XB-CLONE-3580127
Clone Name: IMAGE:7693946
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Gene:
tbcel
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC152485 [+]
AGGAGGGAAAAATAGAATGTTTGGTGGCAGTTTCTCCTGTGTCAGGACAGGGAATAAATAGTTACACTACACACAGTAACACAAGTTTTACTGTTAATAA
TGTGGTCCTGGACGGAGGTCCTGTCATGGAATGTGGAAACAATGGATCCATCCTGTGGAAGGAGCTTTATGCAGGTCCTTTGTGAGAAGTACAGCCCAGA
AAACTTCCCATATAGAAGGGGTCCTGGAGCCGGGGTTCATGTTCCCCCTGGACCGCAGGTGTCTCCTATGAAAGACCGGCTCAATCTCCCTAGTATCCTG
GTTCTGAACAGCTGTGGGATCAACTGTGCTGGAGATGAAAGTGAGATTGCTGCTTTTTGCTCCCATGTATCAGAGCTGGATCTCTCTGATAATAAGCTTG
ATGACTGGGAGGAGGTTAGCAAGATTGTGTCCAGTGTTCCCCAGTTACAATTTCTTAATCTGAGTTCCAACCCACTGCACCTGTCAGTTTTGGGACGTTC
ATGCGCCGGGACATTCTCTAGCATTCGCAAACTGGTCCTCAATAACTGCAAAGCCTCTTGGGAAACGGTCCATACACTGTTGATGGAGCTGCCAGAGTTG
GAGGAGCTCTTTCTGTGCCTTAATGACTATAACACAGTATTATGTTCACCAGTCTGCTTCCGCTCTCTGAAGCTTCTCCACATAACAGACAACAACCTCC
AGGACTGGTCCGATATCCGCAAGTTAGGCACAATGTTTCCATGTCTTGACACACTTGTTCTTGCAAATAACAACCTGTCTAAGATTGAGGACTCAGAGGA
ATCTTTAGCAAGGCTGTTTCCCAATCTAAGGTCAATAAGTCTGCATAAAGCAGGTTTAAATTCCTGGCGTGACATTGAAAAACTTAATGCTTTACCCAAA
CTTGAAGAGGTTAGATTATTAGGTATTCCTCTGGTGGAGCCATACAGCACAGAAGAACGCAGGAAACTTGTGATAGCAAGATTGCCGTCTGTATCCAAAC
TGAATGGCAGTGTTGTCACGGATGGTGAAAGAGAAGACTCTGAAAGATTCTTCCTGCGTTACTATATGAATCTCCCAAAGGAAGATGTCCCATTAAGATA
TCAGGAGCTGGTGACAAAGTATGGCAAATTAGAACCGCTTGTAGAAGTGGATCTTCGACCACAGAGTAAAGCAAACGTTGATATACACTTTGAAGAGAAA
GTGGAGACAATAAGCATCCGTTTAGATCAGACTGTGGCCGAGTTCAAAAAACAGCTAAAAAATTTGGTGCAGCTTTCTACCAACAACATGCGCCTGTACT
ATCTGGACCATGAAGCCCCTTTTGGGCCAGAGGAAATGAAATTCAGTACCCGCTTTTTACATTCATATAATGTTCATGATGGCGACAAATTCTATGTGGA
ACCCAAAATCAAATGACCGTACATGCCTCTTCCTATAGTTATATATACACTCACACTGTATATATATATATATATATATATATATATATATATATATATA
TATATATATATATATATATATATATATATATATATATATATATATATATATATATATGGACATTACAAGGGATTTTTGGTTTTGTTATAATTGCTTTTAT
ATAAATTAACCTCTTTGCCCCCCTGGTGCCAACCGCCAGCTCTAGCACATTGAAGAGCTATGTGCAGGTGCACAAGTGGACAATAAAGGGGTCTTCATCA
GCTTAGGGACCTCTACATCATAGCAGGCTCTTTAAGGAGAGCCCTACCTGGAGCACTTTGTGTTTGTGAGTTATTTACAAAACTGAAAATATTGCAGTTA
TTTTTTTTTCTATTTAGTTGAGTCAAGGTATATTACCCTTTACACATAACTGTGTATATAAATATATATAATAATTTATTGCCATGATTACCTTAGTTCT
TTCATGTCTCTGATTATGCAGATTACTTTTTAGAGTTTTTCTATTTTATGCTTCTCTTCAATTTGATGCCCTTTACTTTCATGAGATCTACTGGTGTCCA
GCATGTGGTAATCACCCTGTTTTCCCCCATTGTCTGTGTTTATTTATTATACCAGTGACATAGTGGGGAATATTGGTATTGGCACAGACAATCAGATATC
AAGCTATTGGAGCTGACAAAGAGCCAATGAAAAATTCAAGTCACAGTACATATTCTTGTTTGTTTGTTTTTTATCTTTTTTTTACCTACTTAACTTCCTG
GCCATACCCTAAATTACTCCATGTTTGTAAACCTTTGCTAATGTATCATTTTTATACATGTCATTTTTCACTATTTATGTTAGGCTACATATTTTTGAGA
CAAGGTACACCCTTTTAATGTCCAAGGGATTTTACAGAACAAAGTTCACATTTATGATGGCCGTGCAAGTGCCAAAACAGGCTCAATGCACCATTGCCAT
TTACTTCTTGCCCCTTTTTGGGATTGCAAAATAAAGTACAAAGAGTTGCTCCCATTTCTGTATGCTGTATGCAGTGGATGGTATAAGTGAAAGTACTTAA
AACATTCATTACAGGATGCCCTTGCACCCTGTGCATTCTGTGCTCTTTGCCTTGCAACAGATATTTATTGTGGCTCAGGAGGCAGTGCGCTAGAGACCTC
TAAGCGGTACAGTTTTGCTGGAGTGCTTATGACTATGGCATAGCGTAAATGAGTCTACGTGTTACCTGCTTTTAGTTTATTGTTTTTAATTTGTGATTTT
TAAAAATCTGTCTCAGCAAATAGGCTGGCAGTAAAGAAAGCCAGTAAAAATGGTATGGCTTCCCAAGTCACTGTGACCAGGCAGTCCAAACTATGCGATA
CACAATGAATGGGATTACTTGTGATTTTTTCTTTTAATCTGTTCGTTTAGGGCAAAAAATTGTTGTAAAGCATCTGTGTGTAGAAAAAGGCATACTAGCT
GACTTCCCCTGCCACAATAAGTTTTTTCTAAGAATGTTTGCCAGAAATACATTTTGCAGTCTGTGGAGTTTATTTATCCAAGTGCCTAAAATACTAATGA
ATACACCCAAAACAGAATACTATTGAAAAGTGTATTATTTGGTAATGAAGTGTTACATTTGTGTTATATCCTAAGTATTAGCGGTGCTTCTTATCCTTCT
ACTCCCTTTGTTTTGCTCCTTTTTATATGTATATTTCATTTATGCACAACTGTTGTTTGGAAGGTTTTGTATATCCCTCCCAATAGATGATGCTCCATAA
ACATTGAAAGATCACTATTTCAATATTGCATGTAAAATATTTACTTTCTTTCCTGGTGTCCATCTTTGTTCAATATACATCTTTTCCAGTCTCCATCCTA
TGCTTTCTCTGTGACAGTGTTTGGAAGATTCTGTTTCCTTCCTGGTTAAGCAAATCTGAAACAGCAACAATGCTAATTGTGCAGAGTTTTCCCAAGGGGT
ATTGCTCTAACTCACATTTCACTCCAAGGGGTCAGACAAACAAGGACTCTTTAACTGGCAGAGACCTAGAAGAGTGCAACAAGCAGAATGGAACATAACA
TAGTTCCTTGAACAATGTTAACAATCCATGTTCTTATACAGATCAGAGCAAAACTATTCCCATATGGCAGTGTTCTGATGCTTATGGGGGTAAAGAGATA
CAGAAAAGCAATTTGATCTACGTTAATGCTGGCAACTGTAATTTTACAGACTCTGCATTATAAGGGTATGTAGTGTCCATTGTTTTGCACCAAAGTTGGT
TAGGTACTAATTTATCCAAGGGGTTTGTTTAAAGGCTGCATCTAATAAACCATAGGCAATGCTGCAGGCATTTCTCCATTCTAGCAGTAAATCCACCCTA
AGGCAGTTGCAATTTATGTGGTCCTGAACAAAGATTTTACCTTCTAATCATTGTTTTATTAATATCCAGCAGTAAACAAGTGAACCTAAAAGCTAACTGA
TATTATCATTCTTTTGAGTTTAAACTCTAGGCCTGGTGAACCTAGCCTGGAACTGCTAGTTGTGATGTGGCTGCCTTGTTAGTGGTTATTGTATTTGAAC
ACAACATCCTTTATGAATCCTGCATTGAAGTTTGTAGAATTGTGTTTTTTTTACTCAGCATTTTTCCAGGCTACATGTGACCATTTTAGATTGCAAATTG
GTACCATTTTTTAGGAGAACTTAGAGGGCTTAAGGGAAAAAGCACAAAATATGGGGGGGGGGGGGGTCAATGTTTTAAATAGGTGTTTAAACATAAAGCA
TGATTTGGCCATTATGCTAAGTAACTTAAAGGAACTCATTTTTATAATGCAAATGTTCTCTTGTCTGTGAAATCATCATTTCAGTTTATTTTTCTAATGT
TCTGAATGCAACACTTCCTGTGGATTATGATGCTGAATGAGGATCTAGACCTCTGCACCCCACCAAACTGCAAGCCAGTGCACCATCTATGTGGCTAATG
ACTGCCAAAGACTTCACAGATTCAGTAGGATGGTTTGGCCAGCTTTCCATCAGTTCTTCATTTAGAGTAAAGCAAACACTACTCCCATTTGCAGTGGAGC
AGCTAGACTGTTGTTCATGCTTGTAATTGAGCTTTTAGTATATATTGTAATATTACTGATCTCTTGGTGCATTCACCAGGTCTTCAGTGATGCCCTATAT
AGCCTCTTTACTACCTTTATATTGAGTCCAGATTGGAATGCATTTCTGATTAAAAAAAAAAACCCAATGTGAATAATTGCTAAATAATAAAGTTTAGTTT
TGGATAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX921695 [+]aggagggaaaaatagaatgtttggtggcagtttctcctgtgtcaggacagggaataaatagttacactacacacagtaacacaagttttactgttaataa
tgtggtcctggacggaggtcctgtcatggaatgtggaaacaatggatccatcctgtggaaggagctttatgcaggtcctttgtgagaagtacagcccaga
aaacttcccatatagaaggggtcctggagccggggttcatgttccccctggaccgcaggtgtctcctatgaaagaccggctcaatctccctagtatcctg
gttctgaacagctgtgggatcaactgtgctggagatgaaagtgagattgctgctttttgctcccatgtatcagagctggatctctctgataataagcttg
atgactgggaggaggttagcaagattgtgtccagtgttccccagttacaatttcttaatctgagttccaacccactgcacctgtcagttttgggacgttc
atgcgccgggacattctctagcattcgcaaactggtcctcaataactgcaaagcctcttgggaaacggtccatacactgttgatggagctgccagagttg
gaggagctctttctgtgccttaatgactataacacagtattatgttcaccagtctgcttccgctctctgaagcttctccacataacagacaacaacctcc
aggactggtccgatatccgcaagtta
3' EST: CX921694 [+]aactatccaaaactaaactttattatttagcaattattcccattgggtttttttttttaatcagaaatgcattccaatctggactcaatataaagggagt
aaagaggctatatagggcatccctgaagacctggtgaatgcccccagagatcagtaatattacaatatatactaaaagctcaattacaagcatgaacaac
agtctagctgctccactgcaaatgggagtagggtttgctttaccccaaatgaaaaactgatggaaagctggccaaaccatcctactgaatctgtgaagtc
tttggcagtcattagccacatagatggtgcactggcttgcagtttgggggggtgcagaggtctagatcctcattcagcatcataatccacaggaagggtt
gctttcagaacattagaaaaataaactgaaatgatgatttcacagacaagagaacatttgcattataaaaatgagttcctttaagttacttagcataatg
gccaaatcatgctttatgtttaaacacctatttaaaacattgacccccccccccccccaattatgggctttt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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