XB-CLONE-3578872
Clone Name: IMAGE:7694598
|
Gene:
mtfr2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC121899 [+]
CCAATGTACCCTGCAAGTTCTGCTTCCTGCTGCTGGCTTAGCTGCGCTCGGCACTGGGAAGTCAGAGGTGCACGCTTTCTGCGGGAACACGGGCATTTAT
TGATGTAAAGCACCTCTCTGCAGTGGTAGTGCCTTGCTTCATGCACACATGCCAAACTGGTAATGAGGAAGGTAACTGAGAGGATTCTGAGAAGGTGAAT
TAACTCCTACAAGTCTTGGAGAAAATTTGGGTGCTCAATGTGGTGAATTATATCCAAGGTGTATCAATGTCTTTACTGTTGGAGCTCCTGCGGGAACTGA
TGGAATATCTAGGTATTCCAGTTGATAGGCTTGTTCCAATATGGGAAAACTATGCCAACAGGCAATATTTTCTCCGTGCTCTAATGAACCATCTCCCTTC
AGTTTTACCAGGAATTAATTTGCAGGTTATAGAAAGATTGGAGTGTAAAGAATATGGCTGGACTCGCAGTGCGGTTCGGGTGATTGGAACTTTACTGCCA
TTGGCTCCTCCCCCAAGACCCTTCTTCCAGCATGTCACAGGGCCTTATGCAGTAGAAGGAAAGAGCGATGGAGCAGTATGCAGGCCTGGGATTCCATCAC
TGGGTGATGTTAATTGTTTGACAGACGATGATGTTTACTCTTACACCAGATTTAGGCATGATCTACCAGGAAACCGAAGTAGAGAAATAAAGTCTATAGC
ACCATCTTTCAAGAGAAATCTGAGTTGGGAGCCCATGATATTAAATAGAGTGCCAGATAAAAGTGTCCCCTCAAGTGAAAATGCTATTCAGAAAATTGCC
GCCCTAGAAGATGAACTGTTGCAGCTAAGGGCACAAATCGCTGCAATTGTTGCTGTACAAGAAAACAAATCCATGCAGTCCTGTTCTGAAACCCTTTCTT
CATTTGGCAGTCCCACTCCTGATTATCCCCAGCCATCTTTAACTTCTACTCCACTTTCGGGTCAGATACTGCAGCCTGTAACCCCAGCACCCCCGCCACC
TCCACCACCACCTCCTCCACCTCCTCCTGCAAAAGTAAATTCTGGCGAATCTGCAATAGAACTTATAAAAAAACGCAAGGGAGTTCACAAGCGAAGCCCA
AGAGAGAGGGCAATGGTAGATAATACAAATAACCCGCCCAGTATGATGGATGTACTGAAAGGCATGAATTCTGTTAGACTGCGTGCTGTAGAGAGGTCCC
CTGGCGGAACACCCCTAACAAGAAAAGACAAAAAGAGGCGTTCCTTAGACGATCCAGCAGCTATTATTGCCAATGCCTTAAAGCAGAAATTTGCTCATAG
ACTCAAAAATGACTCATTTGATAAGGAGAACATTTCACAGGAAGAATCTCCTTTCTCCAGTCCTGAAATTCCACAGTTTGGGCGGCACCTTTTAAAGCCC
ATTGGAAAGCGACCACAGGCACTGCAAGACGGAAAACAAGTTTTGGTGAAACCCAGACCCCATGTATACCATTAAATGTAGCGCAATTCTGAGCTACTAC
AAAGACTTTTATCATAAAAGAGTTCAATGCTGTTGGTAATTTATGATTATTTCAACAAACAAAAAAGAATGTAAGTTTTATTTTTAAAAATATCTGCCTT
ATTTTGTCTGTCACCTATGTCATACACTGGTGGGTATTTAATAGGTTGCAAAGTAACAAAGTAGGCAGCAAGTCTAAAATGACACAAGTTGACTGGTTTT
AACTTGGCTTACCTTAATTCTTTTAAATTCTTTTTGTTTCTTAAATAGGATGGTAGAGTATAAGCGTGCAGCTTGTGTGCATATTTGGTTTTATGCTTGT
AGATAGGAGCTGGCATATTTCTTGCTTCAGTACCTATAGTAGCCAAAAACATGGTGCATTTGCTCTTGCATTCTGTGGATGGCACTAAATTAGCTTTGCT
TTTGTACAATACTAAATTAACTTTGCTTTTGTACAATACTAGCCATTAAGGACAACAAAAAGAAGATTAACACATCTTGTTAATAGGGTATATTTACATC
TGGCTTCAATGATAGACAAGTAGAAGCCATGTAGAAATCTGCTGCTGCTTTTATGTGTAATGACAGTTGAAAGAATTATTTTGATGACTTGTACTGTTAA
AGGTTCAGCCTCTCCTTTAGTTATTTATTGATGCTGGTTTATTTTTTACTTCTGTATTTTATTCCATAGTCTCCTTGTTTATGCAGATGATCTTTTTATG
AGCAGTTTGTATCTGTGTTGTTTTCCCCTAACCTTTACTGTGCATGGCAAGGCAGAGGGCTCTGCCATTTGCCTCACTCTGATAAGTGTGGCAGAGTTAA
GTCATCCAAGTGGGCTCATCAAGACCTAGGCTGTTTGTTGTTGGGGGGGCACTGAGTTTTAGAAACTTTTGGGAGTCAAGCACACAACTGGACATGTTTA
TGTGACAACTAGGCAACATTCGGCAGAGGATCTCTCCTTGCATTCTTTTAAAAGGGATGCATCCTAGGACAGCAATTGCATATTAAAGGTTCGTCCTTTT
GAACAAAAGTAGAAATATGTGCAGAAAAGTCATAGGAGAGATTGTTTTTAATGATAAAAACTATTCTAAATGTGTGGTGATTTGAAATGTCTATGGTAAG
TTTTAACACTTGTTTATCTACTGTATATGTTTGATTATTTGATAATGATTATTTGTTCTTTTTAATTAAGACCAGTAACTGGTAAGCTTTGAAGCAGGGC
TGTCAAACTGGAGGCCAGATTTATGCTTCCCAGACTGCTCAGGAACTCAATGACATCTATAGGTGACATCATTGTTTTCTTTTATTATGGCCCCAAAACA
TATAGTACCTGTATATTAAATAACTGCCTTTAAGAGCAAAAATGTATGATACTTGAGTGATGTTATTCTCTCAAGACGCAACACTGTTCTTTTTCAGCAG
CCTTTTTAAGTTAAATGGGAAATGCAGCAAATACAGAAGGGCACTTCTGTCTGCAGACAGGTATGCCCTAGAAGACACATTTTACCATTTGCCTAGCTTC
TGTGGTACATTTCTCTCTCACATGAATGCAATATGGCTATATTTATCATCTTGCATTTTATGGCTCTTTTTGAGAGGACATAAGCAATTACAATATCTGA
TGTAACATTGCCATGGCCCCCTAATAGGCTTTACTTCTATATCAGTCAATTGTTGGCCTTTACAAATTGAAAGCAGGTTCTTTCACTTTTAATTGTAAGA
GTAAGGGTGCATATTTATTTTGTTAGGAGTAAAGTCAGGCAGCATCATGCCTTTCCAGTCTGGTCAGCTCAGTATGCCCATTTGTTTGCCTCAAAATAAG
GTGACTTTTGGTAGTTTTGCTTCAGTTGAATTCACAATGTTAGTATTTGGCAGCTCAGAGGTACAAATTGTAACCCTATGAGAGCCTGCACTGTGCAGCC
ACTGGCTGTTTTGACTTGCCGGTAGTATTTCAATCTCAGCAGCAATTTATTTCCTGATGTTTTCTTAACTCCTTCCAAGCAATATCATAAATATTATTTG
AACCATTATATGTTCGGTTTCCACAACCAATCCATTACAAAGTTTAAAAACAACGCTTTACATGTATTTTTCAGTTTGTGCTGTTACATGCTGTCAAACA
TTTTCTGTGTTTATATCTTAAAGACAACACCATTTTTGTGTTTACATTTTTAGGGCTCTGGTATGGTTACAGTTCAAAAATACAAATGTGACCTTTATGT
TTGTACACCAATATATATATGCTTTAATTGCACTCGACAACTTCTGTACAAATTATAGTTTTATTTCTTTTTGTAAATACGGTTTTAAAATAAATCTTTA
GATATTCTAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX922976 [+]ccaatgtaccctgcaagttctgcttcctgctgctggcttagctgcgctcggcactgggaagtcagaggtgcacgctttctgcgggaacacgggcatttat
tgatgtaaagcacctctctgcagtggtagtgccttgcttcatgcacacatgccaaactggtaatgaggaaggtaactgagaggattctgagaaggtgaat
taactcctacaagtcttggagaaaatttgggtgctcaatgtggtgaattatatccaaggtgtatcaatgtctttactgttggagctcctgcgggaactga
tggaatatctaggtattccagttgataggcttgttccaatatgggaaaactatgccaacaggcaatattttctccgtgctctaatgaaccatctcccttc
agttttaccaggaattaatttgcaggttatagaaagattggagtgtaaagaatatggctggactcgcagtgcggttcgggtgattggaactttactgcca
ttggctcctcccccaagacccttcttccagcatgtcacagggccttatgcagtagaaggaaagagcgatggagcagtatgcaggcctgggattccatcac
tgggtgatgttaattgtttgacagacgatgatgtttactcttacaccagatttaggcatgatctaccaggaaaccgaagtagagaaataaagtctatagc
accatctttcaagagaaatctgagttgggagcccatgatattaaatagagtgccagataaaagtgtcccctcaagtgaaaatgctattcagaaaattgcc
gccct
3' EST: CX922975 [+]agaatatctaaagatttattttaaaaccgtatttacaaaaagaaataaaactataatttgtacagaagttgtcgagtgcaattaaagcatatatatattg
gtgtacaaacataaaggtcacatttgtatttttgaactgtaaccataccagagccctaaaaatgtaaacacaaaaatggtgttgtctttaagatataaac
acagaaaatgtttgacagcatgtaacagcacaaactgaaaaatacatgtaaagcgttgtttttaaactttgtaatggattggttgtggaaaccgaacata
taatggttcaaataatatttatgatattgcttggaaggagttaagaaaacatcaggaaataaattgctgctgagattgaaatactaccggcaagtcaaaa
cagccagtggctgcacagtgcaggctctcatagggttacaatttgtacctctgagctgccaaatactaacattgtgaattcaactgaagcaaaactacca
aaagtcaccttattttgaggcaaacaaatgggcatactgagctgaccagactggaaaggcatgatgctgcctgactttactcctaacaaaataaatatgc
acccttactcttacaattaaaagtgaaagaacctgctttcaatttgtaaaggccaacaattgactgatatagaagtaaagcctattagggggccatggca
atgttacatcagatattgtaattgcttatgtcctctcaaaaagagccataaaatgcaagatgataaatatagccatattgcattcatgtg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: