XB-CLONE-3573503
Clone Name: IMAGE:7698850
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Gene:
dipk2a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161119 [+]
cacgcccccctcccccaggcagttggcagaggctctccgagccgcctctttctcagtaagttgagagccagccgagctgggggaagtggagagccgcaca
gatcggggggaaacgagcagcagccgcgctaccgaactgggccagagctggcctctcacagcgccgctcccctgccagagcagggcggctgaggggagca
gccggggcgggggcgcagtgagcgtgggaccatgctgcgcctcgcctcgctgaagttcggccgattgttccgctatgccaaggtgctgttcgcggccagc
ctgctggtggtgatgcttctgaacacgcactccctgctctcctccttccagcgcaacgagctgacggaccgccgcttcctcagcctcaataagtgcccgg
cgtgttttggaaccagttggtgccggaagtttatgaacgggcagctgagcttcgaaggctgggggcggctgcggctgctggacttctttaatgtcaagaa
cgtccacttcgctcagtacggggagccccgggagggcagccggcgtgtggtgctcaagcgcctcggttccaaccacgaactgtccgagctggaccagcgc
atctgcaagaaagccaccgggcggccccgctgcgatctggtccaggccatgtacaagacagacttcgctcggctcaatggggacgtgcggctgctgaccc
ccgatgtagtggagggctggtccgacctggtgcattgcccctcgcagcggctcctggacaggctggtccggcgctacgcggagaccaaggactcgggcag
cttcctgcttaggaacctcaaggacacggaacgcatgcagctgctcctcaccctggccttcaacccggagccgctggtcctgcagagcttcccatctgat
gaaggatggccttttgcaaaataccttggagcctgtggcagaatggtggctgtcaattatgttggagaagaactctggagttactttaatgctccatggg
agaaacgagtagacttagcatggcagctaatggaaattgcggaacagcttacgaacaatgactttgattttgcactttatctcctggatgtcagcttcga
taactttgcagttggaccgcgggatgggaaagttatcattgttgatgctgagaatgtgttagtagcggacaaaaaactaataaagcaaaataaacctgaa
aactgggatgtgtggtacgaaagcaagtttgatgactgtgacaaggaagcttgcctatctttttcaaaagagattctttgctctcgggctactgtggacc
ataattattatgctatttgccagaacctcctttccagacatgccacctggcgtggcacctccggaggactacttcatgatcctccagcagaaatcgccaa
agatggccgccttgaggccttgctggatgaatgtgctaacccaaagaaaagatacggcagatttaaatctgcaaaagaactgcgtgaataccttgcacaa
ttaagtaataatgcgaggtagtctatactgtagttttggtttttttttaattatccgtataatgaaaggtttcaattagctggagcacgtacagttttat
tatactgctgtatatacagaagttaaggaaatttgatgtttttgtgaatgctgtgggagtgagcctaatggcaagccagtgtgccatgcagtcactttca
tatgatgtacgtgaacctatctaattaaactcactaaaactactatcgatttttctcactgattacctgtaaggtgggtcagatgagggtctccaggtag
ctggggataatagcctttcatagtttaattgattattcatgtgtcaaaacgttattgacttgacattacattagtatcacaatttttgtgaagctgatgc
ctttacttggaaagttcaagttaagacttctggcctgttttaaaggctctttctttgtcatttcatatttgtacctttaaacaccctgtttctcgataaa
gaaaaacttgagggtataaatatttttgttgcatcttatttccaattagtagatttcttggaatggtctacagtaaatatttgcttaaagggcagggttc
ccgtattttttctatatttttgtctgcagttttgggttttgatcttgctctgtatataatgttgaccatgcattaaagttcacttcttctttttggtaaa
aaaaaaaaaaaaaaaaaaaaa
5' EST: CX931396 [+]cacgcccccctcccccaggcagttggcagaggctctccgagccgcctctttctcagtaagttgagagccagccgagctgggggaagtggagagccgcaca
gatcggggggaaacgagcagcagccgcgctaccgaactgggccagagctggcctctcacagcgccgctcccctgccagagcagggcggctgaggggagca
gccggggcgggggcgcagtgagcgtgggaccatgctgcgcctcgcctcgctgaagttcggccgattgttccgctatgccaaggtgctgttcgcggccagc
ctgctggtggtgatgcttctgaacacgcactccctgctctcctccttccagcgcaacgagctgacggaccgccgcttcctcagcctcaataagtgcccgg
cgtgttttggaaccagttggtgccggaagtttatgaacgggcagctgagcttcgaaggctgggggcggctgcggctgctggacttctttaatgtcaagaa
cgtccacttcgctcagtacggggagccccgggagggcagccggcgtgtggtgctcaagcgcctcggttccaaccacgaactgtccgagctggaccagcgc
atctgcaagaaagccaccgggcggccccgctgcgatctggtccaggccatgtacaagacagacttcgctcggctcaatggggacgtgcggctgctgaccc
ccgatgtagtggagggctggtccgacctggtgcattgcccctcgcagcggctcctggacaggctggtccggcgctacgcggagaccaaggactcgggcag
cttcctgnctaggaacctcaaggacacggaacgcatgcagctgctcctcaccctgg
3' EST: CX931395 [+]accaaaaagaagaagtgaactttaatgcatggtcaacattatatacagagcaagatcaaaacccaaaactgcagacaaaaatatagaaaaaatacgggaa
ccctgccctttaagcaaatatttactgtagaccattccaagaaatctactaattggaaataagatgcaacaaaaatatttataccctcaagtttttcttt
atcgagaaacagggtgtttaaaggtacaaatatgaaatgacaaagaaagagcctttaaaacaggccagaagtcttaacttgaactttccaagtaaaggca
tcagcttcacaaaaattgtgatactaatgtaatgtcaagtcaataacgttttgacacatgaataatcaattaaactatgaaaggctattatccccagcta
cctggagaccctcatctgacccaccttacaggtaatcagtgagaaaaatcgatagtagttttagtgagtttaattagataggttcacgtacatcatatga
aagtgactgcatggcacactggcttgccattaggctcactcccacagcattcacaaaaacatcaaatttccttaacttctgtatatacagcagtataata
aaactgtacgtgctccagctaattgaaacctttcattatacggataattaaaaaaaaaccaaaactacagtatagactacctcgcattattacttaattg
tgcaaggtattcacgcagttcttttgcagatttaaatctgccgtatcttttctttgggt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: