XB-CLONE-3570767
Clone Name: IMAGE:7703399
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Gene:
msl2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157752 [+]
GTTATAAAAAGCTCTGTGAATATATCACTCAGACGCCACTGGCGCAGGACATTTTAAATACAGTTGAGTGTTCTGCAGACATTTTGGCTTTTCTTCCTGA
AGAGCCTCCTGTTGAGGAAAAACAGGAATCCACCTCAGAGCCACCCTCGCCTTTGTGTCGCTCGCATTCTCCATTACCAACTAAATCTGAACATGGATCA
GAACATGAAGCAACCATTTGTCAACTACCACAAGACAGACCTAGTATTGAGGCAGAGTGCTTGGTCTCTAATGGACTGCCCAATTGTAATGGGCTGTCTG
AGACTGAACTCCCTGTAAATATCCCCTCCCCCGAAAGCCCCAGCCCCATGGACCTCTGTTGCACAGCTGTTGACATTAAAACAGAAGACCTGTCGGAGAG
CTTAGATACTGTGTGTGATCCGGTATCCACTAGTGACTTGTGTGCCGCAGGACTTGATATTTGTGGCTATAACGATGATCTTAAAAGTGGTGGCCCGTTG
TTGCTGAGTGTCGAGGAAGTCCTGCAGAGCCTAGAGACCGTTTCAAGTACAGAGGTCAGTGACTGTGATTTGCAGCACAGTTTGGATACCACAGCTTCTA
ATGGGGCTTTCTTGGAACTCTGTCCCCAACCCCTAACACACACTGTTTTGCTGTCTGACAGCCATGCCCCAGCACATGGGATCTCCTGCACTACCGCCAC
CCTAAAAATGGTAAAGACTCATCGCAAGCGATCCCGTTCCGAAAGCGACAGTGAAAAGGTGCTTCCGTTACCAATTTCTAGCATCATACTTGGGCCAACT
CTGGGATCCCCTGCCCCTCTGTTACTGACACGGGAAAACAGGAACTTCTTGCAGCCAATGGCAACTGTCCCCAATGGGGGTAGCTCACCCAAAATCAGTA
AAACTATTCTCTTGTCTAATAAAAACCTGAAAAAGAACCCAGAGCACGGTCCTAAGAAAACCCATCAAAAATCCAAACCAGCCTTGCTGAAAACAAAGGA
CAAGGTAAAGGAAAAAATCCCCACCAATAATGTGGTGCCTGGAAGTCCCACCAAAACTGTATATAAAAAGCCACAGGAGAAAAAAGGCTGCAAATGTGGA
CGTGCTACCCAAAATCCAAGTGTTCTTACGTGCCGAGGACAGCGCTGTCCTTGCTATTCTAATCGCAAAGCCTGCCTGGACTGCATTTGCCGTGGCTGCC
AAAACTCATACATGGCAAATGGAGAGAAGAAGCTAGAGGCATTTGCAGTGCCTGAAAAGGCTCTGGAGCAGACCAGGCTTACTTTGGGCATTAATGTAAC
AAGCATTGCTGTGCGAAATGCCAGCACAAGCACCAGTGTCATCAATGTCACAGGTTCCCCTGTAGCGACCTTTTTAGCTGCCAGTCCCCACGATGACAAA
AGCTTAGATGAAGCTATAGACCTTAGATTTGACTGTTAAATGGGACAGCTATTCCCCGGCTTGAAGAAAGCAGGAGAATGGCAGCAGTTTTATGGCAGCT
ATGGATCTGTTTGGTTAACTTTGCAGGAGCTTCAGCATATAGGTCACTTGTATCAAGTGTGTTCATTGCTAAGTTATGTGTTCTTGTATGTGAGGGGGCA
GGGAGGGAAGGGTCAGTGGGGTGGGACACGAAAAGAGTTTGGTGATAAGAGAAATTGTAACCCTAAATTCTGTATGTCTATTTAAAAAAAAAAAAAAAAA
AAAA
5' EST: CX939656 [+]ttataaaaagctctgtgaatatatcactcagacgccactggcgcaggacattttaaatacagttgagtgttctgcagacattttggcttttcttcctgaa
gagcctcctgttgaggaaaaacaggaatccacctcagagccaccctcgcctttgtgtcgctcgcattctccattaccaactaaatctgaacatggatcag
aacatgaagcaaccatttgtcaactaccacaagacagacctagtattgaggcagagtgcttggtctctaatggactgcccaattgtaatgggctgtctga
gactgaactccctgtaaatatcccctcccccgaaagccccagccccatggacctctgttgcacagctgttgacattaaaacagaagacctgtcggagagc
ttagatactgtgtgtgatccggtatccactagtgacttgtgtgccgcaggacttgatatttgtggctataacgatgatcttaaaagtggtggcccgttgt
tgctgagtgtcgaggaagtcctgcagagcctagagaccgtttcaagtacagaggtcagtgactgtgatttgcagcacagtttggataccacagcttctaa
tggggctttcttggaactctgtccccaacccctaacacacactgttttgctgtctgacagccatgccccagcacatgggatctcctgcactaccgccacc
ctaaaaatggtaaagactcatcgcaagcgatcccgttccgaaagcgacagtgaaaaggtgcttccgttaccnatttctagcatcatact
3' EST: CX939655 [+]aaatagacatacagaatttagggttacaatttctcttatcaccaaactcttttcgtgtcccaccccactgacccttccctccctgccccctcacatacaa
gaacacataacttagcaatgaacacacttgatacaagtgacctatatgctgaagctcctgcaaagttaaccaaacagatccatagctgccataaaactgc
tgccattctcctgctttcttcaagccggggaatagctgtcccatttaacagtcaaatctaaggtctatagcttcatctaagcttttgtcatcgtggggac
tggcagctaaaaaggtcgctacaggggaacctgtgacattgatgacactggtgcttgtgctggcatttcgcacagcaatgcttgttacattaatgcccaa
agtaagcctggtctgctccagagccttttcaggcactgcaaatgcctctagcttcttctctccatttgccatgtatgagttttggcagccacggcaaatg
cagtccaggcaggctttgcgattagaatagcaaggacagcgctgtcctcggcacgtaagaacacttggattttgggtagcacgtccacatttgcagcctt
ttttctcctgtggctttttatatacagttttggtgggacttccaggcaccacattattggtggggattttttcctttaccttgtcctttgttttcagcaa
ggctggtttggatttttgatgggttttcttaggaccgtgctctgggttctttttcag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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