XB-CLONE-3578208
Clone Name: IMAGE:7694930
|
Gene:
agtpbp1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC155703 [+]
CTGGGAGCCTGCAGTTGACAGCTTTGCAGGAGGGGAAGTGAAATGATAAAATTAATGACTTCTTCAAGATGAGTAAAGCAAAAGCAACAGCAGACAAATG
CCTGTCAAACAATTCCCGGATTCAGAGTCTTTTATCCCAGCTGGAAAAGGTCAATGCAGAACCTCTATTATTTGACTCCGATAACACAAGATATGTTACT
GCAAAGATTCTTCACTTGGCACAAACTCAAGAAAAAACCCGAAAAGAAATCGCAGCCAAAGGTTCAACTGGTATGGAAGTGATACTTTGTACGCTTGAGA
ACACCAGGGATCTACAAACTATACTGAATATTTTAAATATTCTGAATGAACTGGCATCAACTGCTGGTGGCCGTCGAATCAATGCCTTGATATCAAAGGG
TGGCTCCCGGATACTGTTACAACTTCTTCTAAGTGCCAGCAAGGAGTCCTCTTCAAATGAAGAGCTAATGATGGTTCTTCATAGCTTACTTGCTAAGGTT
GGACCAAAAGATAAGAAGTTTGGAATAAAAGCACGTGTTAGTGGAGCATTAAACATATCCTTGAATTTGGTCAAACAGAATTTGCAAAATCCAAGGCTAA
TCCTTCCCTGTCTGCAAGTGCTACGTGTCTGTTGTATGAATTCTGTGAACTCTGTATACCTGGGGAAAAATGGAGCTGTGGAAATCCTCTTTAAGTTAAT
TGGACCATTTACAAGGAGGAACACAGGCCTCATAAAGGTATCTTTAGATACTTTTGCTGCTTTGTTAAAATCAAAAACAAATGCCAGGAAAGCTGTTGAC
CGGGGATATGTCCAGGCACTGTTATCCATATATACAGACTGGCATCGCCATGACACCAGGCACAGACACATGCTGATCAGGAAAGGAATATTACAGTGCC
TTAAAAGCATTACAAATATCAAAATAGGTAGAAAAGCTTTTATTGATGCTAACGGCATGAAAACTCTGTACAACACATCCCAGGAATGCCAGGCTGTTAG
AACATTAGATCCACTTGTCAACACTTCAAGCTTAATTATGAGAAAATGTTTTCCAAAAAACCGTCTTCCATTGCCTACAATCAAAAGTGCTTTCCAGTTT
CAACTCCCCATCATGCCTACAAGTGGACCTGTTGCTCTTCTTTACAGTATGCCCCCAGAAGTGGATGATGTGGTAGATGAGAGTGATGACAATGACGACA
CTGAAGCAGAGACAGAAGCTGAGGCAGAAAATGAAGAAAGTGACCAGCTCTGTAAGAATGATGATATAGAAACAGATATTACTAAGCTTATACCTGGACA
AGAATTGGGACGGACGTTGGAGGATTTAAAAATGTACGAGCGCTTTTTCCCAGAGTTGACAGAAGATTTCCAGGAATTTGATCTGGTTTCAAATGAACCA
AAACCTGGAGCAAAGCTTGGGCCTATTATTGTACCGACTGCTGGAGAGGAACAGCCAGAGGTGCCAAATAACTTCATGAAGGATTTGGAGAAAAGAAGTT
GTAATATCTCCTTGGAAGATGAATGTAATAAAAGACCAACATTTCTGGATATGCCAAAAAATGTAACGAACAAAGGGAACGACGGTCTAGGTCAACAGGT
TCATGGGGACATTGACAGATCTTGTTACTACTTCAGTAGTGATATTGTAAAAGATCTTGAAAAACTTTCACTGCGCAAACCTTCAGGAAACCACCCTTGC
AGAAATGGATGTGTTTCAGCTAAAGATAAGCCTATTTTTTTGCCTCACCCATGTAATAAGTCAACATCTAATTCTGGTTCCTGTAGTAACAACTTGTTTG
AGAAACATTCTATGCACCTCAGCCCATTGTGTTGCTCTGGAATTACACCTGATGATGATGAATCAAGTCCACTGGATGAACTAGCCATGAGGGAAATAAC
AGACTTTGACAACTTACTACCTCTTCATGACCCTGACCTTTATATTGAGATTGTGAAAAATACTAAATCAGTTCCAGAATACTCAGAAGTGGCATATCCA
GACTATTTTGGCCATGTGCCTCCATTTTTTAAAGAGCGCCTTTTGGAAAGACCATATGGTGTTCAAAGGTCAAAAATATTCCAGGATATAGAAAGGATGA
TTCATCCAAATGATATTATAGACAGGGTGGTATATGACCTGGATAATTCAAGTTGCTCTGCACAGGATGAGAGTGAAGTTCTTAAGTTTAACTCCAAATT
TGAATCTGGAAACTTACGGAAAGTCATTCAAATAAGAAAAAATGAATATGATCTTATCCTGAATTCGGATATAAACAGCAACCATTACCATCAGTGGTTT
TACTTTGAAGTAAGTGGCATGAGGACAGGCGTTGCTTATCGATTCAACATAATCAATTGTGAGAAGTCCAATAGCCAATTTAACTATGGTATGCAGCCTC
TAATGTACTCTGTGCAGGAAGCATTAGCATCCAGGCCTTGGTGGTATCGTGTTGGCATGGATATCTGTTATTACAAGAATCACTTTTCCAGAAGTTCAAT
AGCCACTGGAGGTCAAAAGGGAAAATCATATTATACTATCACATTTACAGTAACATTTCCACACAGAGACGACGTCTGTTATTTTGCCTACCATTACCCC
TATACTTATTCCACATTAAAGATGCACCTTAAAAAACTGGAGTCTTTGCACAACCCCCAGCAAGTCTATTTCCGCCAAGAAGTATTATGTGAAACACTAG
GTGGAAATGGATGTCCTGTAATCACCATAACTGCTATGCCAGAGTCTAACTATTATGAGCATGTTTATCAGTTCCGTAACCGCCCTTATATTTTCCTTTC
ATCCCGTGTTCATCCTGGAGAGACTAATGCAAGTTGGGTTATGAAAGGCACTTTAGAATTCTTAATGGGTAGTAGTACCAGTGCACAGAGCCTGCGCGAG
TCTTATATTTTCAAAATAGTTCCTATGCTAAATCCGGATGGTGTGATCAATGGAAACCACCGTTGTTCATTAAGTGGGGAAGATCTAAACAGACAGTGGC
AAAATCCGAATGTCGATCTGCATCCTACAATATACCATACCAAGGGACTGTTACAGTACTTGGCAGCTATAAGACGCACACCTTTGGTTTATTGTGATTA
CCATGGCCATTCTCGCAAGAAAAATGTGTTTATGTATGGTTGCAGCATTAAAGAAACGGTATGGCACACTAATGCTAATGCTGCCTCATGTGATATGGTG
GAAGATAGTGGATACAGGACACTTCCCAAGGTTTTAAACCAGCTAGCTCCAGCATTTTCAATGAGCAGCTGTAGTTTTGTGGTGGAAAAATCCAAGGAGT
CGACTGCCCGAGTTGTCGTCTGGAGAGAAATTGGAGTCCAGCGAAGTTACACAATGGAAAGCACGTTGTGTGGTTGTGATCAAGGAAAGTATAAGGATTT
GCAAATTGGAACTAAGGAATTGGAAGAGATGGGTGCCAAGTTCTGTGTAGGACTACTTCGTTTAAAGCGACTAACCTCACCTATGGAACTCACTCTGCCT
CCAAGCCTTATTGACATAGAAAATGAACTGATTGAGTCGAGTTGCAAAGTAGCAAGTCCCACCACTTATGTCTTAGAGGATGACGAACCTCGGTTCCTCG
AGGAAGTTGACTACAGTGCAGAAAGCAATGATGATGCGGATCCTGACTTACCAGATACTATTGGTGATTTTGAAAACACTGCATTGGAGGAAGAAGGATT
TTCGGATTCGGAGATCACAAGGACTCACACAAGCGGACAATCCACATAACCTTTAAAGGATATATATATTTTAGATTTGAAAGCATCATTTAAAGCATCT
TTGCTGCTGTATTAAATATTAAATAAGCTGCACCCACTCTATACACCGTGCAGGCTGGAAGCTTACCCCATTTAATGTGACAACCTATTTTTACAATAAA
TGTGGTAAGCTCACTGCTTGCATGTTATATAAAGTGTTTCTGGCTCCTTCAATACTGTATGTTACAGTCTATGACTTATGCTCTTCATCTAGGGGGTCTG
AACCACCATTGCAGGAGTGTGTGATGTGTTGGTTGTGCAGCTGCGTATACTTGTTGGACATTGGCTTCTTTTCTGCCTTCATAAGGAAAATACCAAAAGC
TTATTTAAGTACTGTAAATCAATAAATGCGTAGCAGGCTTTTGATGGCTTTAAAAAAAAAAAAAAAAA
5' EST: CX923670 [+]ctgggagcctgcagttgacagctttgcaggaggggaagtgaaatgataaaattaatgacttcttcaagatgagtaaagcaaaagcaacagcagacaaatg
cctgtcaaacaattcccggattcagagtcttttatcccagctggaaaaggtcaatgcagaacctctattatttgactccgataacacaagatatgttact
gcaaagattcttcacttggcacaaactcaagaaaaaacccgaaaagaaatcgcagccaaaggttcaactggtatggaagtgatactttgtacgcttgaga
acaccagggatctacaaactatactgaatattttaaatattctgaatgaactggcatcaactgctggtggccgtcgaatcaatgccttgatatcaaaggg
tggctcccggatactgttacaacttcttctaagtgccagcaaggagtcctcttcaaatgaagagctaatgatggttcttcatagcttacttgctaaggtt
ggaccaaaagataagaagtttggaataaaagcacgtgttagtggagcattaaacatatccttgaatttggtcaaacagaatttgcaaaatccaaggctaa
tccttccctgtctgcaagtgctacgtgtctgttgtatgaattctgtgaactctgtatacctggggaaaaatggagctgtggaaatcctctttaagttaat
tggaccatttacaaggaggaacacaggcctcataaaggtatctttagatacttttgctgcttgttaaaatcaaaaacaaatgccaggaaagctgttgacc
ggggatatgtccaggcactg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: