XB-CLONE-3580096
Clone Name: IMAGE:7694237
|
Gene:
polr2b
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX921723 [+]
tttgtgtctgacagggacatattagaacatattatttatgactttgaagatccagagatgatggaaatggtcaaaccatcgcttgacgaagcttttgtta
ttcaggaacaaaacgtggctcttaattttattggttctagaggtgctaagcctggtgttacaaaagaaaaaaggatcaaatatgcaaaagaagtcctaca
gaaggaaatgcttccacatgttggtgtcagtgatttctgtgaaacaaaaaaagcttactttctaggatacatggttcacaggctacttctggcggctttg
ggaagaagagaacttgatgacagagatcattatggtaacaaaagattggatctggctgggccattactggcttttctttttcgagggatgttcaagaatt
tgctaaaagaagttcgaatttatgcacaaaaatttattgatcgaggaaaagattttaatctggaactagcaatcaaaaccaggataatttctgatggatt
gaagtattctttagccactggaaattggggtgaccaaaagaaagcacatcaagcaagagctggtgtgtcccaggtgttaaatcggctaacctttgcatca
actctttctcatctccggagattaaattcccctattgggagggatgggaaacttgcaaaaccacgacaattgcacaacactctgtggggaatggtttgtc
ctgctgagaccccagagggccatgctgtaggacttgttaagaatttggcgttgatggcctacatttccgttggttctcagccgtcacctatactagagtt
ctt
3' EST: CX921722 [+]caaatttcacactttattcacccctgtaaaaatacacttacaatacataggcacagtttaagcaaagagtaatgcattatttgcaaaacaatttattata
ttttacattaaaaggcaacctaatatcacattatgatgtaataaacataaaaaatgtattatgaggtcatcatgcgtggagcaatactcattgacattaa
ttcttgaaacagaagtttgcaggcatagggcattcgcaccaaagatatctgtgttttgttccgacatcccctacattcatatgtatgggttcttgtattg
gcaattgccatcaagccacagagattgcagacatgaacttgataaggatcagaagcttcaaacaacctctctcttaagaactgagcagctccatgggcaa
tctgacaatcacgttccatttctccaaaacgtagacctccatcccgggatctgccttccatgggttgtctattcaaaatctgaataggacctctagcacg
ggaatgaattttatcatccaccatgtgctttaatctctggtaataggttggcccaatgaagatctgggatgtaattttacgtccagtgaatccattgtac
agaacctcatttcctcgaagatgatatccataatccgataacagatttgaaattttttgcacatttacagcatcattaaagggtgttgcatcacctattt
cccctttgtttgcagataccttcccctgcagacattcaatcaaatgtccaactgtcatacgagaagggattgcatgagggttgatgataatatcaggtgt
aattcct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: