XB-CLONE-3577414
Clone Name: IMAGE:7695432
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Gene:
apc
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121432 [+]
GCGATCAGGAGGCAGGATGGAGAGCTCCGGAGCCATGGTGCTGCTGCTGATGGGGCCTGGGGGTAGCCCAGCATGGCTGCTGCTTCGTATGACCAGTTGT
TAAAGCAAGTGGCGGCACTGAAGATGGAGAATACAAACCTTCGACAAGAATTAGAAGACAATTCCAATCATCTTACAAAACTAGAGAGTGAGGCATCAAA
CATGAAGGAGGTTCTCAAGCAGCTACAAGGAAGTATCGAGGATGAGGCAATGGCTTCTTCTGGCCAGATTGATCTGCTGGAGCGTCTTAAAGAACTAAAT
TTAGACAGCAGTAACTTCCCTGCAGGCAAGACTAGGCCAAAGATGTCTATGCGTTCTTACGGAAGCAGGGAAGGCTCTCTCTCTGGTCATTCGGGTGAAT
GCAGTCCGGTCCCTGTGGGTTCCTTTCAAAGGAGAGGCCTTTTAAATGGAAGCAGAGAAAGTACTGGGTATCTGGAAGAACTTGAGAAGGAAAGGTTATT
ACTTATTGCTGAGCATGAAAAGGAAGAAAAAGAAAAGCGCTGGTACTATGCCCAACTTCAGAACCTTACCAAGAGGATCGATAGTCTCCCACTTACAGAA
AATTTTTCTCTGCAGACTGATATGACCAGAAGGCAGCTAGAGTATGAGGCACGGCAGATCAGAGCTGCAATGGAGGAGCAGTTGGGAACATGTCAGGACA
TGGAGAAGCGAGTACAGACAAGAGTGGGCAAAATCCAGCAAATAGAGGAAGATATATTACGCATTCGGCAGCTATTGCAGTCCCAAGCAGCAGAGGCAGC
AGAGCGGACTCCACAGAGCAAGCATGATGCAGGCAGCAGGGATGCCGACAAGCTGCCCGATGGACAGGGGACCTCAGAAATCGCTACATCCAGCAATGTT
GGAAGTGGACAGGGTTCATCAAGTAGAGCAGATCACGACACAGCCAGTGTTATGAGCTCTAACAGCACTTACTCTGTTCCGCGAAGGCTCACTAGTCATC
TTGGTACCAAGGTGGAAATGGTATATTCACTTCTGTCAATGCTTGGTACTCATGACAAGGATGACATGTCTCGGACTTTATTAGCCATGTCAAGTTCTCA
AGATAGCTGCATTGCTATGCGCCAGTCTGGGTGTCTTCCTCTTCTCATTCAGCTATTGCATGGCAACGATAAAGACTCTGTGCTGCTGGGAAACTCCCGT
GGAAGCAAAGAGGCCCGTGCTAGGGCTAGTGCAGCTCTACATAACATCATTCACTCTCAGCCTGACGACAAGCGAGGGAGAAGGGAAATCCGTGTCCTTC
ACCTCTTGGAGCAGATCCGAGCTTACTGTGAAACGTGTTGGGAATGGCAGGAGGCACATGAGCAAGGAATGGACCAGGATAAAAACCCAATGCCTGCCCC
AGTTGACCACCAGATATGCCCTGCTGTGTGTGTGCTGATGAAATTATCTTTTGATGAAGAACACAGACATGCAATGAATGAACTTGGTGGACTGCAGGCT
ATTGCTGAGCTTTTGCAAGTAGATTGTGAAATGTATGGACTTATTAATGACCACTATAGTGTTACATTGCGGCGATATGCAGGGATGGCCCTGACAAATC
TCACTTTCGGAGATGTAGCTAACAAGGCCACACTGTGCTCAATGAAAAGCTGCATGCGAGCTCTCGTAGCTCAGCTCAAATCTGAAAGTGAAGATTTACA
ACAGGTTATTGCAAGTGTTTTAAGGAATTTATCCTGGAGAGCAGATGTGAACAGCAAAAAAACCCTTCGAGAGGTGGGAAGCGTGAAGGCCCTTATGGAA
TGTGCTCTGGATGTTAAGAAGGAGTCCACTCTAAAAAGTGTGCTAAGTGCCTTGTGGAATTTGTCAGCTCACTGCACTGAGAATAAAGCAGATATCTGCT
CTGTTGATGGGGCATTGGCGTTTCTGGTGGGCACATTAACTTACCGAAGTCAAACCAATACTCTGGCCATCATTGAGAGTGGAGGTGGAATACTACGTAA
TGTGTCCAGCTTGATTGCTACTAATGAAGACCACAGGCAAATCCTAAGGGAGAACAACTGTTTGCAGACCTTACTACAGCACTTGAAATCACACAGTTTG
ACTATTGTAAGCAATGCCTGTGGGACCCTGTGGAATTTATCAGCACGAAATGCAAAAGACCAAGAGGCCCTCTGGGACATGGGAGCAGTCAGCATGCTCA
AAAACCTTATTCATTCAAAGCACAAAATGATAGCTATGGGCAGTGCTGCAGCTTTAAGAAACTTAATGGCAAACAGGCCTGCAAAATACAAGGATGCCAA
TATTATGTCCCCTGGCTCAAGTGTGCCATCTCTTCATGTTCGGAAACAGAAAGCTTTGGAAGCAGAATTAGATGCACAGCATTTGTCTGAAACATTTGAC
AATATTGATAATTTAAGCCCCAAAACCACCCACCGCAATAAGCAACGGCATAAGCAGAACCTGTGCAGTGAGTATGTTCTGGATTCAAGCAGGCATGATG
ATTCGATTTGCCGCTCAGACAATTTTAGCCTTGGTAATTTGACTGTTCTTTCTCCATATATTAATTCAACTGTGCTACCGGGATCATCTTCACCTCGACC
AACTATGGAAGGTAACAGGCCTGAAAAAGACAGAGAAAGAACAGCAGGACTTAGCAACTACCATTCTGCCTCTGAAAATTCAGGAAACTCCTCGAAACGT
ATTGGTGTACAGTTGTCAACTACAGCTCAGATTTCTAAAGTTATGGATGAGGTATCAAATATTCACATGGTTCAGGAAGACAGAAGTTCAGGGTCTGCTT
CTGAAATGCACTGCATGTCAGATGAAAGAACTTCACAAAGGAAACCTTCTAGTAATCACCCTCATTCAAATCCATTCAGTTTTGCTAAAGGAGATAGTTC
CAACAGGGGGTGCCCTGTAGCATTTATGAAGATGGAATACAAAATGGCATCTAATGACAGTTTAAACAGTGTCAGCAGCACAGATGGGTATGGGAAACGT
GGCCAAGTGAAGCCATCTGTAGAGTCTTACTCTGAAGATGATGAAGGTAAATTTGGTAGTTATGGACACTATCCTGCCGGCCTAGCACACAAAATTCACA
GTGCAAATCACATGGATGATAACGATACAGAACTAGATACACCAATAAATTATAGTCTGAAATATTCAGATGAGCAATTGAACTCTGGTAGACAAAGCCC
ATCACAGAATGAGAGGTGGGCAAGACCAAAGCACATCATAGACAGTGAAATGAAACAAAGTGAGCAGAGGCAGCCTAGAACCACCAAAACAACATATAGT
TCATATACTGAAAAAAAAAAAAAAAAA
5' EST: CX924562 [+]cgatcaggaggcaggatggagagctccggagccatggtgctgctgctgatggggcctgggggtagcccagcatggctgctgcttcgtatgaccagttgtt
aaagcaagtggcggcactgaagatggagaatacaaaccttcgacaagaattagaagacaattccaatcatcttacaaaactagagagtgaggcatcaaac
atgaaggaggttctcaagcagctacaaggaagtatcgaggatgaggcaatggcttcttctggccagattgatctgctggagcgtcttaaagaactaaatt
tagacagcagtaacttccctgcaggcaagactaggccaaagatgtctatgcgttcttacggaagcagggaaggctctctctctggtcattcgggtgaatg
cagtccggtccctgtgggttcctttcaaaggagaggccttttaaatggaagcagagaaagtactgggtatctggaagaacttgagaaggaaaggttatta
cttattgctgagcatgaaaaggaagaaaaagaaaagcgctggtactatgcccaacttcagaaccttaccaagaggatcgatagtctcccacttacagaaa
atttttctctgcagactgatatgaccagaaggcagctagagtatgaggcacggcagatcagagctgcaatggaggagcagttgggaacatgtcaggacat
ggagaagcgagtacagacaagagtgggcaaaatc
3' EST: CX924561 [+]cagtatatgaactatatgttgttttggtggttctaggctgcctctgctcactttgtttcatttcactgtctatgatgtgctttggtcttgcccacctctc
attctgtgatgggctttgtctaccagagttcaattgctcatctgaatatttcagactataatttattggtgtatctagttctgtatcgttatcatccatg
tgatttgcactgtgaattttgtgtgctaggccggcaggatagtgtccataactaccaaatttaccttcatcatcttcagagtaagactctacagatggct
tcacttggccacgtttcccatacccatctgtgctgctgacactgtttaaactgtcattagatgccattttgtattccatcttcataaatgctacagggca
ccccctgttggaactatctcctttagcaaaactgaatggatttgaatgagggtgattactagaaggtttcctttgtgaagttctttcatctgacatgcag
tgcatttcagaagcagaccctgaacttctgtcttcctgaaccatgtgaatatttgatacctcatccataactttagaaatctgagctgtagttgacaact
gtacaccaatacgtttcgaggagtttcctgaattttcagaggcagaatggtagttgctaagtcctgctgttctttctctgtctttttcaggcctgttacc
ttccatagttggtcgaggtgaagatgatcccggtagcacagttgaattaatatatggagaaagaacagtcaaatta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: