XB-CLONE-3576835
Clone Name: IMAGE:7695800
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Gene:
ppfia1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166237 [+]
ccgggacgagtgaggcggcctgacggagcccgggatactggagagcggagctccgcccctcagctctccccgggataccggtgtggccggcaggagacac
gggagccgctgaggattaaaccctgagctgcgcttcgtgcggttcccggagtcctgccgctgctttgggtacctgcctgccggtgcggggctcctacact
cacagaccgggtctgagctgcacaattctcactcccgggatcaagacacggcggcagcaagatgatgtgtgaagtgatgccaacaattagcgaggctgag
atgccatccgggggcaatggaggccatggctccgggtctcctttacaggcagactccgattctcattttgagcagcttatggtgtccatgttggaggaaa
gagaccgactgctcgacacactgcgggaaacccaggaatctctagcattgagccagagcaagttccaggaagccagccatgagcgggactctctccagag
gcagctgaacacggcccttccgcaggagtttgccgcacttacaaaagagctaaatatttgccgagagcagcttctagagcgggaggaagaaatagctgaa
ctaaaggctgaaagaaacaatactaggctcttactagaacatctggagtgtctggtttcccggcacgaaaggtccctaagaatgaccgtggtgaagaggc
aggctcagtcccctgctggtgtgtccagtgaagttgaagtcctaaaagcactaaagtctctgtttgagcatcacaaagctttggatgaaaaggtaagaga
gaggttacgggtggcattagagagatgtagcttattggaagaagaactggctgtaacacacaaagagctgatgatttcaagagagcagaataatcagaag
aaaatattcaatgacggtacagccgatgtcaaccacgagcaagagaacgcacttaacgctaacgggaagagatcatcagattgctctataaatcataatg
aggacctatctaaagtgatggagctccaggatatcatagagaaacaaaccaaggagcaagttcaaatgaaggagcgcctggctgttctttccaaccgggt
aacagagctagaagaagacctcgatactgcaaggaaggacctcatcaaatccgaagaaatgaactcaaaacttcaacgggacgtacgagaggccatggcc
cagaaggatgacatggaagagagaatcaccactcttgaaaaacgctacctcgccgcacagcgtgaagccacctctgtgcacgacctcaacgataaacttg
aaaatgaaatcgctaataaggagtctgctctgagacagagtgaagagaagaaccggcaaatccaggagcggcttgagctggcggagcagaagctgcagca
gactctgaggaaagcagaaacactgcccgaggtagaagcagaactggcacagcgagtggctgctctttcaaaggcagaggagagacacggtaacattgaa
gaacgcttacgccagatggaatcgcagttggaagaaaaaaatcaggaactgcaaagggcccgacagagagagaaaatgaacgaagaacacaacaaacgcc
tctcggatacagtagacaaacttctctcagagtccaatgaaaggcttcagcttcatcttaaagagagaatggcttcgctggaagaaaagaactctctgat
ccgtgaagtagaaacggccaaaaagcttattgatgaaatccagcacgagaaggatcagctgctgctggatatagacaccatgaggtcggaaacagagcaa
gtcaggataagggcagcttcccttcatcacagccggcctcaccttggcagtgtgcccgacttccgtttcgctctggctccttcctccatgtctgatcccc
attcggactcctacagctcttctctagtcctgcgtaggcctcagaaaggccgtctggccgccttgcgggatgagccatccaaggtacagaccctcaatga
gcaggattgggagagggctcagcaggcgagcgttcttgccaacgttgcacaagcatttgagagcgatgtagacatttctgacatagaagatgacagagag
accatattgagttcagtggatctgctgtcgccctgcggccaggccgacgcacagacccttgctgtcatgctccaagagcagctggatgctatcaataaag
aaatccgattaatacaggaagagaaggagaccacagagcagcgagcagaggagattgaatcccgggttgggagtgggagcctagataatttgggccgttt
caggtccatcacttcaattcccccttttactggtggtacttctcttgccggatcctctcctcctggcagtggtcgctcaactccaagaagaataccccat
agtccagcccgggaagtggataggctgggtgtcatgacactgcctccaggtacgagagatgacagtcgggacgacaaggccacaatcagatgtgaaacct
ccccacccgcctcccccagatctttgcgtttgggcgcagtgcataaaggagccctgcacactatgagccatgaagacattcgggatattagaaattccac
aggctcacaggatggccaggtgagcaaccccagcagcagtaacagtagccaggactccctgaacaaagcacctaagaagaaggggatcaagtcatccatc
ggccgcctctttgggaaaaaagaaaagggccgtccagggcaaatgggaaaagaggcacttggaccaggtggcattccagagtttgagaactcttcgcagg
aatcccttgggctcagcaagctgggagggcagaccgagaagaaccggaagctacagaagaagcatgaacttctggaggaagctcgcaggcagggcttgcc
atttgcgcagtgggatgggcccacagtggtggtctggctagagctatgggttgggatgcccgcctggtacgtggccgcttgcagggctaatgtgaaaagt
ggtgcaataatgtccgctctgtctgataccgagatccagagggagatcggcattagcaaccccttgcaccggctcaaactgcgactcgccatccaagaga
tcatgtctttaaccagcccgtctgcccctgccacttctagaacgacgttggcctatggcgacatgaaccacgagtggattgggaacgagtggctgcccag
cttggggctgcctcagtaccgcagctatttcatggagtgccttgtcgatgccagaatgctggatcacttgactaagaaggatctcagggggcagctaaag
atggtggatagctttcacaggaacagtttccagtgcgggatcatgtccttgcggaggttaaactacgaccggaaggagcttgaaaggaaacgagaggaat
gccagaacgagataaaagatgtgctcgtctggagcaacgaacggctgattaattggctggagtccattggcctgaaagaatatgcaaataaccttctaga
aagtggggttcacggtgctctggttgccttagatgagaccttcgaccacaatacgttggctttgttattacagatccccacccagaacactacggcacgt
gcaatgttggagcgggaattcaacaaccttctcatcagaggaacagatagaagatttgacgatgatgatgataaaagctttagacgagcgccatcatgga
gaaagaaattccgaccaaaggacgttcgagggttaagcgccggctctgcagagactctccctgccaacttcagggtcaactcctccatgtcctccatgtc
ctccccttctatgcagccaaagaaaatgcagatggatggcaatgtgtctgggatacaaagactggattccgccacggtcaggacatattcctgctaacga
tgacttttgtttacccacactacttctacagatgattatgcaacgttggaacgcgacgtgatttcacatttccagacctgggagagatctcactacaaag
aaaaaaaacgaactattcagacactttgagatattttaatacagagtttttaattagcgattaaaatcctagttaggggaaaaaaaaaaaaaaaaaa
5' EST: CX925157 [+]ccgggacgagtgaggcggcctgacggagcccgggatactggagagcggagctccgcccctcagctctccccgggataccggtgtggccggcaggagacac
gggagccgctgaggattaaaccctgagctgcgcttcgtgcggttcccggagtcctgccgctgctttgggtacctgcctgccggtgcggggctcctacact
cacagaccgggtctgagctgcacaattctcactcccgggatcaagacacggcggcagcaagatgatgtgtgaagtgatgccaacaattagcgaggctgag
atgccatccgggggcaatggaggccatggctccgggtctcctttacaggcagactccgattctcattttgagcagcttatggtgtccatgttggaggaaa
gagaccgactgctcgacacactgcgggaaacccaggaatctctagcattgagccagagcaagttccaggaagccagccatgagcgggactctctccagag
gcagctgaacacggcccttccgcaggagtttgccgcacttacaaaagagctaaatatttgccgagagcagcttctagagcgggaggaagaaatagctgaa
ctaaaggctgaaagaaacaatactaggctcttactagaacatctggagtgtctggtttcccggcacgaaaggtccctaagaatgaccgtggtgaagaggc
aggctcagtcccctgctggtgtgtccagtgaagttgaagtcctaaaagcactaaagtctctgtttgagcatcacaaagctttggatgaaaaggtaagaga
gaggttacgggtggcattagagagatgtagcttatt
3' EST: CX925156 [+]cccctaactaggattttaatcgctaattaaaaactctgtattaaaatatctcaaagtgtctgaatagttcgttttttttctttgtagtgagatctctccc
aggtctggaaatgtgaaatcacgtcgcgttccaacgttgcataatcatctgtagaagtagtgtgggtaaacaaaagtcatcgttagcaggaatatgtcct
gaccgtggcggaatccagtctttgtatcccagacacattgccatccatctgcattttctttggctgcatagaaggggaggacatggaggacatggaggag
ttgaccctgaagttggcagggagagtctctgcagagccggcgcttaaccctcgaacgtcctttggtcggaatttctttctccatgatggcgctcgtctaa
agcttttatcatcatcatcgtcaaatcttctatctgttcctctgatgagaaggttgttgaattcccgctccaacattgcacgtgccgtagtgttctgggt
ggggatctgtaataacaaagccaacgtattgtggtcgaaggtctcatctaaggcaaccagagcaccgtgaaccccactttctagaaggttatttgcatat
tctttcaggccaatggactccagccaattaatcagccgttcgttgctccagacgagcacatcttttatctcgttctggcattcctctcgtttcctttcaa
gctccttccggtcgtagtttaacctccgcaaggacatgatcccgcactggaaactgttcctgtgaaagctatccaccatctttagctgccccctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: