XB-CLONE-3575336
Clone Name: IMAGE:7794500
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Gene:
fam222a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122950 [+]
CGCTGGCCCGGGCTGCAGCGTTTGCGAGGCGATCTCAGAAGCAGATCCCACATCTCTGCACTGTCGGATCGTTCCCCTTCTTTCCTGGTACCCTCTGCTT
GTATTTAAACGCTGTCCCGGAAAGGATTCTTCCTGTGTGGTAAATTGCACCAGCAGAAGACAGAGAGCTGCTGCCCGTACCGGGGATTGAAGCTTGTGCG
GAGCTGCGCTGAGGGGATCTGATATATGAGCATTCGGTGCGGCTCGGATGGTGCATCTCCTCCTCCTGCTGCTGCAGCGCTGAGACAAGTCGCACATCGG
GGACACTGGGAAGCTTTACCTCCTTCTGCTTCAGCCCCACACGCTAAATATTTATTCTCTGCGGAGAAATACACTGGATCTTCTGCCTCCTTCAATTTAT
CTGAAGGCTTTGTGTCGCTCTTTACCTACAATAACCTGCCCTGTGGAGTTTACAGGATTCATCGCCAGTTTGCCCCTTTTTTTCTATTTATTATTTAAAT
GAATGTTTTCGTGCCCCCCTCCCTTCTGTCTGCTGTCATATTGATATGAAAACACTGCCAGAGGCTCTCATATAAATGGAATTTCCCCTGACAAAAGGCA
CAGACGAGGTACACGGCCGATTTCTGTCGGGTTGAGATCTTGAAGAGTTCCTGTGGCTTTTTTTTTTATTATCAAAGGATTTTGTGCTGCTAATGTGATC
GATTTGGCAACTCCATCCTGTTAAAGCTTTAGAACAGTTCCTTTTAATTTATGGCTCATCATTAATCTGGATTGGTGCAAGCCTTCTGTCTAGTACAAAA
GTTGACACTTTTATGAATGGGGTATTTTTAAAGGCTGTAGTCACTGAAAGGCTGAAACTCCCATACTGATTCCTGTCAGATGATACATTGGATTTCCCTG
GCAGATAAAGGGATTTAATGGAATACTTCTGAAGATCTTATGGCTTGGATTAAGTTTTAGGGTTGTGCAAAAGACTTGATCGTTGCCATTGGACCCCAGG
AGCCTGCCTAGGAGAAACTGAAGATTTATGTGTGTCCACCTGCCCAGCTGACCACAAGACCATTTAACCATGTTGGCCTGTCTTCAGAGAACCCAGAATC
CCCCAGTGAAGCACGTCATATGTGCAAATAAGAGTATAGAGCCTCGAAAGTGTGAAACAGCAACGCCAATGCAGAGTGCTCGGTATCCCAGCCCAGCAGA
GCTAGATGCATATGCACAGAAAGTGGCTAATAACCCCTTGACCATTAAGATCTTCCCGACAAACATCAGGGTTCCCCAGCACAAGCACCTAAATCGGACT
GTGAATGGTTATGACACTACAGGTCAACGATACAGCCCTTACCCAATGCACACAGGTGGATATCAAGGTCTGCTGGCCATTGTGAAATCGGCTAGCAAGA
GCGTGGTAAAAAATCCGGAGGGTAAACGGACTAAAATATCTTCTGCACAGGCTGGTGTTGCTCCATACCCATTGTCAAGCACTTTAACTCAAAGCCAAAA
CTGCAATGGACAGCTAAGTTATCTTGGAAGTCAGAAGCATCTGGATGCACCAGTTCCTCCCAATGTCACAGTGGCAGCATCCGTCATTCCACTAAGCAGC
AGAAATTTGGCACTTCAACAGTCTAATTTGCCCTCTATCCAGAGTATAATTTACCAGATTAACCAGCAGTGCCAGGCTCAGGCCTCTCACCAGGCCTGCC
AAGGGGTAGTGGTTACGAATTCTAGTCCATCAAAGCAGGGAATGGCTAATGGCTTCACTTCCATGACCGGGAATGCTGCTGCCTACTCTGGGACTGTTTT
GCAAGACTGCAGAGGAGGCAGCGAACTTGCTCTGGGTTCTGCTCCTGTAGTAACTAAAACTGGATCTTACCAAGAAGGCATGGACTATCTGATTTGGCAA
CAGAAGCAGCAACAACTCCGAATCTATAGTGGGGGCAGTGGTGGGGGTGGGGCAATCAGCAAATCTCCTGAAGTTTGCCCTGGAGTGCCCCGCACTTATA
CTCTTGCTAGTGCTGTTGAAAAAGTCAGCTCCTCTCCTTTAAACTGTGTTGGCATGCATGGTAACTTTTCTGTCGGGCAGTATTTTGCCCCTCCTTGGAA
CAGCATCTTGGTCACACCCAACAGTGACTGTTACAATCCACAGGAACTTGCCAACGGGCACAGAGATTTGGGAGTACATCCTTCAGATGGGTTAACCAGT
ATCCCCAGCAAGACCCTTTGTAATACCTCTATACTAAGCAGTAGCCTTCAGTCTTTGGAGTATCTTATCAATGACATTCATCCACCCTGTATCAAAGAGC
AGATGCTTGGCAAGGGTTATGAAACAGTGTCTGTGCCAAGGCTTCTAGATCATCAGCATGCTCACATTCGTTTGCCTATCTACAGATAGAAAGCCCTGCC
CTTTTCTCTTATAAAGTGATCAATGGTACACTGGTTCTGCATACTAACTTATGCTATTTAGCTTGAAAAGGCATGTTTCATAAGGGCAGAGGGCAAGGCT
TTTGGATGGGTAAACTGCACCACCACCACCAGGAGTGCAGAGTACCAGGAGCACTCCAGTGGCCAAACTGGTCCATCTGTTCCAGTGTAAAGTGCACTGC
CAGTGCCCAAATCCCTCTGGCGCCTTATATGTTAATACAGATCTAGCCATTTTCTGTAAATGAAATGTAAGAGAATAGCATGGCCTACACTTGATTCCAT
CACTATGTCCTGGGCTGCTGTCATGGTGGGAAGCTGCCAGTTGTACTCACAGGAGTCTGAATAAAATCAGGTTCAACTCCCAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX929537 [+]cgctggcccgggctgcagcgtttgcgaggcgatctcagaagcagatcccacatctctgcactgtcggatcgttccccttctttcctggtaccctctgctt
gtatttaaacgctgtcccggaaaggattcttcctgtgtggtaaattgcaccagcagaagacagagagctgctgcccgtaccggggattgaagcttgtgcg
gagctgcgctgaggggatctgatatatgagcattcggtgcggctcggatggtgcatctcctcctcctgctgctgcagcgctgagacaagtcgcacatcgg
ggacactgggaagctttacctccttctgcttcagccccacacgctaaatatttattctctgcggagaaatacactggatcttctgcctccttcaatttat
ctgaaggctttgtgtcgctctttacctacaataacctgccctgtggagtttacaggattcatcgccagtttgcccctttttttctatttattatttaaat
gaatgttttcgtgcccccctcccttctgtctgctgtcatattgatatgaaaacactgccagaggctctcatataaatggaatttcccctgacaaaaggca
cagacgaggtacacggccgatttctgtcgggttgagatcttgaagagttcctgtggcttttttttttattatcaaaggattttgtgctgctaatgtgatc
gatttggcaactccatcctgttaaagctttagaacagttccttttaatttatggctcatcattaatctggattggtgcaagccttctgtctagtac
3' EST: CX929536 [+]gggaggtgaacctgattttattcagactcctgtgagtacaactgggagcttcccaccatgacagcagcccaggacatagtgatggaatcaagtgtaggcc
atgctattctcttacatttcatttacagaaaatggctagatctgtattaacatataaggcgcccgaaggatttgggcactggcagtgcactttacactgg
aacagatggaccagtttggccactggagtgctcctgggactctgcactcctggggggggtggggcagtttacccatccaaaagccttgccctctgccctt
atgaaacatgccttttcaagctaaatagcataagttagtatgcagaaccagtgtaccattgatcactttataagaaaaaagggcagggctttctatctgt
agataggcaaacgaatgtgagcatgctgatgatctaaaagccttggcacagacactgtttcataacccttgccaagcatctgctctttgatacagggtgg
atgaatgtcattgataagatactccaaagactgaaggctactgcttagtatagaggtattacaaagggtcttgctggggatactggttaacccatctgaa
ggatgtactcccaaatctctgtgcccgttggcaagttcctgtggattgtaacagtcactgttgggtgtgac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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