XB-CLONE-3574678
Clone Name: IMAGE:7698254
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Gene:
gtsf1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC129008 [+]
AAAACGAAGACAAACTGGAAAACTCTGCTGCCAAATCCACCAAGGAATGTTGAGGTGGAAACCAGATTTTATGACCCACAAGATCCAGAGAGGCTCCTGC
AGTGCCCATATGATTCAAACCACCAGATCAGAGCCTGCCGTTTCCCGTATCATTTGATAAAGTGCAGGAAAAATCACAATGATGTTGCTATGCACCTTGC
AACCTGTCCCTTCAATGCTCGTCACCTTGTTCCAAGAGCTGAACTCAGTCACCACATCTCAAGCTGTGATGATAAGAGCTGCATTGAACAAGATATTGCT
GGTGAGAAGAATTATTACCAAAGAGATGTAAGCCCTTGTAGATGGCAAACCCCACCATGTGATGAAGATTGGGACAAAGATCTGCAGAATGATAAAGCAA
CATTTATGTGGGGCATCCCTTCTTACCCTGTACCTAGCAGTTCTGGAACAAACATATTGATGGATCCTAAAAACAGCCTTGGTTCAAGCCTACGTGCTCC
TAAATCGCTACCTTACATTTTGCCTTGGAAGATGCATACAAAGAGAGACTGATTTAATCCAGTTAATATCCAAAGACGAAGGTTAAAAGTTCTTCACTTC
ATTATGCACTGCGTTTGCGTTAACCTAAGAGTTCAATTTGTTGACCTAACTGAGCTGGGGAAACAGCATCTGGTTTATAAAAATGCTGAGAAAAAAAAAA
TATGCAAATAGTGATGGTTTGAGTGACTCACTTCTAAAACGCACATAGCTTTTAATTATCTGTACCAACAAACACACTGCGCAGTGTAAACCTGTGTTTT
CTCGTATGTCCAGTGACATCTGAAGCCATTTAATTTTGCGTGCATGATTGGACAGGATCATTTAGAGGCCACCTTTTGCTAGAAATCAGTTTCCTGCACT
CCAGATGGAGGGGCGAAACCCAGTACTTCTACGAAAGAACCATCACTCCCACCAAGTAGGGAGGGGGGGGGAGGAAAGGTGCATCATAGTACTACTGTGC
GGGCTGTCAGCCTGCAGCTCTGGAGAATGACACATTTTTTCCACTAGTTGCCCTTTAATCTAGTACCCACATGCCAGGCTTATTAGTGCAGTAGTCCTGT
TCAGAGCAAAATTCTTAGAACTTTCTGAACCTCTGGCCAGTATTCCCAGGATCTGTGCATCCAAGATCTCCGGCACCAGTTATTGGAAGCTAACTTATAA
CTTGTGTACATTTTCGTTTTATTACCAGAGTGTAGCCCAGGGAGGCCATCTTTGTTTAAAGGGGAAGGAAAGGCTAGGTCACTTGGGGTTGCCAGAATGT
TAGGCACCCCCAAGTGACTTAGATCGCTTACCTTGTACCCTGGGCTGGTGCCCCTGTTAGGAGAAAAAAGCACCAGCCCAGGGTACCTGTAGGAAGCACT
TCCTCCTTCCTTTCTGTTCTGCCGGCGAAATCCATCGGCCGGCGCATGCGCAGTAGAGTGAAAAGCCGACTTTAATGTTTAAGTTCCACTATTCACTCTA
CTGCGCATGCGCGCGCAAGTGAGAAGGAAGGAGGATGCGATCGCTGCTACCCCGGGCTGGTGCTGTTCTTTACTAACAGGGGCACCAGCCCGGGGTCACT
TGGGGGTGCCTGATATTTTGGCGTCCCCAAGTGACTTAGCCTTTCCTTCTCCTGTAATGGTGTTAATATGCCATCATTACATCCTGCCCTCAATCTACAT
CATTTTAGGCAAAGCTTTAGCCATTCTGCTAGTAAAATATAAAAAAACAGTTGTAAGAATACATAGAATAAAGTTTCCATAATAATATAAATGCTATCAT
TTGCAAATATAGTTATGTATATGTTTTACGGATTTGGTCAGTATTAAAAGTTCTTCAGGATGTCACATGAAGTGGTATGTGAGATCTAAACTGCCATCTT
CCCTCTGTTGATTAACCTTTAAATAAAGTAATATTTTGATTCTAAAAAAAAAAAAAAAAAAAA
5' EST: CX930210 [+]aaaacgaagacaaactggaaaactctgctgccaaatccaccaaggaatgttgaggtggaaaccagattttatgacccacaagatccagagaggctcctgc
agtgcccatatgattcaaaccaccagatcagagcctgccgtttcccgtatcatttgataaagtgcaggaaaaatcacaatgatgttgctatgcaccttgc
aacctgtcccttcaatgctcgtcaccttgttccaagagctgaactcagtcaccacatctcaagctgtgatgataagagctgcattgaacaagatattgct
ggtgagaagaattattaccaaagagatgtaagcccttgtagatggcaaaccccaccatgtgatgaagattgggacaaagatctgcagaatgataaagcaa
catttatgtggggcatcccttcttaccctgtacctagcagttctggaacaaacatattgatggatcctaaaaacagccttggttcaagcctacgtgctcc
taaatcgctaccttacattttgccttggaagatgcatacaaagagagactgatttaatccagttaatatccaaagacgaaggttaaaagttcttcacttc
attatgcactgcgtttgcgttaacctaagagttcaattttgtgacctaactgagctgggggaacagcatctggtttataaaaaatgctgagaaaaaaaaa
atatgcaatagtgatgggttgagtgact
3' EST: CX930209 [+]agaatcaaaatattactttatttaaaggttaatcaacagagggaagatggcagtttagatctcacataccacttcatgtgacatcctgaagaacttttaa
tactgaccaaatccgtaaaacatatacataactatatttgcaaatgatagcatttatattattatggaaactttattctatgtattcttacaactgtttt
tttatattttactagcagaatggctaaagctttgcctaaaatgatgtagattgagggcaggatgtaatgatggcatattaacaccattacagngagaagg
aaaggctaagtcacttggggacgccaaaatatcaggcacccccaagtgaccccgggctggtgcccctgttagtaaagaacagcaccagcccggggtagca
gcgatcgcatcctccttccttctcacttgcgcgcgcatgcgcagtagagtgaatagtggaacttaaacattaaagtcggcttttcactctactgcgcatg
cgccggccgatggatttcgccggcagaacaganaggaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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