcataaccagcttcaatactctgcaacccttctaaagtgtatagtat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: