XB-CLONE-3580469
Clone Name: IMAGE:7693996
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Gene:
ppp6r3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121957 [+]
GGAGGAGGGGAGGGGTTGAAGAGTTGATATGTCCACCATCTTGAAAACTCGCTTATACTGCTGAGCAATTTTCCCGCCTGCCTGAATAAGGTTGTTGGTC
ACTTCAGGAATAAAATTGACCGTTTAACCGAATTTAAGAGACAGATTCAAAACAACCTGATGTAGGCAGGATAACCCTGTAATGGGCCAGGAGTTGAAAA
AGCTTATAGTTTTATTTTCTTAATTTTTTTTAAATTATTTTAACAGTCAAGTATGTTTTGGAAATTCGACCTACATTCATCATCTCACATAGACACTCTT
TTGGAAAGAGATGAGGTGACGCTAAAGGAATTGATGGATGAGGAGGATATATTGCAAGAATGCAAGGCCCAGAATCGAAAGCTTGTGGAGTTTTTATTAA
AAGCTGAATGCTTAGAAGACTTGGTGACTTTCATAACTGAGGAACCACCCCAAGACATGGATGAGAAGATAAGATACAAGTATCCTAATTTGTCTTGTGA
GCTGCTAACCTCTGATGTGTCCCAGATAAATGACCGACTAGGAGAAGATGAAGCTTTACTCAAGAGACTATATGCCTTTCTTTTGAATGATCCTCCATTG
AATCCTTTGCTTGCCAGCTTCTTCAGCAAAGTGTTAAGCATTTTAATCAGCCGGAAACCAGAACAGATTGTGGAATTCTTAAAGAAAAAAGATGACTTTG
TCAACCTGATCATAAAACACATAGGAACCTCTGCAATTATGGACTTGCTGCTACGACTTCTCACGTGTATCGAGCCACCACAACTCAGACAAGATGTCCT
AAATTGGTTAAATGAAGAGAAGATTATTCAAAGGCTTGTAGAAATTGTTCACCCGTCACAGGATGAAGATAGGCACTCAAATGCTTCACAATCACTTTGT
GAGATTATACGTCTGAGCAGAGATCAGATGCTACAAGTACAAAACAGCTCCGAGCCAGATCCACTGCTCGCCACTCTGGAAAAGAAAGAAATTTTAGAGC
AGCTTTTATCAAATATATTTTTGAAAGAGAAAAATGAATCTGCGATAGTCAGTGCAATACAGATTTTGCTGACCTTATTAGAAACAAGAAGACCGGCATT
TGAAGGGCATATAGACCTTTGTCCTCCTGGAGTGAACCATTCTGCGTTTTCAGTCAATAAAAATGTATTGGCAGCCATTCAAGAGAGACTTCCATCTTTT
CACCAGCTCCTTCTGGACCCTCCAAAGACTGGTGTAATGAAGACAACTTGGGGAATTCTTGATCCTCCTGTAGGGAATACCAGGTTAAATGTGATAAGGC
TAATAGCGAGTCTACTCCAGACCAATACACATTCAATCAACGAGGAGCTTATTGAGCTGAACACAATGGGAGTTATACTGGACATGTTTTTTAAATACTC
TTGGAACAATTTTCTTCACACCCAAGTAGAAATCTGCATAGCATTGATTCTTGCTAGCCCTGGTGACTCTCCAGAGCACAGCACGATTAGTGAGCAGAAC
TCCCCTGCAGACCATGTTCTTTTAAAGCATCTATTCCTGAAATGCCACTTAATAGATCGAATACTTGAAGCTTGGGCTATGAATGAAAAAAGACAGTCTG
AAGGAGGACAGAGGTACGGCTACATGGGTCACTTAACACGAATAGCAAACTGCATTGTCCATAGCATAGAGAAAGGGCCAAACAGTGTTCTAGCCCACCA
GCTTATCAAAGATTTGCCAACTGATGCCCAGGAAAGATGGGAAACATTTTGCACAAGTTCACTGGGAGAAACAAACAAGAGAAACACAGTGGACCTGGTT
ACAACAAGACATATCCACTCCTCCAGCGATGATGAAATTGAGTTTAAAGACCCTGGTTTCTCACAAGATTCTGCCATACAGCAGTTTGGCTTCAACGATG
AGAAGTTTGCTGACCAAGATGACATCGGGAACGTCTCCTTTGATCGAGTGTCAGACATCAACTTCTCCCTCAATGCAAATGAAAGTGCTAATATAGCTCT
GTTTGAGGCCTGCTGCAAGGAGAGAATACAACAGTTTGATGATGGTGGATCAGATGAGGAAGACATCTGGGAAGAGAAAAATATTGCCTTCCCCCAAGAG
ACACAAAGGAGGTCAAGCTCAGGAAGCACAGACAGTGAAGAGAGCACAGATTCTGAAGAGGAAGAAACTGTTAAACAGGGATTATTTGAGTCAAGCACAG
TCAACCACGAAGACAAAATGGAAGTCGATGCTAATGAAGCAAGCAACTGGACTGCAAATTTTGACATCCCAATGGAAACTGCACACGTGGCAAACCTGGA
CTCTGTTGGTTCTGATGTATGGAGCACAGAAGAGCCCTTGTCTTCTAAAGAAAGTGGCTGGGCCTCTTTTTCTGAATTCACATCTCAAATTAGTACAAAG
GAGACAGTAAGAAGTAGCTCCCCTGTTGAAATGGAAACAAGCACAGAGCCAGTGGATCCTCTCAGTGTAAATGCATCAGCACAAACAGAAGTTACTGTTG
CTATGGATGCAGTTTCTGATGGGGAAGAGGAGGTGGAAAATGCAGATCAGATGACAGAAACTGTGATGAATGGCAGCATGAAGGAAACGCTCAGCCTTAC
CGTAGATGCAAAAACAGAAACCGCTGTTTTTAAAAGTGAGGAAGGGAAACTGACTACCTCTCATGATTCAGCTTGTAAATATGGTGTAGTGGAAAACTTT
GACACAGCAATGGAAAATCCTCCTCCTTCACAGCCCAGTGGTAGCAGTCAAGAACAGAGAACCTCTGAACAAATTGTGGTAGACGGAGCTTCAGCAAATG
GGCCTGTATGATGTGATACCTGTTGCTAAGTTGAAGACTGCTGACTGATATCCACAGGGGATTTAAATAGCGTACTTGAGGATTTTGGGAAAAAAATGCC
TGTCAAAGCCAGTCACTGCTGCACTAACTTTGCAAGGGATCAGGACCAGCAACATTTTTTTTTTTTCCCTTTTTGATTTTAAGGTGTTGTACAGTGTAAA
AAAACAAAAACAAAAAAAACCAATTGCACATTGACAAAAAAAAAAAAAAA
5' EST: CX921351 [+]gaggaggggaggggttgaagagttgatatgtccaccatcttgaaaactcgcttatactgctgagcaattttcccgcctgcctgaataaggttgttggtca
cttcaggaataaaattgaccgtttaaccgaatttaagagacagattcaaaacaacctgatgtaggcaggataaccctgtaatgggccaggagttgaaaaa
gcttatagttttattttcttaattttttttaaattattttaacagtcaagtatgttttggaaattcgacctacattcatcatctcacatagacactcttt
tggaaagagatgaggtgacgctaaaggaattgatggatgaggaggatatattgcaagaatgcaaggcccagaatcgaaagcttgtggagtttttattaaa
agctgaatgcttagaagacttggtgactttcataactgaggaaccaccccaagacatggatgagaagataagatacaagtatcctaatttgtcttgtgag
ctgctaacctctgatgtgtcccagataaatgaccgactaggagaagatgaagctttactcaagagactatatgcctttcttttgaatgatcctccattga
atcctttgcttgccagcttcttcagcaaagtgttaagcattttaatcagccggaaaccagaacagattgtggaattcttaaagaaaaaagatgactttgt
caacctgatcataaaacacataggaacctct
3' EST: CX921350 [+]gtcaatgtgcaattggttttttttgtttttgtttttttacactgtacaacaccttaaaatcaaaaagggaaaaaaaaaaaatgttgctggtcctgatccc
ttgcaaagttagtgcagcagtgactggctttgacaggcatttttttcccaaaatcctcaagtacgctatttaaatcccctgtggatatcagtcagcagtc
ttcaacttagcaacaggtatcacatcatacaggcccatttgctgaagctccgtctaccacaatttgttcagaggttctctgttcttgactgctaccactg
ggctgtgaaggaggaggattttccattgctgtgtcaaagttttccactacaccatatttacaagctgaatcatgagaggtagtcagtttcccttcctcac
ttttaaaaacagcggtttctgtttttgcatctacggtaaggctgagcgtttccttcatgctgccattcatcacagtttctgtcatctgatctgcattttc
cacctcctcttccccatcagaaactgcatccatagcaacagtaacttctgtttgtgctgatgcatttacactgagaggatccactggctctgtgcttgtt
tccatttcaacaggggagctacttcttactgtctcctttgtactaatttgagatgtgaattc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: