XB-CLONE-3570172
Clone Name: IMAGE:7706547
|
Gene:
XB5765667
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC154096 [+]
GCGCACATCCATGTAACTGGACACAGCGCCTCTTTAGTCAGTGTGTAAGGGAAAGGCATGCAGCGACGCCACGGATAAGTCCATCTGCCGTCAGGTCACA
GAGAAGATGGCGGGGCAAGGACAGAAGACACGGGCTGCTAAAAATCGAGAAAAAAGACCCTCAAGCCGCAAACGCTTAAAAAAGGGGGAGGTCCAAGATG
CAGTCTGGAACCCCAAGAGAAGAAAGAGGGAGGTAGAGAAAAAGAAAGCTGATATGCCAAGTACAGAGGAGGAGGAGGGAGGCACATTAGAAAGTCCCCA
AATAAGGAAGAATTCCAAAAAATTTGTGGGGGCACATGTGTCTATACAGGGTGGCATATGGAACGCGGTGCTAGTGGCCAGGCAGATTGGGGCCAGTGCC
ATAGGTATGTTTTTGGGGTCACAGAGGACCTGGAAAAGCAAGGCTCTTGATGACAAGGCAGCTGAACGGTTCCAAAGAACCTGCAAAGAGCTAGGATTCA
AGTCTCGCTATATCTTGCCACATAGTCCCTATTTGATGAATTTGGGCTCTCCTAAACCAGATGTGCTTCAGAAGAGTCAAGCTATGCTTGTGGATGAGTT
GCACAAGTGCCACCAGCTGGGTCTTACAATGTACAATATCCACCCTGGGTCCTGTGTTGGTGCTATGTCTGTCAGCAAGTGTCTGGAGCTCATTGCTGAT
GGTATCAACCTTGCCCACGCTCAGGTTCCAGGGGTCATTGTGGTCTTGGAGAATATGAGCTGTCAGGGTAGTACAGTTGGTGGTCGATTTGAGGAGCTGC
GTGGAATTATAGACCTTGTTGTGGATAAATCCCGCATTGGAGTGTGCCTTGATACATGTCATGCTTTTGCTGCAGGTCATGATCTCTCAAAGAAGGCTGG
ACTTGAGCACATGCTGGATGAATTTAATAAAGTTGTTGGCCTTTCCTTCCTTAAGGCCATACACCTAAATGATTCCCAAGGAGAGCTTGGCTGCAAGCTG
GATCGTCATGAAAACATTGGGCACGGTCACATTGGGTTAGAAGGTTTCAGGCATGTGATGAATGAGCCAAGATTTAATGGCATTCCCATGATACTTGAGA
CTCCCCTCAGCTCAGATGACCATGCAAAGGAGATAGAACTGCTGTATTCTCTGTGTGCAAAAGGTCAAAGAGCAACAAAGCCCAAGAGAAAGCGCTGATC
CCATCAGTCTCTTACTGCTGCTCAGGGCTTCCAACCCAAATATGTTACATTAGTTTATGGTTGGGCAGTCCCATTGGCTCAAAACATAGTCTTGGTTGTA
TGCATGATATGCTGGTCAGTCAGTTATACAGTGATGGTTAGTACCTGTTATAGAGGCTATATACAAATGGGAGAGGGGCCTAAATTGTTCCTAGTTGTTG
ATAAGAAAAAAAAAGTTAACAGTTGAAAAAGAGCCTGAAATGGGTTGGATCACACCCAGATCTAACCTGCCTCATGCTGCCATATTAAGTAGTTCTGTAC
TTATGAACCTATATAAAGGATAAGTAAACCTTAAAAAAGTTTAAAACAATTAAACATTGCTCAGGGCTTTTGTAACCACCCATTTCTAAGCTACTAAGCT
ATTTATTAACTGCTAATGTAATGAATCTTTGTAACGGTAGTGCCACCTGCTGGTTGGTTCCTGTGACTGTATGGTCGGAGAGATATTTTCAAAGGTAAAA
AGGAGAACTCTTCTGATGTTGCTCTGCTCAGGAAACCAATCATAAACCTCAGGTGACTTTTCCAATTTCTATCCTGAGCAGAGCAACATCAGAAAGGTAT
CTGTTTTACTTCTGAACTTGTATCTCTCCGACCATCCAGTCAGAGGAACTAGCCAGCAGTTGTCACTGTTGTTACAAAGGTCATTATCTTGGCAATTAAC
GGGGATCTAAAGGCAAAAAATAAAATCCCCTTTTCAGGCTCCTTTCTTATAAAGAAACCTTTCTCTAATATACTTTTATTAACAAAAATCTGTTGCATTT
TTATAAAAACAAAAATTGTCTGGATCTGAGGTGCCTGTAGATGCAAAGAGAGCAATTTGAATCAACATTTATGCCCTGAAATATATGGCCACAAAAACAT
TCCATTAAAGCAGAGCATTCTGTCATATTGTTGCTATGTAAAGGGTTTAGGTGGAGTTGTGGCATTTCCATACAAAGGTGGGAATTTAGATCAGTAAGGA
GATGTATCTGGAGGCTGAGCATTACTTGGGGACCCCCGGTGCTCCTGTGCTGTGCCTTTGCTTTGCACTTGTTCCTTTGTATCCAGTCTGCATTCTGCCT
ATTCAGTGTCGAGCTGTTCCTGCAATGCTATCGTTATACAGTATAGTGCAAAGTTCAATATACCGTATGTTTATAAAATTTGAAGTGTAAATTGTTTAGT
TGTGTTAATCTTTAGGTTGTAAAAATTAAAGATTTGACAAGAACTCACAGTTTAGTAAAAACTTTCTTTATATTATACTCTAAAAAAAAAAAAAAAAAAA
AAAAAAA
5' EST: CX958292 [+]cgcacatccatgtaactggacacagcgcctctttagtcagtgtgtaagggaaaggcatgcagcgacgccacggataagtccatctgccgtcaggtcacag
agaagatggcggggcaaggacagaagacacgggctgctaaaaatcgagaaaaaagaccctcaagccgcaaacgcttaaaaaagggggaggtccaagatgc
agtctggaaccccaagagaagaaagagggaggtagagaaaaagaaagctgatatgccaagtacagaggaggaggagggaggcacattagaaagtccccaa
ataaggaagaattccaaaaaatttgtgggggcacatgtgtctatacagggtggcatatggaacgcggtgctagtggccaggcagattggggccagtgcca
taggtatgtttttggggtcacagaggacctggaaaagcaaggctcttgatgacaaggcagctgaacggttccaaagaacctgcaaagagctaggattcaa
gtctcgctatatcttgccacatagtccctatttgatgaatttgggctctcctaaaccagatgtgcttcagaagagtcaagctatgcttgtggatgagttg
cacaagtgccaccagctgggtcttacaatgtacaatatccaccctgggtcctgtgttggtgctatgtctgtcagcaagtgtctggagctcattgctgatg
gtatcaaccttgcccacgctcaggttccaggngtcattgtggtcttggagaatatgagctgtcagggtagtacagttggtggtcgatttgaggagctgcg
tggaattatag
3' EST: CX958291 [+]agagtataatataaagaaagtttttactaaactgtgagttcttgtcaaatctttaatttttacaacctaaagattaacacaactaaacaatttacacttc
aaattttataaacatacggtatattgaactttgcactatactgtataacgatagcattgcaggaacagctcgacactgaataggcagaatgcagactgga
tacaaaggaacaagtgcaaagcaaaggcacagcacaggagcaccgggggtccccaagtaatgctcagcctccagatacatctccttactgatctaaattc
ccacctttgtatggaaatgccacaactccacctaaaccctttacatagcaacaatatgacagaatgctctgctttaatggaatgtttttgtggccatata
tttcagggcataaatgttgattcaaattgctctctttgcatctacaggcacctcagatccagacaatttttgtttttataaaaatgcaacagatttttgt
taataaaagtatattagagaaaggtttctttataagaaaggagcctgaaaaggggattttattttttgcctttagatccccgttaattgccaagataatg
acctttgtaacaacagtgacaactgctggctagttcctctgactggatggtcggagagatacaagttcagaagtaaaacagatacttttctgatgttgct
ctgctca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: